最新刊期
2004年第2期
- 摘要：目的 :探讨针刺对缺血性脑损伤保护作用的生化机制。方法 :采用热凝闭大鼠大脑中动脉致局灶性脑缺血模型 ,研究缺血 2周和 5周后缺血区皮层突触素P3 8和GAP 43的变化规律和针刺对其影响。结果 :脑缺血 2周及 5周组大鼠脑缺血区P3 8表达比假手术组显著下降 (P <0 .0 5) ;缺血 +电针 2周组与缺血 2周组相比突触素P3 8表达并无显著性差异 (P >0 .0 5) ;缺血 +电针 5周组与缺血 +电针 2周组和脑缺血 5周组相比 ,突触素P3 8表达明显增加 (P <0 .0 5) ;但仍明显低于假手术 5周组 (P <0 .0 5)。缺血 2周组大鼠在缺血区周围GAP 43表达与假手术组相比增加明显 (P <0 .0 5) ,而缺血 5周组与假手术组相比无显著性差异 (P >0 .0 5) ;缺血 +电针 2周组大鼠脑片GAP 43表达与假手术组相比显著增加 (P <0 .0 5) ,而与缺血 2周组相比无差异 (P >0 .0 5) ;缺血 +电针 5周组大鼠脑片GAP 43表达与假手术 5周组相比有显著性差异 (P <0 .0 5)。结论 :针刺可以通过提高突触素和GAP 43在缺血中心区周围皮层的表达 ,保护缺血性脑损伤 ,并可能与大脑可塑性的形成有一定的关系Objective: To explore the underlying mechanisms of acupuncture in preventing the cerebral tissue from ischemic injury. Methods: 35 SD rats were randomized into sham operation 2 week (SO 2W) group (n=6), SO 5W group (n=6), cerebro ischemia 2 week (CI 2W) group (n=5), CI 5W group (n=6), CI 2W+electroacupuncture(EA) group (n=6) and CI 5W+EA group (n=6). Cerebral ischemia model was established by middle cerebral artery heat occlusion method. EA (3～ 5 V, 5～14 Hz, dense sparse waves) was applied to “Dazhui"(GV 14) for 30 min, once daily and continuously for 2 weeks. Cerebral integral neuronal synaptic vesicle membrane protein synaptophysin (P38) and growth associated protein (GAP) 43 immunoreaction of the cerebral tissue sections were displayed by using immunohistochemical technique and the immunoactivity (corrected optical density, COD) was detected by using a spectrometer system. Results: Compared with SO 2W group, COD values of P38 of CI 2W and CI 2W+EA groups were significantly lower (P<0.05); and compared with SO 5W group, P38 COD of CI 5W and CI 5W+EA groups were also significantly lower (P <0.05). Comparison between CI 2W and CI 5W groups and between CI 2W+EA and CI 5W+EA groups indicated that P38 COD values of CI 5W group and CI 5W+EA group were remarkably higher than those of CI 2W and CI 2W+EA groups separately (P<0.05), and that of CI 5W+EA group was significantly higher than that of CI 5W group (P<0.05). Compared with SO 2W group, GAP 43 COD values of both CI 2W and CI 2W+EA groups were strikingly higher (P<0.05); and compared with SO 5W group, that of CI 5W+EA group was significantly higher. Comparison between CI 2W and CI 5W groups indicated that GAP 43 COD value of CI 5W group was remarkably lower than that of CI 2W group. No significant difference was found between CI 2W+EA and CI 5W+EA groups in GAP 43 COD values (P>0.05). It showed that after cerebral ischemia, P38 expression was downregulated markedly and GAP 43 expression upregulated, and EA could eliminate ischemia induced downregulation of P38 expression and prolong ischemia induced upregulation of GAP 43 expression. Conclusion: EA can upregulate expression of cerebral P38 and prolong the upregulation of GAP 43 expression in the cerebral ischemia rats.关键词：脑缺血;电针;突触素P38;GAP-431111|0|9更新时间：2023-08-11
- 摘要：目的 :观察针刺对脑缺血小鼠脑组织胶质纤维蛋白的影响。方法 :选用NIH雄性小鼠3 8只 ,随机分为正常组 (n =10 )、假手术组 (n =10 )、模型组 (n =8)、针刺组 (n =10 )。针刺“百会”、“神庭”、“率谷”、“脑户”穴 ,1天 1次 ,共 10次。用免疫组化法观测胶质纤维酸性蛋白 (GFAP)的表达。结果 :模型组海马结构中GFAP免疫组化染色阳性反应普遍增强 ,正常组和假手术组的阳性反应一致 ,而针刺组的阳性反应介于模型和正常鼠之间。结论 :针刺对小鼠学习记忆能力的改变与使GFAP阳性反应数减少有关Objective: To observe the effect of acupuncture on cerebral glial fibrillary acidic protein (GFAP) expression in cerebral ischemia rats. Methods: 38 NIH male mice were randomly divided into control group (n=10), sham operation group (n=10), model group (n=8) and acupuncture group (n=10). Cerebral ischemia model was established by occlusion of the bilateral common carotid arteries. “Baihui"(GV 20), “Shenting"(GV 24), “Shuaigu"(GB 8) and “Naohu"(GV 17) were punctured once daily, 10 times all together. GFAP expression was assayed with immunohistochemical technique. Results: GFAP immunoreaction (IR) positive neurons of the radiation layer of CA1 area in model group were significantly more than those of control group and sham operation group (P< 0.01). In comparison with model group, GFAP IR positive neurons of hippocampal radiation layer in acupuncture group were strikingly fewer (P<0.01), while no significant differences were found between four groups in IR positive neurons of lacuma molecular layer and hilus region of dentate gyrus of hippocampus (P>0.05). Conclusion: Acupuncture can downregulate cerebral ischemia induced stronger expression of GFAP in CA1 region of hippocampus.关键词：脑缺血;胶质纤维酸性蛋白表达;针刺1063|0|0更新时间：2023-08-11
- 摘要：目的 :观察头穴针刺对缺血再灌注大鼠脑内肿瘤坏死因子α(TNF αmRNA)表达的影响 ,探讨针刺治疗缺血性脑损伤的可能机制。方法 :采用大鼠局灶性脑缺血再灌注模型 ,应用原位杂交及HE染色方法观察脑缺血再灌注时TNF αmRNA的变化及缺血脑组织病理变化 ,以及针刺对其影响。结果 :假手术组大鼠TNF αmRNA在皮层、纹状体呈基础水平表达 ,脑缺血再灌注后12hrTNF αmRNA表达增强 (P <0 .0 5) ,针刺可明显抑制皮层、纹状体内TNF αmRNA的表达(P <0 .0 5) ,组织学中其神经组织变性、坏死及血管炎性反应也明显减轻。结论 :针刺对缺血性脑损伤的保护作用机制可能与针刺抑制脑内TNF αmRNA的表达有关Objective: To observe the effect of acupuncture on the expression of tumor necrosis factor alpha(TNF α) in focal cerebral ischemia reperfusion rats for researching the underlying mechanism of acupuncture in reducing cerebral ischemic injury. Methods: A total of 15 SD rats were randomly and evenly divided into sham operation, acupuncture and model groups. Cerebral ischemia reperfusion model was established by occlusion of the middle cerebral artery (in accordance with Koizumi's method) for 2 hr and reperfusion for 12 hr. In situ hybridization was used to detect the expression of TNF α mRNA of the sliced brain tissues. The histologic changes of the brain tissue was also observed under microscope after staining with hematoxylin eosin (HE) method. Results: Compared with sham operation group, the average optical density values of the cerebral cortex and striate body tissues in model group and acupuncture group increased significantly (P<0.05), suggesting over expression of TNF α mRNA after cerebral ischemia reperfusion. In comparison with model group, the optical density values of the cerebral cortex and striate body in acupuncture group decreased considerably (P< 0.05), suggesting that acupuncture can suppress the over expression of TNF α mRNA induced by cerebral ischemia reperfusion. Under microscope, the state of neuronal degeneration (vacuolation, etc.) and necrosis, leukocyte aggregation at the vascular wall and leukocyte infilitration around the blood vessels was severer in model group and lighter in acupuncture group. Conclusion: Scalp acupuncture can downregulate cerebral ischemia reperfusion induced over expression of TNF α mRNA, which may contribute to the effect of acupuncture in protecting cerebral neurons from ischemia reperfusion injury.关键词：头针;脑缺血再灌注;肿瘤坏死因子α1022|0|3更新时间：2023-08-11
- 摘要：目的 :研究头皮针对脑缺血模型大鼠神经生长因子 (NGF)的影响 ,并与电针组比较。方法 :线栓法制备大鼠脑缺血模型 ,脑组织甲醛固定切片 ,运用免疫组化方法检测各组神经生长因子的变化情况。结果 :术后 6hr与术后 15天组内神经功能评分比较 ,模型组无明显差异 (P >0 .0 5) ,电针组与针刺组有显著性差异 (P <0 .0 1) ;组间 15天时比较 ,电针组与针刺组无明显差异(P >0 .0 5) ,两组与模型组比较均有显著性差异 (P <0 .0 1)。在第 15天时神经生长因子免疫组化染色比较 ,电针组与模型组有明显差异 (P <0 .0 5) ,针刺组与模型组有显著性差异 (P <0 .0 1) ,针刺组与电针组有明显差异 (P <0 .0 5)。结论 :头皮针可促进神经生长因子产生并延长神经生长因子产生时限 ,这可能是头皮针减轻脑缺血损伤并促使肢体功能恢复的机理之一Objective: To study the effect of scalp acupuncture on cerebral nerve growth factor (NGF) expression in cerebral ischemia rats. Methods: A total of 50 Wistar rats were evenly randomized into control, sham operation, model, electroacupuncture (EA) and hand acupuncture groups. Cerebral ischemia model was established by occlusion of the right middle cerebral artery with a nylon thread embolus. “Baihui"(GV 20) and “Qubin"(GB 7) were punctured and stimulated with an EA stimulator (EA group) and hand manipulation (hand acupuncture). The treatment was given once daily and continuously for 15 days. The brain tissue samples were fixed with formaldehyde, sliced up, and then stained with immunohistochemical technique for observing NGF expression. Results: Auto comparison between 6 hr and 15 d after operation in neurological defect scores of model group showed no significant difference (P>0.05), but in both EA and hand acupuncture groups, the neurological defect scores decreased apparently after treatment (P<0.01). There were notable differences between EA group and model group, and between hand acupuncture group and model group in the score (P< 0.01), but no distinct difference was found between EA group and hand acupuncture group in the score (P>0.05). It showed a favorable effect of acupuncture in improving cerebral ischemia induced neurological dysfunction. Immunohistochemical staining of the cerebral cortex tissue displayed that NGF immunoreaction (IR) positive neurons (+) appeared in only one animal of model group and no IR positive neurons were found in control and sham operation group. The mild (+), moderate () and strong () IR positive neurons in EA and hand acupuncture groups were found in 7 and 1, 2 and 7, 1 and 2 animals respectively. Rank test showed that the IR density of both EA and hand acupuncture group was stronger than that of model group (P<0.05～0.01) and the effect of hand acupuncture group was significantly superior to that of EA group (P<0.05). Conclusion: Scalp acupuncture can improve neurological function and upregulate the expression of cortical NGF in cerebral ischemia rats.关键词：头皮针;脑缺血;神经生长因子978|0|2更新时间：2023-08-11
- 摘要：目的 :运用自由基学说和胆碱能学说 ,探讨针刺对拟阿耳茨海默氏病 (AD)大鼠大脑皮质及海马抗氧化能力和胆碱能系统功能的影响。方法 :应用迷宫试验观测针刺对拟AD大鼠学习记忆能力的影响 ;并应用分光光度法测定大鼠大脑皮质中丙二醛 (MDA)含量及谷胱甘肽过氧化物酶 (GSH Px)、超氧化物歧化酶 (SOD)活性变化 ,应用巯基比色分析法、免疫组化方法测定大鼠海马区乙酰胆碱酯酶 (AChE)和胆碱乙酰化酶 (ChAT)活性变化。结果 :迷宫学习记忆能力测试表明 ,针刺组大鼠成绩好于模型组 (P <0 .0 1) ;针刺组大鼠大脑皮质中MDA含量比模型组显著降低(P <0 .0 1) ,而SOD和GSH Px活性明显升高 (P <0 .0 1) ;在AChE和ChAT测定中 ,针刺组比模型组大鼠AChE、ChAT活性明显增强 (P <0 .0 1)。结论 :针刺可增强脑内抗氧化能力 ,减轻脑内自由基对神经元的损伤 ,从而改善AD的病理反应 ,提高中枢胆碱能系统功能 ,缓解拟AD大鼠的学习记忆障碍Objective: To study the effects of acupuncture on antioxidative ability and cholinergic nervous system activity of the cerebral cortex and hippocampus in experimental Alzheimer's disease(AD)rats. Methods: 53 SD rats were randomized into control (n=15), model (n=13), electroacupuncture (EA, n=13) and rehabilitation (n=12) groups. AD model was established by intraperitoneal injection of AlCl 3 (49.4 mg/kg/day, continuously for 30 days). EA (dense sparse waves, 30 Hz) was applied to “Baihui"(GV 20), “Dazhui"(GV 14), “Shenshu"(BL 23) and “Xinshu"(BL 15) for 10 min, once every other day, continuously for 40 days. Rats of rehabilitation group were fed with Piracetum (0.32 g/kg), once daily. AD rats' learning memory ability was tested with maze method, and cerebral malondaldehyde (MDA) content, superoxide dismutase (SOD) and glutathione (GSH) Px activity were detected with biochemical methods, hippocampal AChE and ChAT activities were assayed with immunohistochemical technique. Results: The AD rats' learning memory ability in acupuncture group was significantly better than that of model group and rehabilitation group(P<0.01, 0.05). Compared with control group, cerebral MDA level of model group increased significantly, cerebral GSH Px and SOD activities, and hippocampal AChE and ChAT activities of model group lowered considerably (P<0.01); while in comparison with model group, cerebral MDA activity of rehabilitation and EA groups decreased significantly (P<0.05, 0.01) and the effect of EA was significantly superior to that of Piracetum (P<0.05). Compared with model group, GSH Px, SOD, AChE and ChAT activities raised remarkably and the effect of EA was also significantly superior to that of Piracetum (P<0.05, 0.01). Conclusion: Acupuncture can increase the antioxidative ability and reduce free radical mediated neuronal damage in the brain, which may contribute to its action in improving AD rats' learning memory ability.关键词：电针;阿耳茨海默氏病;自由基;胆碱能系统892|0|12更新时间：2023-08-11
- 摘要：目的 :观察电针“百会”穴对正常大鼠学习记忆能力的影响 ,这一影响和fos蛋白在海马区的表达有无相关性。方法 :利用智能Morris水迷宫检测大鼠的学习记忆能力 ,利用免疫组化方法 ,结合病理图像分析仪检测fos蛋白的表达情况。结果 :连续电针“百会”穴 3天后 ,实验组大鼠在智能Morris水迷宫内找到平台的时间和游泳的路程与对照组相比均缩短 (P <0 0 5) ,海马区fos蛋白大量表达 ,与对照组比较 ,差异显著 (P <0 0 1)。结论 :针刺“百会”可以提高大鼠记忆保持能力 ,其作用和海马区神经细胞原癌基因转录有关Objective:To investigate the influence of electroacupuncture(EA) on memory retention and the correlation between the effect of EA and the expression of fos protein in the rat hippocampus. Methods: 30 Wistar male rats were randomly divided into control group (n=10) and EA group (n=20). Intelligent Morris water maze place test was used to check the memory behavior reactions of two groups. EA (150 Hz, 2.0 mA, continuous waves) was applied to “Baihui"(GV 20) for 20 min, once daily, continuously for 3 days. The expression of c fos protein of sections of the hypothalamus tissue was assayed by immunohistochemical method. Results: Compared with control group, both the swimming distance and time that the rats found the flat of the water maze in EA group were significantly shorter (P<0 05), suggesting that EA can significantly improve the rats' memory. In addition, the c fos immunoreaction positive cells of the hypothalamic region (particularly the dentate gyrus) of EA group were considerably more than those of control group (P<0 01), suggesting that EA can upregulate expression of c fos protein in the hippocampus. Conclusion: EA can enhance the rats' memory, which may be related to the upregulation of c fos gene expression.关键词：记忆;电针;百会;fos蛋白926|0|7更新时间：2023-08-11
- 摘要：目的 :观察“心俞” “厥阴俞”贴敷强心卡帖对实验性动脉粥样硬化家兔血管结构、高血脂和高胆固醇血症的影响。方法 :大耳白家兔共 48只 ,随机分为正常对照组 (n =6) ,模型组 (n =13 ) ,2 .5月实验组 (n =19)和 3 .5月实验组 (n =10 )。动脉粥样硬化模型用高脂、高胆固醇饲料喂养 2～ 3个月造成。测量股动脉平均血压 (MAP) ,显微镜下测量主动脉、腹主动脉及心肌血管中外膜及内膜的厚度 ;观察心脏大、中小冠脉血管分支堵塞情况 ;生化用酶法测定血清甘油三脂 (TG)、总胆固醇 (TCh)、高密度脂蛋白胆固醇 (HDL C)、低密度脂蛋白胆固醇 (LDL C)的含量。喂养上述特化饲料前两周 ,开始将强心帖贴敷于“心俞” “厥阴俞”、心前区 ,共贴敷 2 .5～ 3 .5个月。结果 :①对照组、模型组、实验组 (3 .5月 )MAP值相比组间虽无显著差异 ,但实验组的值低于模型组的趋势还是比较明确的 ;②贴敷强心帖 2 .5个月时 ,实验组主动脉、腹主动脉内膜的厚度低于模型组 ;实验组血清LDL C含量略低于模型组 ;③贴敷 3 .5个月时 ,实验组腹主动脉和心肌冠脉血管内膜均低于模型组 ,对照组心肌冠脉血管分支的结构绝大多数为正常 ,模型组半数或半数以上发生血管严重堵塞 ,而实验组半数以上为轻度堵塞 ;④贴敷 3 .5个月时 ,实验组血清TG、Objective: To observe the effect of application of “Qiang Xin Tie" (Plaster for Strengthening Cardiac Performance) at “Xinshu"(BL 15) and “Jueyinshu"(BL 14) on vascular structure, serum high blood lipid and high cholesterol levels in artherolerosis rabbits. Methods: 48 healthy rabbits were randomly divided into control group (n=6), model group (n=13), 2.5 month (2.5 M, n=19) and 3.5 month (3.5 M, n=10) groups. Artherolerosis model was established by feeding the animal with specialized forage (12% yolk powder, 7% pig fat and 0.7% cholesterol and common forage) for 2 and 3 months respectively. Mean arterial pressure (MAP) of the femoral artery, the thickness of the endangium, tunicae media plus externa of the aorta, abdominal aorta and the endangium of the coronary vessels, the blockage state of the coronary vessels were observed and detected under microscope (micrometer). Serum triglyceride (TG), total cholesterol (TCh), high density lipoprotein cholesterol (HDL C) and low density lipoprotein cholesterol (LDL C) contents were assayed by zymochemical techniques. Two weeks before feeding the animals with the specialized forage, “Qiang Xin Tie" was applied to bilateral “Xinshu"(BL 15), “Jueyinshu"(BL 14) and the precordial region and replaced by a new one once every day or every other day, continuously for 2.5～3.5 months. Results: ① Comparison among control, model and 3.5 M groups showed that MAP of 3.5 M group was lower than that of model group (148.00±23 mmHg vs 159.71±14.07) and was close to that (145.00±17.61 mmHg) of control group. ② In 2.5 M group, the intimal thickness values of both aorta and the abdominal aorta and serum LDL C were lower than those of model group in spite of no statistical difference. ③ In 3.5 M group, the intimal thickness values of the abdominal aorta and the coronary vessels (larger, middle and smaller branches) were lower than those of model group; majority of the coronary vessels (94.4%) of control group were normal in the structure, 50.0% ～71.4% vessels had severe block, while 44.4%～61.1% in 3.5 M group had light block in the intravascular lumen. ④ In 3.5 M group, serum TG, TCh and LDL C were significantly lower than those of model group (P<0.05～0.01) and 2.5 M group (P<0.05～0.001). No significant changes were found in serum HDL C level. Conclusion: Acupoint application of “Qiang Xin Tie" may check the developing process of artherolerosis to a certain degree, lower blood pressure and blood lipid in the experimental artherolerosis rabbits.关键词：动脉粥样硬化/穴位贴敷疗法;高血脂;血管内膜1148|0|3更新时间：2023-08-11
- 摘要：目的 :观察电针“内关”对内毒素休克时肝功能的保护作用和肝组织一氧化氮合酶(NOS)表达的影响。方法 :静脉注射内毒素 (LPS) 1.5mg/kg、腹腔注射D 氨基半乳糖 (D GalN)10 0mg/kg造成内毒素休克模型 ,用电针“内关”和氨基胍 (AG)作对照干预处理 ,取血清检测肝功四项 ,用免疫组化方法检测诱生型一氧化氮合酶 (iNOS)、内皮型一氧化氮合酶 (eNOS)在肝组织内的表达。结果 :电针“内关”可以使肝功四项中ALT、AST、LDH值显著下降 ,使肝内iNOS表达减弱、eNOS表达增强 ,肝损害减轻。结论 :电针“内关”可以减轻内毒素休克造成的肝损害 ,这种保护作用可能是通过调节肝组织NOS表达而产生的Objective: To observe the effect of electroacupuncture (EA) of “Neiguan" (PC 6) on acute liver injury and liver NOS expression in endotoxin shock rats. Methods: 50 SD rats were randomly and evenly divided into control, model, Aminoguanidine Hemisulfate (AG), EA and EA+AG groups. Endotoxin shock model was induced by intravenous injection of Lipopolysaccharide (LPS, 1.5 mg/kg) and intraperitoneal injection of D galactosamine (D GalN, 100 mg/kg). The expression of iNOS and eNOS was measured by using immunohistochemical method. Serum alanine aminotransferase (ALT), aspartic acid transferase (AST), albumin/globulin and lactic dehydrogenase (LDH) contents were detected with a full automatic biological analyzer. EA (2～14 Hz, 1 mA) was applied to bilateral “Neiguan"(PC 6) for 150 min. AG, a selective inhibitor of iNOS was given to the rats intraperitoneally. Results: In comparison with control group, serum ALT, AST and LDH levels of model, AG, EA and EA+AG groups increased significantly from 30 min on after injection of LPS and D GalN (P<0.05～0.01), suggesting an injury of the liver tissues. Compared with model group, serum ALT, AST and LDH levels of EA and EA+AG groups decreased considerably from 60 min (ALT and AST) or 30 min (LDH) on after EA. No significant differences were found between model group and AG group in ALT, AST and LDH levels, and no apparent changes were found in serum A/G values in the five groups (P>0.05). Immunohistochemical staining displayed that the ratios of eNOS immunoreaction (IR) positive hepatocytes/the observed total hepatocytes of model, AG, EA and EA+AG groups were significantly lower than that of control group (P<0.05～0.01), and that of EA group was considerably higher than those of AG and model groups (P<0.01). While the ratios of iNOS IR positive hepatocytes of model, AG, EA and EA+AG groups were significantly higher than that of control group, and those of AG, EA and EA+AG groups were significantly lower than that of model group (P<0.05～0.01). Compared with AG group, the ratios of iNOS positive cells of EA and EA+AG groups were strikingly higher (P<0.05～0.01). It indicated that after liver injury, iNOS expression was upregulated and eNOS expression downregulated, AG had a suppressive effect on both iNOS (stronger in the effect) and eNOS (weaker), and EA could downregulate iNOS expression and upregulate eNOS expression. Conclusion: EA of Neiguan Point can reduce D GalN LPS induced acute hepatocyte injury probably by way of adjusting NOS protein expression.关键词：内关/电针;肝损害/内毒素休克;一氧化氮合酶982|0|0更新时间：2023-08-11
- 摘要：目的 :研究针灸对小鼠骨髓细胞微核的影响。方法 :将实验动物分为正常对照组 ,阳性对照Ⅰ、Ⅱ组 ,针灸预防Ⅰ、Ⅱ、Ⅲ、Ⅳ组 ,针灸治疗Ⅰ、Ⅱ组。针刺小鼠“足三里” ,艾灸“关元”穴 ,检测小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率的变化。结果 :阳性对照组与正常对照组比较 ,小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率明显升高。针灸预防Ⅰ、Ⅱ、Ⅲ、Ⅳ组 ,针灸治疗Ⅰ组与阳性对照Ⅰ组比较 ,针灸治疗Ⅱ组与阳性对照Ⅱ组比较 ,各指标明显降低 (P <0 0 0 1)。结论 :针灸可拮抗由环磷酰胺诱导的小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率的升高Objective: To observe the effect of acupuncture and moxibustion (acumoxi) on micronucleus formation of bone marrow polychromatic erythroblasts and nucleated cells.Methods:72 Kunming mice were randomly and evenly divided into normal control group, positive control groupⅠ and Ⅱ; prevention groupⅠ, Ⅱ, Ⅲ and Ⅳ; and treatment groupⅠand Ⅱ.In the later 8 groups, all the mice were given (i.p.) with cyclophosphamide (CP, 50 mg/kg) for inducing micronucleus formation, after one day (1 d)'s, 2 ds', 4ds' and 10 ds' treatment in prevention groupⅠ, Ⅱ, Ⅲ and Ⅳ; 24 hr and 48 hr before killing the rats in model group Ⅰ and Ⅱ; and before treatment in treatment groupⅠand Ⅱ. Bilateral “Zusanli"(ST 36) and “Guanyuan” (CV 4) were punctured first, followed by moxibustion for 3 min, once daily. At the end of each experiment, all the mice were decapitated, and micronucleus incidence of the bone marrow polychromatic erythroblasts (10,000/group) and nucleated cells (10,000/group) were observed and calculated under light microscope. Results:Compared with normal control group, the incidences of micronuclei of polychromatic erythroblasts and nucleated cells of model group Ⅰ and Ⅱ were significantly higher (P<0.001), suggesting that CP could cause the bone marrow cells to generate mutation. In comparison with model group Ⅰ and Ⅱ, the incidences of micronuclei of these two types of cells in prevention group Ⅰ, Ⅱ, Ⅲ and Ⅳ and treatment group Ⅰ were significantly lower (P<0.001). While in comparison with model group Ⅱ, the incidences of micronuclei of these two types of cells in treatment group Ⅱ were also considerably lower (P< 0.001). It showed that acupuncture and moxibustion work well in relieving and preventing CP induced mutation of the bone marrow cells in mice. Conclusion:Acupuncture and moxibustion of “Zusanli"(ST 36) and “Guanyuan"(CV 4) can prevent and relieve intraperitoneal administration of CP induced micronucleus formation of bone marrow cells in the mouse.关键词：微核;针灸;抗突变772|0|0更新时间：2023-08-11
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- 摘要：目的 :观察复合应激致慢性疲劳大鼠模型行为学的变化以及电针“四关”穴的调整作用。方法 :大鼠随机分为四组 ,即正常对照组、模型组、电针四关穴组及电针它穴组。应用慢性束缚加冷水游泳的复合应激因素造模。采用旷场实验、鼠尾悬挂实验和力竭游泳实验观察大鼠行为学的变化 ,并从行为学角度对模型及针刺的防治作用进行评定。结果 :复合应激致慢性疲劳大鼠出现体力下降及抑郁样行为 ,电针“四关”穴可改善这种抑郁样表现 ,并能对抗体力的下降。结论 :电针四关穴能够明显防治应激所致的慢性疲劳 ,改善体力活动 ,对抗抑郁情绪Objective: To observe the effect of electroacupuncture (EA) of “Si guan" points [bilateral “Hegu"(LI 4) and "Taichong" (LR 3)] on the behavior of chronic fatigue rats. Methods: 32 Wistar rats were evenly randomized into control group, model group, EA group 1 (“Si guan" points) and EA group 2 [“Baihui"(GV 20) “Zusanli"(ST 36) “Taichong"(LR 3)]. Chronic fatigue model was established by forcing the rat to swim in cool water (30 min/time) and being trussed up (3 hr/time), continuously for 21 days. EA (4.5 V, 2～20 Hz) was applied to the aforementioned acupoints for 20 min, once daily and continuously for 21 days. The fatigue rats' behavior was tested by employing a specially made open box to record the rat's staying duration in the central check, check bestriding times, embellishing times and duration [bottom leaving (above 1 cm) times and duration of the forelimbs], and by tail object suspending test (motionless duration and struggling times) and exhausted swimming duration. Results: In comparison with control group, the tail suspending motionless duration of model group increased significantly (P<0.05), and the struggling times and the exhausted swimming duration of model group decreased strikingly. After EA treatment, compared with model group, the check bestriding times, struggling times and the exhausted swimming duration of EA group 1 and the exhausted swimming duration of EA group 2 increased considerably (P<0.01); while the tail suspending motionless duration of both EA group 1 and group 2 reduced markedly (P<0.01). Conclusion: EA of bilateral “Hegu" “Taichong" can relieve and prevent chronic fatigue, improve physical performance and resist mental depression, and the effect of “Si guan" is slightly better than that of EA of “Baihui" “Zusanli" “Taichong".关键词：慢性疲劳;电针;四关穴;行为学影响1073|0|5更新时间：2023-08-11
- 摘要：目的 :通过观察去卵巢术和电针对大鼠脑内催乳素释放肽 (prolactinreleasingpeptide,PrRP)的影响 ,以进一步探讨PrRP是否参与生殖内分泌功能调节及其在电针调整雌性生殖内分泌功能异常中的作用。方法 :动物分为假手术组 (Sham)、去卵巢组 (OVX)和去卵巢 +电针组 (OVX+EA)。用放射免疫法 (RIA)测定电针前后去卵巢大鼠血清中催乳素 (prolactin ,PRL)含量的变化 ;免疫组化和RT PCR观察电针及去卵巢对大鼠脑内PrRP蛋白及mRNA表达的影响。结果 :OVX大鼠与Sham组相比 ,血清PRL水平、孤束核PrRP免疫阳性细胞数、终纹床核PrRP阳性纤维相对光密度值及延髓PrRPmRNA都显著降低 (P <0 .0 1或 0 .0 5) ;OVX +EA大鼠与OVX组相比 ,以上指标均显著升高 (P <0 .0 5)。结论 :PrRP可能参与雌性大鼠下丘脑 垂体 卵巢轴 (HPOA)的调节 ;电针对HPOA异常功能的调整作用 ,可能部分是通过脑内PrRP系统实现的Objective: To investigate the effect of electroacupuncture (EA) and ovariectomy on the expression of prolactin releasing peptide (PrRP) in the brain of rats and to probe whether PrRP is involved in the regulation of reproductive endocrine system and to study the underlying mechanism of EA in normalizing the function of reproductive endocrine system. Methods: 24 SD rats were randomly and evenly divided into sham operation group, ovariectomized (OVX) group and OVX+EA group. EA (2～3 Hz, 1～2 mA) was applied to “Guanyuan” (CV 4), “Zhongji” (CV 3), bilateral “Zigong” (EX CA 1) and unilateral “Sanyinjiao” (SP 6) for 30 min, once daily and continuously for 3 days. Serum prolactin contents before and after EA were assayed by radioimmunoassay technique. Expression of PrRP protein and mRNA was analyzed by using immunohistochemical and reverse transcription polymerase chain reaction (RT PCR) methods. Results: Serum prolactin content, the number of PrRP immunoreaction (IR) positive neurons in nucleus tractus solitarii (NTS) of medulla oblongata and the relative optical density value of PrRP positive fibers in bed nucleus of stria terminalis (BST) and expression level of PrRP mRNA in medulla oblongata in OVX group were significantly lower than those of sham operation group (P<0.05～0.01), while those of EA+OVX group were significantly higher than those of OVX group (P<0.05). Conclusion: PrRP may be involved in the regulation of female hypothalamus pituitary ovary axis (HPOA) and EA may normalize the deviant function of HPOA via affecting PrRP expression in medulla oblongata.关键词：电针;去卵巢大鼠;催乳素释放肽975|0|0更新时间：2023-08-11
- 摘要：目的 :通过肿瘤坏死因子 (TNFα)基因表达 ,研究针灸治疗绝经后骨质疏松症的作用机理。方法 :8个月龄SD雌性大鼠 40只 ,双侧卵巢切除后 ,均分为模型、针刺、艾灸、西药四组。相同水、饲料喂养 3个月后 ,针刺、艾灸、西药组治疗 3个疗程。将 40只鼠断头取血 ,检测血清中骨钙素(BGP)、雌二醇 (E2 )含量 ;取双侧股骨测骨密度 ;无菌条件下取出大鼠右侧胫骨 ,分离骨髓细胞 ;利用对L92 9细胞的杀伤作用检测TNFαmRNA生物活性 ;利用RT PCR技术检测TNFαmRNA表达。结果 :针刺、艾灸组BGP、E2 较模型组有显著增高 (P <0 0 5) ;L92 9死亡率较模型组明显减少(P <0 0 0 1) ;RT PCR显示TNFαmRNA表达较模型组明显减少。结论 :针刺、艾灸治疗绝经后骨质疏松症是在基因转录后水平上进行的 ,即针灸治疗绝经后骨质疏松症是通过调控TNFαmRNA表达 ,从而控制TNFα蛋白合成来实现的Objective:To observe the effect of acupuncture on tumor necrosis factor (TNFα)expression in bone marrow of experimental osteoporosis rats for studying mechanisms of acupuncture in the treatment of postmenopausal osteoporosis. Methods:40 ovariectomized SD rats were evenly divided into control, acupuncture, moxibustion and medication groups. In acupuncture group, “Mingmen" (GV 4), “Dazhui"(GV 14), “Dazhu"(BL 11), “Shenshu"(BL 23), “Pishu"(BL 20) and “Zusanli" (ST 36) were punctured and stimulated with uniform reinforcing and reducing method (once every other day, 36 times altogether). In moxibustion group, the abovementioned acupoints were used for moxibustion, 2 moxa cones every acupoint. Animals of medication group were fed with Nilestriol (0.5 mL/100 g, once every week). The contents of serum ositeocalcin(BGP)and estradiol (E 2)contents were measured with radioimmunoassay. Bone mineral density(BMD)of bilateral femora, tumor necrosis factor (TNF)α bioactivity (L929 cell death rates) were measured and TNFα expression of the bone marrow (tibia) tissue cells was measured with RT PCR method.Results: Contents of BGP and E 2 of acupuncture and moxibustion and E 2 of medication groups were obviously higher than those of control group (P<0.05, 0.01). BGP level of medication group was also significantly lower than those of acupuncture and moxibustion groups (P<0.01). No significant differences were found among acupuncture, moxibustion and medication groups in E 2 levels, and between acupuncture and moxibustion groups in BGP levels (P>0.05). The BMD values of the 3 treatment groups were all significantly higher than those of model group (P<0.05); while TNFα bioactivity of the 3 treatment groups were considerably lower than that of control group (P<0.001). No significant differences were found in BMD and L929 death rates among the 3 treatment groups (P>0.05). The situations of TNFα mRNA expression presented a similar tendency to those of L929 death rate. Results suggest that acupuncture and moxibustion induced upregulation of BGP and E 2 and downregulation of TNFα mRNA expression and TNFα bioactivity may contribute to their effect in resisting osteoporosis. Conclusion:Acupuncture and moxibustion can relieve the decrease of E 2 and BGP levels, and downregulate TNFα mRNA expression and the bioactivity of TNFα in ovariectomized rats.关键词：骨质疏松;针刺;艾灸;TNFα1021|0|9更新时间：2023-08-11
- 摘要：目的 :观察电针对大鼠蓝斑 (locusceruleus ,LC)内一氧化氮合酶 (NOS)阳性神经元表达的影响。方法 :采用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶 (NADPH d)法结合计算机图像分析技术 ,观察电针大鼠一侧“足三里”穴对LC内NOS表达的影响。结果 :电针大鼠一侧“足三里”穴后 ,同侧LC内NOS阳性神经元的数量增多 (P <0 .0 5) ,染色加深 ,细胞平均灰度值明显降低(P <0 .0 1) ;非电针侧较正常组无明显改变 (P >0 .0 5)。结论 :电针对大鼠同侧LC内NOS阳性神经元的表达有上调作用Objective: To observe the effect of electroacupuncture (EA) on nitric oxide synthase (NOS) expression of neurons in locus ceruleus (LC) in rats. Methods: 16 SD rats were evenly randomized into control group and EA group. EA (3 V, 2 Hz, wave duration 1 ms) was applied to unilateral “Zusanli"(ST 36) for 30 min. NOS expression was assayed with nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH d) method and measured by using computer image analysis system to detect the optical density value of the stained tissue slices, and morphological changes of NOS positive neurons were observed under light microscope. Results: In animals of control group, most NOS immunoreaction (IR) positive neurons of LC presented moderate density of staining and fewer showed dense and mild staining, while in animals of EA group, NOS IR positive neurons of LC on EA side increased significantly in number in comparison with those of the contralateral side of EA. In addition, on EA side, densely stained neurons in LC increased apparently in number, cellular average optical density value lowered strikingly (P<0.01). No significant differences were found between EA group (non EA side) and control group in IR positive neuron number and the average optical density value (P>0.05). Conclusion: Electroacupuncture of “Zusanli"(ST 36) can upregulate NOS expression of neurons in the ipsilateral LC.关键词：一氧化氮合酶;蓝斑;电针967|0|1更新时间：2023-08-11
- 摘要：目的 :观察头颅CT定位围针治疗多发性梗塞性痴呆的疗效 ,及对患者生存质量的影响。方法 :选择多发性梗塞性痴呆患者 50例 ,随机分为电围针 (头颅CT定位 )组 2 5例 ,西药 (尼莫通 )组 2 5例 ,对患者治疗前后的临床疗效和生活质量进行评定。结果 :相同疗程后 ,两组的各观察指标均有显著改变 ,且治疗后电围针组生存质量的评分明显提高。结论 :头颅CT定位围针对多发性梗塞性痴呆患者的生存质量有一定的改善作用Objective: To observe the effect of CT aided scalp surrounding needling on the life quality of multiple cerebral infarction dementia (MCID) patients. Methods: 50 MCID patients were randomly and evenly divided into scalp surrounding needling (treatment) group and medication (control) group. In treatment group, the scalp points (used as main points) around the cerebral infarction focus projection region shown by CT scanning and body acupoints as Fenglong (ST 40), Ganshu (BL 18), etc. were punctured and the scalp points were stimulated with an electroacupuncture stimulator once daily, continuously for 9 weeks. Patients of control group were ordered to take Nimodipine (30 mg/time, t.i.d., 9 weeks). The therapeutic effect was evaluated by using “Criteria for Diagnosis, Syndrome differentiation and Curative effect evaluation of Senile Dementia" and the living quality assessed using Karnofsky performance status(KPS). Results: After treatment, of each 25 cases of treatment and control groups, 5 and 2 had remarkable improvement, 17 and 12 experienced improvement, 3 and 11 had no apparent changes, with the effective rates being 88.0% and 60.0% respectively. The therapeutic effect of treatment group was significantly better than that of medication group (P<0.05). Self comparison of each group in KPS values showed that only KPS of treatment group (45.20± 4.48) increased significantly in comparison with pre treatment (31.84±4.32), and was strikingly higher than that (31.60±4.85) of medication group (P<0.05). Conclusion: CT aided scalp surrounding needling has a positive effect in improving the life quality of multiple cerebral infarction dementia patients.关键词：头颅CT定位围针;多发性梗塞性痴呆;生存质量828|0|1更新时间：2023-08-11
- 摘要：目的 :探讨针刺大赫、水道治疗膀胱无力型女性尿道综合征 (FUS)的疗效。方法 :观察针刺前后 ,膀胱无力型FUS患者尿动力学指标的变化。结果 :针刺治疗的总有效率为 91.67% ,与治疗前比较患者的尿动力学各项指标均有明显改善 (P <0 .0 5～ 0 .0 1)。结论 :针刺大赫、水道可以提高膀胱逼尿肌的压力 ,改善尿流率 ,从而缓解因膀胱无力而引起的排尿功能障碍Objective: To study the therapeutic effect of acupuncture of Dahe (KI 12) and Shuidao (ST 28) on female urethral syndrome of bladder inertia type. Methods: A total of 120 cases of urethral syndrome of lower intravesical pressure women were observed in the present paper. Dahe (KI 12) and Shuidao (ST 28) were punctured with gauge 30 stainless steel needles (3 cun long) and stimulated electrically with an electroacupuncture therapeutic apparatus for 20 min after gaining needling sensations. The treatment was conducted once daily, with 10 times being a therapeutic course. Clinical symptoms of urinary frequency, urinary times during night, urinary dysfunction, lower abdominal distension, etc. and urinary dynamics (detrusor urinae pressure, maximum uroflow rate and intraabdominal pressure) were detected. Results: Following one course of treatment, of the 120 patients, 101 (84.17%) experienced marked improvement, 9 (7.50%) had improvement in their symptoms and 10 (8.33%) failed in the treatment, with the effective rate being 91.67%. In comparison with pre treatment, values of detrusor urinae pressure, maximum uroflow rate and intra abdominal pressure increased significantly (P<0.05～0.01). Conclusion: Acupuncture can improve clinical symptoms and urodynamics of patients with urethral syndrome of acraturesis type.关键词：膀胱无力;尿道综合征;针刺;尿动力学967|0|1更新时间：2023-08-11
- 摘要：络系统是经络系统的一个有机组成部分。络系统的组成主要有十五大络、阴络、阳络、缠络、孙络和浮络等。它是运行人体气血、沟通脏腑肢节、调整人体生理功能的基本功能单位。络病理论认为络病是由各种因素而导致络脉痹阻、气血津液运行不畅的一类病变。深入研究络系统和络病理论对于我们继承和发展中医传统理论 ,并对其中的脏腑经络、病因病机、诊断治疗、辨证论治等理论都有很大的帮助。并且对于运用现代医学对传统中医进行微观研究 ,提出相应的假说有一定的意义。关键词：传统中医;络系统;络病理论840|0|0更新时间：2023-08-11
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