最新刊期
2002年第3期
- 摘要：目的 :观察电针督脉经穴“大椎”、“百会”对大鼠缺血区脑组织神经细胞凋亡及NGLF的影响。方法 :用凝闭一侧大脑中动脉的脑缺血大鼠为动物模型 ,采用TUNEL染色法和免疫组化染色S P法进行观察。结果 :缺血 +电针组中 ,大脑皮层梗塞区内TUNEL染色阳性细胞较缺血组显著减少 (P <0 0 1 ) ,NGFR免疫阳性表达在细胞数量上及强度上也均较缺血组及假手术组明显增强 (P <0 0 1 )。结论 :电针能抑制脑缺血后脑内神经细胞的凋亡 ,可增强局灶性脑缺血大鼠脑组织的NGFR的表达 ,对缺血性脑损伤具有一定的保护作用。为临床针灸治疗缺血性脑血管疾病奠定了理论基础Objective: To observe the effect of electroacupuncture (EA) of "Dazhui"(GV 14) and "Baihui"(GV 20) on apoptosis and nerve growth factor (NGF) expression in rats with cerebral ischemia. Methods: 24 Wistar rats were randomly and evenly divided into sham-operation group, ischemia group and EA+ischemia group. Local brain ischemia was produced by occlusion of the middle cerebral artery (MCAo). "Dazhui"(GV 14) and "Baihui"(GV 20) were punctured with filiform needles and stimulated electrically with an EA therapeutic apparatus (5～10 Hz, dense-sparse waves, 3～5 V and stimulating duration of 30 min). The rat's cerebral tissues of various groups were cut into sections to be stained with immunohistochemical method. The TUNEL method was used to display apoptosis state and S-P method employed to show NGF expression. Results: TUNEL staining showed that the number of the died neurocytes in sham-operation group, ischemia group and ischemia+EA group were 0, 676±12 and 326±15 respectively, suggesting that EA could evidently suppress apoptosis of the ischemic cerebral tissues. S-P staining method showed that the number of NGF receptor immunoreaction positive cells of sham-operation group, ischemia group and ischemia+EA group were 2.0±0.3, 6.0±0.3 and 35.0±2.0 separately. In addition, the density of the immunoreaction positive cell staining was also higher in EA+ischemia group. It suggested that EA could remarkably potentiate NGFR expression in the ischemic cerebral tissues (P<0.01). Conclusion: EA can relieve apoptosis of the ischemic cerebral tissues and strengthen NGF expression, suggesting that EA has a certain protective action on ischemic cerebral injury through up-regulation of the expression of NGF.关键词：电针;细胞调亡;神经生长因子;神经生长因子受体1137|0|3更新时间：2023-08-11
- 摘要：目的 :探讨头穴透刺对急性脑梗塞大鼠脑组织中β EP( β Endorphin ,β内啡肽 )与cAMP(cyclicAMP ,环磷腺苷 )含量变化的相关性。方法 :用线栓法直接阻断大鼠大脑中动脉制作急性脑梗塞模型 ,采用放免技术 ,检测头穴透刺对急性脑梗塞大鼠脑组织中 β EP与cAMP含量的影响。结果 :头穴透刺后可使大鼠脑组织中cAMP的含量明显升高 ,使β EP含量明显降低而趋于正常值 ,两者之间呈显著负相关。结论 :头穴透刺可以调节脑组织中 β EP与cAMP的含量 ,改善梗死脑组织供血 ,这可能是针灸发挥治疗作用的重要机制之一Objective: To observe the effect of scalp-acupuncture on cerebral β-EP and cAMP contents and to analyze the correlation between changes of β-EP and cAMP in rats with acute cerebral infarction. Methods: 90 SD rats were randomly divided into control group (n=10), model group (n=40) and scalp-acupuncture group (n=40), those of the later two groups were respectively divided into 4 subgroups further, with 10 cases in every subgroup. Penetration needling from "Baihui" (GV 20) to "Qianding" (GV 21) and from "Shuaigu" (GB 8) to "Xuanli" (GB 6) was performed using filiform needles and the scalp-acupoints were stimulated with G6805 Electroacupuncture Therapeutic Apparatus (1 mA, 30 Hz and duration of 30 min), once after waking up, twice daily afterwards. Acute cerebral infarction model was produced by blocking the blood flow of the middle cerebral artery with a cauterized global nylon thread end (0.25 mm in diameter). The contents of β-EP and cAMP in the brain tissues of rats were assayed with radioimmunoassay (RIA). Results: In model group (including 6 hr, 24 hr, 48 hr and 72 hr subgroups), cAMP contents were markedly lower than that of normal control group; while β-EP contents were significantly higher than that of normal control group (P<0.01). After acupuncture, the content of cAMP increased significantly and that of β-EP lowered evidently in comparison with model group (P<0.05, 0.01), which were close to those of normal control group. There was a significant negative correlation between the contents of β-EP and cAMP (r=-0.8583). Conclusion: Electroacupuncture of scalp-acupoint can adjust the contents of β-EP and cAMP in the brain tissues of rats with acute cerebral infarction, which may contribute to the effect of acupuncture in improving symptoms and signs of cerebral infarction patients.关键词：脑梗塞;头针透刺;电针;β-EP;cAMP997|0|1更新时间：2023-08-11
- 摘要：目的 :探讨戊四唑 (PTZ)点燃型癫痫大鼠脑内氨基酸的变化及电针对其影响。方法 :成年SD大鼠 3 0只随机分为三组 (每组 1 0只 ) :癫痫模型组、电针组、正常对照组。采用氨基酸自动分析仪测定脑内氨基酸含量。结果 :癫痫模型组脑内兴奋性氨基酸谷氨酸 (Glu)及抑制性氨基酸甘氨酸 (Gly)升高明显 (P <0 .0 5) ;而抑制性氨基酸γ 氨基丁酸 (GABA)降低明显 (P <0 .0 1 )。经电针治疗后γ 氨基丁酸 (GABA)、丙氨酸 (Ala)含量明显升高 (P <0 .0 1、P <0 .0 5) ,而Glu降低明显 (P <0 .0 5)。结论 :电针抗癫痫的作用可能与脑内Glu、GABA及Ala的变化有关Objective: To observe the changes of amino acids in the brain of pentylenetetrazole(PTZ) kindled epilepsy rats and to explore the effect of electroacupuncture (EA). Methods: 30 adult SD rats were randomly divided into three groups (10 rats in each group): control group, epilepsy model group and EA group. Epilepsy rat model was established by intraperitoneal injection of PTZ (32 mg/kg), once daily, continuously for 25 days. EA treatment was given to the rat by puncturing and electrically stimulating "Baihui" (GV 20) and "Fengchi" (GB 20) for 30 min (100 Hz, 6 mA), once daily, continuously for 7 days. Contents of aspartic acid (ASP), glutamic acid (Glu), glycine (Gly), γ-aminobutyric acid (GABA) and alanine (Ala) in the brain tissue supernatant were measured with 835-50 Type Amino-acid Automatic Analysis Apparatus. Results: In epilepsy model group, ASP, Glu and Gly contents increased , while GABA and Ala levels decreased in comparison with those of control group. Among them, the increase of Glu (excitatory amino acid) and Gly (inhibitory amino acid glycine) and the decrease of GABA were remarkable (P<0.05～0.01). After EA treatment, the contents of ASP, Glu and Gly presented a decreasing tendency, while those of GABA and Ala increased considerably compared with model group(P<0.01,P<0.05),and the decrease of Glu level was significant (P<0.05).Results indicated that EA could lessen the contents of excitatory amino acids and raise those of some inhibitory amino acids in the brain tissue of epilepsy rats. Conclusion: The effect of EA in treatment of epilepsy is likely related with EA generated changes of excitatory and inhibitory amino acids levels in the brain tissues.关键词：癫痫;氨基酸;电针1075|0|2更新时间：2023-08-11
- 摘要：目的 :本课题前阶段已证明电针能遏制癫痫小发作 ,现拟探查此时脑内一些氨基酸含量的变化。方法 :在戊四氮注射及闪光刺激造成癫痫小发作的大鼠 ,收集脑内一些部位在电针穴位前后的灌流液 ,测定四种氨基酸的含量。结果 :皮层运动区、视区的γ 氨基丁酸、甘氨酸及牛磺酸在电针后显著增加 ,谷氨酸减少。丘脑网状核、黑质网状部的γ 氨基丁酸亦明显增加。结论 :电针促使脑内与抑制有关的氨基酸含量增加 ,而兴奋性氨基酸有减少趋势Objective: To observe changes of amino acid contents in the brain during electroacupuncture (EA) induced suppression of epileptic petit mal. Methods: A total of 5 SD rats with epilepsy were observed in the present paper. Epileptic petit mal was produced by intraperitoneal injection of pentylenetetrazol (PTZ, 35 mg/kg) or PTZ (15 mg/kg) injection plus neon-lamp light stimulation (0.5 Hz, 60 sec) of the eye. "Dazhui"(GV 14) and "Jizhong"(GV 6) were punctured and stimulated electrically for 20 min with G6805 EA Instrument by setting the stimulating parameters as electric current of 5 mA and frequency of 80 Hz. Perfusion fluid samples were collected from the sensory-motor area and visual area of the cerebral cortex, reticular nucleus of the thalamus and reticular part of the substantia nigra via the implanted cannula for detecting glutamate (Glu), γ-aminobrtyric acid (GABA), glycine (Gly) and taurine (Tau) with high pressure liquid chromatography technique. Results: After acupuncture treatment, Glu (excitatory amino acid) contents of the sensory-motor area and visual area of the cerebral cortex decreased slightly but had no significant changes in comparison with pre-treatment. GABA (inhibitory amino acid) contents of the sensory-motor area and visual area, reticular nucleus of the thalamus and reticular part of the substantia nigra, Gly (inhibitory amino acid) contents of the sensory-motor area and visual area all increased considerably compared with pre-EA in rats with epileptic petit mal (P<0.05～0.01). Tau (the excitant of Gly receptor) contents of the sensory-motor area and visual area also increased remarkably (P<0.01). Conclusion: During epileptic petit mal, EA stimulation can lower the excitatory amino acid content to a certain degree and raise the inhibitory amino acid level of the brain significantly, checking epileptic petit mal of the rat关键词：癫痫小发作;电针;脑内氨基酸854|0|2更新时间：2023-08-11
- 摘要：目的 :比较不同经穴电针治疗大鼠慢性神经源性痛的疗效。方法 :选用大鼠L5/L6 脊神经结扎慢性神经源性痛模型 ,给予不同经穴的电针治疗 ,穴位选用“夹脊”(L5)、“环跳”、“委中”、“阳陵泉”和“足三里”。采用引起缩足的机械刺激阈值 ( 50 %缩足阈值 )来评价机械性痛觉超敏 ,用大鼠 5min内在 5± 1℃冷板上的抬脚次数来反映冷诱发的持续性疼痛。分别于电针后即刻、2 4hr、48hr和 72hr检测冷诱发的持续性疼痛 ;电针后即刻、电针后 1 2hr、2 4hr和 48hr检测机械性痛觉超敏 ( 50 %缩足阈值 ) ,以观察其疗效。结果 :以上五个穴位单次电针均有较好的镇痛作用。对冷诱发的持续性疼痛的抑制均可持续至电针后 2 4hr,其中“委中”对冷诱发的持续性疼痛的抑制作用可持续至电针后 48hr;对机械性痛觉超敏的镇痛作用即刻效果较好 ,电针后 1 2hr消失。五个穴位镇痛强度之间在统计学上无显著差异。结论 :临床经验提示穴位选择是影响针刺镇痛效果的重要因素之一。在本实验条件下 ,“委中”穴电针后的镇痛作用持续时间最长 ,可以认为效果最优Objective: The present study is aimed at comparing the analgesic effects of electroacupuncture (EA) at different acupoints in neuropathic pain rats. Methods: L 5/L 6 nerve was ligated with surgical suture thread (gauge-6) to establish neuropathic pain model (Chung method). SD rats were randomly divided into 5 groups, namely "Jiaji"(EX-B 2), "Huantiao"(GB 30), "Weizhong"(BL 40), "Yanglingquan"(GB 34) and "Zusanli"(ST 36) acupoint groups, with 11 cases being in each group. These acupoints were punctured with acupuncture needles and stimulated electrically with HANS (wave duration 0.6 ms, 2 Hz, 0.5～2 mA) for 30 min. Mechanical allodynia was assessed by 50% paw withdrawal threshold with von Frey filaments and cold-induced ongoing pain was detected by the number of paw lifts within 5 min when the rat was put on to the 5±1 ℃ cold plate freely. Fifty percent paw withdrawal threshold was assessed immediately following EA, 12 hrs and 24 hrs following EA treatment, and cold-induced ongoing pain was also assessed just after EA, 24 hrs, 48 hrs and 72 hrs following EA treatment. Results: After the EA treatment, all the 5 acupoints had instant analgesic effects on neuropathic pain (P<0.01, 0.001) and the analgesic effect could maintain 24 hrs. There was no significant difference among the 5 acupoint groups concerning the long-lasting analgesic effect on 50% paw withdraw threshold and cold-induced ongoing pain. Only "Weizhong"(BL 40) produced longer long-lasting analgesic effect on cold-induced ongoing pain (48 hrs following EA treatment). The immediate effect of EA on mechanical allodynia was better, lasting about 12 hrs. Conclusion: Results of the present study demonstrate that EA treatment has a remarkable analgesic effect, while "Weizhong"(BL 40) seems to be better than the other 4 acupoints under our experimental conditions.关键词：神经源性痛;针刺镇痛;穴位1185|0|3更新时间：2023-08-11
- 摘要：目的 :观察针刺麻醉复合丹参对心肌缺血再灌注损伤的保护作用及维持心肌线粒体的功能。方法 :1 8只中国大耳白兔随机等分为对照组、针刺麻醉组、针刺麻醉复合丹参组 ,在急性心肌缺血再灌注模型上观察冠状动脉左前降支结扎前即刻、灌注前即刻、灌注 2hr三个时间点MAP、HR、ECG ,测定血浆IL 8、乳酸浓度 ,测定心肌缺血再灌注区、非缺血再灌注区线粒体的99mTc MIBI摄取率。结果 :血IL 8、乳酸浓度对照组、针刺组、针刺麻醉复合丹参组逐步减少 ,心肌缺血再灌注区线粒体99mTc MIBI摄取率逐步增高 ,血乳酸值和线粒体99mTc MIBI摄取率有强负相关关系。结论 :IL 8参与中性粒细胞介导的心肌再灌注损伤 ,针刺麻醉、针刺麻醉复合丹参能减少IL 8的产生及减弱其作用效应。针刺麻醉能减轻心肌再灌注损伤 ,维持心肌线粒体的功能 ,丹参对针刺麻醉的心肌保护效应有增强作用Objective: This study is designed to investigate whether acupuncture anesthesia combined with intravenous administration of Danshen (Salviae Miltiorrhizae Radix) injection can protect the ischemic myocardium. Methods: 18 rabbits were randomly and equally assigned to control group, acupuncture anesthesia (AA) group and AA plus Danshen (AAD) group. The animals were subjected to acute myocardial ischemia-reperfusion (AMIR) injury by ligating and then releasing the left anterior descending branch of the coronary artery. Electroacupuncture (5 Hz, 30 min) was applied to bilateral "Neiguan"(PC 6), "Yunmen"(LU 2) and "Lieque"(LU 7). MAP,HR and ECG were monitored and recorded during experiments, and plasma interleuin(IL)-8 and lactic acid contents in blood samples taken from the right atrium assayed before AMI, after AMI (before reperfusion) and 2 hr after AMIR respectively. Myocardial mitochondria uptake of 99m Tc-MIBI (Tc 99m sestamibi) in the ischemic zone and in the non-ischemic zone of the heart was measured with radioimmunoassay. Results: Plasma IL-8 contents of the 3 groups increased after AMI and 2 hr after AMIR in comparison with pre-AMI. Comparison among the 3 groups showed that IL-8 levels of AMI and 2 hr after AMIR of AA and AAD groups were significantly lower than those of AMI and 2 hr after AMIR in control group (P<0.05). Plasma and lactic acid levels of the 3 groups presented a similar tendency compared with plasma IL-8. In control group the myocardial mitochondria uptake of 99m Tc -MIBI in the ischemic zone was lower than that of non-ischemic region, while in AA and AAD groups, it was opposite. Comparison among the 3 groups showed that the uptake of 99m Tc-MIBI of AA and AAD groups in the ischemic region was significantly higher than that of control group (P< 0.05), but that of AA and AAD groups was lower than that of control group (P<0.05 in AAD group). There was a negative correlation between plasma lactic acid content and myocardial mitochondria uptake of 99m Tc-MIBI (r=-0.791). Conclusion: Interleukin-8 was involved in the myocardial ischemia-reperfusion injury mediated by neutrophils. AA and AA plus Danshen could reduce IL-8 mediated myocardial ischemia-reperfusion injury, maintain the functions of myocardial mitochondria; and combination of acupuncture anesthesia and Danshen had a synergistic protective effect on ischemic myocardium.关键词：心肌再灌注损伤;针刺麻醉;丹参;线粒体;白细胞介素-8;99mTc-MIBI1137|0|2更新时间：2023-08-11
- 摘要：目的 :探讨电针的抗心肌缺血机制 ,进一步研究经脉脏腑相关理论。方法 :采用大剂量异丙肾上腺素 ( 1 0 0mg/kg)腹腔注射建立急性心肌缺血实验模型 ,观察电针“心俞”、“厥阴俞”对血清中肌酸激酶 (CK)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)、血浆中降钙素基因相关肽 (CGRP)和内皮素 (ET)含量的影响。结果 :电针“心俞”、“厥阴俞”能降低血清CK含量 (P <0 .0 5) ,和MDA含量 (P <0 .0 1 ) ,提高血清SOD含量 (P <0 .0 0 1 )和血浆CGRP/ET水平 (P <0 .0 0 1 )。结论 :电针对大鼠急性心肌缺血具有预防和治疗作用 ,可以保护心肌 ,改善心肌缺血损伤 ,进一步证实了经脉脏腑相关理论Objective: To probe into mechanisms of electroacupuncture (EA) in resisting myocardial ischemia (MI) and to study the relationship between "Xinshu"(B 15)and "Jueyinshu"(B 14)acupoints of the Bladder Channel and the heart. Methods: EA (300 μs pulse width; 2～20 Hz; 3.5～5 V) was applied to left "Xinshu"(B 15)and "Jueyinshu"(B 14)for 30 min. 32 Wistar rats were evenly and randomly divided into prevention EA group, EA group, model group and control group. MI was induced by intravenous injection of isoproterenol (ISO, 100 mg/kg). In EA-prevention group, EA was performed only one time 1 hr before injection of ISO. In EA group, EA was preformed at the moment of injection of ISO and given once again 12 hr later. In model group, only ISO was used, and in control group normal saline (5 mL/kg) was injected intravenously. Changes of serum superoxide dismutase(SOD)activity, serum malondialdehyde (MDA) and creatine kinase (CK) contents were assayed using colorimetric method, while plasma calcitonin gene-related peptide (CGRP) and endothelin(ET) contents were detected with radioimmunoassay. Results: After MI, serum CK levels in EA-prevention group and model group increased significantly in comparison with control group(P<0.01～0.001), but there was no significant difference between EA and control group (P>0.05), showing the effect of EA being superior to that of EA-prevention. Following EA, serum SOD in the aforementioned two EA groups increased significantly in comparison with control group (P<0.05～0.01) but no apparent difference was found between model group and control group (P>0.05). Serum MDA level of model group was significantly higher than those of two acupuncture groups and control group (P< 0.01), but there was no significant difference between EA and control group (P>0.05)in spite of being higher in EA group. Plasma CGRP of model group was significantly lower than those of control group (P<0.001), suggesting an increase of CGRP after MI. After EA, CGRP levels in two EA groups increased moderately, being close to that of control group. Following MI, plasma ET elevated significantly in comparison with control group and EA group(P<0.01); but there was no significant difference between EA group and control group (P>0.05), suggesting a suppressive action of EA on ET release. In regard to CGRP/ET , there was no apparent difference between control group and EA group (P>0.05), while there was no significant difference between EA and control group (P> 0.05). Conclusion: EA can effectively inhibit MI-induced increase of serum CK, free radical oxidation and plasma ET levels and raise plasma CGRP content, that may be associated with the good curative and preventive effects of EA on ischemic myocardial injury.关键词：电针;心肌缺血;降钙素基因相关肽;内皮素;超氧化物歧化酶;丙二醛1146|0|3更新时间：2023-08-11
- 摘要：目的 :观察电针对狗胃粘膜血流量的调控作用及其与血浆、胃粘膜组织中内皮素水平变化的关系 ,以探讨电针对胃粘膜保护作用的机制。方法 :将 2 0条狗随机分为四组 :空白对照组、非经非穴组、“上巨虚”组、“足三里”组 (每组 5条 )。采用激光多普勒血流仪监测狗胃粘膜血流量的变化 ,同步测定血浆及胃粘膜组织中内皮素含量 ,并观察其变化规律。结果 :给予电针 3 0min ,停针 3 0min后“足三里”组胃粘膜血流量较针前显著升高 (P <0 .0 1 ) ,血浆及胃粘膜组织中内皮素含量显著下降 (P <0 .0 1 ) ,电针后“上巨虚”组仅血浆内皮素含量较针前下降 (P <0 .0 5) ,而胃粘膜血流量及胃粘膜组织内皮素含量无显著变化 ,其他组各监测指标无显著变化。结论 :电针可使狗胃粘膜血流量增加 ,对胃粘膜具有保护作用 ,这种变化与影响胃粘膜血流量某些脑肠肽的含量改变有关 ,具有一定的穴位特异性Objective: To investigate the regulation action of electroacupuncture(EA)on gastric mucosal blood flow (GMBF) and its relation to the contents of plasma and gastric mucosal ET in dogs. Methods: Twenty mongrel dogs (body weight 15～20 kg) anesthetized with pentobarbital (1 mg/kg) were randomly and equally divided into control group,non-acupoint group,"Shangjuxu" group and "Zusanli" group. "Shangjuxu"(ST 37), "Zusanli"(ST 36) and non-acupoint (the midpoint between the small head of the fibula and the external malleolus, 1 cm to the lateral side of the fibula) were punctured and stimulated with an EA therapeutic apparatus (50 Hz, intermittent waves, 20 V) for 30 min. GMBF (gastric body) was measured by using a laser doppler flowmeter(LDF),and plasma ET contents were detected with radioimmunoassay method. Results: In "Zusanli" group,30 min after EA stimulation and cease of EA, GMBF increased significantly (P<0.05, 0.01); while no significant changes were found after EA in "Shangjuxu", non-acupoint and control groups. Simultaneously, 30 min after cease of EA, marked decrease was observed in plasma ET content (P<0.01) and gastric mucosa ET content (P<0.01) in both "Zusnanli" and "Shangjuxu" groups, whereas no significant changes were observed in control and non-acupoint groups in plasma and gastric mucosal tissue. Conclusion: EA at "Zusanli" (ST 36) can evidently make GMBF increase, suggesting a protective effect of EA on gastric mucous. This effect is probably related to EA-induced changes of plasma and gastric mucosa brain-gut peptide and the effect of EA of different meridian acupoints is of a certain specificity.关键词：电针;胃粘膜血流量;内皮素1037|0|0更新时间：2023-08-11
- 摘要：目的 :探讨麦粒灸对类风湿关节炎大鼠TNF α、IFN γ的影响。方法 :以佐剂性关节炎(AA)作为类风湿关节炎大鼠模型 ,随机分为正常对照组、模型组、麦粒灸治疗组、麦粒灸加去肾上腺组、麦粒灸加假手术组 ,麦粒灸“肾俞”、“足三里” ,检测TNF α、IFN γ含量变化。结果 :正常对照组、模型组、麦粒灸治疗组、麦粒灸加去肾上腺组、麦粒灸加假手术组大鼠血清TNF α水平分别是1 2 .1 3± 9.0 1 pg/mL、46.75± 1 6.77pg/mL、1 3 .2 5± 8.64 pg/mL、3 1 .88± 2 4.3 4pg/mL、6.88±4.1 7pg/mL ;正常对照组、模型组、麦粒灸治疗组、麦粒灸加去肾上腺组、麦粒灸加假手术组大鼠血清IFN γ含量分别为 470 .2 3± 1 0 2 .0 1ng/L、1 45.58± 46.65ng/L、451 .1 4± 98.64ng/L、2 53 .88±75.44ng/L、42 5.97± 1 1 4.1 7ng/L。结论 :麦粒灸下调AA大鼠血清TNF α水平和上调AA大鼠血清IFN γ水平 ,二者均有赖于肾上腺的完整。关键词：类风湿关节炎;细胞免疫;麦粒灸918|0|3更新时间：2023-08-11
- 摘要：目的 :探讨电针对类风湿性关节炎 (RA)大鼠糖皮质激素 (CS)及受体 (GCR)的影响。方法 :采用佐剂性关节炎大鼠 (AA)作为RA动物模型 ,电针“足三里”治疗后 ,放免测定血浆CS、胸腺GCR含量。结果 :模型组大鼠血浆CS明显下降 ,电针后CS水平明显升高 ,二者比较 ,P <0 .0 5,且达到正常水平 ,电针组与正常组比较 ,P >0 .0 5;模型组大鼠胸腺GCRKD值显著低于正常组和空电组 (P <0 .0 1 ) ,其浓度也明显低于正常组、空电组 (P <0 .0 5) ,电针治疗后GCRKD值和浓度水平与模型组相比均有显著提高 (P <0 .0 1 )。结论 :电针能够增加RA大鼠的CS、GCR含量水平Objective: To investigate the effect of electroacupuncture (EA) of "Zusanli"(ST 36) on plasma corticosterone (CS) level and glucocorticosteroid receptor (GCR) affinity and concentration in rheumatoid arthritis(RA) rats. Methods: 40 SD rats were randomly and evenly divided into normal control, blank EA, model and model+EA groups. The rheumatoid arthritis rat model was established by injecting Freund's complete adjunvant(FCA) into the right posterior paw. EA (2～4 V, 2～100 Hz frequency) was applied to bilateral "Zusanli"(ST 36) for 20 min, once daily, with 7 days being a therapeutic course, 3 courses all together. Plasma CS concentration was detected with radioimmunoassay and the dissociation constant (KD) and concentration of GCR in the thymus gland supernatant were calculated and measured. Results: In model group, the pain threshold decreased significantly compared with that of normal control group (P<0.05); while in blank EA and model+EA group, it increased considerably (P<0.01). Simultaneously, the foot volume (reflecting the swelling state) in model group increased on the 7 th day and peaked on the 14 th day after injection of FCA, whereas that of model+EA group decreased continuously, showing a significant swelling-relief effect of EA (P< 0.05, 0.01, vs model group). In model group, plasma CS concentration, KD and GCR concentration declined obviously compared with normal group (P<0.05, 0.01, 0.01), in EA+model group, they were close to those of normal control group, displaying a significant difference between model and EA+model group in CS content and KD and GCR concentration (P<0.05, 0.01, 0.01). It showed that EA could significantly raise plasma CS and GCR affinity and level in the thymus gland, potentiating the cellular immunofunction in RA rats. Conclusion: EA of "Zusanli"(ST 36) can increase RA rat's corticosterone concentration in plasma and glucocorticosteroid receptor levels in plasma and thymus gland to enhance the cellular immune capability.关键词：类风湿关节炎;糖皮质激素;糖皮质激素受体;电针疗法1060|0|3更新时间：2023-08-11
- 摘要：目的 :揭示任脉六穴与胸腺的内在联系 ,探讨针灸对应激状态下小鼠细胞免疫功能的调节作用及机理。方法 :通过长期的捆绑刺激使小鼠处于应激状态 ,分别采用手针和电针刺激小鼠的任脉六穴 ,观察针刺前后NK细胞活性、白细胞介素 2活性的变化。结果 :应激组中NK细胞活性和白细胞介素 2活性均低于对照组 (P <0 .0 5,P <0 .0 1 ) ,而手针组、电针组与应激组相比NK细胞的活性和白细胞介素 2活性均得到提高 (P <0 .0 1 ,P <0 .0 5)。结论 :针刺任脉六穴可以提高应激状态下小鼠的细胞免疫功能 ,其作用机制主要是通过对胸腺的影响 ,进而提高小鼠的NK细胞活性和白细胞介素 2的活性 ,从而显现出针刺的促防卫免疫效应Objective: To observe the effect of acupuncture of 6 acupoints of the Conception Vessel (CV) on the activities of NK cells and interleukin(IL)-2. Methods: NIH mice were randomized into control group (n=15), stress group (n=15), handle-needling group (n=15) and electroacupuncture (EA) group (n=15). "Danzhong"(CV 17), "Yutang"(CV 18), "Zigong"(CV 19), "Huagai"(CV 20), "Xuanji"(CV 21) and "Tiantu"(CV 22) were punctured with acupoint to acupoint penetration method and stimulated for 20 min. The treatment was conducted once daily, with 10 days being a therapeutic course and with the interval between two courses being one week. Following 3 courses of treatment, the mouse was killed for sampling spleen and thymus tissues. NK cell and IL-2 activities in cell suspension of the spleen and thymus were detected. Results: In stress group, both NK cell and IL-2 activities were significantly lower than those of control group, while those of handle-needling and EA groups were apparently higher than those of stress group (P<0.05～ 0.01), suggesting that acupuncture can raise NK cell and IL-2 activities in stress mice. Conclusion: Both handle-needling and electroacupuncture can enhance activities of NK cells and IL-2 in stress mice.关键词：针刺;应激状态;NK细胞与IL-2活性991|0|4更新时间：2023-08-11
- 摘要：目的 :探讨针刺治疗动脉再狭窄的作用机理。方法 :用空气干燥法诱发大鼠颈总动脉再狭窄模型 ,以复方丹参注射液作对照 ,观察了针刺对动脉再狭窄模型大鼠右颈总动脉去内皮损伤的内膜增生及纤溶活性的影响。结果 :针刺能抑制动脉去内皮损伤的内膜增生 ,升高模型大鼠血清中组织型纤溶酶原激活剂 (t PA)的活性 ,降低纤溶酶原激活剂抑制物 (PAI)的活性 ,从而影响动脉再狭窄的病理进程。结论 :针刺对动脉再狭窄具有较好的防治作用Objective: To explore the mechanism of acupuncture in preventing and treating arterial restricture. Methods: Twenty-four SD rats were used in the present study. Rat carotid artery restricture model was established in accordance with Fishman's method. These rats were randomly and evenly divided into control, acupuncture and Danshen (Radix Salviae Miltiorrhizae, 丹参)groups. "Neiguan"(PC 6), "Jueyinshu"(BL 14), "Xinshu"(BL 15), "Danzhong"(CV 17) and "Zusanli"(ST 36) were punctured using filiform needles and stimulated with uniform reinforcing-reducing method. The treatment was conducted once daily beginning from the first 2 days on before operation (establishing model) to the 14th day after operation. The 8 rats of Danshen group were fed with Danshen injectio (1.44g/kg/d) once daily and those of control group fed with normal saline (10 mL/kg/d) once daily. The duration of Danshen and saline treatment was the same to that of acupuncture group. Serum t-PA (histiocytic type plasminogen activator) and PAI (plasminogen activator inhibitor) activities were assayed with enzyme linked immunosorbent assay. Results: Electronic microscopic observation showed that arterial restricture injury included severe platelet adhesion, exfoliation of the vascular endotheliocytes, etc., suggesting success of the model. After treatment, the thickness values of the vascular endangium and the media on the injured side in control, Danshen and acupuncture groups (in each group, the tested arteries=16) were 79.86±7.88 μm and 46.88±4.60 μm; 17.04±1.22 μm and 40.25± 3.0 μm; 17.25±0.93 μm and 39.88±2.50 μm separately, presenting significant differences between Danshen or acupuncture and control groups (P<0.05～0.01). The serum t-PA and PAI and PAI/t-PA in control, Danshen and acupuncture groups were 0.15±0.08(10 3 U/L), 1.74±0.16 (10 3 U/L) and 15.16±2.37; 0.22±0.05(10 3 U/L), 1.19±0.08(10 3 U/L) and 7.6±1.67; 0.23±0.10(10 3 U/L), 1.16±0.12(10 3 U/L) and 6.4±1.59 respectively. There were significant differences between Danshen or acupuncture and control groups (P<0.05～0.01). Conclusion: Both Danshen and acupuncture can lighten vascular injury, raise t-PA activity and reduce PAI activity.关键词：针刺;动脉再狭窄;血管内膜;组织型纤溶酶原激活剂;纤溶酶原激活剂抑制物984|0|0更新时间：2023-08-11
- 摘要：目的 :观察循经温度现象与Na+/K+ ATP酶活性变化及血管调控物质SP功能活动的关系。方法 :①用红外热像技术观察循经出现的温度变化。② )用酶学方法测定Na+/K+ ATP酶活性 ,放射免疫方法测定P物质 (SP )含量。结果 :①左后肢外侧和右后肢内侧循经高温区组织Na+/K+ ATP酶活性均高于对照区组织 (P <0 .0 5、P <0 .0 1 ) ;②左后肢外侧和右后肢内侧循经高温区组织的SP含量较对照区组织有升高趋势 ,但均无统计学差异 (P >0 .5,>0 .1 )。结论 :①家兔循经出现温度较高组织的Na+/K+ ATP酶活性较非高温区组织的代谢活跃 ;②家兔循经高温区组织P物质水平有一定增高 ,但未见统计学意义Objective: To observe the interrelation between moxibustion induced changes of temperature and Na +/K +-ATPase activity as well as SP content in the tissues along the ruuning course of meridians. Methods: Seven healthy mongrel rabbits were used in this study. Before and after moxibustion (5 min) of unilateral "Zusanli" (ST 36) or "Yinlingquan"(SP 9), the cutaneous temperature along the Stomach Meridian was detected with an infra-red thermography. The skin and muscle tissues showing higher temperature along the Stomach Meridian and the Spleen Meridian of the hindlimb part and those of non-higher temperature region (beside the higher-temperature region) were taken respectively under the monitoring of the infra-red thermography. The tissue samples of higher temperature region and non-higher temperature region were taken tobe processed into tissue homogenate, followed by detecting the activity of Na +/K +-ATPase with enzymologic method and the contents of SP(substance P) with radioimmunoassay. Results: ① After moxibustion, the cutaneous temperature increased significantly. μmol Pi/mg prot/hr. The activity values of Na +/K +-ATPase in the higher and non-higher temperature areas were 1.922±0.066 μmol Pi/mg prot/hr and 1.649±0.122 μmol Pi/mg prot/hr at the lateral side of the left hindlimb, and 2.189±0.241 μmol Pi/mg prot/hr and 1.832± 0.132 μmol Pi/mg prot/hr at the medial side of the right hindlimb separately . It shows that the activity of Na +/K +-ATPase in the higher-temperature region is considerably higher than that of the lower temperature area (P<0.05, 0.01); ② The contents of SP in the higher and non-higher temperature areas were 118.92±28.17 pg/mL and 111.39±29.46 pg/mL at the lateral side of the left hindlimb, and 181.06±24.50 pg/mL and 135.74±26.19 pg/mL at the medial side of the right hindlimb separately. It shows that SP contents in the higher-temperature region are higher slightly than those of non-higher temperature region despite no statistical differences between them(P>0.5,0.1). Conclusion: Results suggest that the metabolism of tissues in the higher-temperature region is active and SP may play a minor role in moxibustion-induced higher-temperature phenomenon along the meridian.关键词：温灸;循经温度;Na+/K+-ATP酶;P物质962|0|3更新时间：2023-08-11
- 摘要：目的 :建立穴位断面数字解剖学 ,提高穴位的形态认识。方法 :采用了标本灌注后穴位的断面切割和逐层切割方法。建立穴位断面标本图像库和人体标定穴位后的逐层断面解剖图片库。结果 :获得 43 9个穴位断面标本和图像 2 3 0 0张 ,全身逐层断面图像标本 1 0 58张。结论 :人体穴位标本断面切割是研究和实验数字穴位解剖学的基础 ,切割标本也为穴位的其他相关的形态和机能的系统研究提供了原始的形态图像资料Objective: To study the practicability of the cutting approach of the acupoint with 3-dimensional reconstruction computer software and to provide primary anatomic image data of acupoints. Methods: Four male corpses were used as the samples. According to Chinese United National Criteria for Acupoint Locations formulated in 1990, 157 commonly-used acupoints were located first, followed by arterial infusion of 1% heparin-saline (2000 mL), 5%, 10% and 20% ABS-2-butanone mixed with scarlet dye (repeated the infusion procedures on the 2 nd and 3 rd day with the same 20% fluid). The human corpse was frozen for 7 days under -30 ℃. A hole was made at the orientated acupoint by using a high-speed bit and then filled with plasticine, with the sample being frozen again for 3 days more. The frozen samples were collected to be cased (wooden cases) and frozen again under -30 ℃ for 2 days. Following fixing, the samples were cut below 2 mm thick sections along the sagittal or horizontal section with a high-speed steel plane machine. Following cutting, the located position was restored, photographed and videotaped simultaneously. Results: A total of 1058 photos (2300 pieces of image library) of sectional anatomic specimens of 209 acupoints were collected to be imported into a computer. Our research showed that acupoint is organically composed of many kinds of normal tissues which make up a space construction. It is not easy to comprehend and grasp acupoint's anatomical structure, especially those liable to induce unfavorable results (if a wrong needling operation was adopted) but important, while it is very important for clinical treatment and teaching yet. Gathering of the image data is much useful for acupuncture teaching, clinical treatment, scientific research, self-study and popularization of TCM knowledge. Conclusion: To adopt corpse sample to take anatomical images of various layers and sections of the acupoint and to utilize computer software to perform cutting and reconstruction of the human body can help us observe various structures of the acupoint from different angles.关键词：穴位断面结构;计算机切割和重建方法;图像数据库905|0|8更新时间：2023-08-11
- 摘要：目的 :探讨内皮素在头针治疗中风病中的作用机制。方法 :采用放射免疫技术 ,观测头针对中风病人血浆中内皮素含量的影响。结果 :头针治疗可以使中风病人血浆中过度升高的内皮素含量明显下降。结论 :头针治疗可以通过调节内皮素含量而达到治疗效果Objective: To investigate the mechanism of scalp-acupuncture in treatment of apoplectic patients. Methods: A total of 30 apoplectic inpatients (treatment group, 20 cases of cerebral infarction, 9 cerebral hemorrhage and one mixed type) and 15 normal people (control group) were subjected to this study. Dingnie Qianxiexian (MS 6) and Dingnie Houxiexian (MS 7) were punctured with Gauge-30 filiform needles that were manipulated about 200 times/min for 2～3 min, once again every 5 min, and then retained for 30 min. The treatment was conducted once daily and 6 sessions of treatment formed a therapeutic course. Plasma endothelin (ET) was assayed by using radioimmunoassay. Results: Before the treatment , plasma ET was 117.93±31.80 pg/mL, being significantly higher than normal level (54.80±13.21 pg/mL, P<0.01). After scalp-acupuncture treatment, plasma ET was 70.87±29.49 pg/mL, being significantly lower than that of apoplectic patients before treatment (P<0.01). Conclusion: Scalp-acupuncture treatment induced decrease of plasma ET may be one of the factors for improving clinical symptoms and signs of apoplectic patients.关键词：中风;头针疗法;内皮素948|0|1更新时间：2023-08-11
- 摘要：本文从①循经感传现象的外周和中枢机理 ;②经脉 脏腑相对特异性联系的生理学与形态学基础 ;③经脉循行线上理化特性的观察和机制分析 ;④古代经络文献研究与现代经络研究史等四个方面概述了“九五”期间经络研究的最新进展。这些研究结果揭示 :循经感传与外周神经、骨骼肌链、脊髓运动神经元柱和大脑皮层等结构和有规律性的功能活动密切相关 ;针刺经脉穴位对相应脏腑功能活动的相对特异性作用有其相关的神经生物学物质基础 ;经脉循行线上出现的各种生物物理现象包括红外辐射轨迹、液晶等与机体生物信息的特殊传递活动密不可分 ;经络是指人体体表与体表、体表与内脏远隔部位特定联系的规律 ;目前我国对于经络的研究处于世界领先水平 ,有关经络研究的突破还有很长的路要走In the present paper, the authors review the newest advances (1998～2001) of research on "meridian theory" of traditional Chinese medicine from ① the peripheral and central nervous mechanisms of "acupoint stimulation induced sensations transmitting along meridians (STM)"; ② physiological and morphological basis of the relatively specific relationship between meridians and Zangfu-organs; ③ observation on the biophysical and biochemical characters of meridian running traces and analysis on their related mechanisms; ④ research on the ancient documents about meridian theory and recent history of meridian research. Results reveal that STM phenomenon is closely related to the peripheral nerves, skeleton muscle chains, spinal motor neuron column and cerebral cortex, and their regular functional activities. The relatively specific action of acupuncture of acupoints on the corresponding Zangfu-organ has its neurobiological material basis. Various biophysiological phenomena including infra-red radiation trace of meridians (IRRTM), liquid crystal granules, etc appearing along the meridian are associated with the specific transmission of biological information. The so-called "meridian" is referred to the specific connection regularities between one part and the other part of the body surface or between the body surface and the remote internal organs. Currently, the meridian research of our country is at the leading level in the world, but the breakthrough of study on meridian has a long way to go.关键词：中医经络;循经感传;经脉-脏腑相关;经脉线理化特性;文献研究1051|0|12更新时间：2023-08-11
- 摘要：目的 :对《针刺研究》的期刊学术水平作出客观评价。方法 :用引文分析法对《针刺研究》杂志 2 3期中学术性文章所引用的文献进行统计分析。结果 :本刊引文自引率、引文语种中的外文比例、平均引文量均保持在较高水平 ,分别为 1 1 .74%、3 3 .5%、8.2 0条 /篇 ;被引文献峰值是在其发表后的第 3年 ,半衰期为 6.6年 ,引文类型以期刊为主 ,这些均与资料所统计的其他医学期刊基本一致。根据布拉德福的离散定律 ,本文还统计出了针灸基础研究领域参考价值较大的 6种核心期刊 ,其中《针刺研究》列居榜首。结论 :该刊质量较好 ,流通较快 ,利用率也较高 ,在该领域的刊物中具有一定的影响力Objective:To set an objective value on the academic level of the journal Acupuncture Research.Methods:Total quoted references in the 23 current issues of Acupuncture Research were counted and analyzed by the method of bibliometric quotation analysis.Results:The ratio of self-quoted references,the proportion of foreign languages of the quoted theses and the average quotation amount of each thesis are all kept at a high level.They are 11.74%,33.5%,8.20 items per thesis respectively.The peak value of the quotation of the theses is at the third year after being published;the half-life is 6.6 years; and the main media of the quotation are journals.These are similar to the other medical journals counted up in the published papers.According to Bradford's dispersion law,this article also arranged six key journals in the field of acupuncture experiment. Among them the journal Acupuncture Research is listed as the first.Conclusion:Acupuncture Research has a better academic quality, a faster circulation and a higher utilization ratio.It has a certain influence in the journals of acupuncturology field.关键词：《针刺研究》;引文分析743|0|0更新时间：2023-08-11
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