最新刊期
2003年第2期
- 摘要：目的 :观察针刺对大鼠大脑中动脉栓塞再灌注后的神经保护作用。方法 :将 3 0只大鼠随机分为正常组、模型组和针刺组。模型组和针刺组均用栓线插入大鼠大脑中动脉根部 ,2hr后抽出 ,使缺血组织再灌注 ,复制可逆性大脑中动脉栓塞 (rMCAo)模型 ,正常组和模型组不经任何治疗 ,针刺组分别在模型复制前 1hr、模型复制后 8hr和 1 6hr电针“水沟”、“太冲”、“曲池”、“足三里”、“丰隆”。观察神经行为症状 ,2 4hr后断颈处死大鼠 ,检测血液流变学指标 ,脑组织TTC染色后测量脑梗死及水肿体积 ,行病理组织学检查。结果 :神经行为学针刺组与模型组比较差异有显著性 (P <0 0 1 ) ;脑梗死和脑水肿体积百分率针刺组明显低于模型组 (P <0 0 1 ) ;血液流变学中全血比粘度针刺组与模型组比较差异有显著性 (P <0 0 5) ,但血浆比粘度、红细胞压积无明显变化 (P>0 0 5) ;病理组织学观察表明 ,针刺对大鼠脑组织损伤有明显的保护作用。结论 :针刺对大鼠脑缺血再灌注损伤具有明显的保护作用Objective: To study the protective effect of acupuncture on cerebral neurons in cerebral ischenmia-reperfusion injury rats. Methods: Thirty Wistar rats were randomized into control group (n=10), model group (n=10) and acupuncture group (n=10). Cerebral ischemia-reperfusion model was established by reversible occlusion of the middle cerebral artery. Rats of acupuncture group were treated with electroacupuncture of "Zhigou" (TE 6), "Taichong" (LR 3), "Quchi" (LI 11), "Zusanli" (ST 36) and "Fenglong" (ST 40) (parameters: 3 Hz, continuous pulses, duration of 15 min, and strength of inducing light tremor of the local muscles), 1 hours before cerebral ischemia, 8 hours and 16 hours after cerebral ischemia respectively. The alterations of neurological symptoms and signs were observed. 24 hours after cerebral ischemia-reperfusion, these rats were killed for collecting blood samples from the tail vein and for taking the brain tissue samples. The indexes of whole blood viscosity, plasma viscosity and hematocrit were detected. The brain tissue was cut into sections to be stained with tetrazolium chloride and HE method separately. Results: Scores of neurological symptoms and signs of model group decreased significantly 24 hours after cerebral ischemia-reperfusion in comparison with those of normal control group and acupuncture group (P<0.01), suggesting an improvement of neurological behavior after acupuncture treatment. In comparison with model group, the volume values of cerebral infarction and brain edema of acupuncture group decreased significantly (P<0.01), and the whole blood relative viscosity was also reduced obviously (P<0.01), but no significant differences were found among the 3 groups in plasma viscosity and hematocrit (P>0.05). Under light microscope, the pathohistological section showed that the neuronal injury of acupuncture group is relatively lighter compared with that of model group. Conclusion: Acupuncture has a protective effect on ischemia-reperfuse damage of cerebral tissue in rats.关键词：针刺;脑缺血再灌注损伤;神经保护1068|0|3更新时间：2023-08-11
- 摘要：目的 :观察“十二井穴”针法对局灶性脑缺血大鼠TGF β1的时效性影响。方法 :采用光化学诱导Wistar大鼠一侧大脑造成局灶性脑缺血模型 ,施以“十二井穴”针法在造模后 1、3、6、1 2、2 4hr不同时间段开始进行针刺治疗 ,治疗 6天后处死大鼠 ,沿缺血中心制成冠状面切片 ,运用免疫组化染色观察各治疗组半暗带与对照组神经细胞TGF β1表达量的变化 ,并进行图像分析。结果 :各时段模型组TGF β1表达的总面积及 3、6、1 2、2 4hr各组的积分光度均比假手术及正常组明显升高 (P <0 0 5) ,针刺组 3、6、1 2、2 4hrTGF β1表达的总面积及 6、1 2、2 4hrTGF β1表达的积分光度均较同时段模型组明显升高 (P <0 0 5或P <0 0 1 )。结论 :针刺对保护半暗带神经元具有重要意义Objective: To observe changes of transforming growth factor (TGF)-β1 expression in neurons of the brain after cerebral ischemia and acupuncture of the "twelve Jing points" in the rats. Methods: 96 Wistar rats were randomized into model group (n=30), acupuncture group (n=30), sham-operation group (n=30) and control group (n=6), the former 3 groups were further evenly divided into 1, 3, 6, 12 and 24 hr subgroups. The rat focal cerebral ischemia model was made by photochemical method to block blood flow of the middle cerebral artery. The "twelve Jing points"(LU-11, LI-1, PC-9, TE-1, HT-9, SI-1) were punctured with filiform needles, starting respectively at 1, 3, 6, 12, 24 hr after establishing ischemia models, once daily, 6 times altogether. 6 days later, the rats were put to death for taking the brain tissue sample (ischemic region) that was then cut into coronal slices. Immuno-histochemical staining was performed for displaying changes of TGF-β1 expression of the cerebral neurons in the penumbra region. Then image analysis was conducted. Results: In comparison with sham-operation and control groups, TGF-β1 expression area of model group increased significantly (P<0. 01) from 3 hr on after cerebral ischemia. Compared with model group, TGF-β1 expression area of acupuncture group increased further significantly from 3 hr on after cerebral ischemia (P< 0.05,0.01). The integral luminosity value of various groups presented a similar tendency in spite of appearing later (6 hr after cerebral ischemia) in the significant difference. Morphological observation indicated that TGF-β1 expression came from microgliocytes and phagocytes. Conclusion: The results prove that acupuncture can significantly upregulate TGF-β1 expression, which maybe contribute to the protection effect of acupuncture on ischemic cerebral neurons.关键词：局灶性脑缺血;十二井穴/针刺疗法;TGF-β11080|0|4更新时间：2023-08-11
- 摘要：目的 :比较针刺不同穴组对全脑缺血再灌注大鼠外周血WBC(白细胞 )、血清TNF α(肿瘤坏死因子 α)、IL 6 (白细胞介素 6 )和脑组织自由基的影响。方法 :四动脉结扎法造成大鼠全脑缺血再灌注模型 ,分别针刺大鼠阳明经“曲池”、“足三里”穴 (针刺二组 )与针刺“曲池”、“足三里”合用督脉“后顶”穴 (针刺一组 ) ,测定外周血WBC、血清TNF α、IL 6值及脑组织LPO(过氧化脂质 )、GSH(谷胱甘肽 )含量。结果 :大鼠全脑缺血再灌注后 ,外周血WBC、血清TNF α、IL 6值及脑组织LPO含量上升 ,GSH含量下降 ,针刺二组可降低大鼠全脑缺血再灌注后升高的IL 6水平 ,降低脑组织LPO水平 (P <0 0 5) ,针刺一组可降低全脑缺血再灌注后升高的WBC、TNF α和IL 6水平 ,降低脑组织LPO水平 (P <0 0 1 ) ,提高GSH含量。结论 :针刺“曲池”、“足三里”合用督脉“后顶”穴比单纯应用“曲池”、“足三里”穴对全脑缺血再灌注大鼠外周血WBC、血清TNF α、IL 6和脑组织自由基的影响更强 ,从而表明对全脑缺血再灌注大鼠脑组织具有更好的保护作用Objective: To observe the effects of electroacupuncture (EA) of different acupoint groups on blood WBC (white blood cells), TNF (tumor necrosis factor)-α, IL (interleukin)-6 and cerebral free radical levels in cerebral ischemia-reperfusion rats.Methods:A total of 43 Wistar rats were randomly divided into normal control group (n=8), Sham-operation group (n=8), ischemia-reperfusion (model) group (n=9), acupuncture (A)-I group (n=10) and A-II group (n=8). A four-arteries-ligation method was used to make cerebral ischemia-reperfusion model. In acupuncture-I group, "Quchi" (LI 11) and "Zusanli" (ST 36) were punctured and stimulated electrically (30 Hz, 3～6 V, 20 min) and "Houding" (GV 19) was punctured and stimulated by manipulating the acupuncture needle with uniform reinforcing-reducing method. In acupuncture-II group, only EA was applied to LI-11 and ST-36 with the same parameters. Blood samples were collected from the abdominal aorta to be processed into supernatant for measuring TNF-α, IL-6 and free radicals with radioimmunoassay. Cerebral tissues were taken after decapitation to be processed into supernate for detecting GSH and LPO contents with a spectrophotometer (colorimetric analysis). Peripheral blood WBC count was detected with routine method.Results:In comparison with normal control and sham-operation groups, blood WBC count, serum TNF-α and IL-6 and cerebral LPO (lipid peroxide) increased significantly (P< 0.01～0.001),and GSH (glutathione) decreased significantly (P<0.001) in model group. After acupuncture treatment, compared with model group, WBC of A-I group, TNF-α, IL-6 and LPO of A-I group, and IL-6 of A-II group all declined considerably (P<0.05～0.01), while GSH of A-I group increased significantly (P<0.05), showing that acupuncture can apparently lower WBC, TNF-α, IL-6 and LPO and raise GSH content. Comparison between two acupuncture groups showed that only LPO of A-I group was significantly lower than that of A-II group (P<0.05). Conclusion:EA of "Quchi" (LI 11) and "Zusanli" (ST 36) combined with acupuncture of "Houding" (GV 19) is superior to simple EA of LI-11 and ST-36 in regulating blood WBC, serum TNF-α and IL-6, and cerebral tissue free radical levels in cerebral ischemia-reperfusion rats.关键词：针刺疗法;全脑缺血再灌注;WBC;TNF-α;IL-6;自由基1040|0|8更新时间：2023-08-11
- 摘要：目的 :探讨“冰片液”穴位注射对SD大鼠血脑屏障通透性的影响 ,为临床提高某些中枢神经治疗药疗效提供实验依据。方法 :采用“哑门”穴位注射自制“冰片液” ,观察不同浓度“冰片液”穴位注射对伊文斯蓝 (Even’sBlue,EB)透过血脑屏障的作用 ,并与电针刺激组比较。结果 :“冰片液”穴位注射和电针刺激均可促进EB透过血脑屏障 (P <0 0 0 1 ,P <0 0 5) ,穴位注射“冰片液”高、低剂量组均优于电针组 (P <0 0 5) ,但“冰片液”高、低剂量组间无明显差异 (P >0 0 5)。结论 :“冰片液”穴位注射是促血脑屏障开放的一种有效方法 ,其作用优于电针刺激。关键词：血脑屏障;穴位水针;冰片1037|0|7更新时间：2023-08-11
- 摘要：目的 :分析循经分布的Aδ类初级传入纤维在跨脊髓节段信息传递中机械感受特性的改变和意义 ,从而探讨外周神经参与经络活动的机制。方法 :自近中端断离循足太阳膀胱经分布的胸 9至胸 1 2脊神经背侧皮支 ,在其中一支上施加逆向电刺激 ,以相邻脊髓节段的一支上Aδ类单位放电为指征 ,测定单位的适应特性、机械感受阈值、感受野形状和面积、感受野的敏感点数及其位置、传导速度等机械感受特性。在相邻脊髓节段皮神经上施加逆向电刺激后 ,观察上述各种机械感受特性的改变。结果 :在 6 7只大鼠上观察了 90个Aδ类初级传入纤维的机械感受特性和跨节段刺激对这些特性的影响。快适应和慢适应单位分别占 3 1 1 %和 6 8 9% ,适应时程为 2 0 82±2 51sec (n =90 )。机械感受阈值 0 2 1± 0 0 3mN。平均感受野面积 1 7 76± 3 40mm2 ,感受野内敏感点数为 1 79± 0 1 1个 ,77 3 % (n =88)的敏感点循足太阳膀胱经背部二直行脉之间分布。逆向电刺激相邻脊髓节段皮神经背侧皮支后 ( 0 45mA ,0 1ms,2 0Hz,持续 1 0sec) ,71 9% (n =6 4)的单位感受野增大 ,全部单位感受野均值增大为 71 98± 1 3 0 7mm2 ,显著大于对照 ,P <0 0 0 1。每个感受野内平均敏感点数显著增至 3 0 8± 0 2 5个 ,P <0 0 1。关键词：Aδ类初级传入纤维;机械阈值;感受野;循经感传1012|0|3更新时间：2023-08-11
- 摘要：目的 :本实验观察炎症痛和针刺镇痛时 ,大鼠中脑导水管周围灰质 (PAG)部位I型白细胞介素 1受体基因 (IL 1RImRNA)表达的变化。方法 :实验分正常组、炎症痛组、假电针组、电针组。正常对照组大鼠脚掌注射生理盐水 ,炎症痛各组大鼠脚掌注射 2 %角叉菜胶 ( 1 0 0 μL)造成炎症痛模型。在角叉菜胶注射 3hr后大鼠处于一种稳定的痛敏状态。电针组大鼠于造模前电针将致炎侧“足三里”和“昆仑”穴 3 0min ,假电针组大鼠给予同样处理但不通电。实验采用原位杂交技术 ,观察脚掌注射角叉菜胶 3hr后大鼠中脑导水管周围灰质 (PAG)部位IL 1RImRNA表达的变化及针刺对其的影响。结果 :致炎后 3hr大鼠痛阈显著降低 ,大鼠PAG部位的IL 1RImRNA表达显著增加 ,假电针组大鼠该部位的IL 1RImRNA表达与炎症痛组相比无显著变化 ,而电针组大鼠该部位的IL 1RImRNA阳性细胞数与假电针组相比显著减少。结论 :炎症痛可引起大鼠PAG部位的IL 1RImRNA表达增加 ,而电针镇痛则抑制炎症痛引起的大鼠PAG部位IL 1RImRNA表达Objective: The aim of the present study was to observe the changes of expression of IL-1R(receptor)-I mRNA in rat periaqueductal gray after peripheral inflammation and electroacupuncture (EA) analgesia. Methods: Twenty-four SD rats were evenly divided into normal group, inflammation group, sham EA group and EA group. Rats of normal group received an intraplantar injection of 100 μL normal saline, and those of inflammation group, sham EA group or EA group received an intraplantar injection of 100 μL carrageenan (2%, 100 μL). EA stimulation (4～60 Hz,1～2～3 mA, every 10 min for each current strength) was applied to unilateral "Zusanli" (ST 36) and "Kunlun" (BL 60) 3 hr after intraplantar injection of carrageenan. Sham EA was performed in the same way as EA except no EA was given. The expression of IL-1RI mRNA in rat periaqueductal gray following peripheral carrageenan inflammation was measured using in situ hybridization technique. The paw withdraw latency (PWL) was detected before and after different treatment. Results: Three hours after injection of carrageenan, PWL values of normal control, inflammation, sham EA and EA groups were 13.34±2.11 sec, 2.03±0.16 sec, 2.27±0.18 sec and 5.16±1.26 sec respectively; while compared with inflammation and sham EA groups, PWL of EA group increased significantly (P<0.05). Following carrageenan inflammation, the expression of IL-1RI mRNA in rat periaqueductal gray was significantly increased (positive reaction cells: 60.86±7.38, vs control group: 13.67±2.74, P< 0.001). Sham EA did not influence the expression of IL-1RI mRNA (61.45±6.49 positive cells), but EA could significantly inhibit the expression of IL-1RI mRNA (37.08±6.36 positive cells, P<0.05, vs inflammation and sham EA groups) in the same central region in carrageenan inflammatory rat. Conclusion: Carrageenan inflammation increases the expression of IL-1RI mRNA in rat periaqueductal gray, and EA analgesia could significantly inhibit the expression of IL-1RI mRNA in carrageenan inflammatory rats.关键词：炎症痛;电针镇痛;I型白细胞介素-1受体mRNA;PAG1035|0|5更新时间：2023-08-11
- 摘要：目的 :观察艾灸对荷瘤小鼠免疫功能的影响。方法 :将 40只荷瘤小鼠随机分为四组 ,对照组、艾灸组、5 氟脲嘧啶 ( 5FU)组、5FU +艾灸组。艾灸组、5FU +艾灸组分别在“中脘”、“神阙”、“关元”穴隔药饼灸 ,隔天 1次 ,治疗 8次。 5FU组、5FU +艾灸组在腹部注射 0 2 5mg/ 0 1mL5FU ,3天 1次 ,共治疗 4次。所有治疗完毕后 2 4hr处死动物。测定小鼠T淋巴细胞、自然杀伤细胞 (NK)及进行淋巴因子激活的杀伤细胞 (LAK)杀伤功能检测。结果 :艾灸组CD3、CD4、CD4/CD8比率、NK细胞、LAK杀伤活性比 5FU组显著提高 (P <0 0 5、P <0 0 0 5、P <0 1、P <0 1、P <0 0 1 ) ,差异明显 ,具有显著性意义。结论 :艾灸能提高小鼠的免疫力 ,而 5FU有抑制作用 ,使荷瘤小鼠免疫力降低Objective: To observe the influence of moxibustion on the immunological function of the tumor-bearing mice. Methods: 40 tumor-bearing mice were randomly and evenly divided into control group, moxibustion group, 5Fu (5-fluorouracil) group, 5Fu plus moxibustion group. Herbal cake-separated moxibustion was applied to"Guanyuan" (CV 4),"Shenque"(CV 8) and "Zhongwan" (CV 12)(herbal cake about 0.5 thick),3 cones each time, once every other day for eight treatments. 0.25 mg/0.1 mL of 5Fu was injected intraperitoneally for 5Fu group and 5Fu plus moxibustion group, once every 3 days, four times in succession. At the end of the treatment as well as 24 hr after completion of treatment, the mice were killed by extraction of the eyeball for collection of blood samples. The tumor tissue was taken for weighting the tumor body. The spleen was taken to be processed into cell suspension for examining the function of T lymphocytes with direct immunofluorescence marking method and flow cytometer. Natural killer cell (NK) activity and lymphokine-activated killer cell(LAK) activity (lethal rates) were detected with an enzyme-linked immunosorbent assay reader for recording OD values. Results: CD3% and CD4%, NK cell and LAK activity of 5FU group were lower than those of control group, suggesting decline of the immunity after injection of 5FU. Comparison between 5FU and moxibustion groups showed that CD3%, CD4% and CD4/CD8 of moxibustion group were significantly higher (P<0.05～0.005) or higher (CD4/CD8) than 5FU group. CD4% of moxibustion plus 5FU group was also significantly higher than that of 5FU group (P<0.005). NK cell activity of moxibustion group, NK cell and LAK cell activity of 5FU plus moxibustion group were all significantly higher than those of 5FU group (P<0.05～0.01), showing an enhancement of the activity of NK and LAK cells by moxibustion. Conclusion: Moxibusion can improve the immune function in tumor-bearing mice.关键词：艾灸;荷瘤小鼠;免疫951|0|1更新时间：2023-08-11
- 摘要：目的 :探讨电针“中膂俞”对L 多巴诱发膀胱活动亢进的尿动力学影响。方法 :以L 多巴诱发的膀胱活动亢进为动物模型 ,在动物清醒和自由活动的状态下 ,应用连续膀胱测压技术动态观察电针“中膂俞”对排尿过程中尿动力学参数的影响。结果 :电针“中膂俞”可减少排尿频率 ,降低膀胱基础压 ,升高膀胱最大充盈压和排尿压 ,增加排尿量。结论 :电针“中膂俞”对L 多巴诱发的膀胱活动亢进具有良好的抑制作用Objective: To observe the effects of electroacupuncture (EA) of "Zhonglushu" (BL 29) on urodynamics in L-dopa induced bladder hyperactivity. Methods: 72 SD rats were randomly and evenly divided into normal group, model group and EA group. Overactivity model of the bladder was established by intraperitoneal injection of 5% benserazid (2.5 mL/kg) and 1% L-dopa(2.5 mL/kg). Before each experiment, a median incision of the bladder was performed for intubation to measure various parameters of urodynamics. 3 days later, continuous cystometrogram was recorded under conscious state and without any restraint for observing the effects of EA on L-dopa-induced bladder overactivity. For animals of normal control group, normal saline (2.5 mL/kg) was injected intraperitoneally, and those of model and EA groups were injected with benserazid and L-dopa. Bilateral "Zhonglushu" (BL 29) were punctured and stimulated with an EA therapeutic apparatus (5 Hz, 4～6 V and 30 min). Results: Before treatment, the basic pressure (Pbas), maximal filling pressure (Pmax), micturition pressure (Pm), micturition circle (MC), time of micturition (Tm), number of micturition (Nm) and volume of micturition (Vm) had no apparent differences among the 3 groups (P>0.05). Half an hour and an hour after treatment, compared with normal control group, Nm and Pbas of model and EA groups increased significantly (P<0.05), while Pmax, Pm and Vm of the latter two groups decreased considerably (P<0.05). In comparison with model group, one hour after treatment, Nm and Pbas of EA group were markedly lower, whereas Pmax, Pm and Vm were strikingly higher (P< 0.05). It indicates that EA can lessen micturion frequency and bladder basal pressure, and increase micturition pressure and micturition volume in bladder hyperactivity rats. Conclusion: EA of "Zhonglushu" can inhibit the hyperactivity of the bladder effectively.关键词：电针;“中膂俞”;膀胱活动亢进875|0|1更新时间：2023-08-11
- 摘要：目的 :研究电针“关元”、“三阴交”对围绝经期模型大鼠神经内分泌的调整作用。方法 :复制大鼠去卵巢模型 ,测定电针“关元”、“三阴交”对模型大鼠血清雌激素 (E2 )、促卵泡激素 (FSH)、黄体生成素 (LH)和下丘脑 β EP含量的影响。结果 :模型组较正常组血清E2 含量降低 ( 2 2 77±0 1 3 5ng/L) ,FSH、LH含量升高 ( 7 0 6 2± 1 3 56mIU/mL ,3 473± 0 3 55mIU/mL) ,下丘脑 β EP含量显著降低 ( 1 6 751± 1 484pg/mg) ;电针组较模型组血清E2 含量显著提高 ( 3 44 9± 0 1 53ng/L) ,FSH、LH含量显著降低 ( 5 71 4± 0 3 50mIU/mL ,2 6 3 8± 0 1 98mIU/mL) ,下丘脑 β EP含量显著升高 ( 2 2 1 2 2± 0 82 8pg/mg组织 )。结论 :电针“关元”、“三阴交”治疗围绝经期综合征通过调节生殖内分泌和调整自主神经功能紊乱发挥作用Objective: To investigate the effects of electroacupuncture (EA) of "Guanyuan" (CV 4) and "Sanyinjiao"(SP 6) on neuroendocrine function in perimenopause model rats.Methods:Rat perimenopause model was made by removing the ovarium. 40 SD female rats were randomly divided into control group, EA group, model group and estradiol group, with 10 cases in each group. Serum estrin (E)2, follicle stimulating hormone (FSH), luteotropic hormone (LH) and β-EP of the hypothalamus were determined with radioimmunoassay before and after EA of SP-6 and CV-4 (2～100 Hz, 2.5 V, 20 min/time, once daily, 10 days/therapeutic course). In estradiol group, muscular injection of estradiol (0.05 μg/10 g) was given to the rats, once every other day. Results:In comparison with control group, serum E 2 and hypothalamic β-EP of model group decreased significantly (P<0.05), serum LH and FSH of model group increased markedly (P<0.05). Compared with model group, serum E 2 and hypothalamic β-EP of EA group and estradiol group increased apparently (P<0.05), while LH and FSH of these two groups lowered remarkably (P<0.05). Findings indicated that EA treatment could significantly raise serum E 2 and hypothalamic β-EP, and obviously lower serum LH and FSH levels. Conclusion:Electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao"(SP 6) can regulate neuroendocrine function in ovariectomized rats, suggesting EA may play a role in improving menopause syndrome.关键词：围绝经期;电针疗法;血清E2、FSH、LH;下丘脑β-EP1885|0|7更新时间：2023-08-11
- 摘要：目的 :观察不同频率电针穴位的镇痛作用规律。方法 :采用电刺激正常人下肢胫后神经获得痛相关成分P2 50 —N350 复合波作为反映疼痛的客观指标 ,观察不同频率电针穴位对痛相关成分P2 50 —N350 波幅、潜伏期变化的影响。结果 :2Hz组针中 1 0、2 0minP2 50 —N350 波幅明显降低 ,差异显著 ,分别为P <0 0 1、P <0 0 0 1 ;40Hz组针中 2 0minP2 50 —N350 波幅明显降低 ,差异显著 ,P <0 0 0 1 ;2Hz、40Hz组针后波幅仍维持较低水平 ,差异显著 ,分别为P <0 0 0 1、P <0 0 1。结论 :2Hz、40Hz电针对SEPS 皮层痛成分有明显的抑制效应 ;针后具有强而持久的后效应Objective: To observe the regularities of the analgesic effect of electroacupuncture (EA) at different frequencies. Methods: A total of 29 cases of healthy volunteer subjects were randomly divided into control group (n=10), 2 Hz-EA group (n=10) and 40 Hz-EA group (n=9). Cerebral somatosensory evoked potential (SEP) was recorded by using NeuropacR-II recorder following electrical stimulation (a tolerable strength) of the right posterior tibial nerve (medial side of the ankle). EA ( 2 Hz, 40 Hz, 2～4 V, for 20 min) was applied to right Zhibian (BL 54) and Ciliao (BL 32). The P 250-N 350 of complex waves of SEP was regarded as the objective index to reflect pain. Results: In 2 Hz-EA group, during EA, the amplitude values of P 250-N 350 declined significantly at 10 min and 20 min (P<0.01, P<0.001), while no striking changes of the latency were observed (P>0.05). Such is the case in 40 Hz-EA group, additionally, during EA stimulation, the patients responded with pain-relief to a certain degree. Twenty minutes after cease of EA, the amplitude values of P 250-N 350 of 2 Hz and 40 Hz groups still remained rather low level (P<0.01,0.001, vs 10 min of EA), suggesting a post-effect of EA in analgesia. Conclusion: 1) Both 2 Hz and 40 Hz EA have an obvious inhibitory effect on the pain component of SEP induced by tibial nerve stimulation; and 2) there is an apparent after effect of analgesia from 2 Hz and 40 Hz EA.关键词：电针;体感诱发电位;针刺镇痛1206|0|0更新时间：2023-08-11
- 摘要：目的 :为临床应用“关节炎Ⅲ号”穴位贴敷治疗类风湿性关节炎时选择最佳配穴处方提供依据。方法 :大鼠足跖皮内注射福氏完全佐剂诱导关节炎模型 ,观察自制“关节炎Ⅲ号”不同组合穴位涂敷对模型大鼠关节炎指数、左右后肢跖围的动态变化和关节的病理变化 ,以及血清、脾、关节浸液中的白细胞介素 1 β(IL 1 β)与前列腺素E2 (PGE2 )的浓度变化的影响。结果 :各治疗组大鼠的关节炎指数、跖围与模型组大鼠比较均有显著降低 ,其中系统取穴组见效最快 ,远道取穴组次之 ,局部取穴组见效最慢。造模后大鼠关节有明显的病理改变 ,经治疗各组炎症级别有所下降 ,以系统取穴组最明显。造模后大鼠各样本的IL 1 β浓度均上升 ,各治疗组IL 1 β浓度与模型组比较有一定差异 ,但以关节浸液中的IL 1 β浓度的降低为明显 ,并以远道取穴的效果较好 ;不同组合穴位涂敷均能降低模型大鼠关节浸液中升高的PGE2 浓度 ,以局部取穴为好。结论 :“关节炎Ⅲ号”穴位涂敷对大鼠佐剂性关节炎有效与对IL 1 β和PGE2 的调节有关 ;不同组合的穴位涂敷表现出不同的疗效 ,其中整体疗效以系统取穴最佳 ,远道取穴免疫调节作用较强 ,局部取穴抗炎作用较好Objective:To observe the therapeutic effects of external application of "Arthritis-Ⅲ" (a Chinese medicinal herbal ointment ) to different acupoint groups in the treatment of adjuvant arthritis in the rat. Methods:Arthritis rats model was established by subcutaneous injection of complete Freund's adjuvant (CFA, 0.1 mL) into the right paw. Fifty SD rats were randomly divided into remote + local acupoint group, remote acupoint group, local acupoint group, model group, and normal control group , with 10 cases in each one. Self-prepared "Arthritis-Ⅲ" ointment containing Leigongteng (Tripterygium Wilfordii) etc. was applied to "Dazhui" (GV 14), "Shenshu" (BL 23) and "Taixi "(KI 3) for 5 min every time, twice daily, continuously for 14 days. Arthritis index (AI) was tested using Wood's method, and the plantar circumference of the hind legs of the rats was measured from the 19th day on after injection, once every 3 days. The anatomical changes of rats' paw tissues were observed based on Mosakazu's method after staining with HE technique. IL-1β and Prostaglandin (PG) E 2 concentrations in the serum and spleen and joint fluid were assayed separately. Results: On the 33rd day after injection of CFA, AI values of the 3 acupoint groups, particularly that of remote +local acupoint group, were significantly lower than that of model group (P<0.05～0.01). From the 22nd day to 31st day after injection of CFA, the plantar circumference values (right limb) of the normal control group and the 3 treatment groups (in remote acupoint group, from 25th day on, and in local acupoint group, from 31st day on) were all markedly lowered than those of model group (P<0.05～0.01). The inflammation degree of the 3 treatment groups, especially that of remote+ local acupoint group, was significantly lighter than that of model group (P<0.05). IL-1β concentrations in serum, spleen supernate and the left joint infusion of model group were higher than those of normal control group, and majority of those of the 3 treatment groups were considerably lower than those of model group (P<0.05～0.01); only those of remote acupoint group were close to those of control group (P>0.05). PGE 2 concentration value of serum in model group was considerably lower than that of normal control group, and those of the 3 treatment group were higher or apparently higher than that of model group (P<0.05). On the contrary, PGE 2 concentration value in the joint infusion of model group was markedly higher than that of control group, and those of the 3 treatment groups were all significantly lower than that of model group (P<0.01), only PGE 2 levels (serum and joint infusion) of the local acupoint group were close to those of normal control group (P>0.05). Conclusion: External application of "Arthritis-Ⅲ" to acupoints is effective in relieving symptoms and signs of arthritis, reducing inflammation reaction, and regulating IL-1β and PGE 2 levels of the tissues. Among them, the effects of improvement of symptoms and signs and pathological changes in remote +local acupoint group are the best, the effect of immunoregulation in remote acupoint group is stronger, and the anti-inflammation action of local acupoint group is better.关键词：佐剂性关节炎;穴位涂敷;白细胞介素-1β;前列腺素E21048|0|0更新时间：2023-08-11
- 摘要：目的 :进一步探索针刺对钙调素活性的影响 ,研究钙调素在经络活动、针刺效应中的作用。方法 :在前期有关研究的基础上 ,采用磷酸二酯酶法 (PDE)测定针刺“下脘”穴后 ,“下脘”穴局部及其同经的“巨阙”穴处的钙调素 (CaM)活性的变化。结果 :针刺“下脘”穴后 ,其局部钙调素的活性明显高于同经“巨阙”穴的活性 (P <0 0 1 ) ,且两穴的活性明显高于针刺前任脉组织的活性 (P<0 0 1 )。结论 :针刺可能对钙调素有一定的激活作用 ;针刺对钙调素的激活作用可能沿着经络系统传递Objective : To study the influence of acupuncture on the activity of calmodulin(CaM) and to analyze the effects of CaM in the activities of meridians and the effect of acupuncture. Methods: Forty Wistar rats were divided into "Xiawan" group (n=10), "Juque" group (n=10) and Conception-Vessel group (n=20). Under conscious state, the rat was fixed on an experimental board, "Xiawan" (RN 10) was punctured and stimulated with uniform reinforcing-reducing method at a frequency of 80 times/min, continuously for 1 min, and the acupuncture needle was retained for 5 min. Under ether-anesthesia, the rat's abdominal tissues along the Conception Vessel from "Shuifen" (RN 9) to "Juque" (RN 14) were taken before acupuncture, and the tissues around "Xiawan" (RN 10) and "Juque" (RN 14) collected after acupuncture for determining the activity of CaM using phosphodiesterase(PDE) technique. Results: Following acupuncture of "Xiawan" (RN 10), the activity of CaM of "Xiawan" (RN 10) area is obviously higher than that of "Juque" (RN 14) (P<0.01); and the activity of CaM in "Xiawan" (RN 10) and "Juque" (RN 14) are all higher than that of tissues along Conception Vessel (P<0.01). Conclusion: Acupuncture may activate the calmodulin(CaM), which is probably involved in the signal transmission of acupuncture along the meridians.关键词：针刺;钙调素活性;“下脘”;“巨阙”971|0|1更新时间：2023-08-11
- 摘要：目的 :探讨风池穴的解剖结构和针刺深度。方法 :随机抽样取 57具较新鲜的成年人尸体 ,其中男尸 2 4具 ,女尸 3 3具 ,采用解剖断面法和解剖层次法进行研究。结果 :向下直刺的解剖结构依次是 :皮肤、浅筋膜、斜方肌与胸锁乳突肌之间、头夹肌、头半棘肌、头后大直肌。针刺的平均危险深度是 49.71mm。结论 :为了安全 ,建议风池穴针刺的深度控制在 3 4.80mm之内Objective: To probe into the anatomic structure and safe needling depth of Fengchi (GB 20) acupoint. Methods: Fifty-seven fresher adult corpses were randomly selected. Among them, 24 were male and 33 female. Anatomic cross-sectional method and anatomic lamelled method were used to study the anatomic structure and needling depth. Put the studied corpse into the low-temperature refrigerator (below -30 ℃) for 4 days, then the tissues around Fengchi (GB 20) were sectioned horizontally. When the ice melted, the distance from the skin to the surface of the upper end of the spinal cord was detected with an electronic digital ruler. In 6 corpses for lamina analysis, the anatomical structure of GB-20 was observed. Results: The anatomic structures of insertion of an acupuncture needle in order are: skin, superficial fascia, tissues between trapezius muscle and sternocleidomastoid muscle, musculus splenius capitis, musculus semicpinalis, musculus rectus capitis posterior major and membrane atlantooccipitalis posterior. Conclusion: The mean dangerous depth for perpendicular insertion of acupuncture needle is 49.71 mm, and for safe needlingty, it is proposed that the depth for perpendicular needling is within 34.80 mm.关键词：风池穴;解剖结构;针刺深度995|0|2更新时间：2023-08-11
- 摘要：目的 :观察顶颞前斜线齐刺法治疗中风偏瘫的临床疗效。方法 :将 6 2例中风偏瘫患者采用随机区组法分为治疗组 ( 3 2例 )和对照组 ( 3 0例 ) ,治疗组采用顶颞前斜线齐刺法加常规体穴治疗 ,对照组采用顶颞前斜线常规刺法加常规体穴治疗。各治疗 3 0次。结果 :治疗组总体疗效明显高于对照组 (P <0 .0 5) ,在提高患者肌力方面也优于对照组 (P <0 .0 5)。结论 :顶颞前斜线齐刺法加体穴治疗中风偏瘫有确切疗效 ,且优于顶颞前斜线常规刺法。具有一定的临床指导意义Objective: To observe the therapeutic effect of triple puncture of scalp-point in the treatment of apoplectic hemiplegia. Methods: A total of 62 cases of apoplexy patients were divided into treatment group (n=32) and control group (n=30) using randomized block design. In treatment group, patients were treated with triple puncture of Dingnie Qianxiexian (MS 6) on the affected side and acupuncture of body acupoints; while in control group, MS-6 and body acupoints were punctured with conventional method. The treatment was conducted once daily, continuously for 30 times. Results: After treatment, of the 32 and 30 cases in treatment and control groups, 9 and 2 recovered basically, 15 and 7 experienced marked improvement, 4 and 9 had improvement, 3 and 9 had slight improvement, 1 and 3 had no apparent changes respectively. The therapeutic effect of treatment group was significantly superior to that of control group (P<0.05). Before treatment, the myodynamia of the upper and lower limbs in two groups had no striking difference, while after treatment, patients with the myodynamia above IV class in two groups all increased, but the effect of treatment group was evidently superior to that of control group (P<0.01). Conclusion: The therapeutic effect of triple puncture of scalp-point plus acupuncture of body acupoints is better than that of conventional acupuncture of scalp-point and body acupoints.关键词：中风偏瘫;齐刺法;常规针刺969|0|1更新时间：2023-08-11
- 摘要：目的 :探讨针刺对自发性男性不育症患者施行卵细胞内单精子注射 (ICSI)治疗术临床疗效的影响。方法 :2 2例ICSI治疗失败的自发性男性不育症患者经过每周 2次、为期 2个月的针刺治疗后再次行ICSI治疗 ,观察治疗前后精液指标 ,ICSI治疗中的受精率。结果 :针刺治疗后快速活动精子的比率 ( 1 8 3± 9 6 % )高于治疗前 ( 1 1 0± 7 5% ) ,差异极为显著 (P <0 0 5)。治疗后正常形态的精子所占的比率 ( 2 1 1± 1 0 4% )亦显著高于治疗前 ( 1 6 2± 8 2 % ,P <0 0 5)。针刺治疗后行ICSI治疗的受精率 ( 6 6 2 % )明显高于治疗前 ( 40 2 % ,P <0 0 1 )。治疗前后精子的浓度和总的精子活动率没有显著性的差异。结论 :针刺能使自发性男性不育症患者的精子质量提高 ,从而达到提高ICSI治疗中受精率的目的Objective:To investigate the clinic effect of acupuncture on idiopathic male infertility in accepting intracytoplasmic sperm injection (ICSI).Methods:Twenty-two cases of idiopathic male infertility patients failed in ICSI were treated with acupuncture twice weekly for 8 weeks, and then followed by accepting ICSI treatment again. Acupoints used were Baihui (GV 20), Neiguan (PC 6), Chize (LU 5), Xuehai (SP 10), Neiting (ST 44), Shenshu (BL 23), Sanyinjiao (SP 6), Qihai (CV 6), Guanyuan (CV 4), etc. Within 3 months after acupuncture treatment, the patients accepted ICSI treatment again. The sperm concentration, motility, morphology and fertility rates were observed.Results: Sperm quick motility after acupuncture treatment (18.3±9.6%) was significantly improved than that before acupuncture (11.0±7.5%, P<0.05). The normal sperm ratio was increased after acupuncture(21.1±10.4% vs 16.2± 8.2%,P<0.05). The fertility rates after acupuncture (66.2%) were obviously higher than that before acupuncture (40.2%,P<0.01). There was no significant difference in sperm concentration and general sperm motility before and after acupuncture. But among their spouses, 4 cases had been affirmed to be gravid, with the pregnancy rate being 18.2%.Conclusion:Acupuncture can improve infertility patients' sperm quality and raise fertility rate during accepting ICSI treatment.关键词：针刺;卵细胞内单精子注射;受精率749|0|1更新时间：2023-08-11
- 摘要：针刺作为一种有效的辅助治疗手段已被广泛应用于临床。近年来对针刺的镇痛作用及对功能性疾病的调节作用研究颇多 ,除应用传统电生理学方法外 ,还采用了分子生物学、免疫组织化学、脑功能成像等先进的技术来探讨针刺对脑的经典神经递质、神经激素、神经元代谢等的影响 ,为针刺的中枢调节机制提供了理论基础。本文主要就针刺的中枢作用及方法学等方面加以讨论Acupuncture has been being used widely in clinical treatment of many types of diseases as an alternative or complementary therapy. In addition, researches on the mechanisms of acupuncture in regulating functional activities of the body have achieved abundant valuable results. In the present paper, the authors review some new progresses of acupuncture research from 1) effect of acupuncture on central neurotransmitters; 2) acupuncture stimulation and changes of gene expression; and 3) acupuncture stimulation and changes of central images. In the scientific researches, many traditional and advanced biomedical techniques as electrophysiological, molecular biological, immunohistochemical methods, cerebrofunctional imaging, etc. have been adopted at the same time. Results of these studies provided abundant experimental evidence for acupuncture induced regulation effects on the brain. This article focuses on the cerebral effect and methodology of acupuncture.关键词：针刺;中枢机制;综述961|0|1更新时间：2023-08-11
- 摘要：本文综述了近年来针刺治疗糖尿病周围神经病变的临床及实验研究进展 ,从临床治疗及动物实验两方面阐述了针灸治疗本病的确切疗效 ,并进一步探讨了其发病及治疗机理。本文还对今后的工作重点和努力方向提出了建议和展望In the present paper, the authors reviewed the recent advances in clinical and experimental researches on treatment of diabetic peripheral neuropathy (DPN) by acupuncture. Many clinical researches show that both acupuncture therapy and Western medicines can improve clinical symptoms of pain, numbness, abnormal sensation, etc. of DPN. Acupuncture treatment can also low diabetes mellitus (DM) patients' blood lipid and blood viscosity levels, improve blood circulation and the renovation of the injured peripheral nerve. Animal experiments demonstrate that in DM rats,acupuncture can strengthen the conductance speed of the motor nerve, lower blood glucose, whole blood viscosity and plasma viscosity, total cholesterol and triglyceride levels; suppress the decreased activity of Na +-K +/ATPase of the sciatic nerve; and revise the decreased PGI 2 and the increased TXA 2 levels. This article also put forward some suggestions about clinical research on DM in the future.关键词：针刺疗法;糖尿病周围神经病变1028|0|2更新时间：2023-08-11
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