最新刊期
1984年第2期
- 摘要：随着荧光仪器技术的发展,荧光分析方法日趋完善。目前荧光分析方法已广泛地应用到药物分析、临床化验、医学和生物学基础研究等各个领域,分析对象有无机离子和化合物、有机化合物、氨基酸、蛋白质、酶、核酸、糖、维生素和甾族化合物等等。近年来,荧光分析法在针刺镇痛的神经化学原理研究中得到广泛应用。一、荧光分析法的基本原理和特点某些物质被一定波长的紫外光照射,分子吸收能量,由基态跃迁到较高的能级(激发Spectrophoto-fluorometry is now widely used to assay concentrations of the central monoamine neurotransmitters in brain, cerebrospinal fluid, blood and urine etc. This article reviews the recent main applications of the techenique in the studies on acupuncture analgesia mechanism: (1) cha- nges of contents and turnover rates of the central monoamine neurotra- nsmitters under acupuncture analgesia; (2) qualities (strengthening or we- akening) and sites of actions of the central monoamine neurotransmitters in acupuncture analgesia processes;(3) the interrelationship between the central monoamines and other neurotransmitters in acupuncture analgesia processes. The basic principle and specialities of the fluorometric analysis and the factors effecting the analysis are briefly described in the paper.813|0|0更新时间：2023-08-11
- 摘要：耳针麻醉目前已广泛应用于甲状腺手术,在对甲状腺癌患者施行颈淋巴组织整块切除(颈清扫)中,常因手术范围广、创伤大,顾虑效果不佳,而不愿采用。为此,我们收集本院在耳针麻醉下作颈清扫术15例,与同期普鲁卡因静脉复合麻醉下作颈清扫术27例作一比较,以进一步探讨耳针麻醉在颈清扫术中的应用价值。方法术前准备:常规作术时体位练习3～4天,每天适应1～2小时。术前用药:术前1小时常规应用苯巴比妥纳0.1克,阿托品0.5毫克。切皮前1515 cases of thyroid carcinoma were operated by formal standard radi- cal neck dissection under ear-acupuncture anesthesia. At the same time, 27 cases were operated under general anesthesia for comparison. Method of ear-acupuncture anesthesia: Operation position exercise was done 3～4 days before operation, 1～2 hours everyday. Points of ear-acu- puncture anesthesia were selected on the affected side for all cases. The following ear-points were applied: Jing, Jiao-gan, Fei, Neifenmi, or Shen. The needles were inserted 15 mins. before operation. The electric frequency of stimulation was 500 times per min. As a routine, Luminal 0.1g m and Atropine 0.5 mg were injected intramuscularly an hour before the incision of skin. 15 mins. before the operation Dolantine 50 mg (or Dolantine 1mg per Kg. body weight) was added intravenously. Among the acupuncture anesthesia group of 15 cases 4 were male and 11 female. The average age was 38.1 years old (18～60). The effect of acupuncture anesthesia graded according to the national standard was excellent (grade Ⅰ) in 7 cases (46.7%), good (grade Ⅱ) in 6 cases (40.0%), fine (grade Ⅲ) in 2 cases (13.3%), the poor (grade Ⅳ) was absent. The effective rate (the combined rate of grade Ⅰ & Ⅱ) was 86.7%. Pathological studies showed predominantly papillary carcinoma in both groups. There was no operation mortality. The follow-up information was achieved in all patients. The 5-year survival rates were 100%. Comparing with the general anesthesia, ear-acupuncture anesthesia may have the following valuable points: 1. Adjuvant drugs for the anesthesia were simple during the operation. The doses were small. Anesthetic managements were easier. 2. During this whole course of operation the blood pressure was fai- rly stable, the pulse rate comparatively stationary and the circulatory function not affected. 3. There were no influences both on the time of the operation course and on the dosage of transfusion blood. 4. Particular anesthetic nursing was not needed after the operation. Administration of food was earlier. Recovery was more quickly. 5. The complication of the operation evidently reduced and if any they were milder. Particularly recurrent laryngeal nerve injury and compli- cations of respiratory tract could be avoided. Because the patients are conscious during the operation and the patient's speech may be examined at any moment, the recurrent laryngeal nerve injury could be avoided. Because tracheal intubation was not used, laryngospasm, edema of larynx and infection of lower respiratory tract could also be avoided. In view of a small portion of patients presenting incomplete analgesic effect under acupuncture anesthesia, a small quantity of 1% Procaine localy was necessary in order to complete analgesic effect. The results indicated that ear-acupunture anesthesia was a safe and effective anesthetic method to be used in formal standard radical neck dis- section for patients with thyroid carcinomas. It had many advantages and did not affect completeness of the operation.733|0|0更新时间：2023-08-11
- 摘要：用垂体后叶素经耳绿静脉注射,造成家兔急性心肌缺血性损伤的模型。动物24只分成5组(即对照[5只],艾灸[5只]、电烙灸[5只]、电炉丝灸——红光[5只]、石英灯灸——白炽光[4只])于“曲泽”穴施灸。穴表温度42℃左右,持续10分钟。从注垂体后叶素开始30分钟内,定时观察心电图P-P间期变化。实验结果:艾灸与另外三种电热灸都能抑制实验动物模型的心率缓慢,与对照组之间存981|58|0更新时间：2023-08-11
- 摘要：针刺所引起的镇痛作用,是通过机体内因实现的一种生理性镇痛,近年来发现脑内存在以脑室周围及导水管周围灰质为中心,以内源性吗啡样物质为主要递质的内在镇痛系统,通过下行抑制通路,特别是中缝大核(NRM)阻断痛信息的传入,发挥镇痛作用。针刺镇痛是否也是通过此种中枢机制实现的呢?为此我们记录大鼠NRM的单位放电及其伤害性反应,以反映下行抑制活动的水平及伤害性信息被阻断的情况,观察电针的效应,并研究高位中枢对它的调控。所得结果如下: 1.NRM神经元一般对非伤害性刺激无反应,而大多数对伤害性刺激能发生兴奋、抑制、双相或兴奋抑制转化等型式的反应,其中以兴奋型居多。还注意到兴奋型神经元自发放电频率较低,而抑制型神经元放电频率较高,而且兴奋型抑制转化型神经元,其对伤害性刺激的反应也与其自发放电背景有关,这些事实表明各种不同类型神经元,也许就是同一类神经元,只是由于处于不同的机能状态而反应有所改变。 2.电针(EA)“足三里”可以激活NRM神经元使其单位放电频率增加,并使其伤害性反应降低。伤害性刺激(尾端)过强或重覆使用也可像EA一样可较长时间激活NRM的自发放电和抑制其伤害性反应,说明EA和伤害性刺激均可激活此负反馈的痛调节机制 3.腹腔注射吗啡或芬太尼可明确而稳定地抑制伤害性反应,但未见有激活NRM自发放电的效应。吗啡类药物和EA的效应均可被钠洛酮(i.P)所阻断。表明两者所引起的镇痛作用有某种类似的中枢机制,但也存在着某些差异。 4.电解损毁双侧PAG可减弱EA和芬太尼的效应,PAG微量注射钠洛酮也可阻断EA的作用,此外还看到刺激PAG也可明显激活NRM放电,并抑制其伤害性反应。表明EA可经PAG内释放的吗啡样物质,激活NRM而发挥下行抑制作用。 5.尾核和伏核损毁或微量注射钠洛酮,可减弱EA的效应,刺激两者可以激活NRM这一重要的下行抑制起源部位,而这种效应可被PAG微量注射钠洛酮所阻断,表明这两个高位中枢,可通过其本身及PAG内的吗啡样物质激活下行抑制环节NRM引起镇痛作用。807|0|0更新时间：2023-08-11
- 摘要：我们曾于狗中缝大核内微量注射纳洛酮和普鲁卡因证明中缝大核不仅参与电针抑制内脏痛,而且与电针抑制恶心呕吐等内脏牵拉反应有密切关系。本工作观察电针和电刺激中缝大核对孤束核神经元兴奋性的影响,以探讨中缝大核参与电针抑制恶心呕吐反应的中枢作用途径。实验在箭毒化人工呼吸下的16只大白鼠上进行。刺激电极位置按Pellegrino氏大鼠图谱(1979)于AP=-8.6毫米,RL=0,H=7.5毫米坐标处插入中缝大核。多管玻璃微电极按此谱在AP=-7.0～7.2毫米(闩后0.2～0.4毫米),RL=0～0.3毫米,H=0.2～0.7毫米范围内记录。刺激中缝大核的刺激参数为100/秒,3～4毫安,5分钟。实验完后分别在刺激处通以直流电(1毫安,30秒)并从玻璃微管的侧管内电泳2％Panta-mine Sky Blue(10微安负电流,20～30分钟)取脑作切片,检查电极部位。在16只大鼠上记录了孤束核中55个神经元的自发放电,电刺激中缝大核对孤束核神经元自发放电的影响多是抑制性的(32/55)(见表1)。在其中的29个神经元上比较了电刺激中缝大核与电针足三里的影响,发现电刺激中缝大核对孤束核神经元自发放电的影响与电针足三里的效应在绝大多数的神经元(23/29)上是一致的。而且多是抑制性的(12/23)。对单纯电针足三里反应不明显的8个神经元,如果在电针足三里同时电刺激中缝大核可使其中7个神经元自发放电出现明显的抑制。以上结果表明电刺激中缝大核对孤束核神经元自发放电的影响多是抑制性的,并与电针足三里有明显的协同作用。在另一实验中采用标记脱氧葡萄糖放射自显影观察到电针足三里能使中缝大核的兴奋性升高,糖代谢增加,并同时调节孤束核的兴奋性;使之不再因牵拉胃而升高。综合上述实验结果看来中缝大核参与电针足三里抑制胃牵拉反应的作用途径之一可能是电针先激活中缝大核,继而调节孤束核下段的兴奋性,包括增强电针足三里对孤束核细胞的抑制效应,以阻断牵拉胃而由迷走神经传入的冲动。1148|0|0更新时间：2023-08-11
- 摘要：大量资料表明,中枢许多核团存在对伤害性刺激起兴奋反应和抑制反应的两类神经元。本室以往的工作证明,丘脑束旁核同时存在痛兴奋和痛抑制神经元,通过两者的相互配合活动,共同调制疼痛的感受。另有文章报道,电刺激外周神经可使视前区神经元的电活动出现兴奋或抑制反应。为了解视前区痛兴奋与痛抑制神经元的关系,及视前区与束旁核两个神经元的同时电活动,本文用两只玻璃微电极同时记录视前区或视前区与束旁核两个神经元的放In 8 rats, 12 pairs of the simultaneous electric activities of pain-exci- tation neurons (PEN) and pain-inhibition neurons (PIN) in preoptic area were recorded with two glass microelectrodes. The results showed that PEN and PIN in preoptic area existed and acted simultaneously. In 25 rats, 27 pairs of the simultaneous electric activities of PEN and PIN in parafascicular nucleus and in preoptic area were recorded. Noxious stimuli simultaneously provoked the increasing discharges of two PEN and decreasing discharges of two PIN in parafascicular nucleus and in preoptic area. It is considered that, PEN and PIN in parafascicular nucleus and in preoptic area may take part in the integration of paim sensation simulta- neously.733|0|0更新时间：2023-08-11
- 摘要：针刺可以降低体温已经动物实验和临床实践证明,但其作用机理尚未完全清楚,特别是对体温调节中枢的作用报道甚少。本工作以家兔视前区-下丘脑前部(PO-AH)温度感受神经元自发放电为指标,观察电针对其放电活动的影响,探讨针刺对体温调节中枢的调整作用。方法家兔18只,雌雄不拘,体重1.7～2.3公斤,以氨基甲酸乙酯(1克/公斤)静脉麻醉。手术包括气管插管,小脑延髓池引流和开颅。手术后,兔头固定于立体定向仪上,Experiments were carried out on 18 rabbits under anesthesia of ura- thane. We recorded the single unit discharge of thermosensitive neurons with the glass micropipettes in the preoptic-anterior hypothalamus(PO-AH) of the rabbits, and observed the effect of the electro-acupuncture of the point "Dazhui" and "Quchi" on its unit discharge. The experiments showed that the electroacupuncture could increase the unit discharge of the majority (20/26) heat-sensitive neurons in the PO-AH, reduce the unit discharge of the majority (12/13) cold-sensitive neurons and had no changes with the temperature-insensitive neurons. The unit discharge changes the heat-sensitive neurons and the cold-sensitive neurons usually revive their autonomic frequency rates after stopping the acupuncture 5～8 minutes. We observed the unit of the excited effect on the electro-acupuncture in the heat-sensitive neurons. The unit discharge showed twice increase at the 11 th～12 th min. after stopping the electro-acupuncture. Its discharge rates were higher than the electro-acupuncture and lasted for 30.3 minutes(rang 11～82 minutes). The present results indicates that the electro-acupuncture could marke- dly have excitative effect on the heat-sensitive neurons and inhibitory-ef- fect on the cold-sensitive neurons.939|0|0更新时间：2023-08-11
- 摘要：甲七肽(met5-enkephalin-arg5-enkephalin-arg6-phe6-phe7)是1979年从动物脑内及肾上腺内提取到的一个内阿片肽。在脑内有它的神经原,释放依赖于钙离子的存在。甲七肽的阿片受体活性与甲啡肽(met7)是1979年从动物脑内及肾上腺内提取到的一个内阿片肽。在脑内有它的神经原,释放依赖于钙离子的存在。甲七肽的阿片受体活性与甲啡肽(met5-enkephalin)相似,但其镇痛效应(小鼠热板法)比后者强8倍左右。为此我们观察它与针刺镇痛的关系。雄性Sprague-Dawley大鼠,脑室内注射酶抑制剂Captopril和bestatin作为甲七肽及甲啡肽的保护剂。10分钟后电针双侧“足三里”和“三阴交”共10分钟,然后微微波827|22|0更新时间：2023-08-11
- 摘要：根据文献报道电针脊髓具有明确的镇痛效果,并观察到电针对脊髓背角神经元有抑制效应,临床资料证明夹脊穴治疗某些疾病解除疼痛有良好疗效,我们过去的实验观察,发现脊椎旁电针刺激具有镇痛效应,这些资料均指示针刺镇痛过程中,脊髓起一定作用。考虑到在脊髓表面或脊椎旁外加电刺激时,是否首先引起脊髓的功能状态发生了某种变化,从而表现出对伤害性刺激的反应减弱的现象,同时考虑到实验研究中常采用脊髓反射放电作The present work is an investigation of the effect of para-vertebral electro-acupuncture on reflex discharges of the spinal cord in toads para- lyzed with Tubocurarini chlorid. The electric stimulation of a single rectangular pulse (wave width 1 millisecond, intensity 2～4 volts) or a brief train of rectangular pulses (rect. wave width 1 millisecond; frequency 20 cycles/sec; intensity 2～4 volts; duration of the train 500 milliseconds) were applied to the central end of the spinal dorsal root of the last spinal segment. The reflex discha- rges of the ipsilateral ventral root of the same segment were also recorded. Two needles were inserted vertically about 2～3 mm deep and symmetrically on both sides of the spine at the level between the third and fourth spinal vertebrae for electric stimulation. The parameters of needling stimulation were as follows: continuous bipolar pulses, frequency 60 cycles/sec, inte- nsity 2.5～4 volts, duration 2 minutes. The reflex potentials before and after electro-acupuncture were observed. One hundred and thirteen experiments were performed on 25 toads. The following results have been obtained. Both the poly-synaptic reflex discharges elicited by single rectangular pulse stimulation and a burst of reflex discharges by a brief train of re- ctangular pulse stimulation showed obvious changes under electro-acupu- ncture. Eighty-three (73.45 %) out of 113 experiments manifested inhibitory effect; eleven (9.73%) strengthened; seven (6.2%) temporarily strengthened and followed by inhibitiont and twelve (10.62%) no effect. The above observations indicated that the effect of paravertebral electro-acupuncture on spinal cord activities showed inhibition primarily, which might be responsible for the analgesia of para-vertebral electro- acupuncture. However, the mechanism of this inhibitory effect deserves further study.879|0|0更新时间：2023-08-11
- 摘要：关由脑内γ-氨基丁酸(GABA)与电针镇痛和吗啡镇痛的关系,我们曾用不同药物从不同角度进行过研究。所得结果表明,中枢神经系统中GABA能系统具有对抗电针和吗啡镇痛的作用。为了进一步确定GABA的作用部位,本实验中采用GABA合成和释放抑制剂3-巯基丙酸(3MP)以降低GABA能系统的功能,采用GABA降解酶抑制剂γ-乙烯基GABA(GVG)和GABA受体激动剂异鹅羔胺(musc), 以加强GABA能系统的功能。将这些药物给大1094|37|0更新时间：2023-08-11
- 摘要：以辐射热-甩尾法测痛,电针小鼠“人中”、“承浆”穴,观察二价阳离子与镧系元素的相互关系,并着重比较镧系元素铽,错、EGTA、吗啡和电针镇痛的特性。实验结果表明,腹腔预先注射纳洛酮使脑室内注射Tb(+++)、Pr(+++)、Pr(+++)、吗啡或电针镇痛效果全部或部分对抗,此时伴有脑Ca(+++)、吗啡或电针镇痛效果全部或部分对抗,此时伴有脑Ca(++)水平增设;动物出现痛觉过敏。但纳洛酮处理不影响EGTA的镇痛作用和脑Ca(++)水平增设;动物出现痛觉过敏。但纳洛酮处理不影响EGTA的镇痛作用和脑Ca(++)水平。蛋白质合成抑制剂环己亚胺能降低Tb(++)水平。蛋白质合成抑制剂环己亚胺能降低Tb(+++)和Pr(+++)和Pr(+++)的镇痛作用,增高In the present work we have investigated the localization of the anta- gonistic effect of GABA on electroacupuncture (EA) and morphine analge- sia. EA and morphine analgesia were found to be augmented by icv inje- ction of 3-mercaptopropionic acid (3 MP, 1 μmol) which inhibited the synthesis and release of GABA but not by its intrathecal injection(5 μmol). Microinjection of 3 MP (0.4 μmol) into periaquaeductal gray (PAG) was also able to increase the EA and morphine analgesia. Muscimol (0.05 μmol), a GABA receptor agonist, and γ-vinyl GABA (0.1 μmol), an inhibitor of GABA degradation, were found to decrease EA and morphine analgesia when they were microinjected into PAG. These results indicate that GABA in PAG area may play a very important part in the antagonistic effect of GABA on EA and morphine analgesia.838|0|0更新时间：2023-08-11
- 摘要：关于针感现象已有很多调查研究,薛崇成研究了截肢患者经络感作现象,认为经络感传现象是针刺穴位引起的兴奋在皮层沿特定经络模式扩延的结果。何广新根据临床观察认为针感循经传导现象与幻肢感、蚁行感不是同一过程。有人认为经络感传的形成有其相应的中枢和外周的生理、病理生理学基础。因此进一步观察分析幻肢感、幻肢痛患者的针感传导现象具有重要的意义。方法与结果观察对象为截肢患者,共52例,共中52 Cases of amputation have been observed. Of 52 amputees, 12 cases are due to gunshot wound, and the rest cases are traffic or production tra- uma amputation. They are all adults except one child. Of them, 24 cases complain of phantom limb, 28 cases phantom pain (7 cases having both phantom limb and phantom pain), and 7 cases have neither phantom limb nor phantom pain. When needling the points near stump, the PSC reaches phantom limb only in 4 cases. While needling the point on the healthy limb corresponding to the point on the diseased side, the PSC reaches a site corresponding to that of the phantom limb on the opposite side in 28 cases, reaches a site corresponding to the stump on the opposite side in 13 cases, and stays around the needled locus in 9 cases. The results indicate that all phantom limbs are not evoked, and all phantom pain are not evo- ked either by needling the points near stump. Some factors affecting pha- ntom limb and phantom pain are discussed. Phantom limb is a sequela of amputation, and is a pathological phenomenon. Channel phenomenon is not a sequela of some disease, but an intrinsic physiological function. The exi- stence and its spacial static site of phantom do not depend on afferent input, yet the existence of the so-called "phantom channel" does depend on acupuncture. The word "phantom channel" can not satisfactorily explain the channel phenomenon. Just as limbs have its struncture in the periphe- ral, and its representation in the cerebral cortex, channels may also have its structure in the peripheral, and its representation in the cerebral cortex.877|0|0更新时间：2023-08-11
- 摘要：从往的实验表明,大鼠摘除垂体后,针刺镇痛效应明显减弱;摘除垂体后注射促肾上腺皮质激素(ACTH),针刺镇痛效应得到恢复。并认为ACTH恢复针刺镇痛效应的作用与皮质酮无关,从而提示,ACTH在针刺镇痛过程中可能具有中枢作用。因针刺镇痛与脑内5-羟色胺(5-HT)神经元及去甲肾上腺素(NE)神经元的神经传递密切相关,所以,进一步阐明垂体在针刺镇痛中的作用机制,显然是有意义的。本文采用摘除大鼠垂体的方法,观察对脑内5-There are a lot of evidences that brain 5-hydroxytryptamine (5-HT) and norepinephrine (NE) are concerned with acupuncture analgesia. The previous works suggested that the cause of decreased acupuncture analge- sa in hypophysectomized rats was probably due to the defect of central actions of pituitary ACTH. In this paper, we observed the effects of hypo- physectomy on the 5-HT, NE in the brain and acupuncture analgesia in rats. The interaction among ACTH, 5-HT and NE in acupuncture analge- sia had been further studied. Electroacupuncture was applied to the "Renzhong" and "Chengjiang" points. The pain threshold was measured with radiantheat tail-flick me- thod. Analgesic effects was expressed as the percentage of increase in the pain threshold after electroacupuncture. The brain content of 5-HT, 5-hy- droxyindoleacetic acid (5-HIAA) and NE was determined with fluorescence spectrophotometry. In 15～30 days after hypophysectomy, the baseline pain threshold increased markedly. Brain 5-HIAA levels increased by 28% (p<0.05), as compared with those of the control group. In hypophysectomized rats the acupuncture analgesic effect was only 13.07±10.62%, much less than that (58.51±10.6%) of the electroacupu- ncture control group and sham operation group (p<0.05). There was no marked increase in the brain 5-HT levels and its turnover rate, no ma- rked decrease in the brain NE levels, as compared with those of the ele- ctroacupuncture control group and sham operation group. After hypophyse- ctomized rats were given ACTH intraperitoneally (15 units/kg), the reco- very of acupuncture analgesic effect ensued, and the brain 5-HT levels and its turnover rate also increased markedly. The above results demonstrate that in the process of acupuncture ana- lgesia the central actions of ACTH, probably to some extent, are achieved by means of strengthening the activity of central 5-HT system.1077|0|0更新时间：2023-08-11
- 摘要：成年猫9只,应用辣根过氧化物酶轴突逆行追踪法,将辣根过氧化物酶注入中缝大核及其一侧邻近网状结构内,观察中脑中央灰质、Darkschewitsch氏核和Cajal中介核向中缝大核及其邻近网状结构的投射。结果表明: 1.中脑中央灰质两侧半均出现标记细胞,注射侧略多于对侧。标记细胞在头尾侧方向上散在分布,尾端逐渐减少,后连合平面处的中央灰质内均未见有标记细胞。以上说明中脑中央灰质有向中缝大核及其邻近网状结构的直接投射,一侧网状结构接受中央灰质两侧半来826|29|0更新时间：2023-08-11
- 摘要：在我们以往的工作中,已经观察到;对黄牛进行电针镇痛时,脑脊液内乙酰胆碱含量增加,停针后可持续30分钟以上。为了在临床上进一步证明针麻对外科手术病人脑脊液中乙酰胆碱含量的影响,探讨乙酰胆碱在针刺镇痛中的作用。我们选择神经外科采用针麻手术的病人,观察针麻手术中的不同阶段脑脊液内乙酰胆碱的含量变化。实验方法对13例针麻神经外科手术病人在手术前、后不同阶段脑脊液内乙酰胆碱的含量进行了测定。其中男9例,女4例。息颅脑内13 neurosurgical patients operated under acupuncture anesthesia were selected, and the contents of Ach in cerebrospinal fluid were tested at various intervals during operation. It was found: (1) After induction of acupuncture, incision of skin and incision of brain tissue, the quantity of Ach was apparently higher than that before needling, and there was a considerable significance through analysis of variance. (p<0.01). (2) To compare the Ach content in cerebrospinal fluid during opera- tion with that after induction of acupuncture, the concentration of Ach was decreased gradually with the lapse of time, but without statistical significance. (3) It is indicated that the analgesic effect of acupuncture anesthesia appears to be correlated intimately with the elevation of the Ach level in cerebrospinal fluid of the patients.773|0|0更新时间：2023-08-11
- 摘要：本文的目的是比较电针与硝酸甘油,心得安及酚妥拉明对急性缺血性心肌损伤的血液动力学的影响。结扎冠脉左前降支以造成心肌的急性缺血。所有药物均在结扎冠脉左前降支15分钟时经静脉注射。结果如下: 1.给硝酸甘油(20微克/公升)20分钟后,心率(HR)、AP、左室舒张期终末压(LVEDP),心输出量(CO)和冠脉血流(CBF)均增加。 2.给心得安(10微克/公升)45分钟后,HR、AP、CO和CBF均降低而LVEDP增加。946|13|0更新时间：2023-08-11
- 摘要：针麻手术的实践以及原理研究的逐步深入,证明针刺镇痛是多种因素综合作用的结果,包括神经系统,中枢神经递质,体液因素等的参与,并起了重要的作用,但是要阐明针麻本质,提高针麻效果,不能忽视中医理论。为探索中医的辨证取穴与针麻效果的关系,应用生化指标,观察针麻胆囊切除术狗血中游离氨基酸谷氨酸(Glu)、色氨酸(Trp),酪氨酸(Tyr),与5-羟吲哚乙酸的变化。已知TrP为5-羟吲哚乙酸(5-HIAA)的前体氨基酸,5-HIAA为5-羟色胺(5-HT)的代谢产物,从实验资料表Expreiments were performed on 19 dogs, 12 of which were operated under acupuncture anesthesia. The effects of acupuncture anesthesia were graded as; excellent (1), good (2), poor (3), of these dogs 7 (58%) with excellent and 5 (41%) with poor effects in anesthesia. Serum free trypto- phan, tyrosine, glutamic acid and 5-hydroxyindolectic acid levels of 12 dogs during cholecystectomy under acupuncture anesthesia were determined by spectrophoto-fluorometric method. The results showed that the increase rate of 5-hydroxyindolectic acid in animals with excellent anesthetic effect during operation was higher than that in those with poor anesthetic effect. The increase was significant (p<0.05). It has been suggested that the change reflects one aspect of the acupu- ncture function. The change of free amino acid content in serum of dogs during chole- cystectomy under acupuncture anesthesia was not significant.1027|0|0更新时间：2023-08-11
- 摘要：本文为探讨心包经与心脏之间相对特异性的联系。采用家兔冠脉再灌注模型,从心电图ST_(11)、ST_(aVF)为指标,观察电针“曲泽”等不同经穴的效应。将56只家兔随机均分对照、“曲泽”、“隔俞”和“膝关”穴四组。用乌拉坦与氯醛精轻度麻醉,开胸、三碘季铵酚制动,人工呼吸,结扎冠脉左室支10分钟,各穴位组在结扎左室支后立即予以电针10分钟,连续脉冲波,频率60赫兹,负载电压5伏。927|37|0更新时间：2023-08-11
- 摘要：过去我们以血浆中促使去甲肾上腺素(NA)合成酶——多巴胺β羟化酶(DβH)活性,作为交感神经活动的指标,曾观察了交感神经活动与针麻效果的关系,针麻效果好者,DβH活性不变或略为降低,提示针刺能使交感神经在手术创伤的强烈刺激下保持相对稳定的状态。但是,DβH活性的高低只能间接反映血浆中NA的水平,而直接测定血浆中NA水平才能更直接地反映交感神经活动状态。同时既观察DβH活性,又测定血浆NA的水平,从两者的变化关系,更能Our previous clinical practice showed that acupuncture might stabilize the sympathetic nervous function. The changes of plasma DβH activity was in a lower level in grade Ⅰ cases of patients during subtotal gastre- ctomy under acupuncture anesthesia. In the present work the effect of acupuncture anesthesia on the changes of plasma NA level and DβH acti- vity was further studied on dog models during subtotal gastrectomy under acupuncture anesthesia. The experiments were divided into four groups: 1) subtotal gastrectomy was performed without acupuncture or any drug; 2) Operation was perfo- rmed under acupuncture combined with basal anesthetic drugs (Dolantine i.v. 1 mg/kg, Atropine 0.3 mg i.m. and some local infiltration with pro- caine); 3) Operation was done under acupuncture alone; 4) Acupuncture was made without operation. Blood samples were taken before operation, after induction and during operation. The plasma NA level and DβH acti- vity were determined with radio-enzymatic and spectrophotometric assay respectively. Before acupuncture NA level of dog plasma was 0.45±0.02 ng/ml, DβH activity, 23.8±5.13 nm/0.2 ml/h; NA/DβH ratio was 0.0188. In the group of only acupuncture without operation, the level of plasma NA was incre- ased to 126%, DβH activity to 91.1%, and NA/DβH to 136.2% after acu- puncture as compared to before acupuncture. In the group of operation under acupuncture anesthesia, NA, DβH and NA/DβH were increased to 188%, 110.8% and 155.9% respectively as compared to before acupuncture. In the group of operation without acupuncture or any drug NA, DβH,and NA/DβH were increased 305%, 110%, and 197.9% respectively. The plasma NA level in the group of acupuncture anesthesia is between that of the group of operation without acupuncture or any drug and that of the group of only acupuncture without operation. It was pointed out that the regu- latory function of acupuncture could inhibit the release of NA induced by the various surgical traumatic stimulation. A positive correlation presented between the plasma DβH activity and the change of NA level (r=0.335, P<0.02). When the level of plasma NA increased the DβH activity also increased. The NA level is used as the indication of the instantaneous activity of sympathetic nerve, while the DβH activity as the indication of the long period or continuous change of the function of sympathetic nerve. It is suggest that NA level and DβH activity could be used as an index of sympathetic nervous functional state of peripheral sympathetic nerve.945|0|0更新时间：2023-08-11
- 摘要：本实验用成年大白鼠12只,在定位器上将细玻针经小脑蚓部和中脑导水管水平刺入中脑导水管周围灰质前部。注入30％辣根过氧化物酶0.02～0.05微升或用微电泳法(3～4微安,5～7分钟)将10～15％辣根过氧化物酶导入。用四甲基联苯胺或二盐酸联苯胺进行显色反应。12例中有3例注射区位于中脑导水管周围灰质外侧部(包括内侧核和外侧核);3例注射区基本上局限于外侧核内;6例注射区局限于内侧核内。根据观察,得出如804|14|0更新时间：2023-08-11
- 摘要：1971年以来,我院外科急腹症研究组用中西医结合的排石疗法,治疗输尿管结石病,取得了显著的排石效果。在此基础上,我们对这一疗法的作用原理进行了逐项的研究。本文观察了电针狗的某些穴位对输尿管蠕动的影响,并对其机理进行了初步探讨。实验方法输尿管蠕动记录方法:采用两种方法。一是将水检压计连接于输尿管,观察输尿管蠕动时内压(以毫米水柱表示)的波动,藉以反映其蠕动活动的变化;另一是将一聚氯乙烯导管插入近膀胱处的输尿管,导管的另Experiments were carried out on eighteen anaesthetized dogs. Stimula- tion of the points corresponding to the human "Zhaohai" point (照海) and "Sanyinjiao" point (三阴交) with stronger interrupted waves or stimula- tion of "Renal" point (肾俞) and "Vesicle" point (膀胱俞) or unilateral ure- ter with stronger adjustable waves increased the ureteral peristaltic acti- vity. But stimulation of the "Zhaohai" and "Sanyinjiao" point with weaker interrupted waves or "Renzhong" point (人中) and "Yinjiao" point (龈交) with weaker and stronger interrupted waves did not change the ure- teral peristaltic activity.815|0|0更新时间：2023-08-11
- 摘要：用HRP顺行性轴浆运输技术,对8只猫上丘传出投射进行了研究。HRP注射至吻端上丘后,许多标记终未见于同侧外侧膝状体腹核腹内侧区、未定带腹侧部、丘脑后外侧核内侧份、束旁核以及中央正中核。丘脑背内侧核与丘脑网状核内标记终末较少,同侧二叠体旁核尾侧区、桥核背外侧区以及对侧付橄榄核背内侧区有最密集的标记终末。HRP注射至尾端上丘后,仅同侧桥核背外侧区有大量标记终末,二叠体旁核内标记终末较吻端上丘注射者777|12|0更新时间：2023-08-11
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