摘要:本实验选用家猫 ,在红外热像仪监视下 ,选取针刺后皮层温度较高和较低部位的组织进行分析 ,用酶学方法测定Na+,K+ ATP酶活力 ,放射免疫方法 (RIA)测定P物质 (SP)含量。实验结果如下 :针刺后 ,皮层温度较高部位 (3 4.9± 1 .1 3℃ )的Na+,K+ ATP酶活力与皮层温度较低部位 (3 3 .3 8± 1 .0 3℃ )比较 ,有明显升高 (P <0 .0 1 ) ;皮层温度较高部位SP含量亦较温度低部位显著升高 (P <0 .0 2 )。结果提示 ,针刺后大脑皮层某些区域温度的高低可能与中枢Na+,K+ ATP酶活力增强有关 ,同时和中枢SP的功能活动有关Domestic cats were used in this experiment. After acupuncture, the higher temperature area and lower temperature area of the cerebral cortex were dissected respectively under the monitoring of the infra red thermography. The activities of the Na +,K + ATPase in the cerebral cortex were determined with enzyme method and the contents of SP (substance P) were determined with radioimmunoassay. The results show that the activities of Na +, K + ATPase in the higher temperature area (34.90±1.13 ℃) is higher than that in the lower temperature area (33.38±1.03 ℃), P<0.01;the contents of SP in the higher temperature area is also higher than that in the lower temperature area (P<0.02) after acupuncture. It suggests that the change of temperature caused by acupuncture at some parts of the cerebral cortex is related to the activities of Na + ,K + ATPase and the contents of SP in the central nervous system of the cat.
摘要:采用暂时性脑缺血再灌注模型和组织化学、TUNEL细胞标记等实验技术 ,观察神经细胞死亡和一氧化氮合酶 (NitricOxideSynthase,NOS)水平的变化。实验结果表明 ,暂时性脑缺血再灌注时脑内NOS表达显著增加 ,且引起延迟性细胞死亡 ,包括坏死与凋亡。电针可明显减少神经细胞坏死 ,在存活细胞增多的基础上 ,TUNEL阳性细胞的比例有所增加 ;与此同时 ,电针亦使脑内NOS表达显著减弱。提示电针具有神经保护作用 ,且可能与其下调NOS、减少NO的过量生成有关Transient cerebral ischemia/reperfusion model was adopted,and the method of TUNEL(TdT mediated dUTP Nick End Labeling),immunohistochemistry are used to observe the neuronal death and change of nitric oxide synthase level. The results showed that transient cerebral ischemia/reperfusion evidently increased the expression of NOS, as well as induced the delayed neuronal death, which included both necrosis and apoptosis. EA evidently decreased the expression of NOS and necrotic neuron loss, increased the percent of apoptotic cells and survived cells. It sugested that EA protective effect may be related with reducing the increased NOS and decreasing the excess production of NO.
摘要:用玻璃微电极记录方法在清醒麻痹的家兔上探讨扣带回前部 (ACC)痛单位的电活动及其对外周伤害性刺激反应的可能机制。结果发现 ,ACC的痛单位占引导神经元数的 9.5 % ;其中 ,痛兴奋神经元 1 2 2个 ,占痛单位的 81 .3 % ;痛抑制神经元 2 8个 ,占痛单位的 1 8.7%。除对照组外 ,大多数痛单位对伤害性刺激的痛反应均明显受受体拮抗剂或激动剂如酚妥拉明、氟哌啶醇、阿托品、地西泮、赛庚啶等的影响。提示 :扣带回前部参与痛信息的管理 ;外周神经及中枢许多相关核团可能通过去甲肾上腺素、多巴胺、乙酰胆碱、γ 氨基丁酸、5 羟色胺或组胺等多种神经递质及相关受体共同调制ACC痛单位的电活动This study using the micro electrode recording method investigated the electric activity of the pain units of the anterior cingulate cortex (ACC) and its response mechanism to peripheral noxious stimulation in conscious paralytic rabbits. The results showed∶ the pain units of ACC amounted to 9.5% of the neurons recorded; among them, PEN was 122 and PIN, 28. The pain response of the most pain units to peripheral noxious stimulation was affected apparently by phentolamine, haloperidol, atropine, diazepam, cyproheptadine. The data demonstrate that ACC participates in the management of pain; peripheral nerve and many central nuclei may modulate the electric activity of the pain units of the ACC through norepinephrine, dopamine, acetylcholine, γ amino butyric acid, 5 hydroxytryptamine or histamine and its receptors.
摘要:本实验采用高脂饲料喂养SD雄性大鼠制造肥胖动物模型 ,并观察针刺对其摄食、体重、脂肪、杏仁核组织单胺类神经介质含量的影响。结果显示 ,针刺可以明显降低肥胖大鼠摄食量、体重、Lee’s指数、脂肪量 ,说明针刺具有减肥作用。本文可见 ,肥胖大鼠杏仁核酪氨酸 (Tyr)、多巴胺 (DA)和去甲肾上腺素 (NA)的水平明显低于正常 ,而 5 羟色胺 (5 HT)和 5 HT/ 5 羟吲哚乙酸 (5 HIAA)的水平明显高于正常。针刺治疗取得良好减肥效应 ,同时肥胖大鼠杏仁核Tyr、DA水平明显回升 ,5 HT和 5 HT/ 5 HIAA水平明显回降。提示 ,针刺对肥胖大鼠杏仁核的良性作用可能是其实现减肥效应的中枢作用机制之一In this experiment the amount of food and water taken in,body weight,body fat,and monoamine content of amygdala in obesity model of male SD rats obtained by high fat diet were observed before and after acupuncture. The results showed that the contents of tyrosine (Tyr),dopamin(DA)and noradrenaline(NA)were lower in control group than those of normal,but the contents of serotonin(5 HT)and 5 HT/5 hydroxyindoleacetic acid(5 HIAA) were higher than those of normal.After acupuncture treatment,the weight loss was achieved while the level of Tyr and DA increased, the level of 5 HT and 5 HT/5 HIAA decreased in amygdala of obese rats. It suggests that a good regulative effect of acupuncture on amygdala of obese rat is an important link in the antibesity.
摘要:本实验建立佐剂型关节炎模型 ,观察挑筋疗法的抗炎免疫作用 ,且运用末端标记法检测挑筋法对大鼠胸腺细胞凋亡的影响。结果显示 ,挑筋疗法能减轻局部炎症反应 ,消退足部肿胀 ,使跖围减小 ,胸腺重量及厚度较模型组有显著下降。挑筋组和药物组 (地塞米松 )均出现细胞凋亡 ,凋亡率分别为 40 86%± 3 3 5 %、3 8 5 1 %± 3 5 9% ,两者无明显差异 (P >0 .1 )。实验表明 ,挑筋法可诱导大鼠胸腺细胞凋亡In Freund's arthritis animal model, the effect of fibrous tissue broken pricking on thymic apoptosis was studied in the mouse. The result is:fibrous tissue broken pricking can relieve the local inflammatory and antiontic matatarsal joint. In medicine group and pricking group, we found cell death in mouse thymus.It proves that fibrous tissue broken pricking can induce apoptosis in mouse thymus.
摘要:对家兔做胆囊造影后 ,通过X线检查胆囊运动的实时变化来了解胆囊运动的功能状态。实验性胆囊炎家兔的胆囊较正常家兔有所扩大 ,运动障碍明显。耳 体穴电针对轻症胆囊炎家兔的胆囊能加强其收缩运动 ,在 1小时后就见胆囊明显缩小 ,其效果超过胆囊收缩素 (CCK) ,但对重症胆囊炎者无明显作用。说明耳 体穴电针不仅可以作为治疗胆囊炎、改善胆囊功能障碍的手段 ,而且可作为一种激发措施来判断胆囊运动功能障碍的程度The timely change of gallbladder motility in rabbits was observed under X ray examination after gallbladderography for understanding gallbladder function. The gallbladder of rabbit with experimental cholecystitis has an enlargement to more extent than the healthy rabbit's gallbladder and an obvious dysmotility occurred. The contractive motility of gallbladder in rabbit with a slight cholecystitis might be strengthened by electroacupuncture at auriculo and body acupoints. After an hour of electro acupuncture, the gallbladder was reduced obviously. The effect of electroacupuncture surpass cholecystokinin (CCK). But the severe cholecystitis failed to respond to electroacupuncture. The above studies show that electroacupuncture at auriculo and body acupoints not only is a method to treat cholecystitis and alleriate gallbladder dysmotility, but also may be regarded as a provocation test to decide the degree of gallbladder dysmotility.
摘要:本实验采用链脲佐菌素 (STZ)诱发大鼠实验性糖尿病 ,以坐骨神经中cAMP、cGMP作为检测手段 ,观察了针刺对糖尿病大鼠的治疗作用。结果显示 ,糖尿病大鼠 (DM)坐骨神经中cAMP、cGMP含量明显低于正常大鼠 (ND) (P <0 .0 1 ) ,而给予STZ糖尿病大鼠针刺治疗 4个月后 ,针刺治疗组 (Ac)坐骨神经中cAMP、cGMP含量与造模组比较有明显差异 (P <0 .0 1 )。提示针刺可防止糖尿病大鼠坐骨神经中cAMP、cGMP含量的下降 ,对神经轴突的再生与修复有促进作用Intra peritoneal administration of streptozotoxin(STZ) produced diabetes in the Wistar rats. One week after STZ injection the animals were considered to be diabetic rats if the contents of their blood glucose were higher than 16.7 mmol/L.Then the diabetic rats were randomly divided into two groups,diabetic group (DM) without any treatment and acupuncture group (Ac) treated with acupuncture. Four months after treatment the animals were sacrificed and their sciatic nerves were taken out for cAMP and cGMP detection. The concentrations of cAMP and cGMP in the sciatic nerve of rats in DM group were significantly lower than those in age matched non diabetic group (ND) and Ac group (P<0.01, respectively). There was no significant difference between ND and Ac group. The results suggested that acupuncture therapy might prevent the decrease of the contents of cAMP and cGMP in the diabetic sciatic nerve.
摘要:运用艾灸血清和小鼠胸腺细胞瘤 (EL 4)进行细胞培养 ,观察艾灸血清在体外对肿瘤细胞形态、细胞周期及DNA断点的影响。结果发现艾灸血清能诱导肿瘤细胞凋亡 ,引起肿瘤细胞在形态上发生较典型的凋亡改变 ,影响肿瘤细胞由S期向G2 M期的转变过程 ,提高肿瘤细胞DNA断点标记阳性率。说明艾灸血清中可能含有某些已知或未知的能对肿瘤细胞生长产生抑制的成份By culture EL 4 tumor cells with Murine Moxibustion Serum(MS),influence of MS to cell structure, cell cycle and breaking point of DNA was studied. The results proved that MS could induce apoptosis of tumor cells, result in typical apoptosis changes in vitro,influence transforming process of tumor cells from phase S to phase G 2M and enhance the positive marking percentage of breaking point of DNA. The conclusion is that MS may contain certian known or unknown ingredients which inhabit the growth of tumor cells.
摘要:用Northern印迹分析艾灸结合免疫调节剂抑制肿瘤细胞增殖的机制。结果显示 ,艾灸及其结合免疫调节剂可明显降低c erbB 2mRNA的表达水平。表明艾灸及其结合免疫调节剂抑制肿瘤细胞增殖 ,可能与下调c erbB 2基因转录水平 ,进而降低基因表达水平有关Molecular biological mechanism of the action of moxibustion combined with immunomodulator was investigated, using the mice which were subcutaneously transplanted with H 22 (Haptoma) cell line as a solid carcinoma model. The result showed that c erbB 2 mRNA expression level was obviously reduced in tumor tissue in moxibustion group and group moxibustion and immunomodulator combination(group M+IM),as compared with tumor control group. The result implied that inhibition of tumor cell proliferation by moxibustion and immunomodulator combination was related to the reduction of c erbB 2 mRNA through decreasing rates of c erbB 2 gene transcription.
摘要:采用隔药饼灸结合1 37CSγ射线放疗对实体瘤 (HAC)小鼠进行研究 ,不同时间肿瘤体积及瘤重抑制率表明 ,艾灸能增强对乏氧肿瘤细胞的杀伤作用。小鼠荷瘤后NK、LAK、IL 6活性及IL 2含量均明显降低 ,放疗后除NK活性外 ,均进一步降低 ,经艾灸治疗可以明显提高NK、LAK及IL 6活性和IL 2含量 ,其中LAK活性达到正常水平。提示隔药饼灸可促进肿瘤放疗后免疫系统损伤的恢复This article studies the effect of indirect moxibustion on HAC tumor bearing mice treated with radiotherapy. The results of hypoxic tumor regeneration after irradiation showed that combination of the indirect moxibustion and radiotherapy had the coordinating efficiency of antitumor effect. The NK activity, LAK activity, IL 2 content and IL 6 activity of tumor bearing mice were all significantly depressed. All the above parameters were further depressed after irradiation except for NK activity. Indirect moxibustion could promote all the parameters in different degrees, and LAK activity reached the normal level. The results showed that indirect moxibustion's influence on IL 6 activity and IL 2 content can promote NK activity, LAK activity and recover immunological function.
摘要:采用荧光组织化学方法 ,在同一条件下 ,用乙醛酸 (SPG)荧光染色和吖啶橙 (AO)荧光染色 ,在荧光显微镜下观察 ,电针后肥大细胞 (MC)释放的物质是以胺类为主 ,能扩张毛细血管及静脉 ,通过神经体液对机体进行一系列的调节 ,而显示针刺效应The authors observed the localization and defection of released materials by MC in deep aponeurosis under the fluorescent microscope by using the fluorescent chemical method of SPG and AO in same condition. The results showed that:(1)Released materials by MC are mainly histamine after the electric needling in “Zusanli”. (2)These materials can dilate capillaries and veins,which can regulate the organ function by nervous body fluid. These results suggest that the acupuncture effect can be manifested.
摘要:本文通过对 2例病毒性脑炎皮层盲患儿的治疗 ,观察了脑干视觉诱发电位 (VEP)和脑地形图在头针脑炎皮层盲中的动态变化。以毫针沿皮刺头穴的双侧足运感区、视区及风池穴并连接电针仪 ,结果显示头针有促进病理性脑电波恢复的作用 ,从而降低了脑炎导致失明的致残率Through the treatment on 2 infantile cases with virus cerebritis cortical blind, dynamic changes of VEP and cerebral cortical blind under the treatment of scalp acupuncture were observed. The treatment was to puncture head points on both sides beneath the skin, the remote sense zones of foot, vision and Fengchi point connected with G6805 II type of electric acupuncture device. The result showed that scalp puncture could improve the pathological recovery of the cerebral wave and reduce the rate of disability caused by cerebritis.
摘要:自 1 995年 1月~ 1 997年 6月我院针药麻组应用手法针刺穴位加针刺增效药静脉复合麻醉施行肺切除术 ,并进行临床研究 40例 ,使病人在浅麻醉状态下持续手法捻针完成肺切除手术 ,同时设全麻对照组 40例。结果针药麻组静脉复合液用量与全麻对照组比较 ,减少用量 45 % ;针麻效果优良率 78% ,成功率 1 0 0 %。该方法不同于以往辅助用药 ,一改过去的被动用药为主动用药From January, 1995 to June, 1997, the acupuncture and drug anesthesia group applied manipulation of acupuncture and compound vein anesthesia for reinforcing the effect of acupuncture during the operation of lung removal. 40 cases were studied and 40 cases treated with general anesthesia were kept in control group for comparison. As a result, the acupuncture and anesthesia group used less compound drug liquid than the control group by 45%, and the fine rate of acupuncture anesthesia was 78%, the successful rate was 100%. This method is different from the auxiliary medicine for it is an improvement from passive drug using to active drug using.
摘要:本文探讨了胃肠经特定穴位对非溃疡性消化不良的影响。将 65例非溃疡性消化不良患者随机分为四型 ,1 6例溃疡样消化不良 ,1 3例反流样消化不良 ,2 7例动力紊乱样消化不良 ,9例非特异性消化不良。对四型之间疗效及治疗前后血浆MTL进行比较 ,结果 :溃疡样、反流样、非特异性消化不良之间疗效无差异 (P >0 0 5 ) ,但动力紊乱样消化不良疗效与其它型比较有显著性差异 (P <0 0 5 ) ;治疗前后血浆MTL比较 ,显著性差异 (P <0 0 5 )。提示本法对非溃疡性消化不良疗效满意 ,且能改善患者的血浆胃动素MTL浓度Purpose: To study the effect of acupuncture treatment on special points on non ulcerative indigestion. Method: 65 Cases of non ulcerative indigestion were divided into four types. 16 cases had ulcer like indigestion, 13 cases had reflux indigestion, 27 cases had indigestion due to dynamic disorder and 9 cases had non specific indigestion. The blood plasma MTL (pol/L) of the four types was compared before and after treatment. Result: The effects of the ulcer like type, reflux type and non specific type had no difference (P>0.05), while the dynamic disorder group had difference (P<0.05). Conclusion: This method had satisfactory effect on the treatment of non ulcerative indigestion and it could improve the concentration of MTL.
摘要:本文观察了采用阳陵泉穴针刺方法对胆绞痛患者首诊的疗效。用毫针从阳陵泉穴向窝方向刺入 1 5寸 ,采用捻转泻法 ,得气后留针 3~ 3 0分钟 ,每隔 3分钟行针 1次。结果 79例首诊患者中显效 67例 ,显效率为 84 81 % ,平均显效时间为 5 5 5分钟 ;总有效率为 93 67% ,平均有效时间为 7 71分钟。提示用泻法针刺阳陵泉穴能迅速、有效地缓解胆绞痛The essay is about the observation on the treatment of acupuncture on Yanglingquan point for the first visiting patient with colic pain of bile cyst. The treatment was to puncture 1.5 cun into the point Yanglingquan to the direction of popliteal fossa by twisting, turning for reinforcing and purging. Maintain the needle for 3~30 minutes when there was the sense of needling. Manipulating the needle every 3 minutes. As a result, 79 cases showed notable effect, 84.81%, the total time for effect response was 5.55 minutes, and the total efficiency was 93.67%, the average time for effect showing was 7.71 minutes. This indicated that the purging method of acupuncture on Yanglingquan point could quickly and effectively relieve the colic pain of bile cyst.
摘要:对 90例具有不同程度疼痛症状的癌症病人分别进行了针刺镇痛、中药内服外敷镇痛、WHO药物 3级止痛阶梯和针药结合治疗。结果表明 ,针刺组和中药组镇痛效果优于西药组 ,有显著性差异 (P <0 0 5 ) ,针药组与西药组相比有非常显著性差异 (P <0 0 0 1 )。说明针刺和中药镇痛优于西药止痛 ,针刺和中药结合治疗癌症疼痛有较高的临床应用价值cancer patients with different degree pain were observed in the study.They were divided into 4 groups:acupuncture group, Chinese Herbs group(combination of oral medication and Chinese herb iontophoresis treatment) ,western medicine group and Chinese herbs and acupuncture group. The results showed that the analgesic effect of acupuncture group and Chinese herbs group was better than that of the western medicine group.The Chinese herbs and acupuncture group had a very significant difference from the western group.Therefore,we can conclude that the acupuncture and Chinese herbs' analgenic effect on cancer patients has a high value in clinical treatment.
摘要:本文以针罐灸并用治疗 92例带状疱疹患者 ,均获痊愈 ,治疗最少 2次 ,最多 6次 ,平均治愈次数为 3次。临床实践证明 ,三法齐用 ,止痛快 ,奏效迅速cases under the study were cured and the treatment was 2~6 times, the average treatment was 3.1 times. The combined treatment with acupuncture, moxibustion and cupping was effective and quick in pain killing.
摘要:本文探讨了《内经》中针刺获效的影响因素。主要从以下几方面进行论述 :①体质因素 ;②神气因素 ;③年龄与形体因素 ;④时间因素 ;⑤补泻因素 ;⑥针刺浅深因素 ;⑦针具因素。提示以上各因素均可直接或间接地影响针刺获效和得气 ,故临床上必须依据《内经》针刺原理指导其针刺的运用 ,以提高临床效果The essay discusses on the affecting factors of acupuncture in the classic of Internal Medicine in the following aspects: (1) Constitution factor, (2) Vitality and Qi, (3) Age and shape of the physical body, (4) Time factors, (5) Reinforcing and purging methods, (6) The depth of acupuncture, (7) Needles. The above factors can directly or indirectly affect the effect of acupuncture and the obtaining of sense of needling. Therefore, in clinic the application of acupuncture should be based on the principles in the classic Internal Medicine so as to obtain better clinical effects.
摘要:本文分析了当前在经络实质研究中存在的问题以及经络实质至今未能有统一认识的主要原因 ,认为把经络实质问题可以简化为经穴 脏腑相关问题 ,根据人体神经系统的特点 ,把经穴 脏腑相关问题进一步分解成和脊神经有关的以及和脑神经有关的两个问题进行研究 ,使经络实质研究问题简化 ,只有这样 ,才能使研究工作较容易进行 ,并阐明了对待古典经络理论的态度We analyzed the problems in the studying about the nature of meridians and found the major reasons caused the divergent cognition about it. We considered that the studying about the nature of meridians can be simplified as about the correlation between acupoints and viscera, and the latter can be resolved into two problems related to spinal nerve and cranial nerve based on the neural specialty of body. We believed that the study will be more easier through the simplification and analysis.
摘要:本文就针刺治疗急性缺血性中风中有关针刺对脑缺血组织损害的修复作用及其调节机制的实验研究的最新进展作一综述 ,并对研究结果及意义进行了一定的探讨The study summarized the recent advances of experimental research in the treatment of ischemic cerebral apoplexy with acupuncture; the advances of improving the injury of ischemic cerebral apoplexy and the regulation mechanism of acupuncture.
摘要:经过对 1 981~ 1 998年公开发表在 2 0 0余种刊物有关针灸治疗支气管哮喘的临床观察和实验报道的论文 2 40余篇进行分析 ,拟从选穴、用穴规律角度出发 ,探讨针灸治疗支气管哮喘的规律。结果显示 ,治疗原则为“急则治其标 ,缓则治其本” ,选用经络多取任脉、督脉、膀胱经、肺经、胃经、大肠经 ,取穴多在胸背部 ,十二经脉中均有取穴 ,治疗以灸法、穴位敷贴、刺络拔罐等为主要手段After analyzing about 240 clinical and experimental reports of acupuncture therapy for asthma in more than 200 publications from 1981~1998,we concluded the rule of acupuncture therapy for asthma from the angle of methods of selecting and using acupoints. The results are: (1) the principle of therapy is treating tip in acute condition and treating root in chronic condition;(2) the mainly selected meridians are Ren channel, Du channel, Bladder channel, Lung channel, Stomach channel and Large intestine channel; (3) the mainly used acupoints are distributed in chest and back; (4) the main methods are moxibustion, acupoints attachment, stinging and cupping.