摘要:祖国医学很早就提到惊恐或愤怒等情绪引起心律失常,目前临床也对情绪激动或应激反应在心律失常发病中作用日益重视,我们以往工作表明,在家兔可建立针刺治疗实验性心律失常的模型,并对其作用机理作初步探讨。本文进一步观察延髓腹侧区在针刺抑制实验性心律失常中的作用。家兔58只,用乌拉坦(700毫克/公斤)和氯醛糖(35毫克/公斤)静脉麻醉,三碘季铵酚制动,在人工呼吸下进行实验。用电刺激下丘脑近中线区的方法诱发室性期前收Experiments were carried out on 58 rabbits anesthetized with chloralose and urethane. The animals were immobilized with gallamin triethiodide and maintained by artifical ventilation. Microinjection of morphine (5 g in 0.5-1μl) into the ventral medullary areas bilaterally decreased the num- ber of hypothalamic stimulation-induced ventricular extrasystoles (HVE). Inhibition of HVE by electrical stimulation of caudal raphe nuclei or by microinjection of L-sodium glutamate (0.5mM, 0.5-1μl) intc the same area were diminished by microinjection of naloxone into the ventral medullary area bilaterally. Inhibition of HVE by electrical stimulation of deep ocroneal nerve (DPN) was less obvious or even abolished by microinjection of naloxone (2μg 0.5-1μl) into the ventral medullary areas bilaterally. These results suggest that the inhibitory efedt of DPN stimulation on HVE is mediated by activation of opiate receptors in the ventral medul- lary areas via caudal raphe nuclei.
摘要:临床观察和动物实验证明,针刺有抗心律失常作用,临床发现冠心病病人常常伴有心律失常,而心律失常又往往是急性心肌梗死的早期重要合并症,因此研究心律失常及其针刺效应对进一步提高疗效有一定意义。而电针对慢心率作用的实验研究报道不多,本实验主要在大白鼠身上观察电针对慢心率的治疗作用,为探索针刺治疗慢心率机理的形态学研究提供实验依据。材料和方法选择成年、健康雌雄大白鼠54只,体重200~360克,要求每组实验动物性别相同,体重相近,实验分两批进行。第一批实验:共用大白鼠26只,其中电针组9只、非针组1054 healthy, adult rats were divided into 2 parts; the first part: 26 rats were randomized into electroacupunre (discontinuously), non-acupun- cture and control group.The slow heart rate of animals was caused by propranolol (0.1mg).Discontinuous electroacupuneture was applied on po- ints "Neiguan" bilaterally for 40 minutes (intensity 6-8 volt, frequency 50 HZ). The changes of heart rate of animals were observed before and the different time after discontinuous electroacupuneture, and comparison of heart rates between two groups. The second part:27 rats were randomized into electroacupuncture (continuely), non-acupuncture and control. The parameters and period of electroacupuncture were the same as that of the first part of experiment. The electroacupuneture was given to the rats in acupuncture group continuously for 40 minutes, the comparison of heart rate between acupuncture and non-acupuncture group was observed. The experimental results in the first part demonstrated that propranolol had the effect of decrearing heart rate; the heart rate before injection was 345.80±8.80; that of 10 min and 50 min after injection was 254.60±5.35 and 195.00±25.12 respectvely, there was significant difference between them (P<0.01). The heart rate before acupuncture was 266.89±5.39, and that after discontinuous electroacupuncture for 10 min was 305.11±9.28; the mean value of heart rate was incresed to 330.00±10.72 after discontinuous electroacupuncture for 40 min. The comparison between these two groups was staticanlly significant (P<0.01). The heart rate of rats in disconti- nuous electroacupuncture group was significantly increased as compared with non-acupuncture group (P<0.01); there was no significant differe- nce between electroacupuncture and control group (P<0.05). These resu- lts indicated that discontinuous electroacupuncture at points "Neigtina" had the recovery effect on slow heart rate. The experimental results in the second part were showed that the effect of propranolol on heart rate was the same as that of the first part. The effect of continuous electroacupu- ncture on slow heart rate was consistent with that of discontinuous elec- troacupuncture, and the recovery effect of continuous electroacupuncture on slow heart rate was better than that of discontinuous electroacupunc- ture. The results mentioned above indicated that: 1. The electroacupuncture at points "Neiguan" had the regulative. effect on heart rate under certain parameters of electroacupuncture. 2. The recovery effect of slow heart rate was better in continuous electroacupuncture group than that of discontinuous electroacupuncture group. 3. The regution of electroacupunccture on heart rats might be related to the regulation of vegetative nerve of heart for improving myocardial function.
摘要:我们在麻醉的家兔上观察到,刺激腓深神经可使压力感受性反射发生重调定,表现为压力感受性反射的敏感性增强,而对基础血压水平无明显影响。进一步的实验表明。在过深的麻醉条件下,刺激躯体神经对压力感受性反射的重调定作用变得不明显,提示麻醉剂能影响刺激躯体神经对压力感受性反射重调定的效应。根据梅俊等的报道,电针“足三里”和电刺激腓深神经能在脊髓引导到相同的诱发电位。故本实验试图在清醒家兔上,观察电针“足三里”对压力感受性反射的影响。实验在22只清醒家兔上进行。在局麻下Experiments were carried out on 20 conscious rabbits. Phenylephrine(20 μg/kg, i,v.) and sodium nitroprusside (30μg/kg, i.v.) were respectively used for changing arterial blood pressure. Arterial pressure and heart rate were: recorded simultaneously. The mean arterial pressure (MAP) heart rate (HR) regression line was constructed and the effect of electroacupun- cture at "Zusanli" (4 mA, 3 Hz, 0.5 ms, for 20 min) on baroreceptor reflex was observed. During electroacupuncture, no significant change in basal MAP and HR was observed but the slope of MAP-HR regression lines was steeper than that during control period, i.e, the baroreceptor reflex became more sensitive. In anesthetized animals, the compotind action potentials of the sciatic nerve were recorded. Electroacupuncture of the "Zusanli" acupoint was shown to excite the group Ⅰ, Ⅱ and Ⅲ afferent fibers in the sciatic nerve. These results suggest that electroacupuncture results in resetting of the baroreceptor reflex via activating the somatic afferents, thus enhancing the ability of baroreflex to correct abnormal blood pressure.
摘要:为了阐明针刺治疗冠心病过程中冠脉侧支循环功能,在戊巴比妥钠30毫克/公斤麻醉下,将颈动脉血液通过三通插管供给冠脉左前降支(LAD),关闭三通以阻断冠脉血运造成心肌梗塞,而后测定冠脉回返血流量(RF)和梗塞区冠脉压,并测量左室内压和股动脉压等指标,观察针刺双侧内关穴的效应,针刺组和不给针刺对照组各用犬15只,所得结果如下: 当梗塞15分钟心率、动脉压、冠脉压、跨越侧支血管网压力梯度、心内膜下区灌注压和心肌梗塞区及缺血区血管阻力和返回血流量仅有轻度变化,但左室舒张期终末For expounding the function of coronary collateral circulation during treating coronary heart diseases with acupuncture, the dogs were anaest- hetized with sodium pentobarbital (30Mg/Kg), the left anterior descen- ding branch of coronary artery (LAD) was supplied with blood from ca- rotid, through a three-way tube. When the three-way tube was turned off the myocardial infarction was occured by the blocking blood flow. The coronary return flow (RF), coronary pressure in the infarcted area, left ventricular pressure, femoral artery pressure were measured, and the ef- fects of acupuncture applied on Neiguan point and control 15 animls for each group were observed. The results are as follows: When the myocardium had been infarcted for 15 minutes, heart rate, artery pressure, coronary artery pressure, pressure difference of the col- lateral vessels, perfusion pressue in subendocardium and vessel resistance and RF in infarcted and ischemic myocardial areas were only altered, but LVEDP was increased. After the prolonged period of myocardial infarction, the most of indices mentionead above in control group were decreased, but vessel resistance and LVEDP in infarcted area were increased. All of them demonstrated that the myocardial infarction was serious, the number of closed anasto- motic branches of coronary collateral vessel was increased, so that the RF was decreased. Comparing with control group, artery pressure, coronary artery press- ure difference and perfusion pressure of the acupuncture group were inc- reased. However, the vessel resistance and LVEDP in infarcted and ische- mic areas were decreased. It resulted in the increasing of RF. It was sug- gested that the collateral circulation reserve in myocardial ischemic area could be mobilzed and the number of opened coronary vessel and the blood supply of ischemic area could be improved and increased by acupuncture.
摘要:脑血管疾病是严重危害人民健康的疾病之一,是老年人致残和死亡的主要原因。所以脑血管疾病的防治已引起国内外医学界的广泛重视,联合国世界卫生组织在75年召开了脑血管疾病防治专题学术会议。会议决定在世界范围内进行脑血管疾病防治方面的研究。根据首都地区调查表明脑血管病、心血管疾病和肿瘤,在目前是死亡率最高的三大疾病。祖国医学早在《内经》中即指出:中风之为病,如矢石之中人,骤然而至。”张仲景指出:“失风之为病,当半身不随”(《金医要略》)。说明该病发病急,变化快,临床症状明确,采用针灸治疗For studying the mechanism of therapeutic effect of acupuncture on the cerebral vascular diseases, dogs were anaesthetized with 30 mg/kg Pento- barbital sodium and ventilated by a respirator. Amimal model of cerebral infarction was set up after partial craniectomy of parietal and temporal bone, the left middle cerebral artery (MCA) was occluded. A number of blood gas indices of blood sample collected from carotid artery and supe- rior sagittal sinus were printed out directly with ABL-2 blood gasmeter. The blood samples of acupuncture group (n=12) and control group(n=13) were measured and recorded five times in this experiment respectively. Effect of acupuncture Renzhong and Hegu points on the metabolism of oxygen in cerebral were observed. The results are as follows: The difference of oxygen tension between artery and vein blood(Pa-vO_2 and oxygen absorption rate of cerebrum(A-v(Hb.1.39.SAT/A(HB.1.39.SAT) inc- reased gradually in the control group. However, Pa-vO_2 and oxygcn absor- ption rate of the cerebrum in the acupuncture group began to be reduced by acupuncture after 30 minutes. The difference of internal group and between groups by second 30 minutes of acupuncture are all remarkable (P<0.05). However, oxygen saturated degree of the blood (SAT) has not altered remarkablly both in the acupuncture group and control group. The results described above showed that Pa-vO_2 and oxygen absorption rate of the cerebrium could be reduced by acupuncture. It was suggested that the oxygen metabolism rate of the cerebral tissues might have been reduced by acupuncture.
摘要:脑血管疾病是严重危害人民健康的疾病之一,是老年人严重致残和死亡的重要原因。用针灸治疗脑血管疾病已有大量报道,取得明显疗效。为了探讨针刺治疗脑血管疾病的作用机制。我们采用结扎大脑中动脉造成脑梗塞的动物模型,观察电针对脑梗塞过程中脑血流的影响。方法采用健康杂种犬,体重14.82±0.67公斤,雌雄不拘,用戊巴比妥钠30毫克/公斤体重,在腹腔麻醉下切开气管并插管,联接3c-2型人工呼吸器维持正压呼吸,取左侧For stdying the mechanism of therapeutic effect on treating cerebral vessel diseases with acupuncture, the dogs were anesthetized with 30 mg/kg pentobarbital sodium and maintained with mechanical respiration The cere- bral infarction model of the animal was set up with partial craniectomy of parietal and temporal bone and occluding the middle cere bral artery (MCA). Carotid artery and vertebral artery were dissected. The vertebral artery was supplied with the blood from carotid. Cerebral volume of blood flow was recorded with MF-27 electromagnetic blood flow meter. Artery blood presstre was recorded and the blood of artery was obtained for analysis simultaneously. Acupuncture group(n=10) and control group (n=12)were recorded five times respectively. The values recorded 15 minutes after the ligation were used as the control. The weight of the brain was measured at the end of experiment. The results of experiment are as follows: 75 minutes after occlusion, comparing with the control the values of the ccrebral volume of blood flow of the acupuncture group reduced not remarkablly, there was significant difference between them (P<0.0l); the mean artery pressure (MAP) did not alter remarkablly; the resista- nce of the cerebral vessel (mmHg/ml/100gram/min, which equals MAP cerebral blood flow volume (ml/100g/min) increased retharkablly (P<0.05). Comparing with the value of control group, there was no remarkable difference in the practice measure valuer of the cerebral blood flow vo- lume, and the cerebral blood flow volume per one hundred grams brain tissues, MAP and the resistance and the decrease tendency of the cere- bral blood flow volume could be inhibited or alleviated by acupuncture. It was indicated that the cerebral vessel resistance could be decreased and the cerebral blood flow volume could be increased by acupuncture, so as to alleviate the brain tissues injury.
摘要:临床和实验研究结果表明,针刺内关可以治疗多种疾病。为阐明这种效应的机制,研究针刺信息的作用途径,一些作者对针刺“内关”的传入神经通路等进行了积极的探讨。我们曾观察过电针猫“内关”区的脊髓背根诱发电位节段分布范围,分析了内关传入途径与正中神经,尺神经和桡神经的关系,认为内关传入途径与正中神经关系最密切。本文以猫颈-胸导联叠加的ST段电位值变化为急性心肌缺血性损伤的指标,以正中神经复合动作电位为神经纤维类别兴奋的指标,分析电针“内关”效应的传In previous work, it was suggested that the median, ulnar and radial nerves were the composition parts of the signal afferent pathways exerted by electric stimulation "Neiguan". The median nerve might play an important role in this action. The purpose of this paper was to further study the relation between the afferent signal along the median nerve and the therapeutic effect in treatment of acute myocardial ischemia in cats, Experiments were performed in anesthetized with pentobarbital na- trium(35mg/kg), flaxedil immobilzed and artificially ventilated cats. Forty- nine experimental animals were divided into four groups: 1)cutting median nerve group; 2)group Ⅱ fiber excition; 3) group Ⅲ fiber excition group; 4) control group. The heart was exposed through a left thoracotmy in the fourth intercostal space. Applying the method of ligating the LAD coro- nary artery for 15 min,a state of regional myocardial ischemia was made. The ligation was then loosed and the ST values observed at 5′、10′、 l5′、20′、30′、40′、50′and 60′after loosening respectively. The median nerves were isolated at about 3 cm above the wrist Joint and the level of brachial-plexus in cats. The poteutial changes of the ST values of lead C-T ECG averaged 8 times by computer 7S06A were used as the index to evaluate myocar- dial ischemia and its recovery course. stimulation of electrical pulse for "Neiguan" point was given by the electronic stimulator SEN-3101 through isolator (0.2ms duration, 100 ms interval). During the experiment, first, in order to determine the stimulating intensity of electro-acup. at "Neiguan", inducing group Ⅱ or Ⅲ, fiber excitation, the compound action potentials of median nerves were recor- ded. Next, recording the ECG of C-T lead as control before ligation, LAD coro nary artery was ligated for 15 min, and then loosed slowly. The animals of 1-3 groups of 1-3 groups received the electric impulses on both "Naiguan", lasting for 20 min immediately at loosing the Jigation. The recording method and experimental procedure were the same in the control group except the electroacup. Folgwings are the main results of our observation: Theire was no statistical difference of ST values of lead C-T ECG between the four groups before ligation. Fifteen min after ligation, the ST values were elevated markedly, but there were no statistical signifi- cances between the four groups. According to statistical analysis with Ridit method, the result showed that there was a marked difference between Ⅱ,Ⅲ fibers excition group and the control group at all experimental processes, but between Ⅱ fibers group and control group only at 5-20 min; and no marked difference be- tween cutting median nerve and the control. The above mentioned results show that: 1) There was therapeutic effect of acute ischemic myocardial injury improved by electroacupuncture at "Neiguan" point in cats. 2) The median nerve might play an important role in the afferent pathway of the above mentioned effect, when group Ⅱ fibers were ex- cited the therapeutic effect appeared. When both group Ⅱ and Ⅲ fibers were excited, better effects of electroacupuncture at "Neiguan" point were obtained.