最新刊期
卷 ， 期 2 ， 1986
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- 摘要：56 rats were divided into 4 groups: (a) controls, (b) elerctoacupu- ncture-(EAP-) treated, (c) morphine-treated and (d) morphine plus EAP treated. Besides using the PAP technique of Sternberger for immunocyto- chemical demonstration of SP and ENK and the KarnovskyRoots' method for the histochemical visualization of ACHE, additional sections stained for SP and AChE were prepared for electron microscopic (EM) observati- ons. A semi-quantitative analysis of tissue sections was also accomplished. After EAP the elevation of pain threshold was correlated with the enhanced staining of SP-LI in Rexed's lamina Ⅱ (P<0.001 as compared with the control group). However, ENK-LI in the same regions appeared decreased (P<0.001) . After morphine, SP-LI in lamina Ⅱ became increased (P<0.001) rese- mbling that after EAP. However, when the morphine-treated animals were also subjected to EAP, SP-LI became diminished instead (P<0.001, compared with the morphine group) . As for the histochemical changes of AChE in dorsal horn, the levels of enzymic activity of animals in various groups were different, e.g. 3.855 ±0.550 (in control group), 4.225±0.477 (EAP), 3.252±0.405 (morphine) and 3.775±0.401 (morphine plus EAP group). Indeed Rexed's lamina Ⅱ is rich in both SP-LI and AChE. EM obser- vations showed that SP-LI material appears in and around clear synaptic vesicles in the axonal ending of "thin" diameter, whereas AChE activity distributes mostly within axon of "coarse" caliber. The foregoing results indicate that the effects of EAP and morphine analgesia are similar with regard to the change of SP-LI, but opposite with respect to the change of AChE activity. It is suggested that the separate effect of EAP and morphine on SP-LI and ENK-LI can be expla- ined by Jessell-Iversen model of spinal processing. And a possible func- tional relationship between SP and acetylcholine can not be excluded. The AChE-marked neurons may influence SP release.1016|0|0更新时间：2023-08-11
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- 摘要：It has been reported that the recovary of acute ischemic myocardial injury and ischemic ECG could be promoted by electro-acupuncture(EA) at acupoint "Naiguan", these suggested that EA was favorable to the impro- vement of cardial electric stability in the early stage of ischemia. The present investigation was carried out to study the effect of EA on the ischemic myocardial electric activity, the monophasic action potential (MAP) was used as the index. 30 mongrel rabbits of either sex, weighing 2.3-2.8 kg. were rendomly divided into ischemic, electro-acupuncture and control group. All the ani- mals anesthetized with sodium pentobarbital and incubated with an endo- trachacheal tube. Following artificial positive-pressure ventilation and immobilized with flaxedil, a left thoractomy was performed through the surface of the 4th costa. The heart was exposed and suspend in a pericar- dial cradle. The model of myocardial ischemia in this study was produced by twice ligation of the ventricular branch of the left coronary artery(LVB).LVB was occluded for 8 min first and reperfused for 50 min, then occluded again for 60 min The animals of control group didn't undergo the liga- tion of LVB. The procedure of EA group was the same as the above except perfor- ming EA at "Neiguan" from 1 min after the first ligation to the immediate time after release, and from 1 min to 10 min after the second ligation. MAP was recorded by epicardial contact electrode(ECE).The recording electrode was placed perpendicularly, with the pressure of 150-200g, on the apex of the heart during the first ligation and raperfusion, and in the regions of center and border zone respectively for the second ligation; the referential electrode was placed in the hypoderm of neck. In this study, MAP and standard lead Ⅱ of ECG at different period prior to and after the first ligation and reperfusion, and MAPs at the different period prior to and after the second ligation of LVB were recor- ded. MAP Amplitude(MAPA), MAP Duration of 50% and 90% repolariza- tion(MAPD_(50) and MAPD_(90))were used to evaluate the electric activity condition of myoeardium, ST segment of ECG lead Ⅱ(ST_(Ⅱ))was used to reflex the myocardial isohemic condition in the early stage of LVB occlu- sion. The heart was excised immediately after the experiment was finished, and superperfused with 37℃ normal saline via aorta. The maximal transve- rse and longitudinal diameters of ischemic region were measured roughly. After the first ligation of LVB, the MAPA in the ischemic group was reduced rapidly and decreased by about 50% at 8 min, comparing it with its own value before the ligation(P<0.01).Meanwhile, MAPD_(50) and MAPD_(90) shortened obviously(P<0.01). In EA group, MAPA was decreased only by 27% at 8 min, slight cha- nges of MAPD_(50) were also observed; MAPD_(90) in this group remained essentially unchanged during the occlusion of LVB, comparing three indices with those of ischemic group, the differences were significant(P<0.01). Following the reperfusion in the earlystage of myocardial ischemia, MAPA in ischemic group went up to the maximum in 1-3 min, then, fell down continuously, accompaning with the further shortening of MAPD_(90); on the contrary, the MAPD_(50) in this group had the tendency to recover, although it was still shorter than the pre-ligation value at 50 min(P<0.05). These couldn't be considered as the recovery of the membrane mechanism. However, in EA group, MAPA was almost unchanged after reaching the maximum and similar to that of the control group(P>0.05).MAPD_(50) was also recovered immediately after reperfusion, and returned to the pre-liga- tion value(P>0.05)within 5 min. Moreover, MAPD_(90) was always remai- ned at the level of pre-ligation value(P>0.05)during the whole period of reperfusion. It was suggested that EA could normalize the ischemic myo- cardium which was damaged reversibly. The early changes of MAP both in border zone and center zone of isc- hemic group after the second ljgation of LVB were similar to those after the first ligation, but the degree of MAP alterations in border zone was smaller. As time of occlusion went on, MAPA in center zone declined con- tineously while the changes of MAPD_(50) among the individuals of the isc- hemic group tended to dispersion, these were different from those in bor- der zone which the decrease of MAPA didn't company with the disper- sion of MAPD_(50). The changes of MAP in center zone of EA group were almost same as those of ischemie group, no significant differences were found between two groups. This indicated that EA could hardly act on the ischemic center zo- ne. However, the the declination of MAPA in border zone was slowed do- wn obviously by the EA and MAPD_(50) returned to normal gradually, too. In addition, concomitant with the normalization of electrophysiological condition of border zone, the maximal transverse diameters of ischemic region in all animals of EA group were also reduced remarkedly(P<0.01). All results mentioned above implicated that EA could save the jeopardiz- ed myocardium, reduce the size of infarction, correct the disorder of cardial electric activity and increase the electric stability, these effects will help to reduce the mortality of coronary heart disease resulted from se- rious arrhythmia.1004|0|0更新时间：2023-08-11
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- 摘要：For the purpose of studying the mechanism of the propagated sensation along channels (PSC) and clarifying the relationship between PSC and the central neuvous system, this paper was designed to observe the chara- cteristics of cortical somatosensory evoked potentials (SEP) in the subjects with marked PSC. The investigation was performed on 10 subjects, whose PSC was kept stable, distinct and could reach the head, and 16 subjects without PSC. Electrical stimulation was delivered to the Jiaxi or Hegu. SEP were recorded simultaneously from four scalp points: corresponding to the areas of foot, arm representation contralateral to the stimulated side and bilateral face representation of somatosory area I(SI). The results are shown as follows: 1. In addition to the PSC perceived by the subject, a complex poten- tial consisting of 5 elements (P_1, N_1, P_2, N_2, P_3) could be recorded from the point corresponding to the contralateral foot representation in stimu- lating Jiaxi in the subjects with marked PSC. Their mean peak latencies were 45.1±4.63, 57.1±5.71, 72.9±7.34, 84.8±11.1 and 106±10.4 msec, respectively. The mean amplitudes of components C_1, C_2, C_3, C_4, C_5, which were composed of N_0-P_1, P_1-N_1, N_1-P_2, P_2-N_2, N_2-P_2, were 0.47± 0.19, 0.87±0.47, 0.98±0.52, 1.68±1.24, 1.41±0.16μv, respectively. View- ing on the distribution characteristics of SEP, the amplitude was the lar- gest in contralateral foot representation area but decreased markedly in other areas. The similar distribution was observed in 16 subjects without PSC. 2. Comparing the SEP from the foot representation area of the sub- jects with marked PSC with that of the subjects without PSC, the ampli- tudes of the majority of the components in the former were smaller than those in the latter, but there was no significant difference (P>0.05) bet- ween the two groups except for components C_4-C_5. The amplitude of com- ponents C_4-C_5 in the subjects with marked PSC was 2.6±1.59μv, while in the subjects without PSC was 4.24±1.83, the difference was signifi- cant (P<0.05). In accordance with the reports that the components C_4- C-5 might have a close relation to the perceptive procese of the somatic stimulation, we also found that there was a positive correlation between componets C_4-C_5 and the intensity of the needling sensation. 3. The results similar to those mentioned above were also observed when hegu was stimulated. 4. Observation on imitation of PSC was carried out in 10 subjects without PSC. The method of imitation was as follows: a soft painting bru- sh was moved repeatedly along the Gall Blabder Channel from foot to fa- ce at a speed of 20 cm per sec. The mean amplitude of components C_4-C_5 was 2.84±0.57μv during imitation. The control values derived from the recording before and after imitation were 4.45±0.7 and 4.85±1.02μv, re- spectively. The amplitude of component C_4-C_5 was markedly decreased during imitation (P<0.01) but recovered completely after imitation. The results demonstrated that the amplitude of components C_4-C_5 in the subje- cts with marked PSC was nearly the same as that in the subjects without PSC during imitation (P>0.5). The decrement of the amplitude during imitation was just equal to the difference between the two groups. The results mentioned above revealed that SEP in the subjects with marked PSC differed from that in the subjects without PSC. Components C_4 -C_5 of the former were much smaller than those of the latter. It seem that during the advance of PSC the functional activities of SI of the cere- bral cortex were interfered by some factors, which led to the decrease of the amplitude of SEP. This is a sensory physiological evidence of the existence of the PSC in reality. However, the cause of the interference remains to be further carified. Based on the results of imitation of PSC and the fact that the irradiation of excitation is limited by the surround inhibition and other mechanisms in somatosensory system, of which this is an important characteristic feature, it could be expected that the inter- ference of the functional activities of SI may be a reflection of the ac- tivation of the peripheral nerve fibers and receptors insequence by a certain agent advancing along the channels.1037|0|0更新时间：2023-08-11
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