最新刊期
2003年第1期
- 摘要：目的 :观察延髓孤束核P物质免疫阳性反应产物的分布以及针刺“足三里”穴对其的影响。方法 :将SD大鼠随机分为三组 :电针“足三里”穴组 (n =10 )、电针“足三里”穴旁开 0 5cm组(n =10 )、空白对照组 (n =10 )。应用免疫组织化学ABC法显示延脑组织SP免疫活性切片。结果 :孤束核内P物质的免疫阳性反应产物以膨体型纤维为主 ,呈点状和串珠状 ,其敞开部有少量阳性胞体。主要分布于孤束核的内侧亚核、连合亚核 ,其余亚核内较为稀疏 ;电针“足三里”穴内侧亚核、连合亚核阳性纤维的光密度明显升高 ,其余亚核无明显变化 ;电针“足三里”穴外侧旁开 0 .5cm组与空白组相似 ,各亚核均无明显变化。结论 :针刺“足三里”可能通过抑制NTS内侧亚核和连合亚核SP的释放 ,对胃的功能活动产生兴奋作用Objective: To observe the distribution of substance P like immunoreactivity(SP LI) in the nucleus of solitary tract(NTS)and the effect of electroacupuncture(EA) at "Zusanli" (ST 36) on SP. Methods: Thirty SD rats were randomly and evenly divided into control group, "Zusnali" group and Non acupoint (about 0.5 cm beside "Zusali") group. EA (sparse dense waves, frequency of 16～ 20 Hz and strength that is able to induce slight muscular tremor) was applied to bilateral "Zusanli" (ST 36) for 20 min.Avidin biotin peroxidase complex (ABC) method of immunohistochemistry was employed to display SP LI of the bulb tissue sections. Results: Under light microscope,SP immuno reaction products mainly were cirsoid fibers,presenting dot like or strings of bead like, distributing mainly in the medial and commissural subnucleus of NTS (mNTS, cNTS) and the spinal nucleus of trigiminal nerve; while no immunoreactive neurons were seen in NTS. After EA stimulation of "Zusanli " (ST 36) , the light density of SP LI in the mNTS and cNTS increased significantly in comparison with control group and non acupoint group (P<0.05～0.01). No significant changes were seen in other nuclei as dorsal motor nucleus of the vagus, area postrema, etc. There was also no obvious difference between non acupoint group and control group in SP LI changes in every subnucleus of NTS. It showed that EA of "Zusanli"(ST 36) could inhibit release of SP. Conclusion: NTS is an important primary centre responsible for the regulative action of EA of "Zusanli" (ST 36) on the physiological activities of the stomach.关键词：“足三里”;孤束核;P物质1037|0|0更新时间：2023-08-11
- 摘要：目的 :观察电针对神经细胞死亡、DNA损伤及凋亡相关基因蛋白 (Bcl 2、Bax、P53 )表达的影响。方法 :采用短暂脑缺血再灌注 (MCAo)模型 ,应用HE、TUNEL、免疫组织化学等实验技术进行观察。结果 :大脑中动脉阻塞 2hr后 ,随着再灌时间的增加梗塞灶面积进行性发展 ,在不同再灌时间点 ,电针均能减小梗塞面积 ,而且电针可明显减少短暂脑缺血再灌注后细胞坏死和DNA损伤 ,并上调Bcl 2 /Bax比值 ,和减少P53的表达。结论 :电针具有神经保护作用 ,其部分作用可能通过调节凋亡相关基因蛋白的表达而实现Objective: To observe the protective effect of electroacupuncture (EA) against rat's cerebral ischemia induced by middle cerebral artery occlusion (MCAo). Methods: 30 SD rats were randomized into ischemia (2 hr) and reperfusion (IR) control group, and IR+EA group. Each group was further divided into three subgroups respectively according to the different time course of reperfusion: IR 24 hr group, IR 48 hr group, IR 72 hr group, and EA+IR 24 hr group, EA+IR 48 hr group and EA+IR 72 hr group, with 5 rats in each group. EA was applied to "Baihui" (GV 20) and "Renzhong" (GV 26) for 1 hr beginning from 15 min on after ischemia. After 24 hours' reperfusion, EA was performed on the same two points for 45 min everyday. Neuronal death and apoptosis associated proteins (Bcl 2, Bax,P53) expressions of the cerebral tissue sections were observed using HE staining, TUNEL (TdT mediated dUTP Nick End Labeling) and immunohistochemistry. Results: After IR, the cerebral infarction area increased progressively along with the increase of duration of the reperfusion, while that of 3 EA groups was smaller. There were significant differences between IR 24 hr group and EA+IR 24 hr group, between IR 48 hr group and EA+IR 48 hr group, and between IR 72 hr group and EA+IR 72 hr group ( P <0.05～0.01).The surviving rates of neurons in the striate body of the brain of EA+IR 24 hr group and in the cerebral cortex of EA+IR 48 hr group and EA+IR 72 hr group were significantly higher than those of the corresponding IR control groups respectively ( P <0.05).The TUNEL positive cell ratio of the cerebral cortex in each one of 3 EA groups was significantly lower than that of each corresponding IR control group ( P <0.05～0.001). The ratio values of Bcl 2 positive cells/Bax positive cells of 3 EA groups were all significantly higher than those of the 3 corresponding IR control groups ( P <0.05～0.001). P53 immuno reaction positive neurons of EA+IR 24 hr group and EA+IR 48 hr group were markedly fewer than those of IR 24 hr group and IR 48 hr group respectively ( P <0.05). It showed that EA could evidently reduce the neuronal death and apoptosis, up regulated Bcl 2/Bax and down regulated the expression of P53 in cerebral IR rats. Conclusion: The protective effect of EA on cerebral ischemic neurons may be related with the resultant regulation of the expression of apoptosis associated proteins (Bcl 2, Bax, P53).关键词：脑缺血;电针;DNA损伤;细胞凋亡1059|0|5更新时间：2023-08-11
- 摘要：目的 :研究头皮针对脑缺血大鼠模型碱性成纤维细胞生长因子的影响 ,并与电针组比较。方法 :线栓法制备大鼠脑缺血模型 ,甲醛固定脑组织切片 ,运用免疫组化方法检测各组碱性成纤维细胞生长因子的变化情况。结果 :术后 6hr与术后 1 5天组内神经功能评分比较 ,模型组无明显差异 (P >0 .0 5) ,电针组与针刺组有显著性差异 (P <0 .0 1 )。组间 1 5天时比较 ,电针组与针刺组无明显差异 (P >0 .0 5) ,两组与模型组比较均有显著性差异 (P <0 .0 1 )。在第 1 5天时碱性成纤维细胞生长因子免疫组化染色比较 ,电针组与模型组有明显差异 (P <0 .0 5) ,针刺组与模型组有显著性差异 (P <0 .0 1 ) ,针刺组与电针组有明显差异 (P <0 .0 5)。结论 :头皮针可促进碱性成纤维细胞生长因子产生并延长碱性成纤维细胞生长因子产生时限 ,这可能是头皮针减轻脑缺血损伤并促使肢体功能恢复的机理之一Objective: To study the effect of scalp acupuncture on cerebral basic fibroblast growth factor (bFGF) expression in cerebral ischemia model rats. Methods: A total of 50 Wistar rats were randomized into control group, sham operation group, model group, electroacupuncture (EA) group and hand acupuncture group, with 10 cases being in each group. Cerebral ischemia model was established by obstructing blood flow of the right middle cerebral artery with a nylon thread embolus. Two acupuncture needles were inserted into "Baihui" (GV 20) and "Qubin" (GB 7) separately to advance oppositely along the scalp, with the scalp points stimulated with EA or hand manipulation. The treatment was given once daily, continuously for 15 days. The brain tissue sample was fixed with formaldehyde, sliced up, and then stained with immunohistochemical technique for observing changes of bFGF immuno reaction (IR) positive neurons of the cerebral tissue. Results: Auto comparison between 6 hr and 15 d after operation in neurological defect score showed no significant difference in model group ( P >0.05), but in EA group and hand acupuncture group this neurological defect score decreased apparently after treatment ( P <0.01). There were notable differences between EA group and model group, and between hand acupuncture and model group in the neurological score ( P <0.01); while no distinct difference was found between EA group and hand acupuncture group ( P >0.05), suggesting a favorable effect of acupuncture in improving cerebral ischemia induced neurological functional disturbance. Results of immunohistochemical staining displayed that bFGF expression in model group appeared in 3 animals (no bFGF expression in control and sham operation groups); the density of bFGF IR in both EA group and hand acupuncture group increased strikingly compared with model group ( P <0.05～0.01); and the effect of hand acupuncture group was superior to that of EA group ( P < 0.05 ). It suggests that acupuncture induced up regulation of bFGF expression maybe contribute to its therapeutic effect in bettering cerebral ischemia and promoting the recovery of the limbs after stroke. Conclusion: Scalp acupuncture can up regulate the expression of bFGF in the brain tissues of the cerebral ischemia rats.关键词：头皮针;脑缺血;碱性成纤维细胞生长因子858|0|4更新时间：2023-08-11
- 摘要：目的 :研究针刺对链脲佐菌素致糖尿病大鼠学习记忆障碍的防治作用。方法 :在箱底有金属栅栏的跳台箱内训练大鼠。以主动回避反应作为学习记忆能力指标。训练时 ,短声信号先出现 5sec,然后结合脚掌电击 1 0sec,大鼠能在声信号期内跳上绝缘平台主动回避电击被认为条件反射建立。海马薄片LTP记录 :在海马CA1 区记录由刺激Scheffer侧支诱发的突触后群峰电位(PS) ,并观察高频刺激 ( 1 0 0Hz,1sec)引起的长时程增强效应 (LongTermPotentiation ,LTP) ,作为突触可塑性的指标。结果 :糖尿病大鼠在跳台训练中正确建立条件反射的百分率明显低于正常组 (P <0 .0 5) ,而针刺组显著优于糖尿病组 (P <0 .0 5)。糖尿病组LTP效应较正常组明显减弱 (P<0 .0 0 1 ) ,针刺组较糖尿病组明显改善 (P <0 .0 5)。结论 :糖尿病大鼠在行为学和海马LTP记录中均表现出学习记忆能力的损害 ,针刺治疗在一定程度上可增强神经可塑性 ,改善学习记忆障碍Objective:The effects of acupuncture treatment on learning and memory decline were studied in diabetes mellitus (DM) rats. Methods: Streptozotocin induced diabetic rats were trained to make an active avoidance reaction (index of learning memory) in a compartment equipped with a grid floor through which an electric shock (40 V) was delivered. The training started with sound signal (5 sec), then combined with foot shock for 10 sec. If the rats jumped onto the insulated platform located in the middle of the grid floor to avoid foot shock within 5 sec, the conditioned reflex was considered to have been established. In vitro experiments, the post synaptic population spikes (PS) were recorded from CA 1 area of the hippocampus in the prepared hippocampal slices by stimulating the Schaffer collateral pathway. Following a high frequency tetanic stimulation (100 Hz), the PS was augmented and lasted for 2 hours, that is called as long term potentiation (LTP) and used as an index of synaptic plasticity. In vivo study, 12 male Wistar DM rats were randomly and evenly divided into model group and acupuncture group, other 6 normal rats were attributed to normal control group. "Zusanli" (ST 36), "Pishu" (BL 20), "Shenshu"(BL 23) and "Sanyinjiao"(SP 6) were punctured and stimulated with uniform reinforcing reducing technique. Results: The correct rates for establishing the conditioned reflex in 40 times of training in normal control group, model group and acupuncture group were 44.8±16.4%, 17.0±17.9% and 45.5±17.3% respectively, showing that the correct rate of model group is significantly less than that of normal control group ( P <0.05), while that of acupuncture group is significantly higher than that of model group ( P <0.05). It suggests that the learning memory ability of DM rats declined remarkably and was improved after acupuncture treatment. Results of vitro study showed that the mean values of the amplitude and slope of PS in responding to high frequency stimulation in normal control, model and acupuncture groups increased by 180.8±8.4% and 214.5±6.6%, 124.9±6.2% and 141.3±11.5%, 146.4±4.5% and 177.2±8.6% respectively, which displayed that LTP was reduced and shortened considerably ( P <0.001) in DM rats, but markedly improved ( P <0.05) in acupuncture group. Conclusion: Both the learning memory ability and hippocampal LTP were severely impaired in DM rats. After acupuncture treatment, the hippocampal synaptic plasticity was enhanced apparently and the learning memory decline was also improved significantly.关键词：糖尿病;针刺;学习记忆障碍;LTP926|0|4更新时间：2023-08-11
- 摘要：目的 :观察Ⅱ型糖尿病 (NIDDM)大鼠模型下丘脑弓状核 (ARC)神经细胞自发放电和针刺对它的影响 ,探讨针刺治疗NIDDM的中枢机制。方法 :采用小剂量腹腔注射链脲霉素 (STZ)结合高热量饮食制造NIDDM大鼠模型 ,应用神经细胞微电极记录方法和脑立体定位技术观察NIDDM大鼠ARC神经细胞自发放电的变化 ,并观察针刺“足三里”、“内庭”、“胰俞”(治疗 4周 )对ARC神经细胞自发放电频率的影响。结果 :治疗前ARC神经细胞自发放电频率为 3 .9± 0 .8Hz(正常组为 1 1 .2± 2 .8Hz) ,治疗后为 1 3 .1± 3 .3Hz,治疗前后比较 ,P <0 .0 0 1。结论 :针刺对NIDDM大鼠ARC的良性作用可能是其取效的中枢作用机制之一Objective: To observe the effect of acupuncture on neuronal discharges of hypothalamic arcuate nuclei (ARC) in type Ⅱ diabetes rats for exploring the neural mechanism of acupuncture in treatment of diabetes. Methods: SD rats were randomly divided into control group (n=20), model group (n=22) and acupuncture group (n=21). Non insulin dependent diabetes mellitus (NIDDM, i.e. type Ⅱ diabetes) rat model was established by intraperitoneal injection of streptozotocin (STZ, 25 mg/kg/day) for two days, followed by immediately feeding high fat diet for 12 weeks. "Zusanli"(ST 36) and "Yishu"(EX 17) were punctured with filiform needles and stimulated with uniform reinforcing reducing manipulation for 1 min; and "Neiting"(ST 44) was stimulated with twirling reducing method for 30 sec first, then these acupoints were stimulated with electroacupuncture therapeutic apparatus (10 Hz, 1.5 V, continuous waves) for 15 min. Acupuncture treatment was conducted once daily, continuously for 4 weeks. Blood sugar was measured before and after treatment. Neuronal discharges of hypothalamic ARC were recorded with glass microelectrodes (filled with 3 M KCl+ 2% pontamine sky blue) in other 18 rats, with 6 animals in each group. Results: Before and after acupuncture treatment, blood sugar values of control, model and acupuncture groups were 7.25±1.07 mmol/L, 7.67± 1.83 mmol/L; 16.41±5.30 mmol/L, 14.28±7.04 mmol/L; 16.39± 4.78 mmol/L, 11.18± 5.12 mmol/L, indicating that acupuncture could lower the increased PBG in NIDDM rats. In model group, the frequency of ARC neuronal discharges was 3.9±0.8 Hz (that of normal rats being 11.2± 2.8 Hz), while in acupuncture group, it was 13.1±3.3 Hz, being significantly higher than that of model group (P<0.001). Conclusion: Results of the present paper suggest that acupuncture can raise neuronal firing rate of ARC which may be related to the regulative effect of acupuncture on type Ⅱ diabetes.关键词：针刺;NIDDM;ARC;神经元自发放电927|0|0更新时间：2023-08-11
- 摘要：目的 :了解电针对面神经损伤后再生微环境中神经营养因子 (NGF)的影响。方法 :采用同龄成年健康的新西兰兔 50只 ,随机分为针刺组和对照组 ,均在 3 %的戊巴比妥钠静脉麻醉下 ,分离暴露面神经上颊支 ,在手术放大镜下切断神经 ,用硅胶管将两断端嵌入并缝合固定 ,形成再生室。针刺组于术后当天完全醒后开始接受电针治疗 ,每天 1次 ,对照组不作任何处理。术后3天、5天、7天、1 0天、1 4天分别处死 1 0只动物 ,针刺组与对照组各 5只。抽取再生室内液体 ,两组对照。结果 :在伤后 3～ 7天 ,针刺组和对照比较再生室内NGF浓度差别无显著性 (P >0 .0 5) ,接受电针治疗 1 0天和 1 4天 ,针刺组再生室内NGF浓度比对照组高 (P <0 .0 1 ) ,伤后第 7天 ,针刺组和对照组再生室内NGF浓度均达到峰值 ,但对照组在伤后 2周开始降低 ,针刺组仍保持在较高的水平。结论 :电针对面神经损伤后再生微环境中神经生长因子 (NGF)的浓度有提高其水平和维持平稳水平的作用 ,这可能是电针刺激促进周围神经再生机理的一方面Objective: To observe the effect of electroacupuncture (EA) on NGF level in the injured facial nerve regeneration chamber in the rabbits. Methods: Facial nerve injury was induced by transectioning the upper buccal branch of the facial nerve about 5 mm in length that was then fixed in a 12 mm long silica gel tube (chamber). 50 facial nerve injury rabbits were randomized into control and EA groups, with 25 cases in each group. EA (18～20 Hz, 1.5 V, 30 min/time) was applied to "Yifeng" (TE 17), "Dicang" (ST 4) to "Jiache" (ST 6) and "Hegu" (LI 4), once daily, continuously for 3～14 days. EA and control groups were respectively divided into 3 d, 5 d, 7 d, 10 d, 14 d subgroups. The fluids samples accumulated in the chamber were collected at different postoperative intervals: 3 d, 5 d, 7 d, 10 d, 14 d for detecting NGF content using double antibody Sandwiched ELISA. Results: In 7 d control and EA subgroups, NGF levels peaked, and decreased in 10 d and 14 d control subgroups, suggesting that EA can maintain the higher secretion level. NGF contents of the 5 EA subgroups particularly 10 d and 14 d subgroups ( P <0.01) were all higher or significantly higher than those of the corresponding 5 control subgroups, indicating that EA treatment can potentiate the secretion of NGF of the injured facial nerve and the effect of EA peaked on the 10 th day. In 7 d control and EA subgroups, NGF levels peaked, and decreased in 10 d and 14 d control subgroups, suggesting that EA can maintain the higher secretion level. Conclusion: Electroacupuncture therapy can promote the secretion of NGF of the injured nerve which may contribute to the effect of acupuncture in promoting the regeneration of the injured nerve.关键词：电针;神经营养因子;神经再生948|0|0更新时间：2023-08-11
- 摘要：目的 :探讨穴注黄芪、当归注射液对慢性萎缩性胃炎 (CAG)胃粘膜血流的影响。方法 :采用N 甲基 N 硝基亚硝基胍 (MNNG)诱发大鼠CAG。随机将 70只大鼠分为正常组、模型 1组( 40 μg/mLMNNG造模 )、模型 2组 ( 60 μg/mLMNNG造模 )、穴注 1组 ( 40 μg/mLMNNG造模+穴注 )与穴注 2组 ( 60 μg/mLMNNG造模 +穴注 )。穴注组均于造模第 1 0周开始以黄芪、当归注射液等份混合注入“足三里”穴。造模第 3 1周时观察各组大鼠胃粘膜病理及胃粘膜血流的改变。结果 :随造模浓度的增加 ,大鼠胃粘膜血流量下降 ,胃粘膜损伤加重 ,二者呈负相关 ;同时胃粘膜肠化及异增程度加重 (P均 <0 0 5)。穴注组可明显增加胃粘膜血流量 ,减轻胃粘膜损伤 (P <0 0 5)。结论 :局部血流量减少是胃粘膜损伤与萎缩发生、进展至胃癌前病变的重要原因 ;穴注法可能通过改善胃粘膜血供、促进组织修复而防治CAG。关键词：慢性萎缩性胃炎;胃粘膜血流;穴位注射896|0|4更新时间：2023-08-11
- 摘要：目的 :观察不同剂量曲马多对关节炎大鼠热痛敏的镇痛作用及曲马多对针刺镇痛的影响。方法 :在完全弗氏佐剂关节炎大鼠模型上采用辐射热缩腿反射的方法 ,以痛敏分数作为观察指标 ,分别腹腔注射 (i.p .) 5mg·kg- 1 ·d- 1 、1 0mg·kg- 1 ·d- 1 、2 0mg·kg- 1 ·d- 1 曲马多和 1 0mL·kg- 1 ·d- 1 生理盐水 1 0天 ,记录每次给药 2 4hr后痛敏分数变化。继而选择 1 0mg·kg- 1 ·d- 1 曲马多与电针合用 ,观察单独应用 1 0mg·kg- 1 ·d- 1 曲马多、单独应用电针、曲马多与电针合用及1 0mL·kg- 1 ·d- 1 生理盐水四组 (均为 1 0天 ) ,每次给药或 /和电针 2 4hr后痛敏分数变化。结果 :i.p .5mg·kg- 1 ·d- 1 曲马多连续应用 1 0天时 ,大鼠痛敏分数无明显变化 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多 9天时大鼠痛敏分数显著提高 ;i.p .2 0mg·kg- 1 ·d- 1 曲马多 6天时大鼠的痛敏分数显著提高 ;单独应用电针 8天时大鼠痛敏分数显著提高 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多与电针合用 4天时可明显提高大鼠痛敏分数。结论 :长期应用曲马多对慢性炎症痛大鼠具有镇痛作用 ,呈剂量相关性 ;曲马多与电针合用可加强电针的镇痛作用 ,并降低曲马多使用剂量。提示曲马多与电针合用是一种治疗慢性痛的有效方法Objective:To investigate the antinociceptive effects of chronically using different dose of tramadol and the effect of tramadol on acupuncture analgesia in arthritis rats. Methods: SD rat arthritis model was established by injection of complete Freund's adjuvant 0.05 mL into the left ankle joint. The paw withdrawal latency (PWA)was detected with a radiant heat algesimeter. The difference between PWA of the inflammatory side and the healthy side was used as the hyperalgesia score. By using paw withdrawal latency to radiant heat stimulation and hyperalgesia score as the endpoint of nociceptive test, 5 mg·kg -1 ·d -1 , 10 mg·kg -1 ·d -1 and 20 mg·kg -1 ·d -1 tramadol and 1 mL·kg -1 ·d -1 0.9% normal saline (NS) were respectively administrated intraperitoneally, once everyday and continuously for ten days. The changes of hyperalgesia score were observed 24 hr after drug administration everyday following i.p. 10 mg ·kg -1 ·d -1 tramadol, electroacupuncture (EA), EA combined with i.p.10 mg·kg -1 ·d -1 tramadol and i.p. 1 mL·kg -1 ·d -1 0.9% NS (4 groups), once everyday for ten days. Results: I.p. 5 mg·kg -1 ·d -1 tramadol for ten days did not show any effect ( P > 0.05) . I.p.10 mg·kg -1 ·d -1 tramadol for nine days, i.p. 20 mg·kg -1 ·d -1 for six days and using EA for eight days elevated hyperalgesia score significantly in comparison with NS group ( P <0.05～ 0.01) . Then i.p.10 mg·kg -1 ·d -1 tramadol combined with electroacupuncture for four days, hyperalgesia score was elevated significantly in comparison with NS group ( P <0.05～0.01) and with EA group ( P <0.05). Conclusion: The results show that tramadol has dose relative antinociceptive effect,and tramadol can enhance the analgesic effect of acupuncture under lower dose, suggesting that combination of acupuncture with tramadol is one of the effective treatment methods for chronic pain.关键词：关节炎大鼠;曲马多;电针;痛觉调制972|0|3更新时间：2023-08-11
- 摘要：目的 :观察褪黑素和电针对创伤大鼠脾脏淋巴细胞转化功能、ConA诱导的IL 2活性及脾淋巴细胞、血浆、下丘脑及垂体ACTH含量的影响。方法 :MTT法检测淋巴细胞转化功能、IL 2活性 ,放射免疫分析法检测各组织ACTH的含量。结果 :腹腔注射褪黑素能有效逆转手术创伤大鼠的淋巴细胞转化功能 ,低水平IL 2诱生活性得到恢复 ,同时创伤后升高的脾淋巴细胞、血浆、下丘脑ACTH水平下降 ,而垂体内降低的ACTH水平回升。电针刺激“足三里”、“阑尾”穴也能明显改善创伤应激诱导的免疫抑制 ,并降低脾淋巴细胞、血浆、下丘脑ACTH含量 ,增加垂体ACTH含量。褪黑素与电针合用可进一步调节被抑制的免疫功能 ,且与单独褪黑素或电针相比有显著差异。结论 :褪黑素和 /或电针能恢复创伤大鼠降低的淋巴细胞转化功能、IL 2诱生活性 ,并调节机体ACTH水平Objective: To observe the effect of melatonin (MT) and electroacupuncture (EA) on the spleen lymphocyte proliferation, concanavaline (Con) A induced IL 2 activity and ACTH content in the spleen lymphocyte supernate, plasma, hypothalamus and the pituitary of traumatic rats. Methods: SD rats with body weight of 200±20 g were randomly divided into normal control group, traumatic injury (TI) +MT solvent control group, TI+EA group, TI+MT group and TI+MT+EA group, with 6 cases in each group. Following anesthesia with pentobarbital sodium (0.4%, 2 mL), a longitudinal incision was made along the back and the abdominal midline to mimic clinical visceral exploring surgical operation for establishing traumatic injury model. EA (2 Hz, 1 mA) was applied to "Zusanli"(ST 36) and "Lanwei"(Extra 37) for 60 min before intraperitoneal (i.p.) injection of MT (5%, 5 mg/kg) that was given at 4～5 o'clock of the operation day. MTT colorimetric assay was used to detect lymphocyte proliferation rate and Con A induced IL 2 activity of the spleen lymphocyte supernate, and radioimmunoassay was used to detect ACTH contents in the spleen lymphocytes, plasma, hypothalamus and the pituitary. Results: Administration of MT and EA were able to revise TI generated depression of the lymphocyte proliferation rate and IL 2 activity ( P <0.05) compared with TI+MT solvent control group. In comparison with MT+TI and EA+TI groups, both the lymphocyte proliferation rate and IL 2 activity were raised further in TI+MT+EA group ( P <0.05). Intraperitoneal administration of MT and EA could considerably lower traumatic injury induced increase of ACTH levels in the spleen lymphocytes ( P <0.001), plasma ( P <0.05) and hypothalamus ( P <0.05), and significantly inhibit TI induced decrease of ACTH in the pituitary ( P <0.05, vs TI group respectively). When MT and EA used together, TI induced increase of ACTH levels in the spleen lymphocytes, plasma and the hypothalamus was lowered further ( P <0.05, vs TI+EA or MT group), while the decreased ACTH content in the pituitary was raised further ( P <0.05, vs TI+EA or MT group). Conclusion: MT and/or EA could recover TI induced changes of lymphocyte proliferation and Con A generated changes of IL 2 activity, modulate ACTH levels in the spleen lymphocytes plasma, hypothalamus and the pituitary in traumatic rats.关键词：创伤;褪黑素;电针;淋巴细胞转化功能;IL-2活性;ACTH1046|0|8更新时间：2023-08-11
- 摘要：目的 :了解分别电针心经、肺经循行路线上的 3个测试点以及不电针的对照组对心功能、小肠及脑电活动的影响。方法 :以左心室内压峰值 (LVP)、左心室内压变化上升最大速率(LVPdp/dtmax)、心力环面积 (ACFL)、心肌收缩成分最大缩短速度 (Vmax)、小肠电图及脑电图为指标 ,静脉滴注垂体后叶素造成心肌缺血动物模型进行观察。结果 :电针心经对心功能、小肠及脑电活动的调节作用比电针肺经显著 ,有相对特异性。结论 :心经作为一条经脉 ,与心功能、小肠及脑电活动有密切关系Objective:To probe into the effect of electroacupuncture (EA) of 3 points of the Heart Meridian and 3 points of the Lung Meridian on the cardiac function, small intestinal electrogram (SIEG) and electroencephologram (EEG) in acute myocardial ischemia (AMI) rabbits. Methods: A total of 30 rabbits anesthetized with urethane (1 g/kg) were randomized into control group, Lung Meridian group and Heart Meridian group with 10 cases in each group. AMI was induced by intravenous drip of posterior pituitary hormone. "Shenmen"(HT 7), "Lingdao"(HT 4) and the midpoint between them of the Heart Meridian, "Taiyuan"(LU 9), "Lieque"(LU 7) and the midpoint between these two acupoints of the Lung Meridian were punctured and stimulated electrically with ZYZ 1 EA Therapeutic Apparatus.Left ventricular pressure (LVP),dp/dtmax,the area of myocardial force loop (ACFL) and the maximal shortening velocity of the myocardium( Vmax ), SIEG and EEG were used as the indexes. Results: After AMI, LVP,dp/dtmax, ACFL,Vmax,the amplitudes of SIEG and EEG decreased significantly in comparison with their basic values ( P <0.05～0.001), and then recovered gradually 10～30 min later. No significant differences were found between Lung Meridian group and control group in the above mentioned indexes, but there were significant differences between Heart Meridian group and control group, and between Heart Meridian group and Lung Meridian group in all the indexes ( P <0.05～0.01).Conclusion: Results indicate that the regulative effects of EA of acupoints of the Heart Meridian are better than those of EA of acupoints of the Lung Meridian in improving AMI induced changes of the cardiac activity,SIEG and EEG. The whole Heart Meridian has a close relationship with the heart,the small intestine and the cerebral cortex.关键词：急性心肌缺血;电针;心经;肺经;心功能;小肠电图;脑电图1077|0|7更新时间：2023-08-11
- 摘要：目的 :研究电刺激束旁核对丘脑后核群内脏痛放电的影响。方法 :以电刺激内脏大神经在丘脑后核群诱发的单位放电作为内脏痛指标 ,应用细胞外玻璃微电极记录技术及条件检验刺激技术。结果 :束旁核对该内脏痛放电主要表现为抑制作用 ,抑制作用形式可分为即时抑制、延迟抑制及持续抑制三种。结论 :束旁核参与内脏痛的调制 ,主要为抑制内脏痛。关键词：内脏痛;束旁核;丘脑后核群;内脏大神经725|0|1更新时间：2023-08-11
- 摘要：目的 :利用三维重建软件在断面图像的基础上 ,在计算机上进行人体的重建和切割 ,从多层面显示穴位所处的各种解剖结构。方法 :将制作的风府穴断面标本拍摄成图像输入计算机 ,利用软件进行重建和切割。结果 :得到经风府穴的矢状、冠状、横断和斜切面。结论 :实体图像经软件重建和切割后 ,图像结构清晰 ,色彩鲜明 ,具有较高的分辨率Objective: On the basis of displaying sectional images, reconstruction and cutting of the human body were done using a three dimensional (3D) reconstruction software in computer for revealing the anatomic structure of Fengfu (GV 16) from different layers. Methods: A formalin fixed male corpse (2 years) was used in this study. ABS fluid mixed with scarlet dye was used for arterial perfusion to display large blood vessels. After locating Fengfu (GV 16) according to the national standards of acupoints and freezing the body (-30 ℃), a hole was made along GV 16 to the routinely used depth using a high speed bit, and then filled with red plasticine for displaying different cross sections of the acupoint. The frozen sample was then put into a wooden box which was filled with water to be frozen again, and was cut into less than 2 mm thick sections with a high speed steel plane machine, photographed and videotaped. The collected images were fed into a computer and reconstructed into facade and dorsal pictures with 3D reconstruction software, on the basis of which, sagittal, coronal and horizontal plane cutting of the body were carried out. Results: 200 pieces of reconstructed pictures of Fengfu (GV 16) were obtained. From the reconstructed sagittal plane and coronal plane images of GV 16 the blood vessels, muscular tissues, etc. could be seen distinctly. Additionally, other structures were also shown clearly. For example, the sagittal pictures of Fengfu (GV 16) distinctly displayed the appearance of the brain, cerebellum and the spinal cord. On the coronal plane, the images of GV 16 showed the fascia and muscle tissues. From the horizontal plane images, the appearance of the bottom of the cerebellar hemisphere, medulla oblongata, the head semispinal muscle, etc could be seen. Images of the oblique plane, the head splenius muscle (on one side of GV 16) and the head semispinal muscle (deep layer) could be seen. Conclusion: The reconstructed pictures of Fengfu (GV 16) from the entity images with the software show the clear structures, which has the bright color and satisfactory resolving power.关键词：断面解剖;三维重建;风府穴752|0|6更新时间：2023-08-11
- 摘要：目的 :为应用针刺预防和治疗老年性白内障提供理论依据。方法 :应用针刺疗法对 2 0例老年性白内障 (针刺治疗组 )进行术前治疗 ,然后应用等离子多通道光谱仪和激光散射浊度法测定术中抽取的房水中的钾 (K+)、钙 (Ca2 +)、磷 (P5 +)、镁 (Mg2 +)等离子元素及免疫球蛋白IgG、IgA、IgM、白蛋白、补体C3及C4含量 ;同步测定 2 0例成年健康组 (对照组 )及未经针刺治疗的老年性白内障患者 (白内障组 )的房水中的相同指标作为对照。结果 :白内障组房水中的K+、Ca2 +、Mg2 +等离子元素较对照组明显降低 ,而IgG、IgA、IgM、白蛋白、C3及C4含量均比对照组高 (P均<0 0 5) ,针刺治疗后可明显改善这种状况。结论 :针刺疗法可以调整老年性白内障患者房水中物理和生物化学环境 ,从而推测应用针刺疗法可以延缓老年性白内障的形成Objective:To observe the effect of acupuncture therapy on changes of ion element,immunoglubulin, albumin and complement levels of aqueous humor in order to provide a theoretical basis for the treatment and prevention of senile cataract with acupuncture. Methods: A total of 40 senile cataract outpatients were randomly divided into acupuncture group ( n =20)and cataract control group ( n =20). In the former group, Jingming (BL 1), Yuyao (EX HN 4),Qiuhou (EX HN 7), Sizhukong (TE 23), Chengqi (ST 1), Cuanzhu (BL 2), Ganshu (BL 18) and Shenshu (BL 23) were punctured with Gauge 28 filiform needles, with the needles retained for 30 min. Acupuncture treatment was conducted twice daily. Following 10 days' treatment, aqueous humor samples were collected. In cataract control group, the aqueous humor samples were collected from senile cataract patients during operation treatment.In addition, from other 20 cases of accidental death adult healthy patients,the aqueous humor was also sampled to be used as the normal control group. Contents of ion element K +,Ca 2+ ,Mg 2+ and P 5+ ,IgG,IgA,IgM,albumin,C 3 and C 4 of aqueous humor samples were assayed by using laser nephelometric method and an inductively coupled plasma(ICP) spectrometry. Results:① It was found that the levels of K +,Ca 2+ ,Mg 2+ and P 5+ in aqueous humor of senile cataract patients are significantly more than those of normal control people ( P <0.05);after acupuncture treatment, these elements increased obviously ( P <0.05) in spite of being still apparently lower than normal control group ( P < 0.05 ). ② In cataract control group,IgG, IgA, IgM,albumin,C 3 and C 4 contents of aqueous humor were strikingly higher than those of normal control group( P <0.05), while after acupuncture treatment,they all increased obviously( P <0.05)in spite of being still apparently lower than those of normal control group ( P <0.05).Conclusion: Acupuncture can regulate the physical and biochemical conditions of the aqueous humor of senile cataract patients.Thus,it is possible that acupuncture could delay the formation of senile cataract.关键词：老年性白内障;针刺疗法;房水;元素;免疫系统950|0|0更新时间：2023-08-11
- 摘要：从微观水平研究针灸效应对揭示其机理有重要的意义。本文从第二信使系统和其介导的基因表达对针灸效应影响的研究方面综述了针灸效应的终结反映于细胞内生物效应的变化(即对机体不同器官组织细胞内的物质代谢调节 ) ,从一个侧面提示 ,针灸对机体各系统的调节作用最终是在细胞通过胞内信息传导系统实现的In the present paper,the authors review recent progresses of researches on the mechanisms of acupuncture in regulating organism's functional activities via the second messenger system and gene expression.① Quite a few studies prove that the second messenger system including cAMP,cGMP,Ca 2+ ,myoinositol triphosphate (IP 3) and diglyceride (DG) is involved in the meridian phenomenon or the effect of acupuncture, for example, cAMP and cGMP in the brain participate in the analgesic effect of electroacupuncture, and the protective effect on ischemic cerebral cells via lowering calmodulin level. Ca 2+ level is higher in the tissues of acupoints and the meridian running trace. ② Acupuncture may evoke c fos expression of the paraventricular nucleus of the hypothalamus; up regulate the expression of heat shock protein of the cardiac myocytes in heart operation patients and cerebral ischemia rats; down regulate cerebral NOS and bax protein expression in cerebral ischemia rats; suppress the expression of IL 1β mRNA and IL 6 mRNA in the cerebral cortex, cerebellum and the pituitary of senile rats, etc.关键词：第二信使;基因表达;针灸效应1056|0|2更新时间：2023-08-11
- 摘要：本文旨在揭示针灸抗化疗骨髓抑制的机制 ,从理论上说明针灸疗效确切并为针灸治疗其他造血障碍性疾病提供依据。通过检索本领域相关文献进行总结与展望 ,认为针灸疗效确切 ,其机制涉及血细胞生成的动力学、白细胞生成的种类、造血干细胞的保护、体液性调节因子的活性等。造血微环境也参与调节 ,但还未深入研究In the present paper, the authors review advances in the investigation on mechanisms of acumoxi in resisting leukopenia due to chemotherapy induced myeloid inhibition from ① research methods; ② mechanisms of acumoxi in raising leukocytes from types of leukocytes; marrow cell classification; hemopoietic stem cell; hematopoietic humeral regulative factor; and extra marrow hematopoietic tissue; and ③ summary and prospects, and try to provide experimental evidence for acumoxi in treating other hemopoietic disorders. Through reviewing the studies in recent years on this subject, the present paper makes the conclusion and preview: Acumoxi therapy has the confirmed effects, its mechanism is of multi links, multi layers and multi levels. The dynamics of blood cell formation, the sort of leucopoiesis, protection of hemopoietic stem cells, and activity of humeral regulation factors are all involved. Besides, hemopoietic microenvironments are also involved in the regulation, but the further study has not been engaged.关键词：化疗;骨髓抑制;针灸;机制1160|0|4更新时间：2023-08-11
- 摘要：本文对近 5年针灸在抗早孕、引产催产方面的临床和实验研究的情况进行了总结。通过对 1 997年以来国内杂志相关文献进行综述 ,总结出在抗早孕方面 ,针灸主要应用于人流和药流的辅助治疗 ;在引产催产方面 ,针灸的应用与 5年前基本相同 ;在实验研究方面 ,主要是从电生理的角度验证和解释临床现象。针灸具有扩张宫颈、提高人流效果、镇静止痛、减少术中出血量等作用 ;针灸在药流中可加速排囊并提高完全流产率、减少出血、加快转经 ;针灸引产催产有效率较高 ,能促进宫颈成熟、缩短产程、减少产后出血量、减轻疼痛 ;实验研究发现针刺增强药流作用可能是通过调节子宫活动、改善妊娠相关组织血供而实现的In the present paper, the authors review advances of study on the anti pregnancy and labor inducing and labor hastening effects of acupuncture in recent 5 years in China. Clinical researches demonstrate that acupuncture therapy (otopoint pellet pressure method, body acupuncture) is mainly used to assist treatment of artificial abortion and drug abortion, and the clinical practice shows that acupuncture has functions of dilating cervix, relieving pain, enhancing the effect of drug abortion, reducing bleeding amount, shortening the birth process,calming the mind, etc. In addition, moxibustion is also applied in assisting drug abortion and has a synergistic action. Experimental researches display that the effect of acupuncture on potentiating drug abortion is realized by suppressing the non sequential contraction of the uterus, increasing the intra uterine pressure and raising the sensitivity of the gravida to drug. Acupuncture induced increase of plasma TXB 2 level, imbalance of thrombxane/6 Keto prostaglandin F 1α and contraction of the micro vessels might be responsible for its effects in reducing bleeding and potentiating uterine regular contraction. The effect of acupuncture in raising the sensitivity of pregnant women to drug is probably due to the increase of NOS activity of the chorion tissue, acceleration of the degeneration and necrosis of the villous trophocytes and shortening of the duration of embryonic sac ejection.关键词：针灸;抗早孕;引产;催产1095|0|8更新时间：2023-08-11
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