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中国中医科学院针灸研究所,北京100700
Received:12 May 2025,
Revised:2025-07-16,
Online First:06 May 2026,
Published:25 May 2026
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苏雨昕,王佳,王雨晴,等.双光子显微成像技术在针灸研究中的应用前景[J].针刺研究,2026,51(5):663-668.
SU Yu-xin,WANG Jia,WANG Yu-qing,et al.The application prospects of two-photon microscopy imaging technology in acupuncture research[J].Acupuncture Research,
苏雨昕,王佳,王雨晴,等.双光子显微成像技术在针灸研究中的应用前景[J].针刺研究,2026,51(5):663-668. DOI: 10.13702/j.1000-0607.20250490.
SU Yu-xin,WANG Jia,WANG Yu-qing,et al.The application prospects of two-photon microscopy imaging technology in acupuncture research[J].Acupuncture Research, DOI:10.13702/j.1000⁃0607.20250490.
双光子显微镜凭借其深层组织穿透能力、低光毒性、高空间分辨率、抗光散射特性及长工作距离等优势,已成为活体组织、深层结构及透明化处理厚组织成像的理想工具,并广泛应用于生物医学研究领域。然而,该技术在针灸研究中的应用尚处于起步阶段。鉴于双光子显微成像技术在针灸研究中的巨大潜力,本文系统阐述双光子显微成像技术的原理与技术优势,并综述其在针灸领域的最新应用进展。同时,通过整合二次谐波生成成像、光遗传学、基因编辑及组织透明化技术,提出面向针灸研究的创新融合策略。并对如何利用双光子显微镜获得高质量成像提供具体建议。以期推动该技术在针灸研究中的深度应用,实现针灸效应的在体实时动态观测,为揭示针灸作用机制提供有力的技术支持。
Two-photon microscopy, with its exceptional capabilities in deep tissue penetration ability, low phototoxicity, high spatial resolution, anti-light scattering properties, and long working distance, has become an ideal tool for imaging living tissues, deep structures, and clearing processing of thick-tissue samples, and is widely used in the field of biomedical research. However, the application of this apparatus in acupuncture research is still in its early stage. Given the great potential of two-photon microscopy imaging in acupuncture research, this article systematically explains the principles and technical advantages of two-photon microscopy, and reviews its latest application progress in the field of acupuncture and moxibustion. At the same time, by integrating second harmonic generation imaging, optogenetics, gene editing, and tissue clearing techniques, innovative strategies tailored for acupuncture and moxibustion research are proposed. Concrete suggestions are also provided for achieving high-quality imaging with two-photon microscopy. These efforts aim to facilitate the advanced application of this technology in acupuncture and moxibustion research, enable real-time dynamic in vivo observation of acupuncture effects, and provide robust technical support for elucidating the underlying mechanisms of acupuncture and moxibustion.
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