摘要:目的 :观察电针对实验性血管性疾呆 (VD)大鼠学习记忆能力和脑组织细胞凋亡的影响。方法 :采用 4 血管阻断模型 ,用Morris水迷宫测定大鼠学习记忆能力 ,并用Tunel法检测鼠脑皮质和海马细胞凋亡情况。结果 :模型大鼠表现明显的学习记忆障碍 ,在定位航行试验中 ,与假手术组相比 ,潜伏期显著延长 ;在空间探索试验中 ,在原平台象限跨越平台次数与其余三个象限无显著差异 ;其顶叶皮层及海马有大量的凋亡细胞出现。而电针能显著缩短定位航行试验的潜伏期 ;在空间探索试验中 ,在原平台象限跨越平台次数显著多于其余三个象限 ,与假手术组无显著差异 ;其顶叶皮层及海马CA1区的凋亡细胞数显著减少 ,受损程度明显轻于模型组。结论 :电针能改善VD大鼠学习记忆能力 ,有拮抗脑组织细胞凋亡的作用Objective: To observe the effect of electroacupuncture (EA) on learning and memory and apoptosis of hippocampal neurons in experi ment al Vascular Dementia (VD) rats. Methods: A total of 50 SD rats were r andomly divided into sham-operation group ( n =10), model group ( n =13), E A group ( n =14) and medication group ( n =13). VD model was established by using 4-vessel-occlusion method. EA (2 mA, 150 Hz) was delivered to "Baihui " (GV 20) and "Dazhui" (GV 14) for 20 min through the inserted acupuncture need les, once daily and continuously for 15 days. In medication group, Nimodipine (1 2 mg/kg) was fed to the rats once daily, 15 days altogether. Learning and memory was determined by water Morris method. Apoptosis of the brain tissues was detected by Tunel method. Results: Place navig ation test showed that compared with sham-operation group, the escape latency of rats in m o del group increased considerably ( P <0.01); while in comparison with model g roup, the increased escape latency values of rats in EA and medication groups were sho rtened significantly ( P <0.01). No significant differences were found between EA and sham-operation, and between medication and sham-operation groups ( P >0. 05). Sp atial probe test displayed that the number of rats passing through the original platform in model group was obviously smaller than that of rats in the rest 3 groups ( P <0.01), while no significant differences between EA group or medic atio n group and sham-operation group ( P >0.05). It showed that administration of EA a nd Nimodipine could improve the learning and memory of VD rats. The apop totic neurons of EA and medication groups were significantly fewer than those of model group in both the cerebral cortex and CA1 area of hippocampus, and no sig nificant difference was found between EA and medication groups, suggesting that both EA and medication could suppress ischemia-reperfusion induced apoptosis. Conclusion: Electro-acupuncture can improve the learning and memory of VD rats and inhibit cerebral apoptosis after cerebral ischemia-reper fusion.
摘要:目的 :观察电针对实验性血管性痴呆 (VD)大鼠海马神经胶质细胞和毛细血管的影响。方法 :实验用 4 血管阻断模型 ,用Morris水迷宫测定大鼠学习记忆能力 ,并用电镜观察鼠脑海马神经胶质细胞和毛细血管的超微结构变化。结果 :模型组海马神经组织超微结构的形态损害明显 ,胶质细胞变性、水肿 ,毛细血管内皮细胞受损。电针能明显减轻或抑制缺血的大鼠海马神经胶质细胞及毛细血管的损害。结论 :电针明显改善痴呆大鼠的学习记忆能力 ,与其对脑组织神经元、神经胶质细胞及微血管的全脑保护作用密切相关Objective: To observe the effect of electroacupuncture (EA) on neuroglial cells an d capillaries of hippocampus in vascular dementia (VD) rats. Methods: Rat VD mod el was established by using four-vessel occlusion (4-VO) method, and the rats were randomized into model group, EA group and sham-operation group, with 5 rat s in each group. EA (150 pulses/min, 2.0 mA, 20 min) was delivered to "Baihui"( GV 20) and "Dazhui"(GV 14) once daily, continuously for 10 days. The brain tiss ue was cut into sections for observing ultrastructural changes of neuroglial cel ls and capillaries in the hippocampus under transmission electron microscope. Results: In model group, the cellular membrane and the nuclear membrane of neuroglial c ells in hippocampus were not clear, the chromatin condensed, the perinuclear org anelles decreased in number, disintegrated or disappeared, presenting a big blan k region. The hippocampal capillaries showed deformed lumen, indistinct basement membrane, swelling of the endothelial cells, and decrease of nerve synapses. Wh ile in acupuncture group, the ultrastructural changes of neuroglial cells were m uch lighter, such as light swelling of the cellular nuclei and membrane and cyto lipin with condensation of the nuclear chromatin but without blank zone; and the mitochondria were close to normal. The capillaries showed distinct and smooth l umen, basically intact basement membrane with slight swelling at the periphery p art of the membrane, being apparently lighter than those of model group in the s tructural injury. Conclusion: EA could markedly suppress ultrastructural lesion of neuroglial cel ls and capillaries in hippocampus of VD rats, and lessen the damage of cerebral tissue.
摘要:目的 :观察电针对全脑缺血再灌注损伤后高龄大鼠大脑皮质P5 3蛋白表达的影响。方法 :采用改良的Pulsinelli 4 血管阻断 ( 4 VO)方法制备SD高龄大鼠全脑缺血再灌注损伤模型 ,将大鼠随机分为假手术组、缺血再灌注组、缺血再灌注 +电针组。假手术组动物仅烧灼双侧翼小孔内椎动脉 ,但不夹闭双侧颈总动脉 ,电针组在缺血再灌注后立即予电针治疗 ,以后每天 1次。在缺血再灌注 3天 ( 72hr)后将大鼠处死 ,进行免疫组织化学染色。结果 :缺血再灌注组大鼠大脑皮质P5 3蛋白免疫阳性细胞数明显高于假手术组 (P <0 <0 5 )。缺血再灌注 +电针组大鼠大脑皮质P5 3蛋白免疫阳性细胞数明显低于缺血再灌注组 (P <0 <0 5 )。结论 :电针可明显减少全脑缺血再灌注损伤后高龄大鼠大脑皮质P5 3蛋白的表达 ,这可能是电针抗脑缺血后神经元凋亡的途径之一Objective:To observe the effect of electroacupuncture( EA) on P53 p rotein expression in rats with cerebral ischemia-reperfusion injury and to anal yze the mechanisms of EA in reducing neuronal apoptosis. Method: 36 S D rats were randomly and evenly divided into sham-operation group,model group an d EA group.Cerebral ischemia-reperfusion model was established by using modifie d (Pulsinelli's)4-vessel occlusion method. Bilateral"Zusanli"(ST 36) were pu nctured and stimulated with an EA therapeutic apparatus (100 Hz ,3.5 mA and dura tion of 60 min),once a day,3 days altogether. Immunohistochemical method was use d to display P53 protein expression in the cerebral cortex of elder rats with ce rebral ischemia-reperfusion injury. Results: Compared with sham-ope r ation group,the number of P53 immuno-reaction positive cells markedly increased in ischemia-reperfusion group ( P <0.05). In EA group, the number of P53 imm uno-reaction positive cells was significantly fewer than that of model group ( P <0.0 5),suggesting that EA could down-regulate P53 protein expression in rats with c erebral ischemia-reperfusion. Conclusion:EA could down-regulate P53 protein expression in cerebral ischemia rats, that may contribute to the actio n of EA in reducing cerebral neuronal apoptosis.
摘要:目的 :采用mRNA AFLP技术 ,探讨SAMP10衰老相关性脑细胞基因表达的变化及针刺的干预作用。方法 :应用由磁珠分离法纯化mRNA ,SMART PCRcDNA合成和AFLP 银染法结合形成的mRNA AFLP分析技术 ,进行mRNA指纹检测 ,寻找衰老相关性差异表达的基因片段 ,并观察针刺对其表达水平的影响 ,研究衰老相关基因与针刺的关系。结果 :发现规律性变化的一条cDNA带与多品系小鼠的线粒体DNA、编码小鼠ATPase亚基 6和大鼠ATPase亚基 6和亚基 8的mRNA及编码大鼠线粒体细胞色素C氧化酶亚基Ⅰ、Ⅱ、Ⅲ的部分基因序列均有高度同源性。实验表明该cD NA带呈现衰老相关性低表达 ,并可受针刺单穴“水沟”、“内关”和“后三里”的良性干预。结论 :SAMP10的快速衰老与体内能量代谢障碍有关 ,针刺可能从分子水平影响了能量代谢的相关因素 ,这可能是针刺干预衰老进程的机制之一Objective: By using mRNA-amplified fragment length po l ymorphism (AFLP) technique, senescence-associated changes of gene expression of the cerebral neurons and the effect of acupuncture intervention were observed i n senescence-accelerated mouse (SAM)P10 and SAM R1. Methods: Six ty SAM P10 were randomly divided into "Shuigou"(GV 26), "Neiguan"(PC 6), "Tai chong"(LR 3), "Shenshu"(BL 23), "Zusanli"(ST 36) and P10-control groups, in a ddition, 10 SAM R1 were made up of R1-control group. These 5 acupoints were st imulated with handle acupuncture, once daily, continuously for 2 weeks, with one day's rest between two weeks. Magnesium pearl separation-purification method w as used to isolate and purify mRNA from the total RNA of the whole brain. SMART PCR-amplified cDNA synthesis and AFLP-silver staining method combined with mRNA-AFLP analysis technique were applied in the detection of mRNA fingerprint to search for the differentially expressed gene fragments associate d with the senescence and to study the effect of acupuncture intervention on the gene level of these fragments. Results: In comparison with the differentially-expressed gene ban d of the normally-aged mice of R1 control group, the coloration of the band of rapidly-aged mice of P10 control group was lightened (OD value was smaller). Af ter acupuncture of GV-26, PC-6 and ST-36, the OD values of the differentially -expressed gene band increased, while no obvious changes were found in acupunct ure of LR-3 and BL-23 groups. It shows that in SAMP 10, the expression of cDN A fragments being of high autoploidy with mitochondrial DNA and mRNA of the enco ded ATPase subunit and cytochrome C oxidase subunit was lower; while acupuncture stimulation of GV-26, PC-6 and ST-36 can suppress this abnormal change of se nescence-associated low expression of gene fragment of cDNA, suggesting an anti -aging effect of acupuncture. In addition, one regular change of cDNA band in S AM R1 and P10 was found to have a high autoploidy (86%~97%) with some known seq uences of the GenBank, like mitochondrial DNA of multiple species of mice, ATPas e subunit 6mRNA of mice and ATPase subunit 6mRNA and 8mRNA of rats, and partial gene sequence of mitochondrial cytochrome C oxidase subunit Ⅰ, Ⅱ and Ⅲ of rat tus norvegicus. Conclusion: The disturbance of energy metabolism plays a role in the acceleration of senescence of SAM P10 and acupuncture may exert its favorab l e influence on themolecular factor-related energy metabolism, which might be on e of the mechanisms of acupuncture in anti-aging effect.
摘要:目的 :观察前后交替针刺加手法治疗中风急性期后下肢偏瘫的效果。方法 :临床将中风急性期后偏瘫下肢功能障碍患者 5 8例随机分为针刺治疗组和对照组各 2 9例 ,针刺组采用交替针刺下肢之梁丘、足三里、丰隆、委中、承山、太溪加手法的方法治疗 ,对照组施以一般内科治疗。结果 :两组治疗前后Barthel指数及Fugl Meyer下肢运动功能评分均有差异 (P <0 0 1) ,同时 ,治疗后针刺组Barthel指数及Fugl Meyer下肢运动功能评分明显高于对照组 (P <0 0 1)。结论 :前后交替针刺加手法是治疗中风急性期后偏瘫下肢功能障碍的有效方法Objective: To observe the effects of acupuncture and massage i n treating low-limb dysfunction of hemiplegia patients at the sub-acute stage. Methods: 58 Cases of apoplectic hemiplegia patients were randomly and e venly divided into treatment group and control group. Patients of treatment grou p were treated with acupuncture of alternate acupoint group 1) Liangqiu(ST 34), Zusanli (ST 36) and Fenglong (ST 40); and 2)Wizhong (BL 40), Chengshan (BL 57) a nd Taixi (KI 3). In treatment group, the acupoints of group 1 were stimulated wi th uniform reinforcing-reducing needling method, combined with mild massage of t he lower limbs along the running course of the Large Intestine Meridian of Foot - Yangming, and those of group 2 were stimulated with the same manipulation combin ed with mild massage along the course of the Kidney Meridian of Foot-Shaoyin. T h e treatment was conducted once daily, 5 times every week, and with 10 times bein g a therapeutic course. In addition, routine pharmacotherapy including anticoag ulants, hemostats, medicines for improving microcirculation, and for nourishing nerves, etc. was also used. Patients of control group only accepted routine phar macotherapy. Results: Following two courses of treatment, the scor es of Barthel Index and Fugl-Meyer (for assessing the motor functions of the li mbs) were given. The scores of Barthel Index of pre-treatment vs post-treatmen t in control group were 23.3±11.7 vs 37.5±18.6 and those of treatment group w ere 25.1±12.3 vs 53.4±16.7 respectively; the scores of Fugl-Meyer motor func tion of pre- vs post-treatment of control group were 5.67±2.34 vs 11.48±6.92 , and those of treatment group were 5.59±3.26 vs 20.41±8.29 separately. Self -comparison between pre- and post-treatment in each group, the scores of both Bart hel Index and Fugl-Meyer were raised evidently following treatment ( P <0.0 1) . After treatment, the scores of these two indexes of treatment group were all strikingly higher than those of control group ( P <0.01), suggesting that the therapeutic effect of treatment group was significantly superior to that of medi cation. Conclusion: Acupuncture combined with massage is effective in bettering the movement function of the low extremities of stroke patients.
摘要:目的 :观察针刺和黛力新 (Deanxit)治疗抑郁性神经症的疗效及其对免疫功能的影响。方法 :以CCMD 2 R为标准选取 41例抑郁性神经症患者 ,随机分为针刺组 2 1例及西药组 2 0例 ,针刺组以针刺治疗两个疗程共 2 0次 ,西药组服用黛力新治疗 2 0日 ,治疗前及疗程结束后以汉密顿抑郁量表HAMD评分 ,并检验疗程前后IL 2、IL 8值加以比较。结果 :每组组内治疗前后HAMD评分比较均有显著性差异 (P <0 0 1) ,两组治疗后评分比较无显著性差异 (P >0 0 5 ) ;每组组内IL 2、IL 8值治疗前后比较均有显著性差异 (P <0 0 1,P <0 0 5 ) ,同项目治疗后比较均无显著性差异 (P >0 0 5 )。结论 :针刺和黛力新均能有效治疗抑郁性神经症 ,其治疗原理可能与改善患者的免疫功能有关Objective: To observe the therapeutic effect of acupuncture in the treatment of depressive psychosis and to analyze its influence on the immunological function. Methods: A total of 41 depression patients were randomized in to acupuncture group ( n =21) and medication group ( n =20). In acupuncture group, Taiyang (EX-HN 5), Yintang (EX-HN 3), Baihui (GV 20), Neiguan (PC 6), Z usanli (ST 36), etc. were punctured and stimulated electrically with an electroa cupuncture (EA) therapeutic apparatus for 20 min. In medication group, patients were asked to take Deanxit ta blets. Results: After 20 days' treatment, the scores of Hamilton rating scale for depression (HAMD) of two groups lowered significantl y compared with their own pre-treatment ( P <0.01); an d comparison between two groups showed that there was no significant difference between them in HAMD scores ( P >0.05). Following treatment, blood IL-2 and IL-8 contents decreased considerably ( P <0.05~ 0.01) , which had not sign ificant differences between two groups( P >0.05). Conclusion: Both acupuncture therapy and medication work well in the treatment of depressive psychosis and their ther apeutic effects may be closely related to the decline of blood IL-2 and IL-8 l evels.
摘要:目的 :客观评估岭南挑筋法治疗强直性脊柱炎 (AS)的临床疗效和安全性。方法 :将符合AS纳入标准的 81例患者用分层随机方法分为挑筋组 41例 ,对照组 40例 ,以强直性脊柱炎临床常见的 14种症状和体征的分级量化分值作为评价指标进行临床观察。结果 :挑筋组的总有效率为85 4% ,对照组为 5 5 0 % ,两组比较有非常显著性差异 (P <0 0 5 )。挑筋组治疗后除个别患者针挑口偶有瘙痒外 ,余无任何不良反应。结论 :岭南挑筋法能消除或减轻腰背僵痛等临床症状 ,改善脊柱功能活动 ,且安全无副作用 ,是治疗AS较理想的方法Objective: To evaluate the clinical therapeutic effect and saf et y of Lingnan (referring to Guangdong and Guangxi Provinces) Muscle-pricking The rapy in the treatment of ankylosing spondylitis (AS). Methods: 81 AS patients were divided into treatment group ( n =41) and control group ( n = 40) with stratified random method. In treatment group, Jiaji (EX-B 2), back- sh u points of the Bladder Meridian and other acupoints of the Governor Meridian as well as Ashi-points at the neck and the thoracic back and in the lumbar region were used. Following routine sterilization and local anesthesia by subcutaneous injection of 2% procaine, used Gauge-1 needle to break the superficial skin an d pull out the subcutaneous white fibrous tissue slowly, once daily, 4 acupoints every time, with 15 days being a therapeutic course. In control group, AS patie nts were asked to take salicylazosulfapyridine. Results: Following one month's treatment, in treatment and control groups, one and 0 case were cured, 6 and 2 cases had remarkable improvement, 28 and 20 were effective, 6 and 18 failed, with the total effective rates being 85. 4% and 55.0% respectively. Conclusion: Lingnan Muscle-pricking t reatment can effec tively relieve lumbago, stiffness, etc. of AS patients and has no side-effects.
摘要:本文通过系统分析揭示 :足厥阴脉“是动”病描述的是阴疝的症状 ,古人从阴疝发病部位特点及对该病的针灸诊疗经验中抽提出“足背 前阴 少腹 腰 (舌 )之间相关联系”的规律 ,而所谓“足厥阴脉”则是古人对这一规律的直觉解释。这一新发现为考察经络学说的形成过程提供了关键证据 ,引导人们正确分析经络学说的构成要素并进而正确理解该学说的意义与价值In the present paper, the author systematically investigates t he formation proce ss of "Foot-Jueyin Meridian" from the ancient literature and puts forward his viewpoint on the scientific value of meridian-collateral theory, that is, the t r ue connotations of this theory lie in the meridian-bound empirical evidence and the deduced basic regularities of mutual connections among different parts of th e body surface and interrelation between the body surface and the corresponding internal organs, rather than those simple twelve meridian lines. Abundant data show that "Shidong Bing" (是动病meridian disease) of the Foot-Jueyin Merid i an is referred to symptoms and signs of scrotal hernia. In accordance with the c haracters of the affected location of scrotal hernia and the related experience of diagnosis and treatment in acu-moxibustion clinic, the Chinese ancient medic a l experts summed up a set of connection regularities of Foot-Jueyin Meridian t h at starts from the dorsum of foot and goes up to the antero-pudendal region, th e lower abdomen, and to the waist region (or tongue),etc.. The so-called "Foot -Jue yin Meridian" is the ancient's intuitive interpretation about its connection r egularities. This new discovery may provide a key evidence for investigating the development process of the meridian doctrine of TCM and will guide the research ers to analyze the major factors composing the meridian doctrine and to understa nd its actual significance and value correctly.
摘要:为了解针刺调节脑缺血再灌注后细胞凋亡基因表达的研究进展情况 ,本文对近几年的有关文献进行了归纳总结 ,发现针刺对脑缺血再灌注损伤后多种细胞凋亡基因的表达均有调节作用 ,总的说来 ,针刺可抑制促细胞凋亡基因的表达 ,而促进抑细胞凋亡基因的表达。本文还对其机制进行了探讨In the present paper, the authors review recent advan ce s of acupuncture modulation on the expression of apoptosis-related genes in the brain tissues following local cerebral ischemia-reperfusion. Experimental find ings show that: electroacupuncture can 1) up-regulate expression of heat shock protein (HSP, functioning in promoting synthesis of new ribosome and restoratio n of cellular nucleus structure, protecting mitochondria and suppressing apoptos is) and potentiate the transcription of HSP 70 mRNA in the ischemic cerebral tis su es in the rat; 2) suppress or up-regulate expression of c-fos (immediate early gene) protein and c-fos mRNA of hippocampus, cerebral frontal lobe, etc.; 3) u p -regulate expression of Bcl-2 gene (serving to suppress apopotosis) and nerve growth factor (NGF) mRNA and down-regulate expression of Bax (a gene for ant agon izing Bcl-2) and P53 gene (accelerating apoptosis) of the ischemic area; 4) red uce IL-1 mRNA expression of the striate body, and suppress ischemia-induced ri se of blood TNF-α level; and 5) lower cerebral contents of excitatory amino ac ids, inhibit formation of free radicals, and reduce overload of intracellular Ca 2+ . The effects of acupuncture mentioned above may contribute to its bene ficial action in the treatment of cerebral inf arction patients in clinic.
摘要:为了探讨艾灸治疗RA抗炎免疫作用的机理 ,我们建立免疫性炎症———大鼠佐剂性关节炎模型等 ,从神经 内分泌 免疫调控入手 ,在整体、器官、细胞水平开展灸治RA的实验研究。结果艾灸“肾俞”、“足三里”穴具有抗炎免疫作用 ,其机理与灸疗抑制TNF、IL 1等炎症因子的释放 ,增强与改善机体的免疫功能 ,保护胸腺、脾脏等免疫器官 ,纠正炎症时自由基代谢的紊乱 ,调整NO、NE、5 HT神经递质的失衡 ,促进内环境稳定等多方面因素有关。实验证实 ,外周交感神经参与艾灸对免疫的调节 ;灸疗的部分作用是通过肾上腺皮质系统发挥的 ;海马可能是灸疗信息中枢整合的重要环节 ;HPA轴是灸疗作用中一条重要的神经体液性途径 ;松果体 (MT)可能是艾灸抗炎免疫的一个高位调节点。提示灸疗抗炎免疫作用是其治疗RA的主要机制之一In the present paper, the authors summarize their resu l ts of study on the mechanism of moxibustion of "Shenshu"(BL 23) and "Zusanli"( ST 36) for resisting inflammation and modulating immune function in the treatment of rheumatoid arthritis (AA) rats.1)Anti-inflammatory effect: In AA rats, afte r 3 sessions of moxibustion, the c ircumferences of the swollen ankle of BL-23 group (2.33±0.19 cm) and ST-3 6 grou p (2.35±0.11 cm) were smaller than that of control group (2.69±0.22 cm) . In de layed general polyarthritis rats of these two moxibustion groups, the number o f inflammatory nodes at the ear and tail was less than that of control group. 2) Reducing release of inflammatory mediators: Following moxibustion, the elevated contents of blood tumor necrosis factor (TNF) and IL-1 in AA rats decreased sig n ificantly compared with control group ( P <0.05). Moxibustion could also reduc e th e release of histamine and 5-HT. 3) Modulating free radical metabolism: In AA r a ts, moxibustion could reduce blood nitric oxide (NO) and malondiadehyde levels a nd raise SOD activity. 4) Regulating immunofunction: Moxibustion could cause th e endogenous IL-2 level thymus index to raise and reduce the secretion of IL-1 o f the over-excited megoxycytes and lower the spleen index in AA rats. Following removal of the adrenal gland or sympathetectomy (with 6-OHDA), the effects of m o xibustion in modulating immunofunction were weakened significantly. 5) Regulatin g central neurotransmitter level: In AA rats, following moxibustion, the elevate d NE and 5-HT contents in the hypothalamus were upregulated, while the elevated NO level was downregulated. 6) Involvement of hippocampus and pineal gland in th e effects of moxibustion: In AA rats, after injection of 6-OHDA for destruction of adrenagic nerve, the effect of moxibustion on reduction of inflammatory respo nse of the ankle was weakened. In addition, the decreased level of melatonin (MT ) of the pineal gland tissue in AA rats was raised by moxibustion. In a word, mo xibustion induced anti-inflammation and modulation of immunofunction may contri bute to its therapeutic effect in improving rheumatoid arthritis.
摘要:本文手检近 10年来刊登在国内 2 3种期刊的论文共 174篇 ,其中具有代表性的论文 40余篇 ,施行各种手术 13 40 0余例 ,针刺复合麻醉方法有针刺复合局麻、针刺复合硬膜外麻醉、针刺复合全身麻醉 (静脉、吸入 )等。文献提示麻醉效果基本满足临床需求 ,麻醉方法日趋规范 ,结论更为严谨 ,并正在采用现代科研手段肯定针刺复合麻醉的优越性 ,有部分单位继续坚持临床选用In the present paper, the authors review advances about the clinical studies of acupuncture anesthesia in recent 10 years from 1) historical development; 2) acu puncture combined with administration of local anesthetics; 3) acupuncture combi ned with epidural anesthesia; 4) acupuncture combined with general anesthesia; a nd 5) appropriate stimulus parameters of acupuncture anesthesia. In conclusion, c ompound acupuncture anesthesia provides a new anesthetic measure for surgical op erations and has a definite analgesic effect and many advantages and should be s tudied further.
摘要:本文查阅头针治疗中风病的有关文献并结合课题组的研究成果 ,阐述了头针治疗中风实验研究中的一些新思路和方法 ,主要有 :①建立标准化的中风动物模型 ;②中医头穴线在动物模型上的模拟 ;③头针在动物模型上的针刺手法及刺激量 ;④头针治疗中风病的时机选择 ;⑤头针治疗中风的即刻效应 ;⑥头针治疗中风的机制及检测指标的选择。为头针治疗中风病的实验研究提供参考In the present paper, the authors review some related literature about new ways of thinking and methods for experimental studies on scalp-acupuncture treatment of stroke from A) establishing standardized animal model; B) imitating human's scalp-points in animals; C) using suitable manipulations and proper stimulating quantities; D) selecting appropriate therapeutic opportunity; E) studying th e im mediate effect of acupuncture on stroke; F) the underlying mechanisms of scalp- acupunc ture in improving stroke and some appropriate indexes for evaluating the therape utic effects of acupuncture; and G) key points for attention. Currently, 1) elec tric coagulation method induced middle cerebral artery occlusion is a reliable c erebral ischemia model; 2) experimental studies have been carried out mainly on rats, but primate is most suitable for researching the mechanisms of acupuncture the rapy in treating stroke; 3) working out a united standardized scalp-point lines in different animals is necessary; 4) the most suitable stimulating quantity and parameters should be made clear earlier; 5) administ ration of acupuncture t hera py for stroke in the early stage and rehabilitation stage is applicable; and 6) studying the mechanisms of acupuncture in relieving stroke from cellular and mol ecular levels and intranuclear episodes is the conventional way at present.