摘要:自1965年11月上海华山医院成功地将针刺麻醉应用于颅脑手术以来,全国神经外科针麻研究协作组经历了两个阶段协作攻关研究,共积累5,244例临床资料。其中前颅窝2,107例(40.17%),颞顶枕区1,951例(37.2%),后颅窝1,186(22.61%)。经反复修改制定了统一手术From March 1975 to February 1982 and from April 1987 to October 1990, the national cooperative neurosurgerical acupuncture research group had already accumulated the clinic data of 5,244 cases totally, consisting of 2,107 cases in frontal fossa, 1951 cases in the temporo-parieto-occipital region and 1,186 in posterior fossa. By the same manipulative procedures and scaling criteria, the indications, choices of acupoints, stimulus parameters, adjuvants, preoperative measurements, and physiological and biochemical changes during operations were studied. Practically, the results was not only reliable, but repeatative highly. 95% of the cases in frontal fossa belonged to grade Ⅰ(success),91.5 % of the cases in temporo-darieto-occipital region was grade Ⅰ and 89.38% of the cases in posterior fossa was grade I. We suggest that acupuncture anesthesia should be widely used as one of the usual methods of anesthesia. In this paper, the relative specificity of acupoints, the mechanism of adjuvants, personal differences and preoperative measurements were discussed. In the meantime, the advantages and the remaining problems of acupuncture anesthesia in craniocerebral operations were also mentioned.
摘要:中缝背核(NRD)在疼痛调节和针刺镇痛过程中起重要作用,并参与内分泌、情感、体温、饮食活动和睡眠与觉醒周期等多种生理功能的调节。电解损毀NRD可明显减弱电针的镇痛效果Nucleus Raphe Dorsalis (NRD) plnye an important role in acupuncture analgesia. The aim of this investigation is to determine the effects of electro- acupuncture (EA) analgesia on the ultrastructure of NRD. 12 wistar rats (220-250g) were divided into control and experiment groups. Pain threshold was determined by potassium iontophoretic dolorimetry. EA was applied at bilateral "Zusanli" points. The effective aealgesia animals and control animals were sacrificed and the NRD were taken out for electron microscopic observation. In EA analgesia groups, NRD was observed the number of the clear round vesicle-containing terminals and clear round vesicles with the granular vesiclecontaining terminals showed a significant decrease and sometimes vesicles were emptied. The area of presynaptic terminals wae expanded. Some synaptic gaps appeared narrow profiles. Part of the mitochondria and endoplasmic reticulum in neuron and neurogliocytes appeared the expanded profiles. These ultrastructural change of the NRD in EA analgesia might indica tet he neuronsa nd neurogliocytes were in active functional state.
摘要:近年来,大脑皮层参与痛觉调制逐渐受到人们的重视。Hardy发现刺激大鼠前额叶(prefrontal cortex,PFC)具有行为镇痛机能,并且观察到刺激PFC可以改变中脑某些神经元的电活动,这些神经The unit discharge of neurons in nucleus raphe magnus (NRM) were recorded with glass microelectrodes in 33 waking and tubocurarinc-immobilized rats. It is observed that effects of the noxious stimulation and prefronlal cortex (PFC) stimulation on spontaneous discharges of NRM neurons and effects of PFC stimulation on noxious-evoked discharges of NRM neurons by stimulating sural nerve. The results indicated that noxious stimulation and PFC stimulation could increase spontaneous discharge of most (68.18% and 60.82% respectively) NRM neurons. It was also noted that most (49/80) NRM neurons altered their spontaneous discharge in response to noxiouse stimulation and PFC stimulation in a similar manner. The remainder (31/80) opposite manners, PFC stimulation could alter the response of NRM neurons to noxious stimulation, PFC stimulation could enhance or reduce noxious-evoked discharge in NRM neurons which alter their spontaneous discharge in response to noxious stimulation and PFC stimulation in a similar manner, PFC stimulation could reduce noxious-evoked discharge in NRM neurons which alter their spontaneous discharge in opposite manners. The above results suggest that PFC and NRM have a functional relation, PFC could modulate nociceptive response and it might be partly responsible for influence on activily of neurons in NRM.
摘要:近年来研究证明脑内存在有以脑室周围灰质和导水管周围灰质为中心的镇痛系统。它可以通过延髓的下行抑制通路,阻遏脊髓背角痛信息的输入而起到镇痛作用。我们以往的工作多次证明电针“足三里”确实可以激活延脑中缝大核(NRM)这The experiments were performed on the rats. Unit discharges in NRM and their nociceptive responses to dental pulp and tail noxious stimulations were recorded extracellularly with glass microelectrodes. We observed that EA at the "Hegu" acupoint could increase spontaneous discharges of NRM neurons and inhibit their nociceptive responses to noxious stimulation acting on dental pulp and tail. It is interestingly noted that the inhibitory effect of EA on noxious stimulation applying on the pulp is stronger than the tail.
摘要:This paper deals with whether the posterior hypothalamus area(PHA) can receive signals from electroacupuncture(EA) at "Neiguan", and whether a relative specificity does exist between acupoints or acupoint and non-acupoint The neuron activity was recorded extracellularly for analyzing the influence of EA at different acupoints and nonacupoint on the discharge of PHA neurons.
摘要:冠心病心绞痛主要是由于冠脉血流减少,心脏血氧供应不能满足心脏的代谢需要而引起的。血氧供应不足时,心肌的代谢会发生一系列改变,酸性代谢产物增加,导致缺血局部pH值降低,严重者导致细胞内酸The experiments were performed on 30 healthy mongrel dogs,intubation was taken in the left anterior descending coroniary (LAD), the blood in carotid was tran-sported to LAD through a peristalic pump, the blood flow was reduced to 3-5tnl/min, thus acute myocardial ischemia was produced. Basing on this condition, 0.1-0.16ml bradykinin (2ug/ml) was gived into LAD coronary before recording to produce angina pectoris. The effects of electroacupuncture(EA) at "Neiguan" area on myocardial oxygen metabolism, pH of coronary sinus blood and myocardial contractile force were observed (EA intensity 5 volts, frequency 1-20 Hz). The results are as follows: 1. EA could reduce obviously A-V difference of blood oxygen capacity (Ca-vO_2) and the rate of myocardium extracting oxygen (O_2E), thus reduced obviously oxygen consumption of ischemic myocardium. 2. EA could reduce V-A difference of carbon dioxide partial pressure (PvaCO_2), prevent the decrease of pH of coronary sinus blood (PHv), this indicated that EA could prevent accumulation! of acidic metabolic products. 3. EA could increase myocardial developed tension (DT) of ischemic area, strengthen myocardial contractile force of ischemic area. Above results indicated that EA could reduce oxygen consumption of ischemic myocardium, prevent the decrease of pH of coronary sinus blood, thus myocardial cell acidosis was prevented, myocardial contractile force was strengthened. It might be the mechanism of acupuncture treating coronary heart disease.
摘要:国内有报道针刺能改善左心功能,改善心肌微循环和氧供,减轻心肌缺血程度并缩小其范围。针刺对室颤阈的影响,报道甚为罕见。本文观察电针刺激分属心包经及心经的“内关”、“灵道”穴位对大鼠The Neiguan and Lingdao were stimulated by electroacupunclure in anesthetized Waster rats. We observed that the value of ventricular fibrillation threshold (VFT) was increased significantly in 30min of electroacupuncturing (P< 0.01) and in 15min of stopping the acupuncturing (P<0.05). Although the value of VFT was increased until 30min of stopping acupuncture, it was not significantly different compared with its basic value. The control group were dealt with the same way as the experimental group except electroacupuncture. The value of VFT measured in control group were not significantly different compared with its basic value.
摘要:近年来,利用耳穴刺激治病在临床上已有了广泛的应用,在不少方面取得了较好的治疗效果。关于其机制的研究,虽也发表了不少的研究报导,但由于建立动物模型的困难,至今尚难有较大进展。1957年法国Nogier等提出倒置胎儿模型,当身体某部有疾患时在耳廓相应区域会出现压痛点或低电阻点,使研究推进一步。我国许多单位利用猴、家兔、犬等动物进行了耳廓压痛点及低电阻点的研究,李肇特等于1973利用穴位的低电阻这一特点,We have developed an animal model to study the effect of stimulating auricular points on the function of hepato-biliary system. The preliminary result shows that 14 of 18 rabbits acquired a marked increase (P<0.05) in the amount of hepatic bile seeretion 3 min and 7 min after point No, 3 stimulated with 58V.
摘要:针刺治疗冠心病和急性中风病人,“内关”和“足三里”为常用穴位,经针刺治疗后心功能与微循环均得到改善,全血粘度与血浆粘度明显降低。针刺“足三里”穴治疗高脂血症有明显疗效,使胆固醇、甘油三The effect of electroacupuncture "ZUSANLI" and "NEIGUAN" points on membrane fluidity of red cells in rabbits, has been determined by fluorescence polarization method. Results showed that the membrane fluidity of red cells was developed evidently after electroacupunctured "NEIGUAN",but "ZUSANLI" had no effect on it, and it was significantly developed by electroacupuncture "ZUSANLI" and "NEIGUAN" points at the same time.
摘要:流行性出血热(EHF)的基本病理改变为广泛性小血管损害。血浆血栓素(TXA_2)和前列环素(PGI_2)是体内调节血小板-血管壁功能的重要因子。因此,这两种血管活性物质与EHF的发展和转归有其重要的内在联系。我院周楣声等应用中医灸法治疗In this paper, the model of rat infected EHFV was made and the influence of moxibustion on TXA_2 and PGI_2 in its plasma was observed. The results show that the content of TXA_2 increased and PGI_2 decreased in rat significantly after the abdominal inoculation of EHFV, and the content of TXA_2 decreased and PGI_2 increased markedly in rat infected EHFV to normal level after treatment with moxibustion, suggesting that the regulative function of moxibustion on TXA_2 and PGI_2 is one of its nerve-emdocrine-immune regula tions to the body, and there is an important significance This study provides an impostant reference for mechanism exploration of moxibustion preventing and treating EHF.
摘要:针刺镇痛的研究中,外周神经中哪一类纤维参与针刺镇痛效应一直是个争论的问题。候宗濂等认为C纤维传入是主要的,张香桐等提出外周神经中Ⅱ、Ⅲ类纤维是重要的,吕国蔚等则看到主要是Aβr类Experiments were carried out in rats anaesthetized with urethane (1g/kg). Late discharges of spinal dorsal horn neurons were recorded as noxious responses to strong stimulation of right tibial nerve (a train of 3 pulses, 25V, 1ms). Left St.36 and Sp.6 were stimulated electrically (100Hz, 3V or 6V, 1ms). Left sciatic nerve was exposed and soaked for 15 min in 1.5% capsaicin or vehicle 24 hours before experiment. Left femoral nerve was sectioned. Spinal cord was transected at T4 level in spinal rat. Intact rats. 1) EA group (N=12). Sciatic nerve was not treated. Late dischages of neuron were reduced to 44.6±18.3% of control (P<0.02) by EA(3v). 2) Capsaicin group (N=15). Late discharges were reduced to 89.8±6.0% of control (P>0.05) by EA(3V). 3) Vehicle group (N=10). Late discharges were reduced to 51.2±15.6% of control (P<0.05) by EA(3V). 4) There was a statistical difference (P<0.05) between capsicin and vehicle group. Spinal rats: 1) EA group. Late discharges of neuron were reduced to 67.6±10.3% of control (P<0.02) in 11 neurons by EA(3V), 72.7+8.8% (P<0.01) in 9 neurons by EA(6V). 2) Capsaicin group. Late discharges were 110±21.0% of control (P>0,05) in 13 neurons after termination of EA (3V), 90.7±12.9% (P>0.05) in 10 neurons after EA (6V). 3) Vehicle group. Late discharges were reduced to 67.7±8.1% of control (P<0.02) in 12 neurons by EA (3V), 68.1±5.0% (P<0.01) in 10 neurons by EA (6V). 4) There was a statistical difference (P<0.05) between capsaicin and vehicle group for 3V, no statistical difference (P>0.05) for 6V. C fiber of periphral nerve is main component involved in effect of EA analgesia in intact rats. Both A and C fiber of periphral nerve involved in effect of EA analgesia in spinal rats.
摘要:氦氖(He-Ne)激光穴位照射,具有镇痛、消炎、提高机体免疫机能、促进血中免疫球蛋白(IgG、IgA、IgM·)浓度升高,并增强细胞免疫机能。但He-Ne激光穴位针刺对机体免疫器官的作用,特别对于产生免疫机能的T、B淋巴细胞及抗原提呈细The lymphocytes and antigen presenting cells in lymph node of rats stimulated by He-Ne laser acupumture were observed by using TEM and SEM to investigate the ultrastructural changes of them. There were numerous actived T-cells which showed deeply indented nucleus, abundant small void mitochondria and fres ribosomes in the paracortex area. The B-cells were gradually differentiated into large lymphocytes, immature and mature plasmatic cells which with a lot of rough endoplasmic reticulum. They were prominently increased in the pulp area. The macrophages had short processes with numerous folds and microvilli and tended to neighboring lymphocytes. The nucleus pores were increased. There were a lot of pinocytosomes, phagosomes, lysosomcs in various size of macrophages. The bundles (5-6nm in diameter) of microfilaments of the macrophages were extended from the cytoplasm to the processes. The interdigitating cells which contained the characterized single layer of rER, numerous polysomes, mitochondria and well-developed Golgi-complex were closed to macrophages and lymphocytes. In conclusion the activities of the cellular immunity and humoral immutVity were enhanced by laser.
摘要:根据针刺手法“补则针下热,泻则针下凉”临床效应,近代学者认为,这种“热”,“凉”效应与局部血管舒缩运动有密切关系。研究者们曾先后采用皮温、血管容积脉搏波、微循环技术、红外线成像等有关指标In order to prove the specific property between the tonifying manipulation (TM) and reducing manipulation (RM), and search further for the key in improving the effect of manipulation, the influence of to on two different manipulations, the plethysmogram in the same body-condition (kidney-deficiency) One was TM with slow-trusting and quick lefting; the other was RM with the converse movement. Randomized block design, cross-over design and single-blind design were used. The result showed that the TM increased the amplitule of the plethysmogram obviously, wheras the RM had no significant influence on it. There was significant difference not only between the TM group and the contral group, but also between the TM group and RM group (P<0.01 or P<0.05). The difference between the RM group and the contral group was not significant (P>0.05). Meanwhile, it was recommanded that "mind concentration" on acupuncture manipulation is important. In conclusion, the experiment demonstrated the experimental evidence for explanning the machanism of TM and RM. It was suggested that acupun cture manipulanions are worthy to pay attention so as to improve clinical effect.
摘要:以往的实验已经证实了放射性核素是客观显示经络循行的一种好方法,并对其所显示的循经迁移轨迹的特性进行了一系列的研究。为进一步阐明放射性核素所显示的循经迁移轨迹的本质,我们将这种方法移植到动物体上先后在狗、猫、兔等动物身上重复了上述实验。在与人种系发生关系极为密切的灵长类动物的身上也重复了上述实验结果,在On the basis of researchs in human bady, further experiment in monkeys was conducted and the result has demonstrated that: 1. There was a close and complicated relation between the migration of 99mTc injected into acupoints and the blood circulation. 2. The location of the migration was mainly in the subcutaneous lay. 3. There were no direct relationship between the migration and periphery nerve system.