最新刊期
1986年第2期
- 摘要：胃肠平滑肌电活动的研究已近80年的历史随着弱电流放大技术的改进,这项研究有了迅速发展。但迄今为止,国内外尚缺乏一种性能稳定可靠的胃肠电专用生理放大仪,有关体表胃电快波的引导和放大技术尚未彻底解决。为此,我室和南京大学信息物理系,经过二年的试验性研究,研制了一台新型的NUV-3型低噪声光电隔离胃电放大器(简称NUV-3胃电放大器),并建立了一套动物及人体胃电活动的检测方法,现报道如下: 方法 (一)电极的制作胃电的引导电极,目前多采用银片和银This paper reports the results of fast and slow wave of GEG recorded from the surface of the body using a NUV gastroelectrical amplifier which was made by our laborafory recently. The experiments were performed in 59 normal human adults, 20 rabbits and 2 dogs. The results are as follows: In fasting condition, the slow ware was dominant, the frequency was 3 cycle/min in human, 3.5 cycle/min in rabbit and 5 cydle/min in dog; but only a few fast waves discharged. After eation, the amplitude and frequency of the fast wave increased si- gnificantly. In addition, we found that the result of GEG was influenced by mang factors, among which the recording and amplifying techniques ane of great impertance.820|0|0更新时间：2023-08-11
- 摘要：针刺麻醉从五十年代开展以来,已近三十年了,临床应用方面已经取得了不少的宝贵经验。针刺麻醉手术中如:甲状腺摘除、颅脑、颈椎前路骨科手术、肺叶切除、子宫全切除、输卵管结扎、剖腹产等效果都比较满意,并已通过了专家们的鉴定。但是,推广应用还很不够。我们认为开办专业学习班是个普及推广的好办法。希望有关单位也能象武汉同济医院口腔科那样,举办一些多种形式的富有成效的学习班,使具有我国特色的针刺麻醉在推广应用中不断得到提高,发展趋日完善。540|22|0更新时间：2023-08-11
- 摘要：电针刺激可以促进排卵,可以治疗排卵功能障碍性月经失调和不孕症,我们在电针后手部皮肤温度上升者针麻效果较好,和针刺可引起人体β-内啡肽在血中水平变化报道的启示下,曾报道电针促排卵的效果和体内雌激素作用水平及电针后手部皮肤温度变化(Hand Lempertuve changes after dec-tro-acupuncture、简称HTCEA),血β-内啡肽样免疫活性物质(β-Endorphin-like immunoreaactive substtamces,简称β-EpIS)变化有一定关系。本文对以上结果作进一步探讨。材料和方法Electroacupuncture(EA) is proved to be a method of ovulation induction. In present study, 11 chronically anovulatory patients (polydystic ovarian disease 9, hypogonadotropic amenorrhea 1, oligomenorrhea 1) were treated with EA in 13 cycles. On the 10th day of each cycle hand temperature(HT) after EA and blood β-endorphin-like immunoreactive substance (β-EIS) levels before and after EA were collected. 5 heslthy women with normal menstruation were recuited as controls. 6 of the 13 cycles treated with EA showed ovulation, while the other 7 cycles were failed. No regular change was seen in HT of the 5 onrmals. In the 13 anovulatory cycles, increased HT occurred in 6 cycles, of which 5 cycles showed ovulation after EA. 7 cycles showed decreased HT, and only one of them manifested ovulation after EA (p<0.05). Innormal cases, blood β-EPS leves before and after EA were fluctu- ater, and returned to preacupunctural levels at 30 minuter after withdraw of needles. The highest mean level was 193.94(±)144.04fmol/ml. In the 13 anovulatory cycles, basic blood β-EPS levels on the 10th day of thecycle were higher but not significant than that of the normoal women. After EA these blood levels of the 6 cycles resulting in ovulution after EAT either declined or maintained at levels at levels within the range of the normal cases, and blood levelds of the 7 cycles failing to show ovulation after EA were significantly higher than the normals' and throse that ovulated (p<0.05). Cycles which showed increase of HTCEA were more likely to associate with a declination of blood β-EPS after FA, but in cycles where HTC decreased, blood β-EPS levels elevatedafter EA. There appcars to be a negative correlation between the tendency of blood β-EPS changs after EA and HTC(p<0.01). HTC may be utilized to reflet the state of sympathetic activic. this study suggests that in many anovulatry cases the excessive sympathetic activity and hypothalamic-pituitary-ovarian disorder can be regulated by EA, monitoring of HTC may provide a rough but simple method for pre- dicting the effect of EA ovulation induction, and bllod β-EPS plays an important role in the mechanism of EA induction of ovulation.919|0|0更新时间：2023-08-11
- 摘要：姐妹染色单体交换(SCE)是检查致癌或突变因子的一种方法,此法灵敏、准确、快速。已知某些麻醉药或物理刺激可诱发突变或癌变。在研究某些作用于DNA (脱氧核糖核酸)的诱变剂或致癌物质。可在极低的,不能或很少产生染色体畸变的量就能诱发大量SCE,目前已把SCE列为检测环境中突变或癌变的可疑因子的方法,以往对于致癌物质的标准生物鉴定,大多用动物终身接触试验,但这种方法,时间漫长,人力物力浪费也很大,采用细胞遗传技术是较迅速的筛选试验,因为SCE能敏感地反映DNA的损伤和修复情况。实践证明针刺麻醉用于甲状腺手术具有The effect of acupuncture anesthesia (AA) for thyroidectomy on SCE RNA/DNA ration in lymphocytes was studied in 11 cases blood samples from peripheral vein were collected prior to AA (negative control), 3 hrs, and 6 days after AA and with mitomycin (positive control). One part of each sampl was subjected to Bre Drd-dye technique for detection of SCE, the other part for RNA/DNA ratios measured by CTAB method. From the results we may conclude that the phenomenan of mutation could not be induced by AA and SCE analysis may be considered a fairly good indicator for mutagenio activity in the study of AA. The quantitative ration of RNA/DNA in three groups were 11.033 (in pr- eanesthesia), 7.498 and 9.159 (3 hrs and 6 days post-AA, respectively). There were no significant differences amomg the RNA /DNA rations betw- een pre-AA and post-AA samples. The fact that either SGE or RNA/DNA ration did not show awy sign- ificant changes after AA provided strong support to the concept that acu- puncture is harmless in the respect of carcinogenesis.808|0|0更新时间：2023-08-11
- 摘要：大白鼠56只,共分四组,即①对照组;②电针组;③吗啡组;④吗啡+电针组。应用Sternbergel氏PAP免疫组织化学技术显示各组动物脊髓内P物质(SP),脑啡肽(ENK),及以Karnovsky-Roots氏组织化学方法显示乙酰胆碱酯酶(AChE),此外,还取已染SP及AChE的切片制作电子显微镜标本,作SP及AChE超微分布的观察,各种活性物质,并作了半定量测定及分析。电针后,在痛阈升高的同时,脊髓第Ⅱ层内SP的免疫反应性(LI)升高(P<0.001,与对照组相比),然而,与此相反在同一个部位内的ENK-LI降低(P<0.001)。注射吗啡后,SP-LI与电针组者相似;在第Ⅱ层内坛加(P<0.001),但注射吗啡动物若再进行电针,SP-LI不仅不继续升高反而降低(P<0.001,与吗啡组相比)。至于脊髓后角内AChE活性的组织化学改变,各组动物间互相差异,如:3.855±0.550(对照组),4.225±0.477(电针组),3.252±0.405(吗啡组)以及3.775±0.401(吗啡+电针组)。脊髓第Ⅱ层,不仅富于SP-LI,而且AChE也丰富、电镜观察表明“细”纤维轴终末内SP-LI见于清亮突触囊泡的周围,而由“粗”纤维(有薄层髓鞘者)AChE多分布于轴内。以上结果表明:在SP-LI改变方面,电针镇痛与吗啡镇痛的作用相似,但是在AChE活性的改变方面,二者作用相反,并认为:电针及吗啡分别对SP-LI及ENK-LI的影响可用Jessell-Iversen氏一级传入信息在脊髓内加工的模式来解释,同时,SP与乙酰胆碱之间的功能联系也不能排除。含AChE神经元可能影响SP的释放。1016|0|0更新时间：2023-08-11
- 摘要：针刺疗法是我国传统医学的宝贵遗产之一,有着几千年的医疗实践经验。针刺能够镇痛,引起了人们广泛的兴趣。自从1973年Pert和Snyder发现脑内存在有阿片受体后,把对镇痛本质的研究大大向前推进了一步。在研究阿片受体的基础上,进而寻找能和阿片受体结合的内源性配基,1975年Hughes等首先从猪脑中分离得到两个阿片样五肽——亮氨酸脑啡肽和甲硫氨酸脑啡肽。其后,又相继发现了β-内啡肽、α-内啡肽、γ-内啡肽、δ-Naga peptide is a new opioid tetrapeptide, which was found in human brain. We succesfully isolated it from the rabbit PAG. The perfusion technique was used for collecting sample of analysis. The perfusate was purified through a small column of Amberlite XAD- 2. The eluates were dried under vacuum. It was dansylated and chromatog- raphed on polyamide layer sheet. The fluorescent spot of DNS-peptide was eluted by methanol. Amino acids composition and sequence of the purified peptide were analysed by dansyl procedure and mass spectrome- try. It is shown that the structure of this peptide isolated from rabbit brain is Asn-Ala-Gly-Ala, which is coiacident with Naga peptide from human brain. The relationship between the Naga peptide and electroacupuncture analgesia was studied by the method of ultramicroanalysis and the technique of perfusion. The quantity of Naga peptide was estimated by scanning developed fluorescent spot on polyamide layer sheet on Hitachi MPF-4 fluorescent photometer. It was increased markedly about 4.80±0.98 nmole per milliter of perfusate (P<0.002, n=10)and the pain threshold of the animal was increased about 2.8 fold of the basal pain threshold. These results show that the Naga peptide exists not only in the human brain but also in the rabbit brain and it is related to acupuncture analgesia.1005|0|0更新时间：2023-08-11
- 摘要：中脑黑质致密部是多巴胺神经元中(Do-paminergic Neuron)比较集中的部位由此发出黑质纹体束主要通往同侧尾核、壳核和中央杏仁核。近年来有报道提示黑质纹体多巴胺系统可能有对抗电针镇痛和吗啡镇痛的作用。本文将海人酸(Kainic Acid KA)直接注入家兔黑质,观察黑质神经元被损毁后,对电针镇痛和吗啡镇痛的影响,并用连续切片、染色和荧光组织化学的方法,观察了神经元被损毁的情况。方法实验对象造体重为2～3公斤的家兔39只,雌雄未限。电针取穴为一侧相当“足三The effect of the substantia nigra (SN) injection of the neurotoxic agent, kainic acid (KA) 2μg/μl on electroacupuncture analgesia and morp- hine analgesia were studied in rabbits. The pain threshold was determined by the latency of withdrawal of head when the radiant heat was applied on the nose tip of rabbits. The point of electroacupuncture analgesia was "Zusanli" The dosage of morphine was 3mg/kg sc. The serial section of all experimental rabbit's brain was made. The KA destruction was observed with fluorescence histochemistry and toluidine blue stain. The results suggest that KA destruction of the DA ergic neurons in the pars compacta of SN enhanced the analgesic effects of electroacupuncture or morphine. Furthermore, the DA ergic neurons of SN might play an inhibitory effect on the analgesic effects of acupuncture or morphine. There is no correlation between acupuncture analgesia and morphine analgesia was found before intranigral injection of KA. But a positive correlation was shown between both analgesic methods after intranigral injection of KA. At 8-10 days time after KA injection, the cell bodies of DA ergic neurons disappeared and accmpanied by glial hyperplasia in the pars compacta of SN.863|0|0更新时间：2023-08-11
- 摘要：我们以往工作证明了针刺对蓝斑、缝际核、视上核及弓状核内乙酰胆硷酯酶有非常明显的激活作用。为了进一步了解针刺作用原理,我们企图进一步研究针刺对下丘脑中这些重要核团内与蛋白质、类脂类代谢以及膜渗透性调节有关的非特异性酯酶的作用,从而阐明针刺作用机制。方法本实验选用健康成年雄性大白鼠34只,随机分成针刺组(22只)、对照组(12只)。测表1 针刺对大白鼠痛阈的影响Thirty four healthy, adult and male rats were selected for this experi- ments, These animals were divided at random into acupuncture and the con- trol groups. The pain threshold were measured before decapitation. The cryostat sections were made for nonspecific esterase (NsEase) reaction and measurement with microphotometer. α- naphthol acetate and fast blue B salt was used to demonstrate NsEase in the brain section. 10(-1), 10(-1), 10(-5)M of E 600 was used as inhibitors of this enzyme. The results are as follows. 1) The influence of acupuncture upon supraoptic nucleus (SON) : The number of NsEase positive neurons and their reaction in SON of the acupuncture group were increpsed significantly than that of the con- trol. 2) The influence of acupuncture upon the ependyma of Ⅲ ventricle: At the level of media eminentia, The reactien of NsEase of acupuncture group was increased to significant level, as compared with the control. 3) The influence of acupuncture on NsEase of arcuate nucleus (Arc N): The reaction product of NsEase in the neurons of Arc N was faint brown yellow in color. There was no significant difference found between the acupuncture group and the control. This experiment indicated that acupuncture could activiate the NsEa- se of SON and ependyma of the Ⅲ ventricle. The mechnism of their actions and relations with other histochemical components under acupuncture anaesthesia were discussed in this paper.882|0|0更新时间：2023-08-11
- 摘要：r-氨基丁酸(GABA)是广泛分布于中枢神经系统内的抑制性神经递质,其在针刺镇痛中的作用早已为人们注视,但所获结果很不一致。有报道说明电针镇痛时脑内GABA增多,而细胞外 GABA含量的增多与针刺镇痛有关,但范少光等研究结果是脑内GABA能系统具有对抗针刺镇痛作用,可能与给药部位、途径及方法以及测痛方法等不同有关,并说明GABA的作用是复杂的,有待进一步研究。脊髓背角胶状质含有GABA能中间神经元,可与初级传入末梢形成轴-轴突触,也可与伤害感受种经元形成轴-树突触,脊髓水平的GABA可能在痛觉调制与针刺It is well known that many GABA-ergic interneurons are located in substantia gelatinosa and synaptically connect with the primary afferent terminals and projecting neurons, and they may play an important role in pain modulation. In the present study, the role of the α-aminobutyric acid (GABA) in the inhibition of spinal transmission of nociceptive info- rmation produced by acupuncture was observed by using iontophoretic bi- cuculline, an antagonist of GABA, at dorsal horn neurons of rats. Iontophoretic GABA-induced inhibitory effects on spontaenous disch- arges and nociceptive responses were partially reversed by iontophoretic bicuculline with ejecting current of 60 nA and 180 nA respectively. However, the inhibitory after-effect lasting for 20 minutes on the nociceptive responses of dorsal horn neurons produced by acupuncture app- lied at bilateral acupoints for 5 minutes couldn't be antagonized by ion- tophoretic bicuculline with current of 120-180 nA(n=10, P>0.05). In another series of experiments, immediately (15ms) after cessation of acupuncture for, 500 ms the nociceptive response was reduced to 77.5% of the control value, while the inhibitory effect of acupuncture on the nociceptive response did not occur if the bicuculline had been administe- red iontophoretically. It means that the immediate inhibitory effects of acupuncture can be almost completely blocked by iontophoretic bicuculline. It is of statistically significance (n=10, P<0.001). It is referred that GABA may be involved in the spinal segmental inhibition produced by acupuncture.1020|0|0更新时间：2023-08-11
- 摘要：电针能够纠正急性心肌缺血后心脏血液动力学的紊乱,增加心肌张力,调整收缩性能,促使心脏泵血功能好转;还能增加冠脉血流量,改善心肌氧供,恢复心肌的正常代谢,从而缩小缺血性损伤的范围。这些结果提示,电针在改善缺血心肌电稳定性,促使可逆性损伤心肌电活动向正常转化方面有一定的作用。本实验拟采用心肌单相动作电位(Monophasic Ac-tion Potential,简称MAP)为指标,观察电针“内关”对心肌缺血早期电稳定性及可逆性缺血性损伤心肌电活动的影响。1004|0|0更新时间：2023-08-11
- 摘要：临床与实验研究表明,针刺可缓解冠心病心绞痛,改善左心功能与输出量,对实验性缺血性心肌损伤时,有促进冠脉流量的作用,并可使早期坏死心肌减少。本实验采用冠脉再灌注的动物模型和电子显微镜方法,进一步观察针刺是否对早期缺血性心肌有促进恢复的作用,为探讨针刺对缺血性心肌的作用原理提供参考。方法实验使用健康雄性杂种家兔20只,平均体重为2.6公斤。除正常组外皆用成巴比妥钠麻醉(35毫克/公斤),经电动人工呼吸,沿左胸第三助间隙开胸,充分暴露心The animal model of coronary ligation followed by irrigation in acute ischemic myocardia had been used in this experiment. The results indicated that the untied ligation and the untied ligation plus acupuncture promoted the recovery of the oxygen-deficiency of my- ocardiac cells and nuclei and the recovery of the oxygen-deficiency of the cristae of the mitochondria had been promoted by acupuncture.Since acu- puncture can accelerate the recovery of the cristac and promote tho oxid- ative phosphorylation to synthesize the energy-rich phosphate bond and to produce adenosine-triphosphate(ATP), which provide the energy to muscle of heart, therefore the ischemic damage of the myocardia would be recovered more quickly.1000|0|0更新时间：2023-08-11
- 摘要：针感与大脑皮层的感觉整合机能密切相关。Marshall和Woolsey等早就应用诱发电位技术研究躯体感觉的皮层定位;近代出现的累加技术,更为体感诱发电位的研究提供了方便。本工作用猫,参照人体穴位的解剖位置,模拟穴位和非穴位区,以SI区皮层诱发电位为指标,探讨穴位反应的特异性。方法实验用20只成年猫,雌雄不分,体重2.1～4.2公斤。在氯醛糖(70毫克/公斤体重)麻醉下进行手术,暴露左侧大脑皮层后Many clincal and animal experiments have indicated that acupuncture can produce different effects at different acupoints. It Shows that the fu- nctions of acupoints are specificity. In the presentstupy the cortex evok- ed retponse elicited by stimulation of acupoints and nonacupoint was re- corded to inquire the relative specificity of acupoints. The experiments were performed on 20 adult cats anesthetized with a single dose of a-Chloralose. The concenrtric circles electrodes were used to stimulate the acu "Hegu", the "Wan'gu" and the nonacupoint that was between the two points. The parallel two electrodes were used to elicit SI somatosensory evoked potentials, using the computer ATAC-350 to average and recorded by X-Y recorder. Twenty cats were observed for 98 times. At the sme level local of cat's forelimb, during stimulating the"Hegu", all of the cats produced the evo- ked potential from opposite cortex, using the same stimulation on the nonacupoint that was beside 1cm to the "Hegu", all of the experiments can not get the evoged potential, but during stimulating the "Wangu" with the same stimulation, the evoked potentiel appeared again.inall of the cats. The cortex evoked patentials of "Hegu" were a biphasic positive-negative wave, peak-laiency 43.32±0.74 ms, amplitude 51.98±2.26uv duration 42.73 ±1.29 ms. The cortex evoked potentials of "Wangu" were also a biphasic positive-negative wave, peak-latency 49.40±1.41 ms, amplitude 44.57± 1.89μv duration 44.70±1.48 ms.These data show that the peak-latency of "Hegu" significantly differed(P<0.01)from the peak-latency of "wangu" and the amplitudes were also significative difference (P<0.05) between the two acupoints, but the duration show no signlficant difference (P> 0.1). These results suggest that the cortex evoked potential of acupoint "He- gu" point significantly differed from "Wangu" and the cortex evoked potentials were even more significative difference between the acupoints ("Hegu", "Wangu") and the nonacupoint. Consequently, there are rela- tive specificity not only between the acupoint and nonacupoint, but also between the two acupoints.1034|0|0更新时间：2023-08-11
- 摘要：循经感传现象是最常见的经络现象之一,研究循经感传的形成机理问题对进一步探讨经络的实质有重要的意义。目前对形成循经感传的特殊路线和规律的机理存在着“外周”“和“中枢”两种不同观点。因此,有必要对这个问题作进一步的研究。一般认为皮层诱发电位在一定程度上可以反映感觉的传入活动过程。本文对循经感传显著者在感传过程中的皮层体觉诱发电位的特点进行了观察,为探讨形成循经感传的机理及其与中枢神经系统的关系提供一些资料。1037|0|0更新时间：2023-08-11
- 摘要：耳与经脉相关是经络学说的组成部分之一。近年来,人们从经络现象方面研究耳与十四经脉的关系,做了大量工作。然而,值得提出的是耳穴诱发感传有无特异性,即刺激耳壳上临床常用的脏腑点所诱发的感传线,它是沿着相应的经脉循行(如耳穴心区诱发心经感传、胃区诱发胃经感传)或不相应的其他经脉感传循行,众说不一,报道互有差别。上海市耳针协作小组观察的耳穴放射感中,27条足太阳膀胱经属刺激耳穴坐骨神经者9条,其余是耳穴大肠、肺、内分泌等,没有显示明显的特异性;广西中医学院针麻经络研究室报告:104次测试中,In 12 propagated sensitive persons, we have observed 202 times of stimu- lation on the visceral regions of auricule and 138 times of stimulations on non-visceral areas to induce the P S C. It shows that the auricular point has no specific relation to the propagated sensation lines appeared, but still there are some rules and relation existes obviously most of propagated sensation lines run along the the 12 channels. On the other hand, a single auricular point may induce a lot of PSC on the 12 channels and different auricular points also induce the same line of PSC.Both di- splay a phenomena of coordination and combined with the function. PSC was imduced on auricular points to appear the same line or difference by repeated stimulation. Thus it expresses the variability and a relative stability in the function. The above may be valuable for detecting PSC and aurioular therapy913|0|0更新时间：2023-08-11
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