摘要:中枢神经递质在针刺镇痛中有重要的作用,近几年来的一些研究表明,中脑中缝核和导水管壁含有丰富的5-羟色胺,因此,探讨它在针刺镇痛中的变化,可为针麻作用原理的阐明提供科学的依据。考虑到微观放射自显影技术能够把组织的形态和代谢定位的动态过程统一起来进行观察,所以本研究用冰冻微观放射自显影和组织固定微观放射自显影平行地探讨了在针刺The role played by central 5-hydroxytryptamine(5-HT) in electro-acupuncture analgesia has been studied in rats by means of autoradiography with isotopic tracers 3H-5-HT. The purpose of the present study is to determine the localization of 3H-5-HT. The purpose of the present study is to determine the localization of 3H-5-HT in the midbrain raphe nuclei and in the mesencephalon aquaeduct as well as periaquaeductal gray matter. Parallel experiments were studied by freezing microautoradiographic method and histo-fixative microautoradiographic method. The analgesia effect of acupuncture can be enhanced or lowered by the increment or the decrement of the 5-HT level in the mibdrain raphe nuclei and in the mesencephalon aquaeduct as well as periaquaeductal gray matter. The results of the present experiments show that, when the rats were subjectad to electro-acupuncture analgesia. The miroautoradiographic intensities of 3H-5-HT in the midbrain raphe nuclei and in the mesencephalon aquaeduct as well as periaquaeductal gray matter. Parallel experiments were studied by freezing microautoradiographic method and histo-fixative microautoradiographic method. The analgesia effect of acupuncture can be enhanced or lowered by the increment or the decrement of the 5-HT level in the mibdrain raphe nuclei and in the mesencephalon aquaeduct as well as periaquaeductal gray matter. The results of the present experiments show that, when the rats were subjectad to electro-acupuncture analgesia. The miroautoradiographic intensities of 3H-5-HT both in the midbrain raphe nuclei and in the areas of mesencephalon aquaeduct were significantly increased. It may be observed that the release of 5-HT in these regions of the brain is accelerated during acupuncture analgesia. From this it can be concluded that the midbrain raphe nuclei and the mesencephalon aquaeduct as well as the periaquaeductal gray matter are closely related to acupuncture analgesia. The results imply that 5-HT in these areas may be one of the most important neurochemical agents mediating acupuncture analgesia.
摘要:大量资料提示,电针刺激可在中枢神经系统中释放阿片肽而发挥镇痛作用。近年来又发现,不同频率的电针刺激可激活大鼠中枢神经系统不同种类的阿片肽:低频(2Hz)电针促进脑啡肽释放,高频(100Hz)电针促进脊髓中强啡肽的释放。值得注意的是:有一部分大鼠电针不能引起镇痛效果,称为“电针无效鼠”,测定这些大鼠脊Influence of two novel enkephelinase inhibitors, Acetorphan and SCH34826, on the effect of electroacupuncture analgesia (EAA) has been observed in rats. Administered Acetorphan (2.0-4.0mg/kg, i.p.) or SCH34826 (30-100 mg/kg, p.o.) potentiated and prolonged the effect of 2 Hz EAA significantly in the nonresponder in which an evident analgesic effect can not be induced by 2 Hz electroacupuncture. This means that che non-responder of 2 Hz EAA can be converted into the responder with these two drugs. The non-responder of 100 Hz EAA, however, can not be changed into the responder with Acetorphan. These results again support our previous reports that enkephelin plays an important role in low frequency EAA. Meanwhile, this suggests us a probable route for potentiating acupuncture analgesia in clinical practice.