最新刊期
2003年第3期
- 摘要：目的 :探讨穴位针刺预处理抗脑缺血再灌注损伤的作用。方法 :先进行“肾俞”、“百会”穴针刺预处理 ,再采用颈动脉引流法脑缺血再灌注造模 ,石蜡包埋切片 ,HE染色 ,观察脑片缺血性病理变化 ,进行顶皮质Ⅰ区Ⅴ层幸存神经元记数。结果 :在不同再灌时间段 ,E0 .5hr组幸存神经密度显著高于D组、E1 .5hr组和E3hr组 (P <0 0 5) ,E3hr组与D组无差异 (P >0 0 5)。结论 :穴位针刺预处理具有抗脑缺血再灌注损伤作用 ,该作用以穴位针刺预处理后 0 5hr效果最佳Objective:To observe the effect of electroacupuncture preconditioning (EAP) on neurons of the cerebral cortex in cerebral ischemia-reperfusion (IR) rats. Methods: 95 Wistar rats were randomly divided into normal control group (group A, n=5), sham-ischemia control group (group B, n=15), EAP control group (group C, n=15), cerebral IR control group (group D, n=15), EAP and cerebral IR group (group E, n=45). E group was further evenly divided into 0.5 hr, 1.5 hr and 3 hr subgroups (i.e. IR was conducted 0.5, 1.5 and 3 hours after EA). Temporary cerebral IR injury model was established by using common carotid artery blood drainage method. "Shenshu" (BL 23) and "Baihui" (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus (8～80 Hz, dense-sparse waves, 3 V, 4×10 min). After treatment, these rats of group B, C, D and 3 subgroups of group E survived for 1, 3 and 7 days (i.e. IR-1d, IR-3d and IR-7d) respectively, and were then killed for taking out the brain tissue which was to be cut into sections after perfusion fixation, with the tissue sections being stained with HE method. Then the surviving neurons were counted under microscope. Results: There were no significant differences among group A, B and C at IR-1d and between group B and C at IR-3d and IR-7d in the density of the cerebral surviving neurons, indicating that sham-operation and EAP had no apparent influence on cerebral neurons. Compared with group A, the density values of neurons of group D at IR-1d, IR-3d and IR-7d all reduced significantly (P<0.05). Comparison among E 0.5 hr, E 1.5 hr and E 3 hr subgroups of group D,displayed that the density values of E 0.5 hr subgroup at different IR time-courses and E 1.5 hr subgroup at IR-3d and IR-7d all evidently higher than those of group D(P<0.05), suggesting that EAP has an apparent anti-IR injury action. But there were no significant differences among E 1.5 hr and E 3 hr and D groups at the 3 time-courses (P>0.05).Conclusion: EAP can resist the injury of neurons of the cerebral cortex induced by temporary cerebral ischemia-reperfusion, which is better at 30 min after EAP than at 90 min and 180 min.关键词：电针预处理;脑缺血再灌注损伤;幸存神经元密度1100|0|4更新时间：2023-08-11
- 摘要：目的 :观察电针对脑缺血大鼠谷氨酸、门冬氨酸、甘氨酸及氨基丁酸含量的影响。方法 :利用大鼠大脑中动脉阻塞再灌流模型 ,采用高效液相 色谱法测定四种氨基酸含量。结果 :大鼠局脑缺血 3 0min ,再灌流 4hr后 ,Glu、ASP含量明显升高 (P <0 0 1 ,P <0 0 5) ,GABA和Gly含量无明显变化 ;而经电针治疗组Glu、ASP含量显著下降 (P <0 0 1 ) ,GABA含量升高 (P <0 0 5) ,Gly含量无明显变化。结论 :电针可抑制兴奋性氨基酸的过量堆积 ,EAA的减少与GABA的增加相关Objective: To study the effects of electroacupuncture (EA) on glutamate(Glu), aspartate(Asp), Glycine (Gly) and γ-Aminobuyric acid(GABA) in the brain tissue in rats with cerebral ischemia-reperfusion (CIR).Methods: Twenty-four Wistar rats were randomly and evenly divided into control group, CIR group and CIR+EA group. CIR model was established by occlusion of the middle cerebral artery for 30 min and removing the occlusion for 4 hours. "Suliao" (GV 25) and "Baihui" (GV 20) were punctured and stimulated with an EA therapeutic apparatus (dense-sparse waves, 5/ 45 Hz, 0.3 mA) for 30 min. High pressure liquid chromatography (HPLC)-fluorescence assay was used to detect contents of Glu, Asp, Gly and GABA in rat brain tissue before and after EA. Results: Four and half hours after CIR, Glu and Asp contents of the brain tissue increased significantly(P< 0.01, P<0.05),Gly and GABA levels had no obvious changes. After EA treatment, cerebral Glu and Asp levels reduced considerably (P<0.01), GABA level increased strikingly (P<0.05), and Gly content had no evident change. Conclusion: EA can reduce excessive accumulation of excitatory amino acids (Glu and Asp) in the brain that may be related to EA-induced increase of GABA. The regulative action of EA on excitatory and inhibitory amino acid contents in the brain may contribute to EA's neuroprotective effect on ischemic cerebral tissue.关键词：电针;脑缺血;氨基酸946|0|3更新时间：2023-08-11
- 摘要：目的 :探讨早期针刺治疗抗脑缺血再灌注炎性损伤的机制。方法 :采用大脑中动脉线栓法制备MCAO缺血再灌注模型 ,应用原位杂交的方法观察脑缺血再灌注及电针对大鼠大脑皮质、纹状体IL 1 βmRNA、TNF αmRNA表达的影响。结果 :脑缺血再灌注后 ,缺血侧皮层及纹状体IL 1 βmRNA、TNF αmRNA免疫阳性细胞表达增高 (模型组与正常组、假手术组比较P <0 0 1 ) ;电针可显著降低缺血侧皮层及纹状体IL 1 βmRNA、TNF αmRNA免疫阳性细胞表达 (电针组与模型组比较P <0 0 1 )。结论 :早期针刺治疗可能通过下调缺血脑区IL 1 βmRNA、TNF αmRNA免疫阳性细胞而防治脑缺血再灌注炎性损伤Objective: To explore into mechanisms of early treatment of cerebral ischemia-reperfusion (IR) by acupuncture in resisting inflammation injury in rats. Methods: Forty Wistar rats were randomly and evenly assigned to control, sham-operation, IR-6hr, IR-12hr, IR-24hr, IR-6hr-EA, IR-12hr-EA and IR-24hr-EA groups. Cerebral IR model was established by occlusion of the middle cerebral artery for 30 min and then removal of the occlusion for 6, 12 and 24 hr respectively. Electroacupuncture (EA, 4～16 Hz, 1～3 V, 30 min) was delivered via acupuncture needles inserted into "Shuigou"(GV 26) and "Baihui"(GV 20). The expression of IL-1β mRNA?TNF-α mRNA of neurons of the cerebral tissue was displayed using in situ hybridization technique after IR and assayed with a spectrometer system. Results: When compared with control group, the expression of IL-1β mRNA and TNF-α mRNA in the cerebral cortex and striate body on the ischemic side in IR-6hr, IR-12hr and IR-24hr groups increased significantly (P<0.01); while compared with IR-6hr, IR-12hr and IR-24hr groups separately, the expression of IL-1β mRNA and TNF-α mRNA of IR-6hr-EA, IR-12hr-EA and IR-24hr-EA groups was down-regulated considerably (P<0.01) despite being still higher than those of control group evidently. It suggested that EA could suppress the IR-induced over-expression of IL-1β mRNA and TNF-α mRNA of neurons in the brain tissues. Conclusion:The protective effect of acupuncture on cerebral neurons under acute cerebral infarction condition is possible through its downregulative action on IL-1β mRNA and TNF-α mRNA expression.关键词：电针;脑缺血再灌注;IL-1βmRNA;TNF-α mRNA956|0|6更新时间：2023-08-11
- 摘要：目的 :研究不同时间窗电针对实验性脑缺血疗效的影响。方法 :采用光化学法诱导局灶性脑缺血模型 ,于造模成功后 0 5hr、1hr、3hr、6hr、1 2hr五个不同的时间窗开始电针 ,比较治疗 6天后急性局灶性脑缺血模型大鼠脑组织活性钙调素 (CaM)含量的变化。结果 :大鼠脑缺血后 ,模型组CaM的含量明显升高 ,与空白对照组和假手术组相比有显著性差异 (P <0 0 5) ;电针治疗组的CaM含量与空白对照组相比无统计学差异 (P >0 0 5) ,但超早期不同的时间窗电针组间差异不显著 (P >0 0 5)。结论 :针刺疗法超早期介入在大鼠局灶性脑缺血中发挥肯定的脑保护作用 ,但从造模后 0 5hr开始分设的五个不同时段组间不显示开始治疗时间窗与针刺疗效的正相关系Objective:To observe the influence of electroacupuncure (EA) at different therapeutic time windows on calmodulin(CaM) level in focal cerebral ischemic injury rats. Methods: Seventy-three Wistar rats were randomized into normal control (n=10), sham-operation (n=8), model (n=9), EA-0.5 hr (n=10), EA-1 hr (n=10), EA-3 hr(n=8), EA-6 hr (n=9) and EA-12 hr (n=9) groups. The rat cerebral ischemia model was established by middle cerebral artery occlusion (MCAO) with photochemical method to induce focal cerebral ischemia injury. EA (20 Hz, 1 min/time) was delivered to "Shuigou"(GV 26) through acupuncture needles 0.5 hr, 1 hr, 3 hr, 6 hr and 12 hr respectively following MCAO, once daily, continuously for 6 days. The active CaM content of the ischemic cerebral tissues was measured by using phosphodiesterase method. Results: In model group, CaM content of the ischemic cerebral region elevated distinctly in comparison with normal control group and sham-operation group(P<0.05). Following administration of EA 0.5 hr, 1 hr, 3 hr, 6 hr and 12 hr after MCAO, CaM contents of these 5 EA groups were all lowered evidently compared with that of model group(P<0.05). There were no evident differences between each different therapeutic time window groups and normal control group in CaM content (P>0.05), suggesting that acupuncture treatment given at these 5 time intervals can effectively reduce cerebral ischemia induced elevation of active CaM level of the rat brain tissue. Conclusion: When acupuncture treatment was administered in the early period of acute cerebral ischemia, the elevated active CaM level in the brain tissue can be corrected effectively.There is no obvious correlation between different therapeutic time windows and the effect of acupuncture.关键词：脑缺血;电针;治疗时间窗;钙调素1009|0|2更新时间：2023-08-11
- 摘要：目的 :观察电针人体合谷穴和痛刺激正中神经时 ,其传入冲动在中枢神经系统内的相互作用。方法 :按照受试者、记录者、施针者三盲的方法 ,对 1 2例健康人进行 4系列试验。系列Ⅰ为非痛单独电刺激正中神经 ;系列Ⅱ为痛单独电刺激正中神经 ;系列Ⅲ为同时电刺激正中神经与合谷穴 ;系列Ⅳ为同时电刺激正中神经与合谷穴旁。刺激正中神经的电流强度在Ⅱ、Ⅲ、Ⅳ系列中相同。利用NeuroScan公司ESI 1 2 8脑电记录仪记录从 64个头皮电极点引出的体感诱发电位 (So matosensoryEvokedPotentials,SEP) ,并比较各主波的绝对峰值和峰峰值及作二维地形图分析。结果 :系列Ⅰ未能引出明显的SEP晚成分 ;系列Ⅱ、Ⅲ、ⅣSEP中、晚成分N80 、P1 70 、N2 80 的绝对峰值大小关系为 :系列Ⅱ >系列Ⅳ >系列Ⅲ ,而早成分在 3系列中变化不明显。二维地形图显示 ,SEP早成分发生源位于对侧半球 ,晚成分位于中央略偏对侧 ,向前额部扩伸 ,系列Ⅲ 87 40ms以后各时间窗口信号强度明显弱于系列Ⅱ和Ⅳ。结论 :电针合谷穴明显抑制痛刺激正中神经引起的痛觉诱发脑电位 ,穴位的镇痛效果好于非穴位 ,大脑皮质在针刺穴位镇痛过程中具有时间依赖的电活动 ,痛觉诱发电位可以实时反映电针穴位的镇痛效果Objective:This study was undertaken to observe changes of pain-related cerebral somatosensory evoked potentials (SEPs) following electroacupuncture (EA) stimulation of Hegu(LI 4) in volunteer subjects for elucidating the cerebral cortical mechanisms of EA analgesia. Methods: SEPs from 12 healthy volunteer subjects (7 males and 5 females) were recorded using 64 channels NeuroScan (ESI-128) following electrical stimulation of the left median nerve (MN), Hegu (LI 4) and non-acupoint (1 cm beside LI-4, i.e., the ulnar margin of the first metacarpal bone on the back palm and at the same level with LI-4) that included stimulus series of Ⅰ (4～6.8 mA,MN),Ⅱ (10～13 mA, MN), Ⅲ (10～13 mA, MN+LI-4) and Ⅳ (10～13 mA, MN+non-acupoint). Peak values of N 20, P 40, N 80, P 170 and N 280, and peak-peak (PP) values of N 20-P 40, N 80-P 170, P 170-N 280 of SEPs obtained from 64 scalp electrodes before and after stimulation were recorded and analyzed. Results: Late components of SEPs could not be gained during non-painful stimulation (series Ⅰ). During painful stimulation (series Ⅱ～Ⅳ), the peak values of medium and late components (N 80, P 170 and N 280) of SEPs were the biggest for series Ⅱ, moderate for series Ⅳ and the lowest for series Ⅲ. The early components (N 20 and P 40) had no apparent changes during stimulus series Ⅲ. Two dimensions topography recording displayed that the early components of SEPs originated from the contralateral hemisphere(C4); and the late components from the slight contralateral part of the central hemisphere extending to the frontal cortex. The amplitude values of SEPs at different time courses after 87.4 ms during stimulus series Ⅲ were significantly lower than those of stimulus series Ⅱ and Ⅳ. The inhibitory effect of EA of Hegu (LI 4) on MN-stimulation induced SEPs became obvious at 87.4 ms, peaked at 170 ms and lasted for 337.4 ms. The peak values of N 20 and P 40 and the PP values of N 20-P 40 from CZ (the parietal-central zone) and C4 (the contralateral vertex zone) had no significant differences among series Ⅱ, Ⅲ and Ⅳ (P>0.05); the peak values of N 80 from CZ also had no significant difference between series Ⅲ and Ⅳ (P>0.05); while the peak value of N 80 from CZ, and those of P 170 and N 280 and the PP values of N 80- P 170 and P 170- N 280 from CZ and C4 of series Ⅲ were all considerably lower than those of series Ⅱ and Ⅳ (P<0.05). The peak and PP values of various components of SEPs from CZ, and P 170, N 280,N 80-P 170 and P 170-N 280 from C4 of series Ⅳ were all lower than those of series Ⅱ. Conclusion:Both EA of Hegu (LI 4) and non-acupoint can inhibit the medium and late components of MN-stimulation induced SEPs, and the effect of EA of Hegu (LI 4) is strikingly superior to that of non-acupoint, suggesting a specific action of the acupoint, and the human cerebral cortex is involved in EA analgesia.关键词：电针;穴位;痛觉诱发脑电位888|0|2更新时间：2023-08-11
- 摘要：目的 :探讨针灸对红细胞免疫功能水平的影响。方法 :将 3 0只健康大鼠随机分为空白对照组、模型组和针灸组。针灸组每日行针刺及艾灸治疗 ,3 0min后 ,按游泳法复制大鼠气虚模型 ,连续重复 1 0天 ,次日各组采血抗凝 ,分别测定血液流变学指标、红细胞c3b受体花环率 (RBC .c3bRR)、红细胞免疫复合物花环率 (RBC .ICRR)。结果 :针灸组大鼠血液流变学指标均明显低于模型组 ,组间比较具有显著性差异 (P <0 0 1 ) ;模型组和针灸组的RBC .c3bRR均明显低于空白对照组 ,而RBC .ICRR明显高于空白对照组 (P <0 0 1 ) ;针灸组的RBC .c3bRR明显高于模型组 ,而RBC .ICRR明显低于模型组 (P <0 0 1 )。结论 :针灸对气虚大鼠的红细胞免疫功能低下具有一定的恢复作用Objective: To study the effect of acu-moxibustion on red blood cell immunologic function in rats with Qi-deficiency. Methods:30 Wistar rats were randomly and evenly divided into control group, model group and acu-moxibustion group. Qi-deficiency model was established by forcing the rat to swim continuously till it tended to sink spontaneously every day, ten days altogether. For rats of acu-moxibustion group, "Guanyuan"(CV 4), "Qihai"(CV 6) and bilateral "Zusanli"(ST 36) were punctured and stimulated electrically for 15 min, followed by performing scar-producing moxibustion for about 10 min. Whole blood viscosity (ηb) ratio, plasma viscosity (ηp) ratio, whole blood reduction viscosity ratio (RVR) and hematocrit (HCT) and erythrocyte electrophoresis time (EET), red blood cell c 3b receptor rosette rate (RBC.c 3bRR) and RBC immuno-complex rosette rate (RBC.ICRR) were detected and calculated before and after treatment respectively. Results: In comparison with control group, ηb and ηp ratios, RVR, HCT and EET of model group and acu-moxibustion increased significantly (P<0.05～0.01). Compared with model group, values of these 5 indexes in acu-moxibustion group lowered significantly (P<0.01). It indicates that acu-moxibustion can significantly improve blood rheology of Qi-deficiency rats. Compared with control group, RBC.ICRR values of model group and acu-moxibustion group increased significantly (P<0.01), while RBC.c 3bRR of the later two groups reduced evidently (P<0.01). Compared with model group, RBC.ICRR of acu-moxibustion group decreased evidently (P<0.01), while RBC.c 3bRR increased significantly (P<0.01), suggesting improvement of the cellular immunofunction.Conclusion: Acu-moxibustion can promote the restoration of the lowered red blood cell immunofunction in rats with Qi-deficiency.关键词：针灸;红细胞免疫功能;气虚1158|0|1更新时间：2023-08-11
- 摘要：目的 :探讨针刺减肥相关的细胞分子机制。方法 :采用RT PCR和NorthernBlot技术测定下丘脑神经肽Y(NPY)基因表达水平 ,放射免疫分析测定血浆和下丘脑NPY的含量 ,观察针刺治疗前后肥胖大鼠体重、Lee’s指数、体脂、血浆和下丘脑NPY的含量以及下丘脑NPY基因表达的变化。结果 :肥胖的大鼠体重、Lee’s指数、体脂、血浆和下丘脑NPY的含量以及下丘脑NPY基因表达均显著高于正常大鼠。针刺治疗取得良好减肥疗效的同时 ,肥胖大鼠血浆和下丘脑NPY含量以及下丘脑NPY基因表达水平明显回降。结论 :针刺对肥胖机体中枢和外周NPY水平的良性调整作用以及抑制下丘脑NPY基因过度表达可能是针刺减肥的细胞分子机制之一。关键词：针刺;肥胖;神经肽Y;下丘脑997|0|3更新时间：2023-08-11
- 摘要：目的 :通过测定亚急性衰老小鼠血清NO浓度、红细胞中SOD活力和血浆MDA的含量 ,进一步探讨NO与衰老的关系以及艾灸的延缓衰老作用。方法 :以D 半乳糖造成的亚急性衰老小鼠为实验对象 ,艾灸“大椎”、“命门”、“足三里”穴 ,治疗结束后测定NO浓度、SOD活力和MDA含量。结果 :模型组NO浓度与空白组比较明显下降 (P <0 0 5) ;各治疗组与模型组相比 ,NO浓度、SOD活力上升 ,MDA含量下降 ,具有显著性意义 (P <0 0 1 )。结论 :NO与衰老之间有一定的相关性 ,衰老时血清NO浓度降低 ;艾灸具有延缓衰老的作用Objective: To observe the effect of moxibustion on serum nitric oxide (NO) content in aging mice. Methods: A total of 50 Kunming mice were evenly randomized into control group, model group, "Zusanli"(ST 36) group, "Dazhui"(GB 14)-"Mingmen"(GV 4) group (DM-group) and "Zusanli"-"Dazhui"-"Mingmen" (ZDM) group. Subacute aging mouse model was established by subcutaneous injection of 5% D-galactose (0.5 mL/20 g body weight), once daily and continuously for 42 days. Suspended moxibustion was applied to ST-36, GB-14 and GV-4 for 2 min every acupoint, once daily, with 5 days being a therapeutic course, continuously for 5 courses. After finishing the treatment, blood samples were taken by extracting the eyeball for determining the contents of nitric oxide (NO) and malondialdehyde (MDA) as well as red blood cell superoxide dismutase (SOD) activity.Results: In comparison with control group, NO content and SOD activity decreased and MDA content increased significantly in model group (P<0.05～0.01); while in three moxibustion group, serum NO levels and SOD activity raised considerably and MDA lowered remarkably (P<0.01). It suggests that moxibustion can effectively raise NO level and SOD activity and lower MDA content in aging mice. In addition, in model group, both thymic index and spleen index reduced significantly compared with those of control group (P<0.01); after moxibustion, these two indexes increased evidently, being close to those of normal control group. Histological examination showed that the thymus and spleen of model group presented an atrophic state but those of three moxibustion groups were close to normal. Conclusion: Moxibustion can promote the increase of serum NO level and SOD activity and reduce MDA level in aging mice, that may be responsible for moxibustion induced aging-delaying action.关键词：艾灸;衰老;血清一氧化氮;超氧化物歧化酶;丙二醛1002|0|1更新时间：2023-08-11
- 摘要：目的 :研究针刺心俞、厥阴俞穴结合药物对急性缺血性脑卒中 (简称AIS)患者的心脑功能损害的保护作用。方法 :将 40例患者按入院的先后顺序随机分为两组 :心俞、厥阴俞穴结合药物组 2 0例 (治疗组 )及单纯药物组 2 0例 (对照组 ) ,观察治疗前后头颅CT脑梗塞体积、心电图、降钙素基因相关肽 (CGRP)、血栓素B2 (TXB2 )、6 酮 前列腺素 1a( 6 Keto PGF1a)以及神经功能缺损评分和临床疗效等指标的变化。结果 :与对照组比较 ,治疗组在下列指标上优于对照组 :脑梗塞体积(P <0 0 1 )、心电图 (P <0 0 1 )、CGRP(P <0 0 1 )、TXB2 (P <0 0 0 1 )、6 Keto PGF1a(P <0 0 1 )、神经功能缺损评分 (P <0 0 0 1 )、临床疗效 (P <0 0 5)。结论 :针刺心俞、厥阴俞穴对AIS患者的心脑功能损害有保护作用Objective:To study the protective effect of acupuncture combined with medication on cardiac and cerebral functions in patients suffering from acute ischemic stroke (AIS). Methods: 40 ischemic apoplectic patients were randomly divided into treatment group (n=20) which was treated by acupuncture of bilateral Xinshu (BL 15) and Jueyinshu (BL 14, once daily, ten sessions being a therapeutic course and 3 days' rest between two courses) combined with medication (20% mannitol, low molecular dextran, compound red sage root injection, persantine and aspirin) and control group (n=20) which was treated with simple medication (same medicines mentioned above). After 20 days' treatment, the therapeutic effect was evaluated. Changes of indices such as the volume of cerebral infarction, ECG-ST and T waves, heart rate (HR), plasma calcitonin gene-related peptide (CGRP), thromboxane B 2 (TXB 2) and 6-Keto-(prostaglandin) PGF1a contents, scores of neurological defect and clinical therapeutic effects were observed. Results: Following 2 courses of treatment, self-comparison of two groups showed that in comparison with pre-treatment, the cerebral infarction volume, HR and TXB 2 of treatment group were decreased significantly (P<0.05～0.01),while no striking changes were found in cerebral infarction volume, HR, ECG-ST and T-wave amplitude, plasma CGRP, TXB 2, and 6-Keto- PGF1a contents of control group (P>0.05). In addition, after treatment, the depressed ECG-ST and T-wave amplitudes of treatment group were elevated significantly compared with pre-treatment (P<0.05). Plasma CGRP concentrations of two groups both increased significantly (P<0.01～0.001), while the increased value of treatment group was evidently higher than that of control group (P<0.01). Compared with pre-treatment, plasma TXB 2 and 6-Keto-PGF1a of treatment and control groups increased and decreased significantly and separately (P<0.05). Following treatment, the scores for neurological defect of two groups both decreased significantly, while the decreased values of treatment group was significantly bigger than that of control group (P<0.001). The total effective rate (70%) of treatment group was considerably higher than that (20%) of control group (P<0.05). Conclusion: The protective effect of acupuncture plus medication on cardiac and cerebral functions in patients with ischemic stroke is significantly superior to that of simple medication.关键词：缺血性脑卒中;针刺疗法;药物疗法;心脑功能1081|0|1更新时间：2023-08-11
- 摘要：目的 :观察电针刺激对缺血再灌注心肌保护中IL 8的作用机制。方法 :48例行冠脉搭桥术病人随机分为针刺组和对照组。于术前、体外循环转流前、停转流 60min和术毕取右心房血测定IL 8、SOD、MDA。转流前、停转流 60min取右心耳心肌组织 ,电镜观察心肌超微结构变化。结果 :两组IL 8含量持续上升 ,针刺组停转流 60min、术毕含量显著低于对照组。SOD、MDA于转流前至术毕呈现下降趋势 ,其中针刺组转流前SOD较对照组显著升高 ,而MDA显著降低。电镜观察显示停转流 60min针刺组心肌细胞损伤程度明显小于对照组。结论 :针刺能抑制IL 8的释放 ,减少氧自由基的产生 ,减轻受氧自由基的攻击程度 ,从而对缺血再灌注心肌具有保护作用Objective: To observe the action of IL-8 in electroacupuncture (EA)-produced protective effect in patients undergoing coronary artery bypass grafting (CABG).Methods: Forty-eight patients undergoing CABG were randomized into EA group (n=24) and control group (n=24). In EA group, bilateral Neiguan (PC 6), Lieque (LU 7) and Yunmen (LU 2) were punctured and stimulated electrically with parameters of 3～4 Hz, 0.5～1 mA and duration of 20～30 min. Blood samples were taken from the right cardiac atrium before operation, before cardiopulmonary bypass (CPB), 60 min after CPB and at the end of operation in order to detect contents of IL-8, malondialdehyde (MDA) and superoxide dismutase (SOD). Myocardial tissues of the right atrium were taken before and 60 min after CPB in order to observe ultrastructural changes of myocytes. Results:Self-comparison between pre-operation, pre-CPB and post-CPB and post-operation in each group showed that the contents of IL-8 in both groups increased considerably 60 min following CPB and operation (P<0.05). IL-8 levels 60 min after CPB and at the end of operation in EA group were significantly lower than those of control group (P<0.05). The activity of SOD and the content of MDA before CPB in each group increased significantly compared with pre-operation and tended to decrease from 60 min after CPB to the end of operation (P<0.05). Comparison of two groups showed that SOD and MDA levels of EA group before CPB were significantly higher than those of control group (P<0.05). Electronmicroscopic observation showed that the myocardial cellular damage (swelling of mitochondria, myofibril breaking, etc.) was lighter in EA group than that in control group.Conclusion: EA can suppress the release of blood IL-8, which reduces the production of oxygen free radical and relieves its attack on myocytes, preventing the myocardium from ischemia-reperfusion injury.关键词：电针;心肌缺血再灌注损伤;白细胞介素-8;丙二醛;超氧化物歧化酶1140|0|3更新时间：2023-08-11
- 摘要：目的 :观察针刺对冠心病患者血瘀高凝状态中血栓长度 (VLT)、纤维蛋白原 (Fbg)、血小板聚集率 (PAGM)的影响。方法 :将 60例冠心病患者随机分为针药组、药物组各 3 0例 ,测定其治疗前后VLT、Fbg、PAGM水平。结果 :冠心病患者的VTL、Fbg、PAGM水平明显高于正常健康体检者 (P <0 0 1 ) ,针药组治疗后VTL、PAGM低于药物组 (P <0 0 5) ,且临床疗效优于药物组 (P<0 0 5)。结论 :针药并用可较好地改善冠心病患者血瘀高凝状态 ,提高冠心病的疗效 ,为临床治疗冠心病的有效方法之一Objective: To observe the effect of acupuncture plus medicines on the hypercoagulable state of blood in coronary heart disease (CHD) patients. Methods: A total of 60 CHD patients were randomized into acupuncture plus medicine group (n=30) and medication group (n=30). In addition, other 30 cases of normal people were attributed to control group. Main acupoints used were Neiguan (PC 6), Juque (CV 14), Tanzhong (CV 17), Xinshu (BL 15) and Jueyinshu (BL 14); and medicines employed were 1) 5% glucose 200 mL plus Danshen (red sage root) injection 20 mL; and 2) revised polarized liquid (10% glucose 500 mL, 10% potassiumichloride 10 mL and insulin 8 U for intravenous infusion, once daily, with 15 days being a therapeutic course; as well as enteric soluble Aspirin 50 mg (qid), Beitaloke 25 mg (bid), Catopril 12.5 mg (bid), etc. for oral administration. Acupuncture was given once daily, with 15 sessions being a therapeutic course. Vitro length of thrombus(VLT), fibrinogen (Fib) and platelet agglutination rate (PAGM) were detected separately. Results: After one course of treatment, of the 30 cases in medication group, 10 had marked improvement in clinical symptoms and ECG-ST, 12 were effective and 8 failed in the treatment, with the effective rate being 73.3%. In acupuncture plus medicine group, of the 30 cases, 13 had pronounced improvement in symptoms and ECG-ST, 15 were effective and 2 failed in the treatment, with the effective rate being 93.3%. The therapeutic effect of acupuncture plus medicine group was significantly superior to that of medication group (P<0.05). Before treatment, there were no significant differences between acupuncture plus medicine group and medication group in VLT, Fib and PAGM; following treatment, self-comparison of each group showed that all the 3 indexes decreased significantly in values (P<0.05～0.01), and comparison between two groups indicated that VLT and PAGM of acupuncture plus medicine group were evidently lower than those of medication group (P<0.05). Conclusion: Acupuncture combined with medicines may improve clinical symptoms of CHD patients and reduce their hypercoagulable state of blood.关键词：冠心病/针灸疗法;血栓长度;纤维蛋白原;血小板聚集率1055|0|0更新时间：2023-08-11
-
- 摘要：目的 :观察针刺对高脂血症患者血脂水平的改善情况。方法 :将 60例高脂血症患者随机分为治疗组和对照组 ,分别采取针刺及药物疗法。治疗结束后测定高脂血症各相关指标。结果 :针刺疗法可改善高脂血症血清中总胆固醇、甘油三脂、高密度脂蛋白和低密度脂蛋白的含量水平 ,与治疗前比较有显著性差异 (P <0 0 5) ,与对照组比较无显著差异。结论 :针刺的降脂作用与松龄血脉康胶囊相同 ,疗效明显 ,安全、经济、无毒副作用Objective: To observe the effect of acupuncture on blood lipid in hyperlipidemia patients.Methods: A total of 60 hyperlipidemia patients were evenly randomized into treatment group and control group. In treatment group, bilateral Zusanli (ST 36), Sanyinjiao (SP 6), Fenglong (ST 40) and Zhongwan (CV 12) were punctured with gauge-28 filiform needles that were manipulated with uniform reinforcing-reducing method continuously for a while, once every 4 min, 20 min altogether. The treatment was conducted once daily, with 10 sessions being a therapeutic course. After 2～3 days' rest, the next course of treatment was carried out. In control group, patients were ordered to take "Songling Xuemaikang" capsules, 3 pills every time, 3 times everyday, continuously for 30 days. Serum total cholesterol (TC) was determined by using cholesterol esterase/peroxidase enzymatic method, triglyceride (TG) detected using lipase glycerol kniase enzymatic method, serum high density lipoprotein (HDL) detected with phosphate tungstate acid (PTA)-Mg 2+ precipitation method, and low density lipoprotein (LDL) detected with polyvinyl sulfuric acid method.Results: Following 30 days' treatment, self-comparison between pre- and post-treatment in each group showed that serum TC, TG, and LDL levels in these two groups decreased significantly (P<0.05) , while serum HDL increased evidently (P<0.05). There were no significant differences between two groups in these indexes (P>0.05). It displays that both acupuncture and "Xuemaikang" capsule work well in lowering hyperlipidemia.Conclusion: Acupuncture therapy is an effective method for reducing blood lipid in hyperlipidemia patients.关键词：高脂血症;针刺873|0|4更新时间：2023-08-11
- 摘要：目的 :通过测定针刺 (或穴位电极刺激 )复合小剂量硬膜外麻醉胆囊切除术血糖含量的变化 ,推测针刺复合麻醉具有调整交感神经功能 ,减轻手术应激反应的作用。方法 :胆囊切除术共46例 ,其中A组 (针硬组 ) 1 6例 ,B组 (电硬组 ) 1 4例 ,C组 (对照组 ) 1 6例。每例于术前 1天 ,术中3 0min、术后 3天分别抽外周静脉血测定血糖含量。结果 :A、B两组血糖值术中比术前略升高 ,组内比较无显著差异 (P >0 .0 5)。C组 (对照组 )血糖值术中比术前升高明显 ,组内比较有极显著差异 (P <0 .0 1 )。A、B两组与C组术中血糖值比较有极显著差异 (P <0 .0 1 )。结论 :A组 (针硬组 )或B组 (电硬组 )在复合硬膜外麻醉中具有调整交感神经功能 ,减轻手术应激反应 ,使术中、术后血糖稳定等优点Objective: To observe the effect of acupuncture combined with extradural administration of anesthetic on blood sugar level in patients experiencing cholecystectomy for evaluating the regulative effect of acupuncture on sympathetic nerve activity. Methods: 46 cases of cholecystectomy patients were randomly divided into group A (n=16, electroacupuncture combined with extradural administration of small dose of analgesic-1.5% Lidocaine, etc.) and group B (n=14, transcutaneous electrical stimulation combined with extradural administration of small dose of analgesic) and control group (n=16, simple epidural administration of analgesic). In group A and B, bilateral Neiguan (PC 6) and Zusanli (ST 36) were used for EA (2/15 Hz, 2～3 mA) and transcutaneous electrical nerve stimulation (2/ 15 Hz, 13 mA). Blood sugar contents were determined 1 day before operation, during operation (30 minutes after anesthesia) and 3 days after operation in all the cases. Results: Results displayed that in group A and B, patients with excellent analgesic effects were found in 11 (68.8%) and 6 (42.9%) cases, with good analgesic effect found in 5 (31.2%) and 7 (50.0%) cases, and with poor analgesic effect in 0 and 1 (7.1%) case respectively. While in control group, those with good analgesic effect were found in 2 (12.5%) cases, with poor analgesic effect found in 12 (75.0%) cases, and 2 (12.5%) cases failed in the anesthetic effect. There were no significant changes of blood sugar levels in group A and B during and after operation in comparison with pre-operation, while in group C, blood sugar levels increased evidently during and after operation (P<0.01). Comparison among the 3 groups showed that during operation, blood sugar levels of group A and B were markedly lower than that of control group (P<0.01). Conclusion: Electroacupuncture or transcutaneous electrical nerve stimulation combined with extradural administration of small dose of analgesic has a good analgesic effect, and stabilize blood sugar level that may be closely associated with acupoint-stimulation induced decrease of sympathetic activity and stress response during operation.关键词：针刺复合硬膜外麻醉;胆囊切除术;血糖872|0|1更新时间：2023-08-11
- 摘要：目的 :观察固本通络电针法对糖尿病周围神经病变 (DPN)患者周围神经电生理的影响 ,为本疗法的疗效提供可靠的实验依据。方法 :84例DPN病人随机分为电针组和弥可保对照组 ,观察治疗前后神经传导速度 (NCV)、躯体感觉诱发电位 (SEP)、穴位皮肤痛阈 (PSP)的变化。结果 :电针组和对照组的NCV均有部分明显加快 (P <0 0 5) ,PSP显示了双相调整、趋于平衡的变化 ,电针组SEP的N1 波明显缩短 (P <0 0 5) ,在以上 3项指标的改善方面 ,电针组都明显优于对照组 (P<0 0 5,P <0 0 1 )。结论 :固本通络电针法可以改善DPN患者的神经功能 ,是治疗DPN的有效方法Objective:To observe the effect of acupuncture on peripheral nuroelectrophysiological changes in diabetic peripheral neuropathy(DPN)patients in order to provide experimental basis for this therapy. Methods:Eighty-four cases of diabetes patients with DPN were randomly divided into acupuncture group (n=48) and simple medication (Methylcobalamin) control group (n=36). Acupoint groups used were 1) Qihai (CV 6), Guanyuan (CV 4), Fenglong (ST 40) and Sanyinjiao (SP 9); 2) Pishu (BL 20), Shenshu (BL 23), Huantiao (GB 30) and Feiyang (BL 58), which were punctured with reinforcing method or even needling method, and some of which located in the nerve injured area were also stimulated with electroacupuncture (EA). Additionally, patients of two groups were also treated by oral administration of Gliclazide, Dimethyldiguanide, Phenformin and "Xiaoke Wan"(Pills for treating diabetes). The treatment was given once every other day, continuously for 2 months. Changes of nerve conduct velocity (NCV), somesthetic evoked potential(SEP),and the pain threshold (PT) of the acupoint-skin before and after the treatment were observed. Results: Following acupuncture treatment, motor NCV (right median nerve and the left ulnar nerve) and sensory NCV (bilateral median nerve and the left peroneal nerve) in acupuncture group increased evidently (P< 0.05); and motor NCV of acupuncture group is significantly higher than that of control group (P< 0.05). In control group, NCV of the left peroneal nerve (motor N) and the right median nerve (sensory N) also increased significantly (P<0.05). Sensory NCV of the left median nerve and peroneal nerve of acupuncture group were also markedly higher than those of control group (P<0.05). After treatment, only the latency of N 1 of SEP of the left median nerve shortened evidently (P<0.05), while that of control group prolonged, showing that the effect of acupuncture is superior to that of medication (P<0.05). Following treatment, the PT values of Zusanli (ST 36) and Taichong (LR 3) in two groups increased or lowered significantly in comparison with those of pre-treatment (P<0.05～ 0.001). Conclusion:Acupuncture treatment can improve the nerve function of DPN patients,and is thus a valid method for relieving DPN.关键词：糖尿病周围神经病变;电针;神经传导速度;躯体感觉诱发电位;痛阈956|0|4更新时间：2023-08-11
- 摘要：随着人口老龄化 ,心脑血管疾患日益增多 ,心脑等脏器疾病是造成临床致死、致残的主要原因 ,目前仍采用综合防治方法。针刺对心脑缺血再灌注有防治作用 ,但其作用机制还不完全清楚。本文汇总了近几年从基因水平研究针刺对缺血再灌注的作用的文献 ,主要描述了针刺对缺血再灌注后心脑组织细胞的基因变化的影响 ,包括热休克蛋白、原癌基因、细胞因子、Bcl 2等 ,并描述了作用机制 ,从而概括地说明针刺在缺血再灌注后损伤中发挥的作用In the present paper, the authors review recent advances of researches on the effect of acupuncture on cellular gene expression in ischemia-reperfusion (IR) heart and brain tissues. Acupuncture has a good protective effect on the IR injured tissues of the heart and brain, but the underlying mechanisms have not been clarified completely up to now. This paper gathers some literature and tries to elucidate the favorable effect of acupuncture treatment from following aspects: 1) Effect of acupuncture on heat shock protein: Heat shock protein (HSP) family including HSP90, HSP70, HSP72 and HSP60 may increase in gene expression in response to environmental stimulation, simultaneously, the cells increase their endurance upon new stimulation. Acupuncture stimulation can up-regulate expression of myocardial HSP mRNA and neurons of the cerebral ischemic penumbra. 2) Effect of acupuncture on c-fos expression in IR brain tissue: In the early period of cerebral ischemia, increase of c-fos mRNA may be helpful to the recovery and re-encoding of protein transcription. After acupuncture stimulation, c-fos mRNA expression in some ischemic cerebral regions is increased further; on the other hand, acupuncture can also suppress the over-expression of c-fos, which may contribute to the protective action of acupuncture on ischemic neurons. 3) Effect of acupuncture on cerebral (superoxide dismutase) SOD: Acupuncture can raise SOD activity of the ischemic cerebral tissues to resist free radicals-induced injury. 4) Effect of acupuncture on cytokines: Acupuncture stimulation can raise IL-2 level. Cerebral IR may induce expression of IL-1Ra mRNA of the cerebral cortex, following acupuncture, its expression increases further. 5) Effect of acupuncture on Bcl-2 expression: EA may up-regulate expression of Bcl-2 in the IR cerebral tissue to resist apoptosis. 6) In addition, acupuncture may also promote expression of nerve growth factor (NGF).关键词：针刺;缺血再灌注;基因表达;细胞因子1019|0|0更新时间：2023-08-11
- 摘要：本文就井穴的实验研究最新进展进行文献整理 ,从生化免疫、血液流变、电生理、微循环等方面进行综述 ,认为针灸井穴对急症有显著疗效。但目前对井穴治病范围的研究仍非常局限 ,有待进一步深入研究探讨In this paper, the authors review recent development of studies on acupuncture and moxibustion of twelve Jing-points for treatment of different diseases. 1) Regarding the biochemical field, pricking blood of the twelve Jing-points can prevent the ion-pumps of cellular membrane of the ischemic cerebral tissue from failure; suppress cerebral ischemia-induced increase of calmodulin activity in the brain tissues, and raise partial pressure of oxygen (PO 2) of the ischemic cerebral tissue to reduce the anoxic state of the brain. 2) Concerning the blood rheology and hemodynamics, pricking blood of the twelve Jing-points could facilitate blood flow of the ischemic cerebral tissue, and improve intra-cranial blood flow velocity in stroke patients. 3) In regarding to electrophysiological aspect, pricking blood of the twelve Jing-points could accelerate stroke patients' heart rate and improve their mental activity. 4) Laser radiation of Jing-points could improve patients' nail-fold microcirculation. At present, acupuncture of twelve Jing-points is still limited in the scope of treatment of diseases and further researches are necessary.关键词：十二井穴;针灸治疗1045|0|0更新时间：2023-08-11
- 摘要：本文通过整理文献对针灸治疗腰椎间盘突出症的选穴、操作手法 ,以及配合牵引、推拿、中药的综合疗法作了总结 ,认为针灸治疗本病的疗效是肯定的 ,以夹脊穴为主治疗腰椎间盘突出症是近期的主流 ,可达到消肿、消炎 ,甚至髓核回纳的目的。对急性期病人 ,针灸可以改变腰椎间盘及椎管的位置和形态 ;对已形成退变的椎间盘 ,针灸仍然可以缓解其临床症状。动物实验证明针刺可促进大鼠神经纤维轴突内轴浆运输 ;多次电针可促进大鼠弓状核内POMCmRNA表达的不断增多。在治疗时间上 ,腰椎间盘突出症为越早治疗效果越好In the present paper, the author reviews recent development of acupuncture treatment of prolapse of intervertebral disc from 1) acupuncture treatment including electroacupuncture (EA) therapy, wrist-ankle therapy, combined therapies of EA and cupping, or acupuncture and moxibustion, etc., by choosing body acupoints, scalp-points, abdominal acupoints, etc.; 2) combined treatment of acupuncture and lumbar vertebral traction; 3) combined treatment of acupuncture and massage; and 4) animal experimental study and clinical research on the action mechanism of acupuncture. In regard to acupoint selection, lumbar Jiaji (EX-B 2) is used very often; additionally, some back-shu points and those of the lower limbs are also frequently used in clinic. Results indicate that acupuncture therapy works well in improving clinical symptoms of patients with prolapse of intervertebral disc. Animal experiments display that in nerve-root compression model, acupuncture can relieve demyelination of the compressed nerve fibers and reduce edema of nerve tissue. In sciatica rat model, acupuncture stimulation possesses analgesic effect for which acupuncture induced up-regulation of the expression of prooppiomelanocortin (POMC) of the arcuate nuclei of the hypothalamus may be responsible. CT examination suggests that after acupuncture treatment, the prolapsed pulpiform nucleus recover its normal position, which is identical to the alleviation of clinical symptoms. Ultrasonic examination shows that acupuncture and massage can widen the internal diameter of the vertebral canal and change the position relationship between the prolapsed lumbar intervertebral disc and the compressed nerve root.关键词：腰椎间盘突出症;针灸治疗937|0|0更新时间：2023-08-11
- 地址：北京市东城区东直门内南小街16号 邮编：100700
- 联系电话：010-64089344,84014607 Email：zcyjbjb@vip.163.com
- 技术支持由北京北大方正电子有限公司提供 京ICP备05017590号-8
京公网安备11010802020460号 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
0