摘要:50 Cases of neolarynx reconstruction under acupuncture anesthesia combining with drugs were performed from 1992 to 1995. We used the ear points " Lung", "Shenmen", "Sympathetic", body points " Hegu", " Zhigou", "Futu". The patients were given pethidine, rotundine, metoclopramide imtramuscularly 15~25 min before operation. The excellent rate was 98%
摘要:The function of red nucleus (RN) was important in motor control. This work was to study whether the RN influenced the effect of EA and the somatosensory afferent system. C-responses of spinal dorsal horn neurons(SDHN) were recorded as nociceptive responses- Electrical stimulation of RN could intensify the inhibitory effect of EA and inhibit nociptive reponse of SDHN. Naloxone(NX) could completely block that inhibition of RN- The result inferred that the RN can modulate both of somatomotion and somatosense. The endogenous opiate system is involved in the somatosensory modulation of RN
摘要:Experiments were performed on 33 white .male rabbits anaesthetized with chloralose and urethane under artificial venti1ation. The resu1ts were as follows: (1) depressor response could be evoked by electric stimulation of caudal ventrolateral medu1la (cVLM). The inputs of deep peroneal nerve (DPN)could inhibit partly the depressor response evoked by electric stimulation of cVLM (P<0.001 ). The regulation effects of DPN disappeared in 25~30 min. (2) after iv naIoxone (1. 5mg/kg) or microinjection of naioxone into rostral ventro1ateral medulla (rVLM) (2μg/1μl NS), the inhibitory effect of DPN inputs was abolished partly(P<0. 01, P<0. 001 ).These results suggest that: (1 ) the depressor response elicited by cVLM excitation in rabbits can be regulated by inputs of DPN; (2) opiate peptide in rVLM may take part in the inhibitory effect of DPN inputs on depressor response excited by cVLM
摘要:Experiments were performed on 53 rats. The nociceptive discharges of spinal dorsal horn neurons were extracellularly recorded by glass microelectrodes and tested for the effects of angiotensin Ⅱ(A Ⅱ ) on the nociceptive discharges of spinal dorsal horn neurons and AII on the inhibitory effects of electroacupuncture (EA ). The results showed as follows: (a) The nociceptive discharges of spinal dorsal horn neurons might be inhibited (25/35) or facilitated (10) with AⅡ (50~ 500ng/20ul) applied to the surface of dorsal spinal cord (L2-3 ); (b)The facilitory effects (5/5 ) were observed with A E (2ug/20ul)applied to the surface of dorsal spinal cord; (c) The inhibitory effects of EA (20Hz, 2 - 4V, 5min) could be increased with A Ⅱ (250mg/20ul, 8/9 ). Therefore, A Ⅱ may modulate the nociceptive response of spinal dorsal horn neurnons and influence the effects of EA, and different effects can be produced when the different dosage of A Ⅱ is applied to the surface of dorsal horn
摘要:The dynarnic changes of ACh activity in the spinal dorsal horn of rats were recorded by ACh ion selective microelectrodes. The influences of applying capsaicin to the spinal surface, adm1nistering anti - SP surum to the interior of dorsal horn, cutting of f dorsal roots and electroacupuncturing at "points" on the ACh activity were studied. The results showed that electroacupuncture at "points" and capsaicin could facilitate ACh release of the spinaI dorsal horn, it was fully inhibited by cutting off dorsal roots or obviously inhibited by administering anti-SP surum, The above results indicated that ACh rnight participate in the prirnary afferent of painsensation and acupuncture sensation as a neurotransmitter and SP might adjust the relese of ACh in spinal dorsal horn
摘要:Influence of thoracic spinal subarachnoid microinjection of noradrenaline (NA, 40mg/20ul) on the effect of electroacupuncture (EA) at Neiguan (PC6) was observed in 40 rabbits anesthetized with urethan-chloralose. Results showed that: 1) EA of Neiguan (PC6) could significantly accelerate the recovery of ST - segment and T-wave of electrocardiogram induced by acute myocardial ischemia (AMI); 2 ). Subarachnoid microinjection of NA also could promote the recovery of ST, T and blood pressure of post-AMI; and 3) Subarachnoid microinjection of NA could strengthen the effect of EA of Neiguan (PC6) to promote the reocvery of ST and T of Post-AMI. It suggests that a-receptors of intra-thoracic spinal cord participate in the action of EA of Neiguan (PC6) ameliorating AMI, and the upper thoracic segement is one of the links fqr connecting Neiguan and the heart
摘要:Acute myocardial ischemia of rabbit was caused by Pituitrin (2. 5u/kg)intravenously. The effect of electroacupuncture (EA ) at Neiguan polnt on transmernbrane potential was observed with a suspension microelectrode technique in situ and under the condition of natural respiration; the blood pressure and electrocardiogram were recorded simultaneously- The results showed that during acute myocardial ischernia, the resting potential (RP), action potentail amplitude (APA ) and maximurn velocity of depolarization (Vmax ) were all significantly decreased, but action potential duration (APD) was markedly prolonged and after depolarization potential appeared at the sarne time. All of the above mentioned changes of potentials could cause arrhythmia t while EA at Neiguan could weaken the changes of the potentials rnarkedly,prevent the ventricular cells from changing to slow reaction cells and reduce the incidence of after depolarization potential, That may be a reason why EA at Neiguan can prevent and cure arrhythmia complicated by acute myocardial ischemia
摘要:The new technique, NMR (nucleaf magnetic resonance) spectrum analysis was used in this research to measure the state of energetic metabolism of hepatic cells at the organic level. The experimentai results demonstrated that moxibustion on Guanyuan (CV4 ) point could remarkably increase the ATP molecules in the hepatic cells, significantly raise the therrnodynamic reserve and phosphate potential of the hepatic cells, so as to enhance the functional activities of liver. It is discussed here of the mechanism of anti-tumor by moxibustion treatment and of the theory of warming the kidney and enhancing the Yang to tonify to energetic Qi by moxibustion on Guanyuan point
摘要:Many factors, such as the selection of acupoints, the interval between acupunctures, the acupuncture time, the manipulation and the specification of acupuncture needles, are capable of affecting curative effects, There were some studies on these factors ago. However each of these studies only related to a single factor. These stlldies could not reflect the clinical truth, and could not provide accurate overall data for rnedical staff. ln order to solve the above problems, we carried out an animal experiment designed by L9 (34) orthogonal design. The experiment which consisted of 9 acuouncture groups and 3 control ones was carried out in lO8 rats, which were suffered from experirnental gastric ulcer. In the experiment, the body weight, ulcer area, plesrna SoD and LPO of each rst were observed.The results showed that in the acupuncture treatments for experimental gastric ulcer in rats, 10 minutes' electric acupuncture treatment using thinner needles and the method that each rat was treated every other day were the best methods. The reason why the above results came into being was elucidated in this paper
摘要:18 rats were randomly deivided into 3 groups: control grup . the group merely with experimental gastric ulcer and the group with experimental gastric ulcer after 3-week moxibustion. The results showed: (1 ) moxibustion could significatly reduce the ulcer area of the rats (P<0. 05); (2 ) compared with control group, the experimental gastric ulcer model rats had an increase in copper content and Cu/Zu ratio, the differences were statistically significant (P<0. 05, P< 0. 01 ); (3 ) in contrast to the rats merely with experimental gastric ulcer, moxibustion-pretreated rats had a significant increase in Zinc content in serum (P<0, 01 ), but its copper content and Cu/Zn ratio in serurn were significantly decreased (P< 0. 05, P< 0. 01 ). The results confirmed that moxibustion pretreatment at "Shen Jue'could protect the gastric mucosa from strass-induced uleer, having protective effect on gastric mucosa. The results also suggested that there was a disbalance .in trace elements metabolism, but moxibustion could improve it. This was most likely to be one of the mechanisms of moxibustion protection on gastrip mucosa
摘要:The purpose of this study is to observe the effect of electroacupuncture at "Shenshu" point (U. B. -23 ) on the renal blood flow (RBF ) under the conditions of normal, glyceroLinduced renal ischemia and renal neurotorny. The RBF, which is measured by hydrogen gas clearance method, was chosen as index. The results are as follows:1. RBF is decreased by electroacupuncturing "Shenshu" point under both conditions of normal and glycerol-induced renal ischemia.2. After renal neurotomy, RBF is increased by electroacupuncturing "Shenshu"point.These facts suggest that the effects of electroacupuncture at "Shenshu" point relate to the renal nerve and body fluid
摘要:The electric discharges of the post-pelvic ganglionic vesical nerve fibers were recorded to investigate the effect of acupuncture on the function of urinary bladder. It has been dernonstrated that the changes of the pressure-volume of urinary bladder are controlled not only by the wall of the uinary bladder its self, but also by the regulation of parasympathetic nerve; the e1ectro-acupuncture can play an role of regulation in the functional state of urinary bladder through nerves
摘要:The present article reports EA with special acupoints may promote the enlargement of bilateral adrenals and enhance the levei of blood corticosterone in ovariectomized (OV) rats. The rnean of adrenal weight (M± SE, mg) as following, OV + EA group 71 ± 2. 1, OV group 57. 4 ± 4. 7, EA group 57. 6 ± 1. 1 and control group (CT group) 54 ± 2. 8 respectively. For observation of a self control, the detection of blood corticosterone contents was performed in the same animals before and after ovariectomy. The results showed that before ovariectomy the contents of blood corticosterone in the rats were 3. 27 f 0. 7μg/100ml before EA and 3. 63 ± 0.9μg/100ml after EA. No significant difference of blood corticosterone level was found before and after EA. After the animals ovariectomized the level of blood corticosterone increased from4. 38 ± 0. 62μg/100ml to 6. 02 ± 0.77μg/ 100ml following EA treatment (P<0. 01 ). No similar effect of EA treatment with control acupoints in OV rats was seen. These results suggest that EA may regulate the ahnormal function of endocrine and this effect of EA possesses relative specificity of acupoint
摘要:Our previous study suggested that peripheral sympathetic nerve played an important role in the electroacupuncture (EA)-induced irnrnunomodulation. The aim of this study is to explore the role of peripheral parasympathetic nerve in it. All mice are preimmunized with SRBC (sheep red blood cell) 4 days before experiment. The animals in different groups were injected i. p. with Hemicholine-3 (HC-3, an ACh synthesis blocker) or physiological saline 3 hr before EA. The main results are as follows: 1. In saline group, the LTT (lymphocyte transformation test), IL-2 (interleukin-2 ) of spleen and lymphocyte number of thymus are rnarkedly increased, and the other immune parameters have no change after EA.2. In HC-3 control group, the LTT, IL-2 are decreased significantly and the other immune parameters have not shown influence.3. In the HC-3 with EA group, the magnitude of LTT, lL-2 did not significantly change as compared with HC-3 alone, it is especially noteworthy that the other unchanged parameters by HC-3 such as spleen weight, spleen index, thymus weight, thymus index, number of spleen cells, spleen LZM(lysozyrne) and thymus LZM are clearly decreased after EA.The above results suggest that peripheral parasympathetic nerve may play some prornoting effect in the EA-induced immunomodulation. Its effect may be mediated by ACh released from parasympathetic nerve endings
摘要:We have made two kinds of experimental arrhythmia of rabbits by injecting aconitine and stimulating hypothalamus. Acupuncture could improve arrhythmia, after Ca++ in Neiguan(PC6) was chelated with EDTA solution the effect of acupuncturing Neiguan(PC6) was abolished. It indicates that Ca++ may be the key factor of acupuncture effect and one of the important material bases of the functional activity of meridians and collaterals
摘要:We studied mucosal SIgA immunity of patients with allergic asthma treated by acupuncture. Our results showed that concentrations of SIgA and total IgA in saliva (P<0. 01, <0. 02) and in nasal secretions (P<0.02,<0.02), and levels of IgE in sera (P< 0. 001 ) from patients with allergic asthma were significantly decreased after treatment with acupuncture. The results suggested that attacks of delayed and immediate allergic asthma could be effectively inhibited by acupuncture. The facts that there were no significant changes in IgA in supernatenats of peripheral blood lymphocytes stimulated with PWM of patients with allergic asthma after treated by acupuncture were associated with regulation of negative feedback of T-lyrnphocyte expressing IgA-R and it released bound factor of IgA in production of polymeric IgA
摘要:In this article, the clinical 31 cases patients with Rheumatoid artritis (RA) were observed and animal experiments were carried out on RA model rats and the treatrnent of acupuncture with combination of the different points was adopted. It was found that most of the 31 cases of RA patients had the signs of bloods stasis and abnormal hemorheology, statistical analysis indicated that there were significant differents between RA patients and healthy person- Red and swelling in joints of four limbs, ear and tail, clubbed toes,abnormal hemorheology, high in blood platelet aggregation and so on were found in model rats of RA, statistically, sinificant differents also exist between model rats of RA and healthy rats. The above indesexes in patients and model rats of RA were significantly improved after acupuncture treatment, which suggested that (1) there be a close relation between RA and blood stasis. (2) acupuncture play a role of promoting blood circulation to rernove blood stasis.(3) examination of hemorheology may be one of standards that development of RA and curative effect
摘要:In this paper, the principle of a confocal laser scan microscope system and its advantages are introduced. Its applications in biology and research of acupuncture and meridian are also introduced. Comprared with conventional microscope, the confocal laser scan microscope system has many advantages such as high resolution fluorescent irnage, 3-dimension reconstruction, images quatitative analysis of concentration of ion within a cell, cell-cell communication and so on. It will offer many mathods for researching acupuncture and meridian