最新刊期
2002年第2期
- 摘要：目的 :脑室注射γ 氨基丁酸 (GABA)A受体的拮抗剂荷包牡丹碱 (Bic)未能阻断针刺镇痛效应 ,微电泳导入Bic部分阻断电针抑制脊髓背角伤害性反应 ,说明GABA可通过激活A受体参与针刺镇痛中脊髓节段性抑制。本文进一步探讨了激活GABAB 受体在针刺镇痛中的作用。方法 :以辐射热照射大鼠尾部引起甩尾反射潜伏期作为痛阈的指标 ,以针刺“次”穴后痛阈最大变化百分率判断镇痛效应 ,观察脑室注射 (icv) 5μL或蛛网膜下腔 (ith)注射 1 0 μLGABAB 受体的拮抗剂CGP 55845对针刺镇痛效应的影响。每组 6～ 8例。结果 :icvGABA( 1 2 5μg、2 50 μg、50 0 μg)或GABAB 受体激动剂苯氯丁氨酸 (Baclofen ,2 5ng、2 50ng、2 50 0ng)可产生剂量依赖的镇痛效应。icvCGP 55845( 5ng、50ng)可大部分阻断GABA和Baclofen的镇痛效应。针刺双侧“次”穴( 50Hz,1～ 2mA) 1 0min ,痛阈提高到针前值的 ( 1 42 .5± 2 .1 ) % ,镇痛效应显著。针前icvCGP55845针后痛阈分别提高到 ( 1 1 1 .2± 1 .2 ) %和 ( 1 1 2 .1± 1 .1 ) % ,阻断率分别为 73 .7%和 71 .6% ,和事先icv生理盐水 ( 1 43 .7± 2 .0 ) %相比 ,阻断效应明显。若针刺前ithCGP 55845( 50ng、50 0ng) ,也能明显阻断针刺镇痛效应。结论 :脑内注射GABA或Baclofen可通过激?Objective: In this paper, the effects of intra-cerebroventricular and intrathecal injection (icv and ith) of CGP 55845, a potent and selective antagonist against GABA B receptor, on acupuncture analgesia were investigated. Methods: Female Wistar rats were used in the present study. Before experiments, under 10% urethane (1 g/kg, i.p.) anesthesia, a stainless steel cannula was implanted into the left lateral ventricle (B:-1.0～1.2,L:1.5～2.0,H:3.0) according to the "Rat Brain's Sterotaxic Atlas" and fixed with zinc phosphate cement for i.c.v injection. Similarly, a PE-10 polyethylene duct (external diameter 0.61 mm) was inserted into the spinal subarachnoid space till the lumbar enlargement in line with Yaksh and Rudy's method . Radiation heat stimulation was applied to the rat tail and the pain threshold (PT) was detected by tail flick latency tests. Bilateral "Ciliao" (BL 32) were punctured and stimulated electrically with WQ-10 Electroacupuncture Anesthesia Apparatus (50 Hz, 1～2 mA, continuous waves and duration of 10 min). There are 6～8 rats in each group. Results: ① After icv GABA ( 125, 250, 500 μg/5 μL) or baclofen (Bac), an agonist of GABA B receptor at dose of 0.025, 0.25. 2.5 μg/5 μL, the pain threshold (PT) values were increased significantly, presenting a dose-dependent effect, which was markedly blocked by pretreatment of icv CGP 55845 (5, 50 ng). It indicates that icv GABA and baclofen could produce analgesic effects mediated by activation of GABA B receptors. ② After electroacupuncture (EA) at bilateral "Ciliao" (BL 32), PT were raised to (142.5±2.1) % and (143.7±2.0)% without and with pretreatment of normal saline. When pretreated with icv CGP 55845 at dose of 5 and 50 ng /5 μL, acupuncture analgesic effects were significantly blocked by 73.7% and 71.6% ; PT raised to (111.2±1.2)% and (112.1±1.1)%. It indicates that GABA in brain may be involved in acupuncture analgesia mediated mainly by activation of GABA B receptors. ③ When pretreated with ith saline and after EA of bilatreral "Ciliao"(BL 32), PT were raised to (138.2±1.6)%. When pretreated with ith CGP 55845 at dose of 50 and 500 ng /10 μL, PT were raised to (119.0±1.0)% and (109.1±1.9)% respectively, indicating reduce of acupuncture analgesia by 52.6% and 76.7%. It means that at the spinal level, GABA may also be involved in acupuncture analgesia. Conclusion: Microinjection of GABA or Baclofen in the brain may generate dose-dependant analgesic effect via activation of GABA B receptor, and GABA B receptors in the brain and GABA A (our past results) and B receptors in the spinal cord are involved in acupuncture anesthesia.关键词：GABAB受体激动剂;Baclofen;GABAB受体拮抗剂;CGP55845;针刺镇痛1046|0|6更新时间：2023-08-11
- 摘要：目的 :观察苍白球在电针及兴奋尾壳核对脚内核自发放电影响中的作用。方法 :实验在Wistar大鼠上进行 ,采用玻璃微电极细胞外记录的方法 ,观察毁损苍白球 (GP)前后 ,电针“阳陵泉”和“环跳”穴 (强度 2mA ,频率为 2Hz与 1 0 0Hz交替的疏密波 ,持续 5min)及兴奋尾壳核 (微量注射 0 .5mol/L谷氨酸 3 μL)对脚内核 (EP)神经元自发放电的影响。结果 :电针对EP神经元自发放电似有兴奋作用 ,而兴奋尾壳核对其影响不大。毁损GP后电针对EP神经元自发放电的兴奋作用略有增加 ,兴奋尾壳核对EP神经元的自发放电产生抑制作用。结论 :在正常状态下电针对EP神经元自发放电的兴奋作用部分受到GP的抑制 ,兴奋尾壳核通过GP对EP产生兴奋作用Objective : To investigate the roles of rat's globus pallidus(GP) in the effects of electroacupuncture(EA) and excitation of caudate-putamen nucleus(CPu) on spontaneous discharges of neurons in entopeduncular nucleus(EP). Methods: The experiments were performed on 10% urethane-anesthetized (1 g/kg) Wistar rats. Glass microelectrodes were used for recording the spontaneous discharges of neurons in EP before and after chemical lesion (kainic acid, 10 μg/2 μL)of GP. All animals received EA of "Yanglingquan"(GB 34) and "Huantiao"(GB 30) with stimulating parameters of 2 mA of electric current, 2～100 Hz dense-sparse waves, and duration of 5 min. Excitation of neurons in CPu was achieved through microinjection of glutamate(0.5 M, 3 μL) into the nucleus. Results: It was found that the spontaneous discharges of neurons in EP were increased significantly by EA (6～8 min after EA, P<0.05) but not affected remarkably by exciting Cpu (P>0.05). In rats with lesion of globus pallidus, the spontaneous discharges of neurons in EP were increased slightly by EA and inhibited significantly by exciting Cpu (15 min and 20 min after administration of glutamate in comparison with saline group, P<0.05). Conclusion: Results suggest that the excitatory role of EA on the spontaneous discharges of EP neurons is partially inhibited by GP, and the activating CPu can induce an increase of the spontaneous discharges of EP neurons through GP.关键词：苍白球;脚内核;尾壳核;电针;自发放电921|0|0更新时间：2023-08-11
- 摘要：目的 :探讨电针双侧“合谷”穴区对Wistar大鼠局灶脑缺血 /再灌注时大脑皮质cPKCα蛋白表达的影响。方法 :线栓法闭塞Wistar大鼠大脑中动脉 ,制备局灶脑缺血 /再灌注模型。运用免疫组化法检测电针大鼠双侧“合谷”穴区对局灶脑缺血 /再灌注时大脑皮质cPKCα蛋白表达的影响。结果 :假手术组部分动物大脑皮质cPKCα蛋白弱表达 ,局灶脑缺血 3hr时大脑皮质cPKCα蛋白表达增加 ,但未达显著水平 (P >0 .0 5) ,再灌注 3hr、6hr时大脑皮质cPKCα蛋白表达明显增加(P <0 .0 1 ) ,而电针可以明显减少再灌注 3hr、6hr时大脑皮质cPKCα蛋白表达 (P <0 .0 1 )。结论 :电针“合谷”穴区可下调大脑皮质cPKCα蛋白表达 ,这可能与电针抗局灶脑缺血 /再灌注时脑细胞凋亡有关Objective: To explore the effect of electroacupuncture (EA) of "Hegu" (LI 4) on cPKCα protein expression in cerebral cortex in ischemia-reperfusion Wistar rats. Methods: 42 Wistar rats were randomly and evenly divided into sham-operation (control) group, ischemia(I)-3 hr group,I-3 hr+reperfusion (R)-3 hr group, I-3 hr+R-6 hr group and I-3 hr+EA group, I-3 hr-R-3 hr+EA group, and I-3 hr-R-6 hr+EA group (with 6 rats being in each group). Rat Middle cerebral artery occlusion and reperfusion model was established by intraluminal nylon filament-ball occlusion and withdrawal. Cytoplasm cPKCα protein expression in cerebral cortex was displayed using immunohistochemical staining method. EA (acupuncture + electrical stimulation, 40～60 Hz, 1.5 V and duration of 30 min) was applied to bilateral "Hegu"point(LI 4). Results: In control group, very weak cPKCα protein expression was found in some cerebral cortex and basal ganglion neurons in only 3 rats. In I-3 hr group, cPKCα protein expression increased slightly(P>0.05). While in I-3 hr-R-3 hr group and I-3 hr-R- 6 hr group, cPKCα protein expression increased markedly (P<0.01). Following EA stimulation, cPKCα protein expression in I-3 hr-R-3 hr and I-3 hr-R-6 hr groups reduced significantly in comparison with I-3 hr-R-3 hr and I-3 hr-R-6 hr groups (P<0.01), showing an inhibitory action of EA on cPKCα protein expression. Conclusion: EA of bilateral "Hegu"could reduce cerebral ischemia-reperfusion generated cPKCα protein expression, which may be responsible for acupuncture's antiapoptotic mechanism.关键词：局灶脑缺血/再灌注;cPKCα蛋白表达;电针;“合谷”穴1055|0|0更新时间：2023-08-11
- 摘要：目的 :观察电针“水沟”与“井穴”对全脑缺血大鼠脑细胞内活性钙调素 (CaM )含量的影响。方法 :采用大鼠全脑缺血再灌注模型 ,用磷酸二脂酶法测定电针后正常对照组、模型对照组、电针“水沟”穴组、电针“井穴”组、假手术对照组脑组织活性CaM的含量。结果 :脑缺血后脑组织活性CaM含量明显升高 ,电针刺激“水沟”和“井穴”均可使大鼠缺血区脑组织活性CaM含量明显降低 ,两组无显著性差异。结论 :电针“水沟”与“井穴”均能明显降低活性CaM的含量 ,发挥肯定的脑保护作用Objective: To observe the influence of electroacupuncture(EA) at "Shuigou" (GV 26) and "Jing acupoints" respectively on the content of calmodulin(CaM) activity in rat ischemic cerebral region. Methods: The animal models of the cerebral ischemic with reperfusion were made by electric coagulation of bilateral vertebral arteries and occlusion of the ipsilateral common carotid artery. A total of 45 Wistar rats were randomly divided into five groups: ① normal control group(n=9), ② model control group(n=9), ③ sham-operation group(n=9), ④ EA of "Shuigou" group(2～20 Hz, 20 min, n=9), ⑤ EA of "Jing acupoints" group["Shangyang" (LI 1), "Zhongchong"(PC 9), "Lidui" (ST 45), "Zuqiaoyin" (GB 44), 2～20 Hz, 20 min, n=9]. CaM activity of the ischemic cerebral tissues was measured by using phosphodiesterase method. Results: In model group, CaM activity of the ischemic cerebral region elevated obviously in comparison with normal control group and sham-operation group (P<0.001).Following administration of EA, active CaM contents were significantly lower than that of model group (P<0.001). There was no evident difference between EA of "Shuigou" group and EA of "Jing acupoints" group in CaM contents(P>0.05). But CaM contents in both EA groups were still significantly higher than those of normal control group and sham-operation group(P<0.001, P<0.01). Conclusion: Both EA of "Shuigou" and "Jing acupoints" can reduce ischemia-reperfusion induced increase of active CaM level in the rat.关键词：“水沟”穴;“井穴”;电针刺激;脑缺血;钙调素1146|0|3更新时间：2023-08-11
- 摘要：目的 :研究电针督脉经穴“大椎”、“百会”对局灶性脑缺血大鼠脑源性神经营养因子的影响。方法 :凝闭大鼠一侧大脑中动脉 1 0hr后致局灶性脑缺血 ,采用免疫组化染色S P法进行观察。结果 :电针治疗能增强局灶性脑缺血大鼠脑组织脑源性神经营养因子的表达 ,从而阻止神经细胞内Ca2 +超载 ,稳定细胞内环境。结论 :电针对缺血性脑损伤具有一定的保护作用 ,为临床针灸治疗缺血性脑血管疾病奠定了理论基础。关键词：电针;脑缺血;脑源性神经营养因子1011|0|1更新时间：2023-08-11
- 摘要：目的 :了解电针对面神经逆行轴突转运神经营养因子的影响。方法 :同龄成年健康的新西兰兔 ,均在 3 %的戊巴比妥钠静脉麻醉下 ,分离暴露面神经上颊支 ,在手术放大镜下切断神经 ,用硅胶管将两断端嵌入并缝合固定 ,形成再生室。术后动物随机分三组 ,分别接受1 2 5I BDNF ,1 2 5I NT 3或1 2 5I NT 4再生室内注射 ,1 0 μL/只。每组又随机分为针刺组和对照组。针刺组于术后2hr和 2 6hr各接受 1次电针治疗 ,对照组不作任何处理。术后 4hr、8hr、1 2hr、2 4hr、48hr摘取含面神经核的脑干检测 ,两组对照。结果 :对照组脑干中1 2 5I BDNF ,1 2 5I NT 3 ,或1 2 5I NT 4的γ计数在1 2hr和 2 4hr达到峰值 ,48hr又明显降低 ,不同时段波动很大。而针刺组脑干的1 2 5I BDNF ,1 2 5I NT 3或1 2 5I NT 4γ计数在各个时段始终保持在较高的水平。结论 :电针可促进面神经逆行轴突转运神经营养因子Objective:To observe the effect of electroacupuncture (EA) on neurotrophins responsible for facial nerve regeneration in the rabbit. Methods: Under 3% pentobarbital anesthesia (30 mg/kg), a surgical operation was performed in 180 adult New Zealand rabbits by separating and sectioning the buccal branch of the facial nerve trunk and implanting a silicone tube (forming a regeneration chamber) for placing two cut ends of the nerve. All the animals were treated by injecting 125I-BDNF (brain derived neurotrophic factor), 125I-NT(neurotrophin)-3 and 125I-NT-4 (each one being 10 μL) into the chamber respectively. Rabbits treated with the 3 types of NT were randomized into control and EA groups separately. "Yifeng"(TE 17), "Dicang"(ST 4), "Jiache" (ST 6) and "Hegu" (LI 4) were punctured and stimulated electrically with frequency of 18～20 Hz, strength of 1.5 V and sparse-dense waves for 30 min. 4 hr, 8 hr, 12 hr, 24 hr and 48 hr after operation, each 6 animals of the two groups in every time-course were killed for taking out the brain stem samples. 125I-BDNF, 125I-NT-3 and 125I-NT-4 radioactivity of the brain stem tissues was detected with γ-counter respectively. Results: In control group, 125I-BDNF, 125I-NT-3 and 125I-NT-4 count values peaked from 12 hr to 24 hr following operation, while in treatment group, these neurotrophin values in all the time-courses kept in a higher level. Significant differences were found between EA and control groups in different time-courses in the 3 indexes (P<0.01～0.001). Conclusion: Electroacupuncture may facilitate the retrograde transport of the neurotrophins in the facial nerve, suggesting a promoting action of EA on nerve regeneration.关键词：电针;脑干神经营养因子;逆行轴突转运955|0|2更新时间：2023-08-11
- 摘要：目的 :比较不同频度电针治疗大鼠慢性神经源性痛的疗效。方法 :大鼠L5/L6 脊神经结扎慢性神经源性痛模型 (ChungModel) ,给予不同频度的电针治疗 (每天 1次、2天 1次、3天 1次和 4天 1次 ) ,观察疗效。采用引起缩足的机械刺激阈值 ( 50 % )来评价机械性痛觉超敏 ,用大鼠5min内在 5± 1℃冷板上的抬脚次数来反映冷诱发的持续性疼痛。结果 :随着电针次数的增加 ,3天 1次组、4天 1次组、2天 1次组的镇痛效果均可以得到累加。而在四组不同频度中 ,3天 1次组后续镇痛作用效果最好 ,对机械性痛超敏的抑制至电针后 48hr,对冷诱发的持续性疼痛的抑制持续至电针后 1周。经统计学检验 ,3天 1次组与其他针刺频度组镇痛作用的差异有统计学意义 (P<0 .0 1 ,P <0 .0 0 1 )。结论 :电针频度是影响疗效的重要因素 ,综合累加效应和后续效应 ,3天 1次效果最优Objective: Chronic neuropathic pain syndrome characterized by its long duration, slow recovery and difficulty to control in clinic practice makes it more serious physically and psychologically. The present study aims at investigating the optimal interval parameter of electroacupuncture (EA) therapy. Methods:In 10% chloral hydrate (300 mL/kg) anesthetized female SD rats, L 5/L 6 nerve ligation induced chronic neuropathic pain model (Chung model) was used to assess the effect of EA on neuropathic pain. Mechanical allodynia was assessed by using 50% paw withdrawal threshold and cold-induced ongoing pain was detected by the number of paw lift within 5 minutes when free rat was put on to the 5±1 ℃cold plate. 120 rats were divided into 5 groups: control group and 4 intervals of EA subgroups (one-day, 2-day, 3-day and 4-day subgroups, i.e., treatment was given once everyday, every 2, 3 and 4 days separately, 6 sessions altogether). Bilateral "Jiaji" (EX-B 2, L 5, at the sacro-iliac articulation level, about 0.5 cm to the spinal column) and "Weizhong" (BL 40) were punctured and stimulated electrically (2 Hz, wave width 0.6 ms, 0.5～2 mA and duration 30 min) following performing uniform reinforcing-reducing needling manipulation. Results: 7 days after nerve ligation, 50% paw withdrawal threshold lowered significantly and the number of paw lift within 5 minutes increased pronouncedly on the affected side (P<0.001) in comparison with those of healthy side. Among the 4 intervals, 2-day, 3-day and 4-day subgroups rather than 1-day subgroup had an obvious accumulative analgesic effect. As for the long-lasting analgesic effect, 3-day subgroup was the best. Longer lasting analgesic effect was following this interval choice (48 hours for mechanical allodynia and 10 days for cold-induced ongoing pain). As for the accumulative effect and long-lasting analgesic effect (24 hours for mechanical allodynia and 1 week for cold-induced ongoing pain), 3-day group had done a better effect than the others (P<0.01 or 0.001). Conclusion: Optimal interval is a critical factor for the analgesic effect of EA. The results of the present study indicate that one session of EA treatment every 3 days is the optimal interval.关键词：疼痛/神经源性;针刺镇痛;电针频度1130|0|17更新时间：2023-08-11
- 摘要：目的 :本实验采用小鼠强迫游泳实验的方法 ,观察针刺合用抗抑郁药后小鼠在水中的静止时间 ,以此来分析抑郁程度的改善情况。方法 :实验分下列各组 :①生理盐水组 ;②电针加生理盐水组 ;③药物组 ,分别观察了氯丙咪嗪 (氯米帕明 2 .5mg/kg、5mg/kg、1 0mg/kg、2 0mg/kg、60mg/kg)、芬氟明 ( 1 .5mg/kg、3mg/kg、1 2mg/kg)和吗氯贝胺 ( 1 0mg/kg、2 0mg/kg、40mg/kg)等三种药物的作用 ;④上述药物加电针组。结果 :电针能使小鼠在水中静止的时间显著减少 ,腹腔注射 (ip)上述三种药物也能减少小鼠在水中静止的时间 ,并表现出一定的剂量依赖关系。在电针加药组中 ,分别选用三种药物的小剂量 (抗抑郁效果很弱或几乎无效 )与电针合用。氯丙咪嗪( 2 .5mg/kg)、芬氟明 ( 1 .5mg/kg)与电针合用后 ,小鼠在水中静止时间与单用药物组或单电针组相比显著减少。而吗氯贝胺 ( 1 0mg/kg)与电针合用虽也有这种趋势 ,但统计学上尚未达到显著性差异。结论 :电针具有抗抑郁作用 ,电针合用某些小剂量抗抑郁药后即能进一步加强这种效应Objective: In the present study, the effect of electroacupuncture(EA) combined with drugs on the depression was observed by a forced swimming test. Methods: Male Kunming mice were randomly divided into: ① normal saline(NS, n=10) group, ② NS plus EA group (n=10), ③ drug groups--chlorimipramine(2.5 mg/kg, 5 mg/kg,10 mg/kg,20 mg/kg,60 mg/kg), fenfluramine(1.5 mg/kg,3 mg/kg,12 mg/kg) and moclobemide(10 mg/kg,20 mg/kg,40 mg/kg), ④ drug plus EA groups (chlorimipramine 2.5 mg/kg+EA, fenfluramine 1.5 mg/kg + EA, and moclobemide 10 mg/kg + EA). The number of each one-dosage subgroup of each drug in drug groups and drug plus EA groups was 10. EA (sparse waves 4 Hz, series duration 2.5 sec, dense waves 20 Hz, series duration 5 sec, strength≤1 mA) was applied to "Baihui"(GV 20) and Anmian (EX-HN 16) for 40 min. These 3 types of drugs were given by intraperitoneal injection. Results: In comparison with NS group, the duration of immobility (DI) during 5 min test in EA plus NS group was decreased significantly (P<0.01). Compared with that of NS group, the values of DI in chlorimipramine (2.5～ 60 mg/kg, slight dose-dependent tendency), fenfluramine (3～6 mg/kg, no dose-dependent tendency) and moclobemide (10～40 mg/kg, marked dose-effect dependent relationship, r=0.998) groups were also reduced pronouncedly (P<0.01). When the low dose of chlorimipramine (2.5 mg/kg) and fenfluramine (1.5 mg/kg), which hardly had antidepressant effect, were combined with EA, DI values were decreased significantly more than those of either EA plus NS group or drug groups (P< 0.01). The DI of Moclobemide(10 mg/kg) plus EA was smaller remarkably than that of Moclobemide (10 mg/kg, P<0.01), but had no statistical difference compared with that of EA+NS group (P>0.05). Conclusion: EA has an antidepressant effect and EA plus some antidepressants in low doses can potentiate the action of EA further, suggesting that EA plus antidepressant may be a good way for treating depression.关键词：抑郁症;强迫游泳实验;氯丙咪嗪;芬氟明;吗氯贝胺;电针1094|0|5更新时间：2023-08-11
- 摘要：目的 :本研究采用电生理学与形态学相结合的方法 ,探讨了猫心包经“天泉”穴区与心脏联系的途径。方法 :电生理学部分共用健康猫 3 4只 ,乌拉坦 ( 50 0mg/kg)与氯醛糖 ( 50mg/kg,i.p .)混合麻醉 ,用双极铂金丝电极和针灸针分别记录“天泉”穴区的皮神经放电和肌电。形态学部分 :共用健康猫 1 0只 ,1 %戊巴比妥钠 ( 40mg/kg,i.p .)麻醉 ,在左侧“天泉”穴区皮神经、肌肉和左侧心下神经、星状神经节分别注射PI(PropidiumIodide)和bB(bisBenzimide)。结果 :依次分别切断脊髓T2 ～C7节段的背根腹根后 ,电刺激猫左心下神经诱发“天泉”穴区肌电和肌皮神经放电反应所需阈值逐渐增大 ,尤以T1 、C8和C7为甚 ;左侧“天泉”穴区皮神经、肌肉内和心下神经、星状神经节内分别注射微量PI与bB后 ,单标及双标神经元也以C7、C8和T1 节段背根节内居多 ,其中C8最多。结论 :心包经与心脏之间的相互联系主要发生在C7～T1节段Objective: To explore the connection pathway between "Tianquan" (PC 2) of the Pericardium Meridian and the heart with the combined electrophysiological and morphological techniques. Methods: 34 cats anesthetized with urethane (500 mg/kg) and chloralose (50 mg/kg) were used in the first part of the present study. ① After isolating the left inferior cardiac nerve (LICN) and the left lateral cutaneous nerve (LLCN) and performing a skin flap bath of paraffin-oil, discharges of LCN were fed to VC-10 oscilloscope and histogramer via a bipolar platinum electrode (reference electrode placed beneath the subcutaneous tissues), and recorded by using a X-Y recorder. LICN was stimulated with an electric stimulator. The myoelectrical activities of "Tianquan" (PC 2) and "Tianfu" (LU 3) areas were recorded respectively through two pairs of acupuncture needle electrodes. The reaction threshold was set to that the electric stimulation just could evoke myoelectric signal changes. ② In the second part of this study, ten cats anesthetized with 1% pentobarbital (40 mg/kg) were used. Propidium Iodide (PI, 10 μL) was carefully injected into the sheath of LLCN and the muscles around "Tianquan" (PC 2) and bis Benzimide (bB, 5 μL) injected respectively into and the LICN and the stellate ganglion. Results: Following electric stimulation of LICN, the firing rates of LLCN and myoelectrical activity of"Tianquan" (PC 2) increased pronouncedly in comparison with pre-stimulation(P<0.01); while no significant changes of myoelectric signals of "Tianfu" (LU 3) were found. After cutting off the dorsal and ventral roots of C 7～T 2 separately, the thresholds of the discharges of the LLCN and "Tianquan" (PC 2) area responding to stimulation of the LICN increased gradually, being significant in T 1, C 8 and C 7 in comparison with pre-section of nerves. After microinjection of PI and bB, light microscopic examination showed that the single and the double labeled neurons were mainly located in C 7, C 8 and T 1 dorsal root ganglions with C 8 being the utmost. Conclusion: a) There exists a relatively specific connection between "Tianquan" (PC 2) and the heart; b) C 7～T 1 segments may be responsible for the connection between "Tianquan" (PC 2) of the Pericardium Meridian and the heart.关键词：“天泉”穴区;心脏;心下神经;反应阈值1259|0|6更新时间：2023-08-11
- 摘要：目的 :研究针刺“内关”穴对心肌梗塞后血管生成相关因子bFGF和TGF β1 的干预作用。方法 :采用免疫组化方法检测心肌bFGF和TGF β1 。结果 :结扎冠脉 2hr后即可见到心肌细胞内有bFGF和TGF β1 表达 ,2天时bFGF最少 ;而TGF β1 却增至最多。 1周后bFGF较 2天增多 ,开始在血管周围分布 ,3周时少量bFGF分布于尚未形成完整的血管上 ;而TGF β1 却较 2天时减少 ,3周时少量TGF β1 分布于细胞内。二者之间的变化表现出“拮抗”关系。针刺组bFGF表达较同一时相的模型组增多 ,并且血管生成较快 ,较完整 ;而TGF β1 针刺组却较同一时相的模型组减少。针刺“内关”对bFGF的生成具有促进作用 ,而对TGF β1 的产生却具有抑制作用。结论 :针刺“内关”穴具有调节缺血心肌血管生成相关因子bFGF和TGF β1 生成的作用Objective: To research the effect of acupuncture of "Neiguan" (PC 6) on bFGF and TGF-β 1 (angiogenic related factors) expression in ischemic myocardium in the rat. Methods: 72 Wistar rats were randomized into normal group, sham-operation group, model group, and acupuncture group. The later 2 groups were further divided into 2 hr, 2-day, 1-week, 2-week and 3-week subgroups, with 6 cases in each subgroups and the former two groups. Myocardial ischemia (MI) model was established by occlusion of the anterior descending branch of the left coronary artery. Bilateral "Neiguan" (PC 6) were punctured and stimulated with handle manipulation (1 min) and electroacupuncture apparatus (WQ-10CZ, 40 Hz, positive pulse amplitude 4 V and duration of 5 min). bFGF and TGF-β 1 expression was shown by staining the ischemic myocardium sections by immunohistochemical method and observed under light microscope. Results: The bFGF and TGF-β 1 were not expressed until 2 hours after coronary vessel was ligated. 2 days after MI bFGF expression level was the least, while TGF-β 1 expression peaked. One week after MI, bFGF expression level increased compared with that of 2-day subgroup, and distributed around the capillary vessels, myocardial cellular membrane and cellular plasma. Three weeks after MI,lighter TGF-β 1 expression was found and distributed in myocardial cellular, cellular plasma and the vascular wall, which reduced compared with that at 2 days, while lighter bFGF expression distributed in the blood vessels that had not become matured . The variation between bFGF and TGF-β 1 expression showed an antagonistic effect. In acupuncture subgroups, bFGF expression was much heavier than that of the model group at various same phases,furthermore the formation of blood vessels of acupuncture group was more rapidly and entirely than that of model groups. The TGF-β 1 expression in acupuncture subgroups was opposite to bFGF expression. It displayed that acupuncture of "Neiguan" (PC 6) could promote generation of bFGF and had an inhibitory effect on TGF-β 1. Conclusion: Acupuncture of "Neiguan" (PC 6) may promote formation of newborn blood vessels via modulating expression of angiogenic related factors bFGF and TGF-β 1 in the ischemic myocardium.关键词：针刺;“内关”穴;心肌梗塞;bFGF;TGF-β11031|0|3更新时间：2023-08-11
- 摘要：目的 :探讨腧穴不同组方对急性胃粘膜损伤大鼠胃粘膜形态学的影响。方法 :以乙醇造成大鼠急性胃粘膜损伤后 ,动态观察针刺“足三里”不同组穴对模型胃粘膜组织学及超微结构的影响。结果 :①“足三里”不同穴组对急性胃粘膜损伤均具有保护作用。②在不同穴组中 ,以“足三里” +“内关” +“中脘”组效果最好。结论 :“足三里” +“内关” +“中脘”可考虑做为临床针灸治疗胃病的基本处方Objective: To observe the effect of different prescriptions of acupoints on morphological changes in rats with experimental acute injury of gastric mucosa(AIGM). Methods: 72 Wistar rats anesthetized with pentobarbital (40～50 mg/kg) were randomized into control group (n=6), model group, "Zusanli" group, "Zusanli"+"Neiguan" group, "Zusanli" +"Zhongwan" group and "Zusanli" +"Neiguan" +"Zhongwan" group. The last 5 groups were further divided into immediate subgroups, gastric samples were taken immediately after electroacupuncture (EA) treatment and 8 hr-subgroups (samples were taken 8 hours after EA), with 6 cases being in each subgroup. Acute gastric injury model was established by intragastric perfusion of absolute ethyl alcohol (1 mL). Bilateral "Zusanli" (ST 36), bilateral "Neiguan" (PC 6) and "Zhongwan" (CV 12) were punctured and stimulated electrically with frequency 30 Hz, 10～20 V and duration of 10 min. At the end of each experiment, the rat was killed for taking out the whole stomach that was embedded with paraffin and then sectioned and stained with HE. The sections of the stomach were observed under light microscope and electronic microscope for analyzing changes of the injured gastric mucosa. Results: ① After administration of alcohol, gastric mucosal injury was apparent in every subgroups compared with control group, but the injury degrees in every acupuncture subgroup were significantly lighter than that of model subgroups (P<0.05～0.001). ② Among the different acupoint prescription groups, the injured severity of "Zusanli" +"Neiguan" +"Zhongwan" group was lightest under both light microscope and electronic microscope (no obvious swelling of the mitochondria, regular arrangement of the mitochondria crest and the endoplasmic reticulum without dilation). Conclusion: Acupuncture treatment has a protective action on alcohol induced injury of the gastric mucosa, and the prescription of "Zusanli" +"Neiguan" +"Zhongwan" may be used as the basic prescription for treating gastric diseases in clinic.关键词：急性胃粘膜损伤;针刺治疗;组织学超微结构1023|0|0更新时间：2023-08-11
- 摘要：目的 :探讨温经膏穴位贴敷在肺癌治疗中的作用机理。方法 :温经膏穴位贴敷于荷瘤小鼠相当于人体的大椎和肺俞穴 ,观察温经膏对细胞形态、原位末端标记 (TUNEL)及 p53基因的影响。结果 :温经膏贴敷组细胞凋亡指数与CTX组比较无显著性差异 (P >0 .0 5) ,与荷瘤组比较有显著性差异 (P <0 .0 5) ;温经膏贴敷组和CTX组均无p53基因阳性表达及突变 ,贴敷对照组和荷瘤组有p53基因阳性表达及突变。结论 :温经膏能诱导肿瘤细胞凋亡 ,可能有恢复抑癌基因 p53功能的作用Objective: To study mechanisms of acupoint application of "Wenjing" Plaster (温经膏,"Wenjinggao")in treatment of lung cancer. Methods: 40 tumor-bearing mice were randomly and evenly divided into "Wenjinggao" composed of Chansu (蟾酥,Venenum Bufonis), Caowu (草乌,Radix Aconiti Kusnezoffii), Baijiezi (白芥子,Semen Sinapis Albae), Bingpian (冰片,Borneolum), etc group, common plaster control group, cytoxan (CTX) group and cancer model group. Common plaster (composed of vehicle) and "Wenjinggao" were respectively applied to "Dazhui" (GV 14) and "Feishu" (BL 13) for 2 hours every day, beginning from the second day on after inoculation of tumor that was performed by subcutaneous injection (right subaxillary) of cancer cell suspension (S180, 0.2 mL/mouse) to establish cancer-bearing model. In the mice of CTX group, CTX (0.4 mg/case) was given (i.p.) to the mice from the 3rd day on after the inoculation. Ten days later, the mice were killed for taking out the tumor tissues which were sectioned to be observed under light microscope and transmission electron microscope. Apoptosis was assayed by using TdT-mediated dUTP notch end labeling (TUNEL) technique and p53 gene expression was detected with polymerase chain reaction restriction fragment length polymorphismin method. Results: In "Wenjinggao" group, electron microscopic results showed a loose arrangement of the cells, sparse cytoplasm, severe margination of the nuclear chromatin with some chromatinorrhexis (apoptosis), while in model group, no apparent apoptosis phenomenon (dying cells and died corpuscles) mentioned above was found. TUNEL results displayed that the apoptosis indexes of "Wenjinggao" group, CTX group and model group were 3.06%± 1.31%, 3.22%±1.78% and 0.22%±0.09% respectively, no significant difference was found between the former two groups (P>0.05), while there was a significant difference between "Wenjinggao" group and model group in the apoptosis indexes(P<0.05). In "Wenjinggao" group and CTX group, no positive p53 expression strip (117 bp) and mutation (the 248th and 249th codons) were found, while in control group and model group, these positive strip and mutation existed clearly. Conclusion: "Wenjinggao" can evoke apoptosis and renew functions of p53 (cancer-inhibitory gene).关键词：穴位贴敷;细胞凋亡;p53基因;温经膏;肺癌1040|0|2更新时间：2023-08-11
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- 摘要：目的 :明确肾上腺切除大鼠哮喘模型注射针刺治疗后的血清对高嗜酸粒细胞血症大鼠外周血嗜酸粒细胞数目的影响 ,为证明该种针刺血清中存在影响嗜酸粒细胞数目的非皮质激素类活性因子提供实验依据。方法 :将稀释后的针刺血清以 2 .5mL/kg经尾静脉注入模型大鼠 ,连续3天 ,同时连续 1 0天测定该种针刺血清对高嗜酸粒细胞血症大鼠外周血嗜酸粒细胞计数的影响。结果 :与空白血清和普通哮喘针刺血清相比较 ,该种针刺血清在尾静脉注射后第 3～ 1 0天能明显降低高嗜酸粒细胞血症大鼠的外周血嗜酸粒细胞计数 (P =0 .0 1 2 ,0 .0 0 0 ,0 .0 0 0 ,0 .0 0 0 ,0 .0 0 0 ,0 .0 0 0 ,0 .0 0 0 ,0 .0 0 0 )。结论 :肾上腺切除大鼠哮喘模型注射针刺治疗后的血清能明显降低高嗜酸粒细胞血症大鼠外周血嗜酸粒细胞数目 ,提示该针刺血清中存在影响嗜酸粒细胞数目的非皮质激素类活性因子Objective: To investigate whether the serum ("acupuncture serum") collected from adrenalectomized asthma rats which treated with acupuncture has effects on the eosinophil counts in rats with eosinophilia, in order to confirm the existence of nonadrenocortical steroids factors in the "acupuncture serum". Methods: SD rats with eosinophilia (asthma model) were randomly divided into control serum group, common asthma acupuncture serum group and adrenalectomy (ADX) asthma acupuncture group (asthma rats with concomitant adrenalectomy). "Feishu"(BL 13), "Dazhui"(GV 14) and "Fengmen"(BL 12) were punctured. Changes of eosinophils in the peripheral blood in rats with eosinophilia were observed before and after intravenous (tail vein) injection of the "acupuncture serum"(serum∶normal saline = 1∶20, 2.5 mL/kg, from the first day of the model establishment on, continuously for 3 days). The rat's tail blood (20 μL) was collected to be mixed with acidophil granulocyte staining fluid (eosin∶acetone =9∶1) for observing changes of acidophil granulocytes under light microscope.Results: ① Following intravenous injection of control serum, the eosinophil counts in the peripheral blood increased significantly on the 1st, 2nd and 4th day of model establishment (P=0.000, 0.001 and 0.04), but decreased strikingly on the 8th, 9th and 10th day (P=0.024, 0.005 and 0.001) compared with those of model group. ② After i.v. of common asthma acupuncture serum, the eosinophil counts also increased significantly on the 1st, 2nd and 3rd day of model establishment (P=0.000, 0.000 and 0.005), and decreased strikingly on the 7th, 9th and 10th day (P=0.000, 0.000 and 0.004)in comparison with those of model group. ③ Following i.v. of ADX asthma"acupuncture serum", the eosinophil counts decreased significantly from the 3rd day on compared with those of model group(P=0.012, 0.000, 0.000, 0.000, 0.000, 0.000, 0.000 and 0.000). Conclusion: In eosinophilia rats, intravenous injection of "acupuncture serum"has an apparent influence on eosinophil counts. The results indicate that the "acupuncture serum" contains nonadrenocortical steroids factors.关键词：针刺血清;高嗜酸粒细胞血症大鼠;嗜酸粒细胞967|0|5更新时间：2023-08-11
- 摘要：目的 :探讨家兔循经组织温度变化与降钙素基因相关肽 (CGRP)和血管紧张素Ⅱ (AⅡ )含量的关系。方法 :运用红外热像技术 ,选取艾灸后家兔循经出现温度较高部位组织进行分析 ,用放射免疫方法测定外周组织CGRP及AⅡ含量变化。结果 :①循经组织温度较高部位CGRP含量与非高温区比较明显升高 (P <0 .0 0 1 ) ;②循经组织温度较高部位AⅡ含量与非高温区比较无明显改变 (P >0 .0 5)。结论 :艾灸后 ,循经组织温度变化可能与外周组织CGRP功能活动有关Objective: To observe the correlation between temperature changes and CGRP and AⅡ contents in the tissues along the meridians in the rabbit. Methods: Domestic rabbits were used in the present study. After removing the hair On the bilateral hind-limbs, the local body surface temperature was detected before and after moxibustion of "Housanli" (for 15 min) using a infra-red thermography. The local skin, subcutaneous connective tissues and muscles where higher temperature band appeared along the meridian were taken for determining the contents of CGRP (calcitonin gene related peptide) and AⅡ (angiotensin Ⅱ) with radioimmunoassay. Results: The results showed that the content of CGRP in the higher temperature area tissues (14.9±9.11 pg/mg) was apparently higher than that (4.37±4.53 pg/mg)in the lower temperature area tissues (P<0.001); while no significant difference was found between higher and lower temperature area tissues in AⅡ contents (8.97±5.39 pg/mg vs 9.48±6.53 pg/mg,P>0.05). Conclusion: The results suggest that moxibustion induced increase of body surface temperature along the meridian is probably related to the release of CGRP in the peripheral tissue.关键词：循经组织;温度;CGRP;AⅡ;艾灸1072|0|3更新时间：2023-08-11
- 摘要：目的 :研究肩井穴的解剖结构和针刺安全深度。方法 :随机抽样取 57具较新鲜的成年人尸体 ,其中男尸 2 4具 ,女尸 3 3具 ,采用解剖断面法和解剖层次法进行研究。结果 :向下直刺的解剖结构依次是 :皮肤、浅筋膜、深筋膜、斜方肌、肩胛提肌外侧、前锯肌、肋间外肌、肋间内肌、壁胸膜。向下直刺的平均危险深度是 55.96mm。结论 :为了安全 ,建议向下直刺的深度应控制在3 9 .1 7mm之内Objective: To probe into the anatomic structure and safe needling depth of Jianjing (GB 21). Methods: Fifty-seven fresher adult corpses were randomly selected. Among them, 24 were male and 33 female. Anatomic sagittal sectional and lamelled methods were used to study the anatomic structure and suitable needle-insertion depth. Results:Anatomic structures of the perpendicular sections in order are skin, superficial fascia, deep fascia, trapezius muscle, lateral part of the levator muscle of scapula, serratus anterior muscle, external intercostal muscle, internal intercostal muscle and parietal pleura. If the acupuncture needle tip penetrates the parietal pleura, it will puncture the lung to induce pneumatothorax. When Jianjing (GB 21) punctured, the acupuncture needle should be controlled to advance straight downward, avoiding advancing obliquely toward the medial side of the chest. Conclusion:The mean dangerous depth for perpendicular needling insertion downward is 55.96 mm, and for safety, it is proposed that the depth for perpendicular needle-insertion is within 39.17 mm in patient with medium build.关键词：肩井穴/解剖学和组织学;针刺深度;针刺方向1032|0|4更新时间：2023-08-11
- 摘要：目的 :观察针刺对突发性耳聋患者血液流变学和细胞流变学的影响 ,探讨针刺治疗突发性耳聋的作用机理。方法 :采用毫针平补平泻手法 ,针刺耳门、听宫、听会、翳风、中渚、三阴交、太溪 ,每天 1次 ,每次 3 0min ,6天为 1疗程。 4个疗程后采用自身前后对照的方法 ,比较针刺前后的血液流变、细胞流变学检测数据。结果 :突发性耳聋患者血液流变各项指标、细胞流变学红细胞聚集指数均明显高于正常人群参考值 ,红细胞变形指数较正常值低 ,经针刺后全血高切粘度、全血低切粘度、纤维蛋白元含量、红细胞压积及红细胞聚集指数均较针刺前下降明显 (P <0 .0 5) ,红细胞变形能力明显提高 (P <0 .0 5)。结论 :针刺可以降低突发性耳聋患者的高粘血状态 ,提高红细胞的变形能力。提示降低血粘度可能是针刺治疗突发性耳聋的机理之一Objective: To observe the effect of acupuncture on hemorheology and cellular rheology for exploring mechanisms of acupuncture in the treatment of sudden deafness. Methods: A total of 50 deafness inpatients and outpatients were observed in this paper. Ermen (TE 21), Tinggong (SI 19), Tinghui (GB 2), Yifeng (TE 17), Zhongzhu (KI 15), Sanyinjiao (SP 6) and Taixi (KI 3) were punctured, one time a day, with 6 days being a course of treatment. The interval among the therapeutic courses was one day, and the acupuncture treatment was conducted continuously for 4 courses. Before and after acupuncture treatment, blood samples were collected for detecting changes of whole blood high shear visicosity (WBHSV), whole blood low shear visicosity (WBLSV), plasma visicosity (PV), blood cell volume (BCV), fibrinogen (FG), hemagglutination index (HGI) and erythrocyte distortion index (EDI).Results: Before treatment, WBHSV, WBLSV, PV, BCV, FG and HGI values were all obviously higher than those of normal values (P<0.05), after treatment, these values reduced considerably (P<0.05); while EDI of pre-treatment was lower than normal value, after treatment, it increased significantly (P<0.05). It suggests that in deafness patients, acupuncture can apparently improve hemorheology and cellular rheology. Conclusion: Acupuncture can correct abnormal changes of hemorheology and cellular rheology, and improve blood circulation.关键词：血液流变;细胞流变;突发性耳聋;针刺953|0|3更新时间：2023-08-11
- 摘要：互动式针刺法是作者根据自己和同道的临床实践总结出的一种新的治疗方法。本文进一步明确了互动式针刺法的概念 ,初步完善了该法的操作、适应范围 ,提出了该法注重患者守神的论点 ,并将针刺治疗与康复医学、运动医学、身心医学等结合 ,提高了针灸的临床疗效Complementary dynamic acupuncture therapy (CDAT) is a new method founded on the basis of clinical experience. In the present paper, the author retrieves documents about the clinical application of CDTA from 1979 to 2001, defines its concept (i.e., according to the principles of the internal factors being the foundation of changes of every thing, and the external factors being conditions; and the theory of traditional Chinese medicine in entirety concept and treatment being given in the light of syndrome differentiation, when performing acupuncture treatment, ask the patient to conduct active movement of the affected part of the body,or mental activity about movement of the affected limbs, for mobilizing patient's self regulative capability), preliminarily perfects its operation method and clinical indications. Simultaneously, the author of the present paper puts stress on the viewpoint of paying close attention to the patient's mind-keeping, and advocates combination of CDTA with rehabilitation medicine, sports medicine and psychosomatic medicine is the best way for raising the therapeutic effect of acupuncture in clinic.关键词：互动式针刺法;临床适应证;选穴原则;操作方法746|0|15更新时间：2023-08-11
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