最新刊期
2001年第4期
- 摘要：目的 :比较不同部位穴位对实验性脑缺血疗效的影响。方法 :采用大鼠大脑中动脉暂时性缺血模型 ,比较电针头部的“百会”、“水沟”穴和远离头部的“环跳”、“昆仑”穴 ,对脑组织、血清NO含量的影响。结果 :缺血模型组与假缺血组比脑组织、血清NO含量显著增加 (P <0 .0 5) ;与缺血模型组比较 ,头部穴位组明显降低 (P <0 .0 1 ) ,肢体部穴位组降低无显著性意义 (P >0 .0 5) ;两穴位组之间比较 ,脑组织NO含量无显著性差异 ,血清NO含量有显著性差异 (P <0 .0 1 )。结论 :尚不能认为远近部位穴位的作用有差别Objective: To compare the curative effects of electroacupuncture (EA) of different acupoints on experimental cerebral ischemia. Methods: 41 SD rats were randomly divided into sham ischemia group (n=9), ischemia group (n=10), head acupoint group (n=11) and limb acupoint group (n=11). A rat model of temporary ischemia of middle cerebral arterial was used to compare the influences of EA of head acupoints "Baihui"(DU 20) and "Shuigou"(DU 26) and distant acupoints "Huantiao"(GB 30) and "Kunlun"(BL 60) on the NO contents of brain tissues and serum. The parameters of EA were frequency of 4～ 20 Hz, electric current of 3 mA, sparse dense waves and stimulation duration of 45 min. The serum and brain NO contents were detected with nitrate reductase method. Results: The NO contents of brain tissues and serum in ischemia group were significantly higher than those of sham ischemia group (P<0.05) and head acupoint group (P<0.01); but there were no significant differences between head acupoint group or limb acupoint group and sham ischemia group, or between head acupoint group and limb acupoint group in brain tissue NO contents; or between head acupoint group and sham ischemia group or limb acupoint group in serum NO contnts (P>0.05). Conclusion: EA of both head acupoints and limb acupoints can effectively lower cerebral ischemia reperfusion induced increase of NO level to reduce ischemic injury of the cerebral tissues. The results of the present study can not lead to a conclusion of existence of difference between EA of adjacent and distant acupoints in relieving cerebral ischema.关键词：脑缺血;电针治疗;脑组织及血清一氧化氮含量1073|0|2更新时间：2023-08-11
- 摘要：本文研究了D 半乳糖对大鼠空间学习记忆行为及对在体诱导海马齿状回LTP的影响 ,并观察了电针的干预作用。正常组每日皮下注射生理盐水 1mL ,模型组和电针组每日皮下注射D 半乳糖 ( 80 0mg/kg)共 6周 ,电针组从第 1 8天开始电针。空间学习记忆行为以Morris水迷宫潜伏期作为判定标准 ;应用记录单脉冲刺激穿通纤维在海马齿状回诱发的群体电位 ,测量高频刺激(HFS)前后单脉冲刺激诱发的电位幅值变化 ,将HFS前的记录作为对照值 ,进行组间比较。结果显示 :①模型组水迷宫潜伏期成绩明显低于正常组 ,电针组潜伏期成绩与模型组比显著提高 (P <0 .0 5) ;②HFS前记录显示 :三组间PS潜伏期和PS平均幅值无显著性差异 ,HFS后 ,1 0只正常组大鼠有 8只产生了LTP。其电位平均幅值增加为基线值的 1 3 5± 3 .9% ;1 0只模型组大鼠有 2只产生了LTP ,其平均值为基线值的 1 2 1 .4± 1 .4% ;电针组 9只大鼠有 5只产生了LTP ,其平均幅值为基线值的 1 3 1 .8± 5.3 %。提示 :D 半乳糖可损害大鼠的空间学习记忆能力、降低大鼠在体海马齿状回LTP的诱导率 ,降低LTP振幅 ;电针可提高模型动物的空间学习记忆能力 ,阻抑半乳糖对LTP诱导的损害作用Objective:To observe the influence of subcutaneous injection of D galactose on rat spatial learning and memory, induction of LTP in hippocampal dentate gyrus in vivo, and the effect of electroacupuncture (EA). Methods: 33 Wistar rats were randomly divided into normal control group (n=11), model group (n=11) and EA group (n= 11). In control group, normal saline (1 mL) was given to the rats subcutaneously every day, continuously for 6 weeks. In model group and EA group, subcutaneous injection of D galactose (800 mg/kg/d) was given to the rats daily and continuously for 6 weeks. From the 18th day on EA of ""Baihui"(GV 20) and "Dazhui"(GV 14) (5 mA, 10 Hz, 15 min) was conducted for rats of EA group. Spatial learning and memory ability was evaluated by latency of Morris water maze. Following anesthesia with 20% urethane 1.0 g/kg(i.p.), according to Paxinos' Rat Brain Stereotaxic Atlas, a bipolar stimulating electrode was inserted into the site (A: 7.5 mm; R:4.0 mm) 4 mm below the cortex and an induction electrode inserted into the site (A: 3.5 mm, R: 2.0 mm) for recording potentials of hippocampal dentate gyrus. The average value of 20 population spikes (PS) induced by single pulse stimulation of perforate path fiber was used as the baseline value for detecting the changes of potential amplitude before and after high frequency stimulation (HFS). Results: The latency of Morris water maze of model group was longer than that of control group, suggesting impairment of the learning and memory induced by D galactose. Compared with model group, the latency of EA group was significantly shorter, suggesting an inhibitory effect of EA on D galactose injury (P<0.05). Before HFS, no difference was found among the three groups in the latency and the mean amplitude value of PS; while after HFS, in control group, LTP appeared in 8 of 10 rats and the mean amplitude value of PS increased to 135.0±3.9% in comparison with the baseline value. In model group, LTP occurred in 2 of 10 rats and the mean amplitude value of PS increased to 121.4± 1.4%. In EA group, LTP generated in 5 of 9 rats and the mean amplitude value of PS increased to 131.8±5.3%. The incidence of LTP induction of rats in model group was significantly lower than that of control group(P<0.05).The ratio of PS amplitude after HFS 60 min and before HFS in EA group was higher than that of model group(P<0.05). The incidence of LTP was increased in EA group, but had no significant difference. Conclusion: EA can suppress subcutaneous injection of D galactose induced learning and memory impairment, improve its resultant incidence of LTP induction and increase PS amplitude in the rat.关键词：电针;D-半乳糖;长时程增强;海马齿状回;学习记忆障碍模型1039|0|4更新时间：2023-08-11
- 摘要：目的 :阐明杏仁及中枢阿片肽类递质在躯体传入冲动对下丘脑室旁核 (PVN)兴奋诱发的心血管活动的调节效应中的作用。方法 :采用电刺激及多管玻璃微电极脑内微量注射法 ,观察在侧脑室或同侧杏仁中央核 (ACe)微量注射阿片受体阻断剂纳洛酮后 ,躯体传入冲动对兴奋PVN诱发的心血管反应的影响。结果 :电刺激一侧PVN后 ,平均动脉压 (MAP)升高。电刺激腓深神经(DPN)对上述反应有部分抑制作用 (P <0 .0 1 ) ,DPN对升压的抑制百分比为 43 .2 9%。电刺激DPN也能抑制一侧PVN微量注射L 谷氨酸钠 (L SodiumGlutamate,Glu) 1 0 0nL引起的升压效应 ,抑制百分比为 64.58%。侧脑室注射纳洛酮 ( 3 0 μg/ 1 5μL)可部分阻断DPN的上述抑制作用 ,DPN对升压的抑制百分比由 56.67%下降到 1 3 .79% ,抑制取消了 42 .88%。杏仁注射纳洛酮( 2 0 0ng/ 1 0 0nL) 也削弱刺激DPN对刺激PVN诱发的升压反应的抑制作用 ,DPN对升压的抑制百分比由 60 .1 9%下降到 2 1 .0 5% (P <0 .0 1 ) ,抑制削弱了 3 9.1 4 %。结论 :杏仁及中枢阿片肽参与DPN传入冲动对PVN中枢性心血管反应的抑制作用Objective: To analyze the role of amygdala and central opioid peptide in somatic afferent input induced suppression of the cardiovascular response to electrical stimulation of the paraventricular nucleus of the hypothalamus (PVN). Methods:SD rats anesthetized using 20% urethane (1.0～ 1.2 g/kg) were used in this study. In accordance with Paxinos and Watson's Rat Brain Stereotaxic Atlas, a bipolar stainless stimulating electrode was inserted into PVN for giving central electrical stimulation. Multibarreled micropipettes (about 50 μm in tip diameter) were employed for microinjection of L sodium glutamate, naloxone or normal saline into the lateral ventricule or amygdala. Deep peroneal nerve (DPN) was stimulated with electric current 0.3 ～0.4 mA, frequency of 4 Hz, 0.5 ms of duration of wave wideth and 5 min of stimulation duration. The stimulating parameters of PVN were 0.1～ 0.3 mA, 80 Hz, 0.5 ms (wave wideth) and 10 sec (duration of stimulation). Results: Electrical stimulation of PVN caused increase of mean arterial pressure (MAP). Electrical stimulation of DPN could significantly inhibit PVN stimulation induced increase of MAP(P<0.01), with the inhibitory percentage of pressor response being 43.29%. Stimulation of DPN could also inhibit the pressor response induced by introducing L sodium glutamate (100 nL in 0.5 M) into PVN. The inhibitory percentage of pressor response was 67.11%. Administration of naloxone (30 μg/15 μL) into lateral ventricle of brain reduced the inhibitory effect of DPN stimulation. The inhibitory percentage decreased from 56.67% to 13.79%. Administration of naloxone (0.2 μg/0.1 μL) into amygdalaalso reduced the inhibitory effect of DPN stimulation. The inhibitory percentage decreased from 60.19% to 21.05% (P<0.01). Conclusion: Central opioid receptor and amygdala are involved in the inhibitory effect of DPN stimulation on central pressor response.关键词：受体;阿片;杏仁;下丘脑室旁核;腓深神经;中枢性升压反应985|0|3更新时间：2023-08-11
- 摘要：目的 :本研究探讨一氧化氮 (NO)在糖尿病神经病变发病机制中的作用及针刺的效应。方法 :用腹腔注射链脲佐菌素 (Streptozotocin ,STZ)建立糖尿病动物模型 ,采用免疫组织化学方法观察糖尿病小鼠脑内神经原性一氧化氮合酶 (nNOS)免疫阳性神经元在脑内的分布。结果 :糖尿病可引起小鼠脑内各区 (包括大脑皮层、海马、下丘脑和杏仁核 )nNOS免疫阳性神经元表达增多 ,大脑皮层和杏仁核内的nNOS表达增加有显著性意义 ,在杏仁核和梨状皮质过度区也有大量nNOS阳性神经元分布 ,而针刺能抑制糖尿病引起的nNOS免疫阳性神经元表达的增加 ,这种抑制作用在大脑皮层和杏仁核具有显著性意义。另外在糖尿病小鼠的海马、下丘脑中可见到nNOS阳性神经元 ,也较正常组多 ,针刺右侧“太溪”对nNOS阳性神经元的增加也有一定的抑制作用 ,但均未达显著性意义。结论 :糖尿病可增加脑内nNOS免疫阳性神经元表达 ,针刺可抑制这种nNOS阳性神经元的增加 ,有类似NOS抑制剂的作用 ,可拮抗nNOS的神经毒性Objective: To explore the effect of nitric oxide (NO) in the pathogenesis of diabetic neuropathy and the effect of acpuncture on nitric oxide synthase (NOS) expression in the brain. Methods: Twenty one Kunming male mice wererandomly divided into normal control group (n=6), diabetes group (n=7) and acupuncture group (n=8). NOS expression was displayed using immunohistochemical technique. Diabetes mouse model was produced by intraperitoneal injection of streptozotocin (STZ). Right "Taixi" (KI 3) was punctured and stimulated by twirling the acupuncture needle continuously for 2 min with hand. The treatment was given once every other day, continuously for 9 sessions. Results: After inducing diabetes for 6 weeks, the positive neurons of nNOS in different regions (cerebral cortex, hippocampus, hypothalamus, amygdaloid nucleus, the transitional area between the amygdala and the piriform cortex, the basolateral nucleus and the anterior region of the amygdala) of the brain increased in diabetes group than those of normal control group, especially being significant in the cerebral cortex and amygdaloid nucleus (P<0.01). But in acupuncture group, the positive neurons in the above mentioned brain regions particularly in cerebral cortex and amygdala were fewer in number than those of diabetes group.(P<0.01). Conclusion: In diabetes mice, acupuncture may suppress nNOS expression that mimics the effect of NOS antagonists in resisting nNOS toxicity.关键词：针刺;糖尿病小鼠;一氧化氮合酶;免疫组织化学1114|0|0更新时间：2023-08-11
- 摘要：本实验采用无水乙醇灌胃造成胃粘膜损伤后 ,分别电针足阳明经“四白”、“梁门”、“足三里”三个不同段代表穴 ,及“足三里”外 2cm的对照点 ,观察其对胃粘膜损伤后的细胞保护作用 ,以证实足阳明经与胃的相关性。结果发现 ,分别电针“四白”、“梁门”、“足三里”7日后 ,均能使胃粘膜损伤指数显著降低。电针“足三里”后 ,胃液及胃粘膜PGE2 与其他各组比较均显著增高 (P <0 0 5或 0 0 1 ) ,血清NO与模型组、电针“足三里”外 2cm组比较亦见明显增高 (P <0 0 5) ;电针“四白”、“梁门”、“足三里”以及空白组胃粘膜EGF与未经电针的模型组比较均有显著差异 (P <0 0 5或0 0 1 )。提示 ,电针家兔足阳明经不同节段的腧穴均对胃粘膜损伤细胞有保护作用 ,其中以“足三里”最满意 ,说明足阳明经与胃具有相关性 ,与此同时 ,同一经脉的穴位对相关脏腑的作用亦有着相对的特异性Objective: To observe the effect of electroacupuncture (EA) of "Sibai" (ST 2), "Liangmen" (ST 21) and "Zusanli" (ST 36) on gastric mucosal tissues in alcohol induced gastromucosal lesion rabbits for analyzing the relationship between Foot Yangming Meridian and stomach. Methods: 48 rabbits were randomly and evenly divided into "Sibai" (ST 2), "Liangmen" (ST 21), "Zusanli" (ST 36), non acupoint, model and control groups. Gastric mucosal injury model was induced by intragastric infusion of dehydrated alcohol (2.35 mL/kg). The non acupoint was located at the site about 2 cm lateral to "Zusanli" (ST 36). EA parameters were frequency of 4～20 Hz, pulse width 0.5 ms, voltage 60 V (peak peak value, no load), and stimulation duration 20 min. The treatment was given once daily, continuously for 7 days. Prostaglandin (PG) E 2, gastrin (GAS), epidermal growth factor (EGF) of plasma, gastric juice and gastric mucous tissue concentrations were detected with radioimmunoassay and serum nitric oxide (NO) level was determined by using ultraviolet spectrophotometry. Results: After EA of the above mentioned 3 acupoints, the gastric mucosal injury index decreased significantly in comparison with non acupoint group (P<0.05). PGE 2 levels in plasma, gastric juice and mucous tissues in EA of "Zusanli" group were all obviously higher than those of the rest groups (P<0.05 or 0.01); and serum NO level of "Zusanli" group was also higher than that of the rest groups, markedly higher than that of model group and non acupoint group (P<0.05). After generation of gastric mucosal injury, EGF level of gastric mucous tissues decreased significantly in comparison with control group. While gastric mucosal EGF levels in "Sibai", "Liangmen" and "Zusanli" and control groups were all obviously higher than that of model group (P<0.05 or 0.01), but no significant difference was found between non acupoint group and model group (P>0.05). Conclusion: These findings suggest that EA of different acupoints of Foot Yangming Meridian has a protective effect on gastric mucosal lesion; among those acupoints, and the effect of "Zusanli" (ST 36) is the best. It indicates that there is a close relationship between Foot Yangming Meridian and stomach.关键词：“四白”;“梁门”;“足三里”;胃粘膜损伤;细胞保护作用1493|0|11更新时间：2023-08-11
- 摘要：目的 :本实验以猫胸段具有自发活动的背角神经元的放电频率为指标 ,观察了胃扩张和电刺激左侧内脏大神经引起的神经元电活动变化以及电针左侧“足三里”穴和“三阴交”穴的影响。方法 :采用玻璃微电极脑外记录神经元的电活动。工作参数如下 ,电针穴位 :3V ,3 0Hz,方波 ,连续 1 0sec;内脏大神经 :1mA ,双脉冲 ,间隔 4ms,波宽 2 0 0 μs,连续 1 0sec;胃充盈 :8mmHg( 1 0 64kPa)。结果 :①在所观察的 2 4个神经元中 ,1 3个对胃扩张刺激呈增频反应 ,1 1个为减频反应 ;电针“足三里”穴能够翻转或拮抗这种改变 ,即抑制增频神经元 ( 62 .50± 6.52 ,与 2 3 .3 0± 6.86相比 ,P <0 .0 5,脉冲个数 ,M±SE ,下同 ) ,兴奋减频神经元 ( 1 9.1 0± 4.72 ,与 71 .60± 1 7.50相比 ,P <0 .0 5)。电针“三阴交”穴具有类似功能 ,但作用较弱 ,无统计学意义 ;②在 2 1个 ( 2 1 / 2 4)神经元中 ,电刺激左侧内脏大神经可兴奋 1 3个神经元 ,抑制 7个神经元 ,其中 3个为完全抑制 ;③刺激内脏大神经同时电针“足三里”穴可见到后者对前者显著的抑制效应。结论 :①躯体与内脏的信息在脊髓水平汇聚并整合 ,进而通过改变内脏传出神经的活动达到调整治疗的目的 ;②针灸具有双向的调节功能Objective: To observe the influence of stomach dilation or electrical stimulation of the left grea ter splanchnic nerve (GSN) on the discharge frequency of dorsal horn neurons of the thoracic (8th) spinal cord and to compare the effects of electroacupuncture of "Zusanli"(ST 36) and "Sanyinjiao"(SP 6) on the electrical activity of dorsal horn neurons in the cat. Methods: Healthy cats anesthetized with mixture solution of urethane (500 mg/kg) chloralose (50 mg/kg) (i.p.) were used in the present study. Glass micropipettes with tip diameter being about 1～ 2 μm were employed for recording the electrical activity of dorsal horn neurons of the spinal cord. After opening the abdominal cavity and making an incision at the junction between the pylorus and duodenum, a plastic balloon was inserted into the stomach, with the other end being connected to air pump and pressure transducer. The left GSN was isolated and placed on a silver bipolar stimulating electrode. Left "Zusanli"(ST 36) and "Sanyinjiao"(SP 6) were stimulated with an Electroacupuncture Apparatus (ZYZ ZOZG1) by setting the stimulating parameters being 3 V, 30 Hz and duration of 10 sec.Results: ① Of all the 24 neurons recorded, after gastric dilation, 13 were excited and 11 inhibited. Electroacupuncture of "Zusanli" (ST 36) may turn over these changes, that is, to reduce the firing rate of the excited neurons (62.50 ±6.52 vs 23.30±6.86, P<0.05, M±SE, the same below) or to increase the firing rate of the inhibited neurons (19.10±4.72 vs 71.60±17.50, P<0.05). Electroacupuncture of"Sanyinjiao"produced the similar but weaker effect and there existed no statistical significance between pre and post EA (P>0.05). ② Of the 21 (21/24) neurons, following electrical stimulation of GSN, 13 neurons were excited and 7 inhibited. 3 (3/7) neurons were totally inhibited. Electroacupuncture at "Zusanli" (ST 36) produced remarkable inhibition on the discharge increase of dorsal horn neurons induced by the electrical stimulation of GSN. Conclusion: ①The somatic and visceral message may converge to the spinal cord; ② Acupuncture has a bi directional regulatory action on the nocuous input of the internal organs. The research provided new evidence for the theory of "relationship between acupoints and visceral organs" in TCM.关键词：胃扩张;脊髓背角神经元;经穴脏腑相关;“足三里”穴1192|0|1更新时间：2023-08-11
- 摘要：目的 :探讨原癌基因c fos在穴位电针对胃运动影响中的表达及其意义。方法 :采用免疫组织化学方法及电生理的方法 ,观察电针刺激“足三里”等不同穴位 ,c fos在中枢延髓的孤束核(NTS)及迷走神经背运动核 (DMV)中的表达 ,同时采用浆膜法检测胃电变化情况。结果 :电针刺激“足三里”等不同穴位c fos在NTS及DMV中的表达情况不同 ,且胃电也发生较为明显的变化。结论 :穴位电针对胃运动具有调节作用。以c fos的表达作为激活标志 ,提示这种调节作用可能是通过对NTS及DMV神经元的激活而实现的Objective:To observe c fos gene expression and analyze its significance in electroacupuncture (EA) regulation on gastric motor in rats. Methods:64 SD rats were randomly divided into "Zusnali" (ST 36) group (n=8), "Shangjuxu" (ST 37) group (n=8), non acupoint group (n=8) and control group (n=8). By using immunohistochemical technique (ABC method), c fos gene expression in the Dorsal motor nucleus of the vagus nerve (DMV) and nucleus tractus solitary(NTS) was displayed. After anesthesia (846 mixture, 0.1 mL/100 g body weight), the rat's abdominal cavity was opened and an induction electrode was implanted at the serous coat of the anterior wall of the stomach (about 0.3 ～0.5 cm to the pylorus) for recording EGG(electrogastrography). Left "Zusanli"(ST 36) and "Shangjuxu"(ST 37) were stimulated with EA by setting the stimulating parameters being frequency of 50 Hz, voltage of 20 V and duration of 30 min. Results:In control group, c fos positive neurons in DMV and NTS were few. After EA of "Zusanli"(ST 36) and "Shangjuxu"(ST 37), c fos positive neurons increased considerably in number in comparison with those of control group (P<0.01). While those of "Zusanli"group were obviously more than those of "Shangjuxu" group (P<0.01) but no significant difference was found between non acupoint group and control group (P>0.05). Simultaneously, changes (increase of both frequency and amplitude after EA) of EGG of the four groups presented the same tendency. Conclusion: EA can adjust gastrtic motor function, which may be closely associated with EA activating neurons of NTS and DMV.关键词：c-fos基因;电针;胃运动830|0|7更新时间：2023-08-11
- 摘要：目的 :观察电针“足三里”、“三阴交”穴对胃酸分泌的影响及血浆、胃液GAS、EGF的变化 ,比较阴阳经穴对脏腑功能影响的差异关系 ,探讨电针调节胃酸分泌的机制。方法 :75只SD大鼠随机分为空白对照组、非经非穴组、“足三里”穴组、“三阴交”穴组和联合穴位组 ,测定空腹胃液量、胃液 pH值及胃液酸度 ,同时放免法测定血浆和胃液胃泌素 (GAS)、表皮生长因子 (EGF)含量。结果 :电针后“足三里”穴组和“三阴交”穴组胃液量均显著减少 (P <0 .0 1 ,0 .3 0± 0 .1 1mL ,0 .43± 0 .0 7mL ,与 0 .63± 0 .1 2mL对照 ) ,胃液 pH值变化不大 ,前者胃液酸度明显下降 (P <0 .0 5,3 0 .5± 3 .3mmol/L ,与 40 .9± 8.9mmol/L对照 ) ,后者无变化。二穴联合酸度显著下降 (P <0 .0 1 ,2 8.3± 4.5mmol/L ,与 40 .8± 8.9mmol/L对照 )。“足三里”穴组胃液胃泌素显著下降 (P <0 .0 5,2 72 .6± 60 .8ng/L ,与 2 84.3± 3 1 .9ng/L对照 ) ,EGF显著升高 (P <0 .0 1 ,3 .2 2± 1 .1 μg/L ,与 1 .8± 0 .4μg/L对照 ) ,“三阴交”穴组胃液GAS升高 (P <0 .0 1 ,453 .9± 8.1 1ng/L ,与 2 84.3± 3 1 .9ng/L对照 ) ,胃液EGF也升高 (P <0 .0 1 ,2 .6± 0 .2 μg/L ,与 1 .8± 0 .4μg/L对照 ) ,各组胃液EGF和GAS均无相关性 ,“Objective: To explore the regulative effect of electroacupuncture(EA) on gastric acid secretion and its mechanisms. Methods: 75 SD rats were randomized evenly into 5 groups: control group, non acupoint group, "Zusanli"(ST 36) group, "Sanyinjiao"(SP 6) group and "Zusanli"+ "Sanyinjiao" group. We had measured the pH of fasting gastric juice as well as its volume and acidity. Meanwhile, radioimmunoassay was applied to detect the contents of gastrin (GAS) and epidermal growth factor (EGF) in plasma and gastric juice. EA parameters were frequency of 2 ～15 Hz, voltage of 20～ 35 V (blank load), dense sparse waves and duration of 30 min. Results: After EA, the volume of fasting gastric juice in both of "Zusanli" group and "Sanyinjiao" group decreased significantly. PH values of the gastric juice of these group had no apparent changes. The acidity of "Zusanli" group and "Zusanli"+ "Sanyinjiao" group lowered significantly (P< 0.05～0.01) . GAS content of gastric juice in "Zusanli" group decreased obviously (P<0.05) while EGF in the gastric juice increased significantly (P<0.01). The contents of both EGF and GAS in gastric juice in "Sanyinjiao" group increased significantly. There was no interrelation between EGF and GAS of gastric juice in the changes. In"Zusanli"group, despite no significant decrease of plasma EGF was found, there still was a positive correlation between plasma EGF and GAS (r=0.6213, P<0.05). In "Zusanli"+ "Sanyinjiao" group, there wasa negative correlation between plasma EGF and GAS decrease (r=-0.72, P<0.01). There was correlation between plasma EGF and GAS was found in "Sanyinjiao" group and non acupoint group. Conclusion: EA of both "Zusanli" and "Sanyinjiao" can suppress gastric acid secretion singly and also has a synergic action. EA of "Zusanli" can function in suppressing release of GAS and accelerating the secretion of gastric EGF, while EA of "Sanyinjiao" may promote the release of both GAS and EGF in the stomach. There exists certain specificity in the effect of different acupoint stimulation on gastrointestinal hormone activity.关键词：电针;足三里;三阴交;胃酸分泌;胃泌素;表皮生长因子1064|0|4更新时间：2023-08-11
- 摘要：目的 :观察咪唑啉受体在痛觉调制和针刺镇痛中的作用。方法 :本文以辐射热照射致甩尾反射潜伏期作为测痛的指标 ,采用蛛网膜下腔注射 (ith)咪唑啉受体的激动剂和拮抗剂的方法观察咪唑啉受体对痛阈和针刺镇痛效应的影响。结果 :ith可乐宁和电针双侧“次”穴可产生明显的镇痛效应 ,均可被事先注射咪唑啉受体的拮抗剂苯恶唑 (Idazoxan)所阻断。结论 :说明激活咪唑啉受体可能是可乐宁和电针镇痛效应的共同脊髓机制 ,但是没有观察到可乐宁明显加强针刺镇痛的协同作用Objective: To observe the effect of spinal subarachnoid injection of clonidine and idazoxan on pain reaction and acupuncture analgesia for analyzing the action of imidazoline receptors in acupuncture analgesia. Methods: SD female rats (220±15 g) were used in the present study. The latency of tail flick in response to radiation heat stimulation was employed as the pain index. Bilateral "Ciliao" (BL 32) wee punctured with filiform needles and stimulated electrically by setting the stimulation parameters being 1 ～2 mA (electric current), 50 Hz (frequency) and duration of 20 min. Under urethane anesthesia (1 g/kg, i.p.), an polyethylene catheter was implanted into the rat subarachnoid space of the lumbar spinal cord for microinjection of clonidine (5, 25 or 50 μg /10 μL), normal saline (NS, 10 μL ) or idazoxan (60 μg/10 μL), a selected antagonist of imidazoline receptors. Every administration of the aforementioned drugs consisted of 6 ～8 cases of rats. Results: After microinjection of clonidine (25 and 50 μg /10 μL) the pain threshold increased significantly in comparison with basal value (P< 0.05), that was blocked completely by pre administration of idazoxan (60 μg/10 μL). Following EA of "Ciliao" (BL 32), the pain threshold also increased significantly and this effect of EA could be blocked partially by pre treatment with idazoxan (60 μg). There was a significant difference between idazoxan and NS pre treatment in influencing the analgesic effect of EA (P<0.01). Despite no significant effect of subarachnoid injection of 5 μg clonidine on pain threshold or acupuncture analgesia was found, if used together with morphine, it could apparently strengthen the analgesic effect of morphine (50 μg/10 μL), which was strikingly stronger than that of simple microinjection of morphine (P< 0.01). Conclusion: The above mentioned results suggest that activation of the spinal imidazoline receptors is involved in both clonidine antinociception and acupuncture analgesia.关键词：咪唑啉受体;可乐宁;苯恶唑;痛觉调制;针刺镇痛1068|0|0更新时间：2023-08-11
- 摘要：目的 :观察电针对帕金森病大鼠黑质TH含量的影响。方法 :将 6 OHDA注入黑质造成单侧黑质损毁的帕金森病大鼠模型 ,并用免疫组织化学法检测正常组、假手术组、模型组、电针组黑质TH阳性神经元数目。结果 :帕金森病大鼠损毁侧SNC、SNL的TH阳性神经元数目显著低于正常组 (P <0 .0 1 )及未损毁侧 (P <0 .0 1 ) ,电针可有效地增高帕金森大鼠损毁侧SNC、SNL的TH阳性神经元数目 (P <0 .0 1 ,P <0 .0 5)。结论 :帕金森病大鼠黑质多巴胺能神经元合成多巴胺的功能低下 ,电针可有效地改善这一病理状态Objective: To observe the effect of electroacupuncture (EA) on the content of TH in mesencephalic substantia nigra (MSN) of PD rats. Methods: 40 SD rats were randomly divided into normal control group (n=10), sham operation group (n=10), EA group (n=10) and Parkinson's disease (PD) group (n=10). The PD rat models was made by injecting 6 OHDA (3.4 μL) into MSN (to injure unilateral part) according to the modified Ungerstedt's method. "Fengfu" (GV 16) and "Taichong" (LR 3) were punctured with filiform needles and then the needle handles were connected to a G6805 Electroacupuncture Therapeutic Apparatus for stimulating the acupoints with continuous waves, frequency of 120 rpm, appropriate strength (inducing slight muscular tremor) and duration of 30 min, once daily continuously for 7 days. The sections of the midbrain tissues were stained with ABC method for detecting positive TH neurons. Results: In PD rats (PD group), the number of TH positive neurons in SN compact (SNC) area, SN lateral (SNL) area of the injured side were obviously lower than those of normal group and sham operation group (P<0.01). While no significant changes was found in TH positive neuron number of the uninjured side. In EA group, the number of TH positive neurons of SNC and SNL on the injured side were markedly higher than that of PD group on the injured side (P<0.05) but significantly lower than that of normal group and uninjured side of the same group (P<0.05). Conclusion: Results suggest that EA may improve the hypofunctional state in dopamine synthesis of MSN dopaminergic neurons in PD rats.关键词：电针;黑质;帕金森模型大鼠;TH阳性神经元904|0|3更新时间：2023-08-11
- 摘要：为研究“气至病所”的外周神经体液机制 ,本课题借用福尔马林试验 ,在大鼠一侧足底皮下注射 5%福尔马林 50 μL作为局部病灶 ,观察电针“阳陵泉”、“外丘”穴 (刺激参数为 1～ 3V ,4～1 6Hz,3 0min)对大鼠自发痛行为反应、相关皮瓣浸泡液内 5 羟色胺 ( 5 HT)、肾上腺素 (E)、去甲肾上腺素 (NE)含量的影响。结果表明 ,大鼠一侧足底注射福尔马林后 0～ 80min出现明显的自发痛行为反应 ,电针能显著减少大鼠福尔马林试验后的自发痛行为反应 ;大鼠一侧足底注射福尔马林后60min相关皮瓣浸泡液内 5 HT、NE、E含量显著增加 ,电针能拮抗大鼠福尔马林试验后相关皮瓣浸泡液内 5 HT、NE、E含量的上升。结果提示 ,循经电针可通过外周神经体液机制促使“气至病所” ,减少局部炎症介质的释放和交感传出导致的痛过敏反应Objective:To research the peripheral neurohumoral mechanism of "Qi Zhi Bing Suo"In the rat. Methods: Thirty Wistar male rats were randomly divided into normal saline (NS) group (n=10), formalin (F) group (n=10) and electroacupuncture (EA)+ F group (n=10) for observing pain behavior changes ; other 40 Wistar male rats were divided into NS, EA+NS, F and EA+F groups with 10 rats in each group. In these rats of each group, subcutaneous injection of NS 50 μL or 5% formalin 50 μL was performed in rats' plantar center respectively for inducing local formalin focus and saline control. EA of"Yanglingquan"(GB 34) and "Waiqiu"(GB 36) on the focus side was given to the rats of EA group with stimulation parameters of 1～3 V,4～16 Hz and 30 min for observing the analgesic effect. The contents of 5 hydroxytryptamine (5 HT),Norepinephrine (NE) and Epinephrine (E) in the skin flap soak liquid were detected using fluorospectrophotometry. Results: 0～ 80 min after subcutaneous injection of formalin, the evoked spontaneous pain behavior (shortening leg, licking or biting the red swelling focus) appeared, while after EA the number of leg shortening and biting duration decreased significantly (P<0.05). Sixty minutes after subcutaneous injection of formalin, the contents of 5 HT, NE and E in the skin flap soak liquid all increased significantly in comparison with those of NS and EA+NS groups (P<0.05～ 0.01), while those of F group were significantly higher than those of EA+F group, indicating an inhibitory effect of EA on release of 5 HT, NE and E from the skin flap. Conclusion: The results suggest that EA of acupoints could promote"Qi Zhi Bing Suo" (qi reaching the focus) along the focus related meridian via peripheral neurohumoral mechanism and reduce release of local inflammatory mediators and lighten hyperalgesia caused by sympathetic efferent.关键词：气至病所;福尔马林诱发痛;5-羟色胺;肾上腺素;去甲肾上腺素;大鼠1005|0|4更新时间：2023-08-11
- 摘要：应用 6 OHDA化学切除外周交感神经轴突纤维 ,观察艾灸对正常和CY(环磷酰胺 )免疫抑制小鼠免疫功能的影响。结果 :应用 6 OHDA及CY后 ,小鼠胸、脾指数及IL 1、IL 2含量均低于正常组水平 ;艾灸治疗能提高小鼠免疫功能 ,改善其免疫抑制 ,上述指标均高于各自对照组 ,但仍低于交感神经完整的艾灸组。结果表明 ,6 OHDA损毁外周交感神经末梢后 ,艾灸增强与调节免疫的作用被削弱或部分阻断 ,提示交感神经参与艾灸对免疫的调节。关键词：艾灸;交感神经;6-羟多巴胺;环磷酰胺;免疫功能1253|0|5更新时间：2023-08-11
- 摘要：本文观察电针“足三里”穴对吗啡戒断大鼠β EP免疫调节作用的影响 ,探讨针刺祛毒扶正的作用机理。结果表明 ,电针组血清和垂体内 β EP含量增加 ,IL 1、IL 2升高 ,免疫器官重量和体重比吗啡对照组明显增加 ,垂体β EP与IL 1之间呈正相关趋势。提示针刺脱毒扶正 ,改善戒断症状的作用可能与β EP对细胞因子的良性调节有关Objective: To observe the effect of electroacupuncture (EA) of"Zusanli"(ST 36) on the immunoregulating action of β EP in rats with morphine abstinence syndrome for studying the mechanism of acupuncture in dispelling toxin and reinforcing body resistance. Methods: A total of 24 rats were divided into control group (n=8), morphine group (n=8) and EA group (n=8). Rat morphine abstinence syndrome model was made by intramuscular injection of morphine (20 mg/kg for the first day, double, 3 times and 4 times for the following 3 days, and 100 mg/kg for the 5th ～8th day). EA (2 Hz ～100 Hz, 10 V, continuous waves) was applied to "Zusanli" (ST 36), once daily and continuously for 8 days. Serum IL 1, IL 2, β EP contents and β EP level of hypophysis were detected using radioimmunoassay.Results: The results showed that in comparison with morphine group, the rat body weight, spleen weight and thymus gland weight, β EP in serum and hypophysis, Serum IL 1 and IL 2 of EA group and normal group increased significantly (P<0.05 ～0.001). There was a positive correlation between β EP of hypophysis and IL 1 in EA group. Conclusion: Results suggest that the effect of acupuncture in improving symptoms of abstinence symposium might be related to favorable regulation of β EP on cellular factors.关键词：电针;戒断大鼠;β-EP免疫调节;血清IL-1及IL-21075|0|1更新时间：2023-08-11
- 摘要：本文以电针为主 ,取水沟、百会、三阴交、内关、人迎、后溪穴 ,配合西医对症治疗迁延性昏迷 2 4例 ,结果恢复良好者 3例 ,中度残疾者 1 0例 ,重度残疾者 6例 ,持续植物人状态者 4例 ,死亡 1例 ,总有效率 79 2 %。提示电针综合疗法可显著提高本病的治愈率 ,减少病残率Objective: To observe the therapeutic effect of combined treatment of protracted coma with acupuncture and Western medicines. Methods: 24 protracted coma patients(17 males and 7 females ranging in age from 30 to 80 years, the shortest duration of disease being 11 days and the longest 45 days) are subjected into this observation. Acupoints used are Shuigou(GV 26), Baihui(GV 26),Sanyinjiao(SP 6), Neiguan(PC 6), Renying(ST 9) and Houxi(SI 3), stimulated with strong needling manipulation by twirling the needles in a large amplitude and then stimulated electrically using G6805 Electroacupuncture Therapeutic Apparatus for 30～45 min. The treatment is given twice every day, with 20 sessions being a therapeutic course. Western medicines for controlling cerebral edema, lowering intracranial pressure, preventing complications, nourishing nerve and promoting metabolism as CDPC, NGF, piracetum, etc. are employed, in combination with high pressure oxygen treatment, speech, music and visual stimulation.Results: After 1～2 months' treatment, 11 cases recover their consciousness, after 2～3 months' treatment, other 8 cases gain consciousness while 4 cases still keep persistent vegetative state and one case died. Those patients gaining consciousness contain 3 cases of good recovery, 10 cases of medium disablement and 6 cases of severe disablement. The total effective rate is 79.2%. Conclusion: This comprehensive treatment for protracted coma with electroacupuncture, Western medicines, etc can significantly raise the therapeutic effect and lower maim rate.关键词：迁延性昏迷;综合治疗;电针结合西药783|0|0更新时间：2023-08-11
- 摘要：目的 :观察针刺对Ⅱ型糖尿病患者血清生长激素 ( growthhormone,GH)的影响 ,以探讨其作用机理。方法 :采用放射免疫法 (RIA)测定单纯针刺组 ( 1 8例 )、针药联合组 ( 52例 )及单纯药物组 ( 50例 )治疗前后的GH含量 ,并进行组间统计学处理。结果 :针药联合治疗与单纯针刺可明显降低患者的GH浓度 (P <0 .0 1 ) ,与单纯药物组比较 ,具有显著性差异 (P <0 .0 5,P <0 .0 1 )。结论 :针刺可通过调节Ⅱ型糖尿病患者生长激素水平 ,协同达到改善高血糖状态的目的Objective: To observe the influence of acupuncture on plasma growth hormone (GH) level in patients with type Ⅱ diabetes for analyzing its action mechanisms. Methods:A total of 120 cases of diabetes patients were randomly divided into simple acupuncture group (n=18 cases), acupuncture+drug group (n=52) and simple drug group (n=50).Plasma GH content was detected with radioimmunoassay.Acupoints of Pishu (BI 20), Shenshu (BL 23),Qihai (CV 6),Quchi (LI 11),Neiguan (PC 6), Zusanli (ST 36), etc, stimulated with uniform reinforcing reducing method first for 15 min and then stimulated electrically with frequency of 80～100 Hz,a tolerable strength and duration of 15 min. The treatment was given once daily,with 10 sessions being a therapeutic course. In simple drug group and acupuncture +drug group,patients were ordered to take Glipizide (2.5～5 mg, 3 time/d), Metformin hydrochloride (0.25～0.5 g,3 times/d),etc.Results:After 2～3 courses of treatment,the levels of GH and fasting blood glucose were reduced obviously in the 3 groups particularly in acupuncture+drug group (P<0.05～ 0.001).Comparison of the difference values (post treatment minus pre treatment) of GH in 3 groups showed that the therapeutic effect of acupuncture +drug group was superior to that of the rest two groups (P<0.05～0.01),and that of simple acupuncture group superior to that of simple drug group (P<0.05). Conclusion: Acupuncture may regulate the level of plasma growth hormone in patients with type Ⅱ diabetes and improve hyperglycemic state.关键词：针刺疗法;Ⅱ型糖尿病 生长激素901|0|1更新时间：2023-08-11
- 摘要：目前研究比较清楚的痛觉调制通路有 :中脑导水管周围灰质 (PAG) -延脑头端腹内侧区 (RVM) -脊髓背角 /三叉神经脊束核通路 ;PAG -伏核 -杏仁核 -缰核 -PAG回路 ;丘脑中央下核 -腹外侧眶皮层 -PAG通路 ;大脑皮层下行抑制通路 :皮层SⅡ -伏核 /缰核 -中缝大核(NRM) -脊髓背角通路 ;皮层SⅡ区 -运动皮层 -丘脑髓板内核群通路 ;皮层SmⅠ区 -尾核 -丘脑束旁核通路 ;下丘脑弓状核 (ARH)痛觉调制通路 :ARH -中缝背核 /蓝斑 /PAG通路 ;ARH -中缝背核 -脊髓背角通路 ;ARH -垂体前叶、中间叶 -丘脑束旁核通路 ;边缘系统 -外侧缰核 -中缝背核 /PAG -NRM/蓝斑 -脊髓背角 /三叉神经脊束核通路 ;痛觉调制的易化通路 :RVM -脊髓背角通路 ;大脑皮层SⅡ -SⅠ通路In the present paper, we review the researches on the neural pathways involving analgesia modulation. Up to now, the clarified pain modulation pathways are 1) the descending inhibitory pathway originating from the brain stem: periaqueductal gray (PAG)-rostral ventromedial medulla(RVM)-spinal dorsal horn/nucleus tractus spinalis nervi trigemini; 2) the mesolibic neuronal loop: PAG-ucleus accumbens-ucleus amygdala-habenula-PAG; 3) thalamic nucleus submedium-ventrolateral orbital cortex-PAG pathway; 4) the descending inhibitory pathways originating from the cerebral cortex: second somatosensory cortex(SⅡ)-accumbens/habenula-nucleus raphe magnus(NRM)-spinal dorsal horn pathway, SⅡ-motor cortex-thalamic intralaminar nuclei pathway and sensorimortor area Ⅰ of the cerebral cortex-caudate nucleus-parafascicular nucleus pathway; 5) the neural pathways emanating from arcuate nucleus of hypothalamus (ARH): ARH-dorsal raphe nucleus/locus coeruleus/PAG pathway, ARH-dorsal raphe nucleus-spinal dorsal horn pathway, ARH-anterior and medial pituitary-parafascicular nucleus pathway; 6) the limbic system-lateral habenula-dorsal raphe nucleus/PAG-NRM/locus coeruleus-spinal dorsal horn/nucleus tractus spinalis nervi trigemini pathways; and 7) the facilitatory neural pathways for analgesia modulation : RVM-spinal dorsal horn pathway and SⅡ-SI somatosensory cortex pathway.关键词：镇痛;通路;研究进展998|0|4更新时间：2023-08-11
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