最新刊期
2001年第2期
- 摘要：目的 :本研究主要观察电针和兴奋尾核对苍白球神经元自发放电的影响 ,为进一步研究苍白球与针刺镇痛和兴奋尾核镇痛的关系及其机理提供某些实验资料。方法 :实验在Wistar大鼠和家猫上进行。采用玻璃微电极细胞外记录的方法记录苍白球神经元的自发放电 ,观察电针“阳陵泉”和“环跳”穴 (强度为 2mA ,频率为 2～ 10 0Hz的疏密起伏波 ,时间为 5min)及兴奋尾核头部 (微量注射 0 .5M谷氨酸 3μL)对自发放电的影响。结果 :电针可抑制苍白球外侧部神经元的自发放电 ,对内侧部自发放电略有兴奋作用 ;兴奋尾核也可抑制苍白球外侧部神经元的自发放电 ,而对内侧部自发放电影响不大。结论 :电针对苍白球神经元自发放电有一定的影响 ,可能与兴奋尾核有关Objective: To observe whether spontaneous discharges of neurons in the globus pallidus could be influenced by electroacupuncture or excitation of the caudate nucleus, so as to investigate further if the globus pallidus was involved in acupuncture and exciting caudate nucleus induced analgesia. Methods: The present experiments were performed on cats and Wistar rats. Spontaneous discharges of neurons in the globus pallidus were recorded by glass microelectrodes extracellularly. All animals received electroacupuncture of “Yanglingquan" (GB 34) and “Huantiao" (GB 30) points (3 mA, 2～100 Hz dense sparse waves, 5 min). Excitation of neurons in the caudate nucleus was achieved through microinjection of glutamate (0.5 M, 3 μL) into the nucleus. Results: We found that the spontaneous discharges of the neurons recorded in the lateral region of the globus pallidus could be inhibited by electroacupuncture , and those of the neurons recorded in the medial region of the globus pallidus could slightly be enhanced by electroacupuncture. Similar results were acquired through the excitation of the caudate nucleus. Conclusion: Electroacupuncture can influence spontaneous discharges of neurons in the globus pallidus, which may be related to the excitation of the caudate nucleus.关键词：苍白球;自发放电;尾核;电针832|0|2更新时间：2023-08-11
- 摘要：低频 (2Hz)和高频 (1 2 8Hz)电针大鼠“合谷”穴 ,采用还原型尼克酰胺嘌呤二核苷酸脱氢酶 (NADPH d)法 ,显示不同频率电针对尾壳核头部各区一氧化氮合酶 (NOS)表达的影响。结果表明 ,低频和高频电针均可使尾壳核头部NOS表达增强 ,高频电针上调尾壳核头部NOS表达更为显著Objective: To study the effect of electroacupuncture (EA) of “Hegu” (LI 4) at different frequencies on nitric oxide synthase (NOS) expression in the nucleus caudatus and putamen in the rat. Methods: 18 SD rats were randomly divided into control group (n=6), 2 Hz EA group (n=6) and 128 Hz EA group (n=6). Unilateral “Hegu” (LI 4) was punctured and stimulated with electrical pulses (3 V, duration of 1 ms, 2 Hz and 128 Hz) for 30 min. Changes of NOS expression were displayed by using nicotinamide adenosine dinucleotide phosphate (NADPH d) method and observed under microscope. Results: In comparison with control group, the number of NOS positive neuron in the dorsal lateral, ventral lateral and dorsal medial regions of the nucleus caudatus and putamen were increased significantly in both 2 Hz EA group and 128 Hz EA group (P<0.05). The fact suggests that nitric oxide of this nucleus may participate in acupuncture analgesia. Conclusion: Both 2 Hz and 128 Hz EA of “Hegu” (LI 4) can induce up regulation of NOS expression in nucleus caudatus and putamen in the rat.关键词：“合谷”穴;电针;一氧化氮合酶;尾壳核1083|0|3更新时间：2023-08-11
- 摘要：应用酶联免疫吸附实验 (ELISA)法测定缺血大鼠电针后脑内脑源性神经营养因子(BDNF)含量的变化。实验分正常组、缺血再灌组和缺血再灌 +电针组 ,大脑中动脉阻塞 (MCAo)致局灶缺血 90min ,缺血后立即给予电针 ,取“水沟”和“百会”穴 ,强度 3.5mA ,频率 1 0 0Hz,时间1hr。再灌 8hr后取缺血侧皮质 ,ELISA法测定。结果正常组、缺血再灌组和缺血再灌 +电针组脑内BDNF含量分别为 1 4.2 0± 3.1 4、1 8.75± 2 .6 3和 2 3.75± 3.0 2 (ng/ g) ,缺血 +电针组BDNF含量多于缺血组 (P <0 .0 5 )。表明电针能提高缺血后脑内BDNF的合成或释放Objective: To observe the effect of electroacupuncture (EA) on brain derived neurotrophic factor(BDNF) content of the cerebral cortex in rats with cerebral ischemia. Methods: 18 Wistar rats were randomly and evenly divided into normal control, ischemia and EA+ischemia groups. The middle cerebral artery was occluded for 90 min to induce focal ischemia. After cerebral ischemia, EA (3.5 mA, 100 Hz) was applied to “Shuigou” (GV 26) and “Baihui” (GV 20) for one hour. After 8 hr reperfusion, the ischemic cortex was sampled and the BDNF content determined with enzyme linked immunosorbent assay (ELISA). Results: It was found that BDNF contents in the infarction region of the cerebral cortex in control, EA and EA ischemia groups were 14.20±3.14, 18.75±2.63 and 23.75 ±3.02 ng/g respectively. Conclusion: EA could increase the synthesis and release of BDNF in the brain after cerebral ischemia and reperfusion.关键词：脑缺血;电针;脑源性神经营养因子1031|0|5更新时间：2023-08-11
- 摘要：本文对即早表达基因c fos在电针抗局灶性脑缺血损伤中的作用进行了探讨。结果表明 ,局灶性脑缺血可以引起c fos在缺血侧皮层的大量表达 ,电针能部分抑制这种表达 ,使局灶性脑缺血动物模型的脑梗塞灶减小。但c fos反义寡核苷酸脑内注射可完全阻断c fos表达 ,导致脑梗塞灶体积明显增大 ;同时也取消了电针的抗脑缺血损伤作用。提示脑内c fos适度的表达 ,可能在脑缺血损伤中有一定的保护作用 ;电针可能通过部分抑制脑内c fos的过度表达 ,发挥其抗局灶性脑缺血损伤的作用Objective: To explore the role of c fos expression (one early gene) in electroacupuncture (EA) resisting cerebral focal ischemic injury in the rat. Methods: ① 36 SD rats were randomly divided into ischemia group and ischemia + EA group each of that was further divided into 6 subgroups according to the duration of reperfusion (1 hr, 2 hr, 4 hr,8 hr,12 hr and 24 hr) after 90 min of cerebral ischemia. “Baihui" (GV 20) and “Shuigou" (GV 26) were stimulated with EA (3.5 mA, 100 Hz, 60 min). ② 24 SD rats were randomly and evenly divided into antisense, sense, mismatch and normal saline (NS) groups. C fos antisense (3 nmol/2 μL), sense (2 μL), mismatch (2 μL) and NS(2 μL) were separately injected into the ischemic central area of the rat brain (APO, L: 3.5 mm, H: 5.5 mm) in different groups. ③ The brain samples were stained with immunohistochemical (ABC) method and c fos positive neurons were calculated under microscope. Results: ① From the first hour on after cerebral ischemia and reperfusion, heavy expression of c fos appeared in the cortex of the ischemic side, it peaked at 4 hr and lowered from 8 hr on. Till 24 hr after ischemia, c fos expression quantity was similar on the ischemia and non ischemia sides. In acupuncture+ischemia group, c fos positive neuron count was significantly less than that of ischemia group. ② After microinjection of antisense in antisense group, fewer c fos positive neurons were seen around the ischemic region; while more c fos positive neurons found in sense group, mismatch group and NS group. The percentages of infarction volume/whole brain volume in antisense, sense, mismatch and NS were 12.69±6.67, 2.27±0.93, 2.69±1.39 and 2.51±1.77 respectively, that of antisense group was significantly larger than those of the rest 3 groups (P<0.05). Conclusion: Appropriate expression of c fos may have a certain protective action on cerebral ischemic injury; the inhibitory effect of EA on the superfluous expression of c fos in the brain may contribute to its resisting ischemic injury effect on cerebral neurons.关键词：局灶性脑缺血;电针;反义寡核苷酸;即早表达基因c-fos1093|0|4更新时间：2023-08-11
- 摘要：以热凝法阻断大鼠一侧大脑中动脉 (MCAo)造成局灶性脑缺血为实验模型 ,用原位末端标记 (TUNEL方法 )技术观察缺血区脑细胞凋亡的动态变化及针刺的干预作用。结果表明 ,MCAo后 1hr脑缺血区已出现细胞凋亡 ,2 4hr达到高峰。凋亡细胞主要局限于梗塞灶中心坏死区域的周边“半影区”。“半影区”的细胞凋亡可能对梗塞灶的扩大起着重要作用。“醒脑开窍”针法能使缺血区脑细胞凋亡明显减少 ,从而减小梗塞面积 ,起到脑保护作用Objective: To observe changes of apoptosis in the cerebral ischemic area and the intervention effect of acupuncture in the rat. Methods: 160 Wistar rats were randomly divided into pseudo operation group (n=10), model group (n=50), “Xingnao" needling (XN) group (n=50) and routine acupuncture (RA) group (n=50), the last 3 groups were further divided into 1 hr, 3 hr, 6 hr, 24 hr and 48 hr subgroup, with 10 cases in each subgroup. In XN group, “Neiguan" (PC 6) and “Shuigou" (GV 26) were punctured and stimulated with “Xingnao" needling, and in RA group, “Quchi" (LI 11) and “Zusanli” (ST 36) were punctured and stimulated with routine needling method. Cerebral ischemia model was produced by thermocagulation occlusion of unilateral middle cerebral artery. Apoptosis staining was performed with TUNEL technique. Results: Along with the increase of cerebral ischemic duration, the count of apoptotic neurons mainly in the “penumbra area" around the central necrosis region raised gradually and peaked at the 24 th hr after ischemia. After acupuncture treatment, the counts of apoptotic cells in cerebral cortex, striate body and pre optic hypothalamic area in all the subgroups of XN and most subgroups of RA group decreased significantly in comparison with model group (P< 0.05, 0.01). The effect of XN was superior to that of RA. Conclusion: “Xingnao Kaiqiao" needling method can significantly relieve apoptosis and reduce cerebral infarction area.关键词：“脑醒开窍”针刺法;大脑中动脉阻断(MCAo);细胞凋亡1572|0|6更新时间：2023-08-11
- 摘要：目的 :探讨耳针对血管性痴呆大鼠 (VD)学习记忆障碍的改善与bcl 2凋亡蛋白表达的关系。方法 :采用 4 血管阻断制备VD大鼠模型 ,针刺脑、肾耳穴后 ,作免疫组化、Nissl染色、行为学测试及图像分析。结果 :与正常对照比较 ,VD大鼠海马CA1 区神经元严重丢失 ,而bcl 2蛋白表达增加 ,大鼠学习记忆产生明显障碍 ;耳针治疗后海马CA1 区神经元丢失减轻 ,但bcl 2增加更为明显 ,此时大鼠学习记忆障碍得到明显改善。结论 :耳针改善VD大鼠学习记忆 ,可能是通过针刺调控细胞凋亡 ,达到对VD大鼠海马神经元的保护作用Objective: To study the relationship between auricular acupuncture improving dysmnesia and expression of bcl 2 protein in vascular dementia (VD) rats. Methods: 40 Wistar rats were randomly and evenly divided into control group, VD group, acupuncture+VD group and pseudo operation group. Rat VD model was established by using 4 vessel occlusion method. Otopoint “Nao” point and “Shen” (MA SC) were punctured, once daily continuously for 15 days. Rats' memory capability was tested with Y maze method and bcl 2 expression in the brain tissues displayed with immunohistochemical method and detected with MIAS 2000 Image Analyzer. Results: After auricular acupuncture treatment, the VD rats' memory was raised significantly in comparison with VD group (P<0.01). In control, VD and auricular acupuncture groups, bcl expression positive neurons in CA 1 region of the hippocampus were 14.31±4.87, 28.67±5.63 and 65.74±8.19 respectively, suggesting that the improvement of memory caused by acupuncture may be via up regulation of bcl 2 expression (an inhibitory gene of apoptosis) to lighten neuron lesion of hippocampus. Conclusion: Auricular acupuncture can raise the learning and memory capability, which may be realized by regulation of apoptosis and protecting hippocampal neurons.关键词：耳针;血管性痴呆;学习记忆;Bcl-2;海马1198|0|3更新时间：2023-08-11
- 摘要：为了探讨针灸对脑老化的作用机理 ,我们观察了针灸“百会”、“大椎”、“命门”穴对D 半乳糖诱导的亚急性衰老小鼠脑组织中一氧化氮 (NO)、丙二醛 (MDA)的含量和超氧化物歧化酶(SOD)的活性变化的影响。结果表明亚急性衰老小鼠脑组织中NO、MDA含量明显增高 ,SOD活性明显降低 ,针灸能使上述指标逆转。提示针灸“百会”、“命门”、“大椎”具有抗脑老化的作用 ,其作用机理可能与其能抑制自由基反应 ,提高抗氧化酶活性有关Objective: To explore the mechanism of acupuncture in anti aging effect. Methods: 30 mice were randomly and evenly divided into control, model and model+acupuncture groups. Sub acute aging model was established by subcutaneous injection of D galactose (0.5 mL/L). “Baihui” (GV 20) was punctured; “Dazhui” (GV 14) and “Mingmen” (GV 4) were moxibustioned for 2×3 cones. After homogenating the brain tissue, nitric oxide (NO) content was detected with spectrophotometry, malonyldialdehyde (MDA) content assayed with thiobarbituric acid (TBA) method and superoxide didismutase (SOD) activity determined with xanthine oxidimetry. Results: In the brain tissues of the aging mice (model group), NO and MDA contents increased remarkably while SOD activity was lowered apparently compared with those of control group (P<0.01). After acupuncture treatment, the increased contents of NO and MDA were lowered significantly while the decreased SOD activity raised considerably in comparison with those of model group (P<0.01) but had no marked difference with control group (P> 0.05). Conclusion: Acupuncture has an effect in resisting cerebral aging by way of inhibiting reactions of free radical and raising the activity of antioxidase.关键词：针灸;抗衰老;一氧化氮;丙二醛;超氧化物歧化酶1286|0|21更新时间：2023-08-11
- 摘要：在证明针刺对肾上腺切除 (ADX)和普通大鼠哮喘模型具有明显抗哮喘作用的基础上 ,进一步研究了ADX哮喘大鼠经针刺治疗后的血清 (针刺血清 )对上述两种大鼠哮喘模型的作用。结果表明 ,ADX哮喘大鼠经针刺治疗后的血清可明显降低ADX和普通哮喘大鼠的气道阻力与外周血嗜酸粒细胞计数 ,针刺血清注射与针刺治疗的作用之间无明显差别 ,提示针刺血清具有与针刺类似的抗哮喘作用。但针刺血清明显降低两种哮喘大鼠的肺顺应性 ,而针刺则增加其肺顺应性 ,这种作用方式上的一些差异有待进一步研究Objective: To investigate the effect of serum [derived from adrenalectomized (ADX) asthma rats who accepted acupuncture treatment, “acupuncture serum"] on asthma. Methods: SD rats were randomly divided into ADX + asthma group, asthma group, ADX + asthma + acupuncture group, asthma + acupuncture group, ADX + asthma +serum group, asthma + serum group. “Dazhui" (GV 14), bilateral “Feishu" (BL 13) and “Fengmen" (BL 12) were punctured and the needles were retained for 20 min, which were performed once every other day, 7 sessions altogether. Blood samples were taken to be isolated into serum that was then diluted and injected into the ADX asthma rats via external jugular vein before attack of asthma evoked by egg albumin. Blood eosinophil count was got under microscope after treatment with eosinophil staining solution. Results: In ADX asthma and asthma rats who did not accept acupuncture treatment, after injection of the “acupuncture serum”the airway resistance, pulmonary compliance and eosinophil count were lowered significantly (P<0.05). While after acupuncture treatment, the pulmonary compliance in asthma + acupuncture group increased significantly in comparison with asthma group(P<0.05). The reduction effects of “acupuncture serum" on airway resistance and eosinophil count were the same to those of acupuncture treatment. Conclusion: “Acupuncture serum” has the same anti asthma effects in ADX+asthma rats and asthma rats but their effects on pulmonary compliance are different.关键词：针刺血清;哮喘;肾上腺切除;肺功能;嗜酸粒细胞计数992|0|7更新时间：2023-08-11
- 摘要：本实验采用透射电镜技术对家兔急性心肌缺血后心肌缺血区肌原纤维、线粒体及血小板结构的变化及针刺的作用进行了观察。结果 :①缺血组肌原纤维的明暗带变浅或消失 ,Z带呈屈曲状 ;线粒体积聚、肿胀 ,有的线粒体嵴断裂和血小板脱颗粒发生。②电针“内关”穴后 ,心肌的横纹和肌原纤维的明暗带清晰可见 ,多数线粒体未见肿胀和积聚成堆现象发生 ,血小板也没有出现脱颗粒。结果提示 ,针刺“内关”穴可保护心肌细胞免于在急性心肌缺血期间受损Objective: To observe the protective effect of electroacupuncture (EA) of “Neiguan” (PC 6) on ischemic cardiac myofibrilla, mitochondria and blood platelets in the rabbit. Methods: 12 mongrel rabbits were randomly and evenly divided into control group, ischemia group and ischemia+EA group. Bilateral “Neiguan” (PC 6) were punctured and stimulated with electrical pulses (3 V, 40 Hz and intermittent waves) for 30 min. Myocardial ischemia was produced by occlusion of the left ventricular branch of the coronary artery. After sectioning, the myocardial samples taken from the ischemic marginal region were observed by using a transmission electron microscope. Results: ① In ischemia group, both the dark and light bands of some myofibrilla became lighter in color or disappeared; Z bands presented tortuous like changes; mitochondria got aggregate and swollen. Some mitochondrial cristae were broken and degranulation of platelets appeared; ② In EA+ischemia group, both the dark and light bands of the myofibrilla could be seen clearly, the Z band was normal, no swelling and no aggregation of majority of the mitochondria and no degranulation of platelets were found. Conclusion: EA of “Neiguan” (PC 6) can protect the ischemic cardiac myocytes from injury.关键词：心肌缺血;电针保护作用;透射电镜1192|0|5更新时间：2023-08-11
- 摘要：本实验采用高脂饲料喂养SD雄性大鼠制造肥胖动物模型 ,观察针刺对其体重、体脂和纹状体组织单胺类神经介质含量的影响。结果显示 ,肥胖大鼠纹状体酪氨酸 (Tyr)、多巴胺 (DA)、去甲肾上腺素 (NA)、色氨酸 (Trp)、N+,K+ ATP酶和Ca2 + ATP酶均明显低于正常水平 ,而 5 羟色胺 (5 HT)和 5 HT/5 羟吲哚乙酸 (5 HIAA)明显高于正常水平。针刺治疗在取得良好减肥效应的同时 ,肥胖大鼠纹状体Tyr、NA、DA、Trp、Ca2 + ATP酶水平明显回升 ,而 5 HT和 5 HT/5 HIAA水平明显回降。提示针刺对肥胖大鼠纹状体的良性作用可能是其实现减肥效应的中枢作用机制之一Objective: To investigate the mechanism of acupuncture in treatment of simple obesity. Methods: 33 rats were randomly and evenly divided into normal group, obesity control group and obesity + acupuncture group. Obesity model was established by feeding the rats with high fat diet. Bilateral “Zusanli" (ST 36) and “Neiting” (ST 44) were needled and stimulated with electrical pulses (10 Hz, 1.5 V, 10 min), once daily continuously for 12 days. At the end of the experiments, the fat in the pericardium, groin and around the kidney were separated and weighted. Contents of tyrosine (Tyr), dopamine (DA), noradrenaline (NA), tryptophan (Trp), 5 hydroxytryptamine (5 HT) and 5 hydroxyindoleacetic acid (5 HIAA)in the striatum tissue homogenate were detected with fluorospectrophotometry; the Na +, K + ATPase and Ca 2+ ATPase activity was determined with zymochemical method. Results: Tyr, DA, NA and Trp contents in obesity rats were lowered while 5 HT content and 5 HT/5 HIAA higher than those of normal group. After acupuncture treatment, the rat body weight was decreased, Tyr, NA, DA, Trp, Ca 2+ ATPase levels raised significantly (P<0.05,P<0.01); while 5 HT and 5 HT/5 HIAA reduced markedly (P<0.05). Conclusion: Acupuncture has a good action on reducing body weight via regulating monoamine neurotransmitter levels and raising ATPase activity in the striatum.关键词：针刺;单纯性肥胖症;纹状体;单胺类神经介质;ATP酶1189|0|4更新时间：2023-08-11
- 摘要：目的 :观察电针“会阳”、“中膂俞”对大鼠非细菌性前列腺炎诱发膀胱功能亢进的尿流动力学的影响。方法 :将大鼠分组并造成非细菌性前列腺炎 ,电针“会阳”、“中膂俞” ,分别于针刺后 3天、1 0天进行尿流动力学测定。结果 :电针“会阳”、“中膂俞”可调整大鼠膀胱尿流动力学指标 ,降低较高的膀胱内压 ,使低顺应性膀胱改善 ,抑制膀胱功能活动亢进 (频率、幅度 ) ,提高尿流率。结论 :电针“会阳”、“中膂俞”对大鼠非细菌性前列腺炎所致膀胱尿流动力学异常有一定的改善作用Objective: Observing the influence of electroacupuncture (EA) on the urodynamics in nonbacterial prostatitis rats. Methods: 30 SD rats were randomly and evenly divided into pseudo operation group, model group and model+acupuncture group. Nonbacterial prostatitis model was established by injecting 4% formaldehyde solution (0.1 mL)into the prostate. “Huiyang" (BL 35) and “Zhonglushu" (BL 29) were punctured and stimulated electrically for 30 min once daily,7 days altogether. The urodynamics was detected 3 and 10 days separately after acupuncture treatment. Results: 3 days following establishing model, in comparison with pseudo operation group, the basic pressure, pre micturition pressure, micturition pressure, residual urine, and the vesical contraction frequency and amplitude increased considerably (P<0.01, P<0.001), vesical compliance and uroflow rate lowered significantly (P<0.01,P<0.001), and the vesical volume and urination output decreased slightly.10 days after finishing prostatitis model, these indexes still kept a similar tendency. After acupuncture treatment, intravesical pressure was lowered, the vesical compliance improved, the increased vesical contraction frequency and amplitude were suppressed, and the uroflow rate was increased. Conclusion: Acupuncture treatment may ameliorate urodynamics in nonbacterial prostatitis rats.关键词：电针;“会阳”;“中膂俞”;非细菌性前列腺炎;尿流动力学1021|0|5更新时间：2023-08-11
- 摘要：本文通过临床观察及理论分析探讨针刺治疗中风偏瘫痉挛的取穴规律。本文对比观察了针刺手少阳经穴 (治疗组 )与针刺上肢阴经穴 (对照组 )治疗中风后上臂痉挛的疗效。结果 :治疗组取得了显著疗效 (P <0 .0 5 ) ,对照组无明显治疗效果 (P >0 .0 5 ) ,且治疗组疗效优于对照组 (P <0 .0 5 )。这与古代经典著作所记载的以阴经穴为主治疗拘急痉挛有所不同 ,值得进一步研究Objective: To study the regularities of acupoint selection in the acupuncture treatment of apoplectic hemiplegia. Methods: 62 apoplectic hemiplegia patients were randomly divided into treatment group (n=32) and control group (n=30). In the former group, Jianliao (TE 14), Naohui (TE 13), Xiaoluo (TE 12), Qinglengyuan (TE 11) and Waiguan (TE 5) of the upper limb, and Yanglingquan (GB 34), Zusanli (ST 36), etc of the lower limb were punctured. In control group, Jiquan (HT 1), Tianquan (PC 2), Chize (LU 5) and Neiguan (PC 6), and the same acupoints in the lower limb were punctured. Results: After 20 treatments, the muscular tension of treatment group was improved significantly in comparison with pre treatment (P<0.05), while that of control group had no apparent change in comparison with pre treatment (P>0.05); and the therapeutic effect of treatment group was superior to that of control group (P<0.05). Conclusion: The therapeutic effect of acupuncture of acupoints of Hand Shaoyang Meridian is superior to that of acupoints of the Yin meridian for treatment of upper limb spasm in apoplectic hemiplegia patients.关键词：中风;上臂痉挛;针灸疗法1698|0|4更新时间：2023-08-11
- 摘要：采用天灸疗法治疗 2 7例支气管哮喘患者 ,并与针刺法进行对照研究 ,观察两组的疗效及治疗前后血浆SP和VIP含量的变化情况。结果表明 ,两组的临床疗效无显著性差异。两组均能降低患者的血浆SP含量及提高VIP含量 ,但以实验组尤为明显Objective: To observe the effect of medicinal vesiculation (MV) on plasma substance P (SP) and vasoactive intestinal peptide (VIP) contents in patients with brachial asthma. Methods: 52 asthma patients were randomly divided into MV group (n=27) and acupuncture group (n=25). Feishu (BL 13), Dingchuan (EX B 1), Fengmen (BL 12), Dazhui (GV 14), etc were used. In MV group, Chinese medicinal herbs as Mahuang (Herba Ephedrae), Xixin (Herba Asari), Gansui (Radix Euphorbine Kansui), etc were processed into drug paste to be applied to the acupoints about 3～4 hr for adults and 1～2 hr for children, once in the first, the middle and the last of the three ten day periods of the hot season. In acupuncture group, these acupoints were punctured once every other day, beginning from the first day of the three ten day periods of the hot season on to the end of the hot season. Plasma SP and VIP were detected with radioimmunoassay. Results: After treatment, in MV group, 6 cases were under control, 9 had marked improvement, 8 had improvement and 4 had no effect, with an effective rate of 85.2%. In acupuncture group, 3 cases were under control, 8 and 8 had marked improvement and improvement respectively, and 6 had no effect, with an effective rate of 76%. Plasma SP level increased significantly and VIP decreased considerably in comparison with pre treatment in these two groups(P<0.05). Conclusion: Both MV and acupuncture can effectively improve asthma patients' symptoms, lower plasma SP and raise VIP level significantly.关键词：天灸;哮喘;P物质;血管活性肠肽1041|0|1更新时间：2023-08-11
- 摘要：目的 :观察针刺复合安氟醚全麻下实施食管癌根治术的临床应用效果。方法 :采用随机单盲法观察 ,70名食管癌患者随机分为A、B、C三组 ,A组 (n =2 4)即针刺复合安氟醚麻醉组 ,B组 (n =2 3)即电极板穴位刺激复合安氟醚麻醉组 ,C组 (n =2 3)即单纯安氟醚复合麻醉组 (对照组 )。三组静脉诱导和气管插管方法相同 ,麻醉维持静注芬太尼和维库溴铵用量相同 ,对安氟醚吸入浓度则根据病人血压、心率调节 ,术中A、B两组同时开放电刺激仪。结果 :A、B两组麻醉效果优于C组 ,A、B两组麻醉Ⅰ级率分别为 6 2 5 0 %和 6 5 2 2 % ,C组仅为 43 48% ,显著低于A、B两组。麻醉全程安氟醚用量A、B两组分别比C组平均少用 2 9 73%和 37 84%。上述结果具有统计学差异 ,P <0 0 1 ,显示针刺与全麻两者有协同作用。结论 :针麻复合安氟醚全身麻醉下施行食管癌手术 ,麻醉效果肯定 ,方法安全、新颖 ,并能减少安氟醚用量 ,降低麻醉费用 ,由于针麻可调节机体功能 ,能使并发症减少 ,故可作为食管癌手术的一种麻醉方法加以推广Objective: To observe the therapeutic effect of acupuncture combined with enflurane general anesthesia (ACEGA) for esophagectomy. Methods: 70 esophageal carcinoma patients were randomly divided into ACEGA group (n=24), electrical stimulation + enflurane general anesthesia (ES+EGA) group (n=23) and EGA control group (n=23). Xiayifeng Point and Sanyangluo (TE 8) to Ximen (PC 4) were punctured and stimulated with HANS (Apparatus) in patients of ACEGA group and only stimulated with electrical pulses in patients of ES+EGA group. Before enflurane anesthesia, all the patients accepted intramuscular injection of valium (0.1 g/50 kg) and atropine (0.5 mg/50 kg); after anesthesia, they accepted intravenous injection of fentany (2 μg/kg/hr) and vecuronium (0.03 mg/kg/0.5 hr). Results: In ACEGA, ES+EGA and EGA groups, the excellent rates of anesthesia accounted for 62.5% (15 cases), 65.22% (15 cases) and 43.48% (10 cases) respectively; the good rates were 20.83% (6 cases), 26.09% (6 cases) and 52.17% (12 cases) separately; and the poor rates were 16.67% (4 cases), 8.69% (2 cases) and 4.35% (1 case) separately. The dosages of the inhaled enflurane of ACEGA and ES+EGA were 29.73% and 37.84% lower than EGA group separately (P<0.05,P<0.01). There were significant differences between ACEGA and EGA and between ES+EGA and EGA groups in the anesthetic effect (P<0.01), showing a synergistic action between acupuncture/acupoint electrical stimulation and enflurane. Conclusion: Acupuncture combined with unflurane general anesthesia for esophagectomy has a good effect in regulating organic functional activities and reducing complications.关键词：针刺麻醉;安氟醚吸入麻醉;食管癌手术960|0|1更新时间：2023-08-11
- 摘要：目的 :针刺 (穴位电极刺激 )复合小剂量硬膜外麻醉胆囊切除临床研究。方法 :胆囊切除术共 1 0 6例 ,其中针硬组 (A组 ) 35例 ,电硬组 (B组 ) 35例 ,单纯硬膜外麻醉组 (C组 ) 36例。A组为针麻基础上复合小剂量硬膜外麻醉 ,B组为穴位电极刺激基础上复合小剂量硬膜外麻醉 ,C组为对照组 (单纯硬膜外麻醉 )。结果 :麻醉效果Ⅰ级率A组 80 0 0 %、B组 6 8 5 7%、C组 2 5 0 0 % ;硬膜外腔 1 5 %利多卡因用量 ,首次用量C组比A组、B组多用 35 43%、30 6 2 % (P <0 0 0 5 ) ;硬膜外腔首次用药量 9mL时A、B两组比C组多阻滞 2～ 3个神经节段 (A∶C、B∶C ,P <0 0 0 1 ) ;监测指标提示术中HR、BP、MBP、RR、TV、MV、SPO2 、ECG基本平稳 ,顺利、安全渡过手术。结论 :针刺(穴位电极刺激 )复合小剂量硬膜外麻醉可作为胆囊切除术麻醉方法之一。关键词：针刺麻醉;硬膜外复合麻醉;胆囊切除术1075|0|3更新时间：2023-08-11
- 摘要：本文共观察行择期胆囊切除术患者 46例 ,分为A组 (针刺复合小剂量硬膜外麻醉 ) 1 6例、B组 (穴位电极刺激复合小剂量硬膜外麻醉 ) 1 4例、C组 (单纯小剂量硬膜外麻醉 ) 1 6例三组。结果A、B两组麻醉优良率 (Ⅰ +Ⅱ级 )分别为 1 0 0 %和 92 90 % ,C组优良率 (Ⅰ +Ⅱ级 )为1 2 5 0 % ,A、B两组麻醉效果优于C组 (P <0 0 1 )。三组硬膜外腔每小时平均用药量 (1 5 %利多卡因 ,mL)A、B两组比C组分别减少 33 5 0 %和 34 0 0 % (P <0 0 1 ) ;第一小时容量及总容量A、B两组比C组减少均接近 30 % (P <0 0 1 )。说明针刺 (包括穴位电极刺激 )应用于胆囊切除术 ,既明显减少了硬膜外麻醉用药量 ,提高了麻醉效果 ,又弥补了针麻镇痛和肌松不全的不足 ,可作为胆囊切除术的麻醉方法之一Objective: To observe the effect of acupuncture combined with extradural administration of small dose of anesthetic for cholecystectomy. Methods: 46 cases of cholecystectomy were divided into acupuncture + epidural administration of anesthetic (A+EAA) group (n=16), electrical stimulation + EAA (ES+EAA) group (n=14) and EAA group (n=16). Bilateral Neiguan (PC 6) and Zusanli (ST 36) were used for acupuncture (2～3 mA, 2/15 Hz, sparse dense waves) and electrical stimulation (13 mA, 2/15 Hz, sparse dense waves) in A+EAA and ES+EAA groups. Before anesthesia, all the patients were given with intramuscular injection of atropine (0.5 mg/50 kg body weight) and luminal sodium (0.1 g/50 kg), and during operation, they were given with intravenous drip of meperidine (1 mg/kg). Epidural anesthetic used was lidocaine (1.5%, 5 mL) with epinephrine (E) for patients without contraindications or with E. Results: In A+EAA, ES+EAA and EAA groups, the excellent and good rates of anesthetic effect were 100.0% (16 cases), 92.9% (13 cases) and 12.5% (2 cases) respectively. The anesthetic effect of the former 2 groups was obviously superior to that of EAA group (P<0.05). The doses of lidocaine used every hour in A+EAA and ES+EAA decreased by 33.5.% and 34.0% respectively in comparison with EAA group, being significantly lower than that of EAA group (P<0.01). The volume in the first hour and the total volume of lidocaine in A+EAA and ES+EAA groups reduced by 30% compared with that of EAA group. During operation, blood pressure kept stable in A+EAA and ES+EAA groups while lowered in EAA group. Conclusion: Acupuncture and acupoint electrical stimulation combined with extradural injection of small dose of anesthetics can raise the anesthetic effect and meet the demands of cholecystectomy.关键词：针刺麻醉;硬膜外复合麻醉;胆囊切除术926|0|1更新时间：2023-08-11
- 摘要：目的 :探讨针刺复合硬膜外麻醉胆囊切除术胆心反射发生率。方法 :共观察择期胆囊切除术患者 45例 ,按麻醉方法不同 ,随机分为A、B、C三组 ,A组 (即针硬组 )、B组 (即电极组 )、C组(即对照组 )均要求硬膜外麻醉阻滞平面达到T4～ 1 1 以上 ,麻醉效果镇痛完全 ,肌肉松弛 ,牵拉反应轻 ,术中安静 ,根据术中探查、提胆囊、分离胆囊、切胆囊、缝胆囊床等主要手术步骤中HR、MAP、ECG的变化判断胆心反射是否发生。结果 :三组 (各 1 5例 )发生胆心反射共 1 5例 (31 1 1 % ) ,以提胆囊、分离胆囊及探查刺激胆囊发生胆心反射最多 (85 71 % ) ,按统一判断胆心反射标准评定三组胆心反射发生率无显著差异 (P >0 0 5 ) ,术前心电异常病例在术中胆心反射发生率较高 (5 7 1 4% ,P <0 0 5 )。结论 :本项研究三种麻醉方法还不足以抑制或阻断胆心反射发生 ,降低胆囊手术胆心反射发生率的有效方法尚需进一步探讨Objective: To observe the incidence rate of gallbladder cardiac reflex during cholecystectomy under acupuncture anesthesia combined with epidural administration of small dose of anesthetic. Methods: 45 cases of cholecystectomy were randomly and evenly divided into acupuncture + epidural administration of anesthetic (A+EAA), electrical stimulation (ES) + EAA and EAA groups. In A+EAA and ES+EAA groups, bilateral Neiguan (PC 6) and Zusanli (ST 36) were stimulated with electroacupuncture (2～3 mA, 2/15 Hz, sparse dense waves) and electrical stimulation (11～12 mA, 2/15 Hz, sparse dense waves), and the patients were also given with lidocaine (1.5%, 5 mL, till the blocked nerve segments being above T 4～11 ) with epinephrine (E) for patients without contraindications or with E. The anesthetic effect was asked to be painless, muscular relaxation, slight traction reaction, and the patient being quiet during operation. In the process of operation including exploring, lifting, separating and incising the gallbladder and suturing the gallbladder bed, the incidence of gallbladder cardiac reflex was assessed according to changes of heart rate (HR), mean artery blood pressure (MBP) and electrocardiogram (ECG). Results: In A+EAA, ES+EAA and EAA groups, the gallbladder cardiac reflex occurred in 4(26.67%), 5 (33.33%) and 5 (33.33%) cases respectively, mainly when the gallbladder was lifting, separating and exploring (85.71%). There was no significant difference among the 3 groups in the incidence of gallbladder cardiac reflex (P>0.05). In addition, in patients who had abnormal ECG before operation, the incidence of this reflex was higher during operation (57.14%). Conclusion: These three anesthetic methods are not capable of suppressing or blocking the occurrence of gallbladder cardiac reflex. The effective method for lowering this reflex needs to be researched further in the future.关键词：针刺麻醉;硬膜外复合麻醉;胆囊切除术;胆心反射900|0|1更新时间：2023-08-11
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